ISPa11
- Family IS110
- Group IS1111
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AE004697 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa PA01 |
DNA section
IS Length : 1372 bp
Ends
IR Length : 13
IRL : caatgaaATGGACTCCTCCCTCCTACGGCGTCAAGCGCCAGACTAATGGT
IRR : ----ataATGGACTCCTCCCGGTTTGCCAGTGTTTGTCTTGATT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|
DNA sequence
CAATGAAATGGACTCCTCCCTCCTACGGCGTCAAGCGCCAGACTAATGGTGCAGCCACAAGTCCCGGAGGGGGAGTCAGCATGAACCTTAGTCGCATTGG
TCTGGATCTGGCAAAACAAGTATTCCAGGTGCACGGCGTTGATCGTCACGAGCATGTGGTATGTCGTCGCCAACTCAAGCGGGCACAGGTGCGGGATTTC
TTTCGCCAACTGCCGCCGTGCCTGGTGGCGATGGAGGCCTGCGGCAGTGCGCACTACTGGGCGCGCGAGTTGCGGGAGCTGGGCCACACGGTACGCCTGA
TCGCACCGCAGTTCGTAAAACCCTACGTCAAGGGTGACAAGCACGATGCACACGACGCCGAGGCGATCTGCGAAGCCGCCAGTCGTCCGAGCATGCGCTA
TGTCCCGGTGAAGAGCGCAGAGCAGCAGGCCGTGCAGTCGATGCATCGGGTGCGCAGTCGTCTAGTGCGGGCCCGCACGGCGTTGTGCAATGAGGTGCGT
GGCTTGCTGGGTGAGTTCGGGCTGATCGCGACTCGACGTGGGCGAGCGGCGACGATGGCCTTGCTCGAAACGGTCATGGCTACCGAGCCGGCGCCCTTGC
CGGCCCCGATGGGCGAGCTGTTGCGTGAATTGAAAGATGAATTGCAGACGCTGGAGGCGCGCATCGCTCGACTGGAGCGACAGATTCAGGCTCACGTACG
TGGCGATGCCCGCATCCAGCGCCTGCTGGCGGTGGAAGGCATCGGCCCGATCAGCGCCAGCGCGGTGGCGGCATCCGCCGGTGATGCACGGCAATTCCGC
ACGGGCCGCCAGTTTGCGGCCTGGCTGGGCCTGGTGCCACGGCAGCACTCCACGGGCGGGCAGCAGCGCTTGGGCAACATCAGCAAGCGCGGCGATACCT
ACTTGCGGACCTTGCTCATCCATGGCGCCCGTGCAGTCGTGCGCTGTTGCGCCAACAAGACCGATGCCCGCAGCCGCTGGCTGCAAGGTCTGCTGCAACG
GCGTCCTGCCAATGTCGTCGCCGTTGCCCTGGCCAACAAGAATGCCCGGATCCTTTGGGCTTTACTCAGCCGGGAGACATGCTACCGGCCCGGTTGAGCG
TTCCTGCCACACCGTAGTTGAAACATCCACCACGATTGCTCAGTGAATGACAATGATGACGAACCGGTCGAACCGGCCTGCATGAAACCTGGTTTATACG
TGGGCTCCCTGCTGCAGTGAAGCAAAGCCGTTAGGGCGATCAGGTATGCAGGCGCGCATTTCATCAGGGCTCGGGAGTTGCAACACCACTCCATGAAGCC
GGATATACGGATGCAGTCGTACACAGGTTTGAAATCAAGACAAACACTGGCAAACCGGGAGGAGTCCATATA
TCTGGATCTGGCAAAACAAGTATTCCAGGTGCACGGCGTTGATCGTCACGAGCATGTGGTATGTCGTCGCCAACTCAAGCGGGCACAGGTGCGGGATTTC
TTTCGCCAACTGCCGCCGTGCCTGGTGGCGATGGAGGCCTGCGGCAGTGCGCACTACTGGGCGCGCGAGTTGCGGGAGCTGGGCCACACGGTACGCCTGA
TCGCACCGCAGTTCGTAAAACCCTACGTCAAGGGTGACAAGCACGATGCACACGACGCCGAGGCGATCTGCGAAGCCGCCAGTCGTCCGAGCATGCGCTA
TGTCCCGGTGAAGAGCGCAGAGCAGCAGGCCGTGCAGTCGATGCATCGGGTGCGCAGTCGTCTAGTGCGGGCCCGCACGGCGTTGTGCAATGAGGTGCGT
GGCTTGCTGGGTGAGTTCGGGCTGATCGCGACTCGACGTGGGCGAGCGGCGACGATGGCCTTGCTCGAAACGGTCATGGCTACCGAGCCGGCGCCCTTGC
