ISfinder Mirror Site

ISOpb1

Family IS1
Group ISMhu11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
ABEA01000009	ND	Opitutaceae bacterium	Opitutaceae bacterium TAV2

DNA section

IS Length : 4054 bp

Ends

IR Length : 65/67

IRL : GGTATTCCCTCAATTTCGATCTACCAAACAATCAACAACTTACAAAGCTC
IRR : GGTATTCCCTCAATTTCGATCTACCAAACAATCAACAACTTACAAAGCTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGGTAAAGAAGTATT	CACTGGCT	GGATACTTTTTTCTC	8

DNA sequence

GGTATTCCCTCAATTTCGATCTACCAAACAATCAACAACTTACAAAGCTCTCCAGTCGTAAGGATTTCGCCCATTGTTTAGTGACAATCTTGTCCATTAT
TTCTACACCAATTTCACATTGAGAGTTTTTAATTGTAAGAAATTTCATGGATAAAAATAAAAATTTCTTCTCAAAAACTCATGGACTCAACCTCAGTCGC
ATACTTTTCATAGAAAAACGTCATGAATATCCTGATTTTTACTGGGTTTATTTCGATACCGGGTTAGTGGTCTCTATTACCAATACAGAATATGAAGATA
TAACACAATATATCACCGCATTATAAATGGGCGAAAGCCAACTGACTTATAGGATTCGACCTCAATTAGTTTATATTCAACAATAGAACCATCTTCTAGT
GTAAAACGCAAGAAGCTGTCTATTTCTTCTGCTTTCCCGGAATGTTGTTTTATTTCGCCCGAAGTAAGCTTTACTTCGTAATTTATTACTATTTGCTGCG
TCATTTTGTCTATTGGTTTATTTTTCGGTTAGCGAATCTTAAACTAAAATTCTCGCACAATAGCACCTTGTAGTCAAGCAATTAAATTTCGTAACTTATT
ATTGGAGCTACGAAGGCGGGCTTCTTGGCTTCTGTAATGGATTCATAAATATTTTTATATTGGCGGGCTTTAGCGGTGTCTCAGAAACGCCAATCTTCGC
AGAATATGATTCTATTGTCAATGGTCTTTATTCCATAACTTATTGATTTTAAATTAAAAAGACAGAAAAATAAAAATAAAGTTCAGTGATTTTATTTATT
TTTCAAAACAAGTTGATTGAAAATAAAGACCAGCATTATTTTTAATTTCCCTCACCTATTAAAATATATTGATCCGATCTTCCCCGAAATAATTCATTCC
CCTTTTCTATCAGATTTTGCTTGACCGATCAAGGATAAAATGACAGTTTCATTCATTATGCCAAAGAAAGATTTCAGTCAAAATGCCCTCTCCATTGTGG
AGTTGGTCACTGGTGAGAAAATTGCCAAAAATTCAGCCGCCGTTTCCCTCGGACGATTGGGCGGACTAAAAGGCGGGAAGGCCCGCGCACTCAAATTAAC
AGGCGAAGAACGCAGTGCCATTGCGAAAAAGGCAGCATTAAGTCGATGGAAGAAAAAGCAAAATGAGGGATTAGATTTTAGTAAAAAACAGAAAAGGAAT
ATTTCAGAATCATAAATAATTTTTAAATAATAGGTTAAGTCATATTTTATTCCCCACTTTTTTCTTGCTATCCGTGACCGCTCAAGCATAATCGGGACCA
TGAACCGATTGGCACATTCAAAGCGAGTTCAGATGCTTTCCTGCCTTGTGGATGGATGCAGCATGGAAGCAACGGCCAGAATCGTGCATGTTGCGCCCAA
CACAGTTGCAAAATTTTTAGTGGATTTGGGAAAAGCCTGCACGCGGTATCAAGCCCGTGTCTTTGAAAATATTTTCTGTAAGCGAATTCAATGCGATGAA
ATTTGGTCTTTTGTCGGATGCAAAGAAAAGAACGTAACAAACAACGGAAAACGGCAGGGCGATACTTGGACATGGATTGCTTGCGACCCTGACACAAAAT
TAGTTCCTTGTTGGTTTATCGGGCGTCGGGATTCTGAATCTGCTAAAAAATTCATGAGGCGACTTGCCCGCCACTTATCACTAGGCAGCACTCAAATTAC
