ISPa11

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AE004697 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa PA01
DNA section
IS Length : 1372 bp

Ends


IR Length : 13

IRL : caatgaaATGGACTCCTCCCTCCTACGGCGTCAAGCGCCAGACTAATGGT
IRR : ----ataATGGACTCCTCCCGGTTTGCCAGTGTTTGTCTTGATT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

CAATGAAATGGACTCCTCCCTCCTACGGCGTCAAGCGCCAGACTAATGGTGCAGCCACAAGTCCCGGAGGGGGAGTCAGCATGAACCTTAGTCGCATTGG
TCTGGATCTGGCAAAACAAGTATTCCAGGTGCACGGCGTTGATCGTCACGAGCATGTGGTATGTCGTCGCCAACTCAAGCGGGCACAGGTGCGGGATTTC
TTTCGCCAACTGCCGCCGTGCCTGGTGGCGATGGAGGCCTGCGGCAGTGCGCACTACTGGGCGCGCGAGTTGCGGGAGCTGGGCCACACGGTACGCCTGA
TCGCACCGCAGTTCGTAAAACCCTACGTCAAGGGTGACAAGCACGATGCACACGACGCCGAGGCGATCTGCGAAGCCGCCAGTCGTCCGAGCATGCGCTA
TGTCCCGGTGAAGAGCGCAGAGCAGCAGGCCGTGCAGTCGATGCATCGGGTGCGCAGTCGTCTAGTGCGGGCCCGCACGGCGTTGTGCAATGAGGTGCGT
GGCTTGCTGGGTGAGTTCGGGCTGATCGCGACTCGACGTGGGCGAGCGGCGACGATGGCCTTGCTCGAAACGGTCATGGCTACCGAGCCGGCGCCCTTGC
CGGCCCCGATGGGCGAGCTGTTGCGTGAATTGAAAGATGAATTGCAGACGCTGGAGGCGCGCATCGCTCGACTGGAGCGACAGATTCAGGCTCACGTACG
TGGCGATGCCCGCATCCAGCGCCTGCTGGCGGTGGAAGGCATCGGCCCGATCAGCGCCAGCGCGGTGGCGGCATCCGCCGGTGATGCACGGCAATTCCGC
ACGGGCCGCCAGTTTGCGGCCTGGCTGGGCCTGGTGCCACGGCAGCACTCCACGGGCGGGCAGCAGCGCTTGGGCAACATCAGCAAGCGCGGCGATACCT
ACTTGCGGACCTTGCTCATCCATGGCGCCCGTGCAGTCGTGCGCTGTTGCGCCAACAAGACCGATGCCCGCAGCCGCTGGCTGCAAGGTCTGCTGCAACG
GCGTCCTGCCAATGTCGTCGCCGTTGCCCTGGCCAACAAGAATGCCCGGATCCTTTGGGCTTTACTCAGCCGGGAGACATGCTACCGGCCCGGTTGAGCG
TTCCTGCCACACCGTAGTTGAAACATCCACCACGATTGCTCAGTGAATGACAATGATGACGAACCGGTCGAACCGGCCTGCATGAAACCTGGTTTATACG
TGGGCTCCCTGCTGCAGTGAAGCAAAGCCGTTAGGGCGATCAGGTATGCAGGCGCGCATTTCATCAGGGCTCGGGAGTTGCAACACCACTCCATGAAGCC
GGATATACGGATGCAGTCGTACACAGGTTTGAAATCAAGACAAACACTGGCAAACCGGGAGGAGTCCATATA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1017 bp338 aa811097+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MNLSRIGLDLAKQVFQVHGVDRHEHVVCRRQLKRAQVRDFFRQLPPCLVAMEACGSAHYWARELRELGHTVRLIAPQFVKPYVKGDKHDAHDAEAICEAA
SRPSMRYVPVKSAEQQAVQSMHRVRSRLVRARTALCNEVRGLLGEFGLIATRRGRAATMALLETVMATEPAPLPAPMGELLRELKDELQTLEARIARLER
QIQAHVRGDARIQRLLAVEGIGPISASAVAASAGDARQFRTGRQFAAWLGLVPRQHSTGGQQRLGNISKRGDTYLRTLLIHGARAVVRCCANKTDARSRW
LQGLLQRRPANVVAVALANKNARILWALLSRETCYRPG

 

Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.

There are 6 copies of this element in the P. aeruginosa PA01 chromosome, with the transposases corresponding to orfs PA0445, PA2319, PA2690, PA3434, PA3993 and PA479.

The transposase protein is 47% identical to that of IS1111.

ISPa11 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Stover et al. (2000) Nature 406, 959-964
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384