CGGCCCCGATGGGCGAGCTGTTGCGTGAATTGAAAGATGAATTGCAGACGCTGGAGGCGCGCATCGCTCGACTGGAGCGACAGATTCAGGCTCACGTACG
TGGCGATGCCCGCATCCAGCGCCTGCTGGCGGTGGAAGGCATCGGCCCGATCAGCGCCAGCGCGGTGGCGGCATCCGCCGGTGATGCACGGCAATTCCGC
ACGGGCCGCCAGTTTGCGGCCTGGCTGGGCCTGGTGCCACGGCAGCACTCCACGGGCGGGCAGCAGCGCTTGGGCAACATCAGCAAGCGCGGCGATACCT
ACTTGCGGACCTTGCTCATCCATGGCGCCCGTGCAGTCGTGCGCTGTTGCGCCAACAAGACCGATGCCCGCAGCCGCTGGCTGCAAGGTCTGCTGCAACG
GCGTCCTGCCAATGTCGTCGCCGTTGCCCTGGCCAACAAGAATGCCCGGATCCTTTGGGCTTTACTCAGCCGGGAGACATGCTACCGGCCCGGTTGAGCG
TTCCTGCCACACCGTAGTTGAAACATCCACCACGATTGCTCAGTGAATGACAATGATGACGAACCGGTCGAACCGGCCTGCATGAAACCTGGTTTATACG
TGGGCTCCCTGCTGCAGTGAAGCAAAGCCGTTAGGGCGATCAGGTATGCAGGCGCGCATTTCATCAGGGCTCGGGAGTTGCAACACCACTCCATGAAGCC
GGATATACGGATGCAGTCGTACACAGGTTTGAAATCAAGACAAACACTGGCAAACCGGGAGGAGTCCATATA
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1017 bp | 338 aa | 81 | 1097 | + | No |
Chemistry : DEDD
ORF sequence :
MNLSRIGLDLAKQVFQVHGVDRHEHVVCRRQLKRAQVRDFFRQLPPCLVAMEACGSAHYWARELRELGHTVRLIAPQFVKPYVKGDKHDAHDAEAICEAA
SRPSMRYVPVKSAEQQAVQSMHRVRSRLVRARTALCNEVRGLLGEFGLIATRRGRAATMALLETVMATEPAPLPAPMGELLRELKDELQTLEARIARLER
QIQAHVRGDARIQRLLAVEGIGPISASAVAASAGDARQFRTGRQFAAWLGLVPRQHSTGGQQRLGNISKRGDTYLRTLLIHGARAVVRCCANKTDARSRW
LQGLLQRRPANVVAVALANKNARILWALLSRETCYRPG
SRPSMRYVPVKSAEQQAVQSMHRVRSRLVRARTALCNEVRGLLGEFGLIATRRGRAATMALLETVMATEPAPLPAPMGELLRELKDELQTLEARIARLER
QIQAHVRGDARIQRLLAVEGIGPISASAVAASAGDARQFRTGRQFAAWLGLVPRQHSTGGQQRLGNISKRGDTYLRTLLIHGARAVVRCCANKTDARSRW
LQGLLQRRPANVVAVALANKNARILWALLSRETCYRPG
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
There are 6 copies of this element in the P. aeruginosa PA01 chromosome, with the transposases corresponding to orfs PA0445, PA2319, PA2690, PA3434, PA3993 and PA479.
The transposase protein is 47% identical to that of IS1111.
ISPa11 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
There are 6 copies of this element in the P. aeruginosa PA01 chromosome, with the transposases corresponding to orfs PA0445, PA2319, PA2690, PA3434, PA3993 and PA479.
The transposase protein is 47% identical to that of IS1111.
ISPa11 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Stover et al. (2000) Nature 406, 959-964
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384