CACCGATGGATTAAAAGCCTATATCAATGCCATTAAAGAAATATTATGGATTGAAACCAGTTATGGCATGGTTGAGAAAAAATACGATGTCAGTGGAGAC
GATCACAGAACCAGATATATCGGGAGCGAAAAGACGGCAATTTTTGGAAACCCCGACCCCGACACAATGAATACCTCCATCGTGGAACGGCAAAATTTGA
CCATGAGAATGTCAATGCGCCGCTTCACTCGAAAAACAAATGGCTACTCCAAAAAAATCGCAAACCACCGTTATGCTATCGCCTTGCATTTTATGTATTA
CAACTTCGCCAGAATTCATTCCACATTGAACACAACCCCAGCAGTAGCCGCAGGAATTGCAAATCATGTTTGGAGTTTGGATAAGATTCTGAAACTTATT
GAAAGATAGGTAATTAACAATTCTCAATTGACTTAATCTTCTAATTTTACTTCATAGATTTCCTACAGTCTAATTTTCGCAGAAACTCGTTGATAAGTCT
CCCGTCTGAAAACCGCTAAATTTCTCTTGGGAGAGGACTTATTGATCCGAGCGCGTCACAACTGGCGCGGTTTGGTCATTCCTCTCCTAAGCTCTTAGCG
GTGCTTCAGACGGCGGGATGTTCTGGGCCGCGCCTCTTTATTTTTGGGTTTTTACGGAAGTTGTTCTGATCCAAACAAAAAAATAGAATCCGTCATGAAA
ACCGCTAAAAATAAAAATATAATTAAATTAATCCTTTTCCTTATTGGAATGGGATTCCTGACTGTTGGATGCGAAAAACAGCATTCTTCTTTTCAAGTGG
AAGATAACTCCCAAGACCCTACCGCTTTATTAAGAGCACGACTAACTAAATTAAAATCTTTCGCCATTGATTCAGATGCGAAGAACCCCGGAGCAAAGAG
TAGCATTTTTCCGAAAATATCTTACGATGTTAAGCAAACAAATTCAATGGTTTCTCCTTATATAGGAACAGTTGAAATCCACGAAATATCTTATGAGGTC
GATTCTAATGGGATCAACCGTGAAAAGGGATGGCGTGGTTTAAAAATTACATTAGCATTCCAGCAAAATGAATGGGTTGTGAAACAAGTTCGAGTGAAAA
CTTATACATCTTCTGATTTTCAACAATATATAGAGGACTACAGAGACGCTTATAATTCTGAGATTGCTCAGGCCAAAATAATACTTTGGATGTTTGGCGA
CTAACTTAATGTCAGATATAATACTCATAGAAATGCAAAAAAACATTTAAATTATTATGAAGCCCGAATACCAACAAATTTTAAAAATCATCTTTCTAGC
GGTTGTTGTCCCCGTTATGGGCCAAGCTGATGTAAAATATGCTCCTTTGCAGTCTAGCATAGTGAAGGTCAGTAATATTCGTAGAACGGCAGCAATCGAA
GGAGATTGGAAAGAAGGAATAGATGAAGCATGGAAAGATGCAAAGAAGAAAGCCTTTGAAAAAGCTCTTAATGCTTACAAAATCACCTCTCAGAGAATTG
GAGTAAAAGGAAAAATGAGGAGAGTTAAAATTACCATAGAATGGACAGAATAATCGCTACATCAAAAAAACAGTTTGTAAATAAAGATACTTGATATTTG
AGCCACATAAGATATTGAAATTCAATTTTCTGGCATCCCGCCAGAATCAGTAAAAACCATTAACCAAAAAATAAAGAAATAAAATGAAATTTGATCTAAA
ATTATCTGATACAGAAAAGCCAATTCATGAATCAGATGGGAAAAGATGTTTCTTCGTTGAAAAGATGGATATTAACAACATATCTTCCCATTTAATTAAG
CATAACATAATATTTACAAATGAAGGAATTTGTGCCACAAGTGAAATAGGGGAATTATATTACTTTGTATCAGATTCAAAGGAGGAAGATATAATGTTCG
CACTTGAAGATTGCATCAAATTACTCCGTCCATACCCAAAAGGTTAAGCCAGAGGGGTCGATTTTTTTAGTTTCGATTTCTTTTTGAATTACACTCAACG
CCATAGGAACAAAAGGACTAAGATTCTTGAATTTTCTTATCATTTTTGCATGGACATTTATCCATGAAACTATTTTACCGTCCTGATCCTTCGCGGCAAA
ACAGAGAAGATCATAAGACAAATTGAAGGGATTCAAAGAATCTAATATTATGCTAAAACCGTTATATTGAATTGGCACGCTCTTGGTGATATTATTAAAA
TTTGCATTCTTCATAAAAAAATTGTTTTAAATATTTTTTTGGCTTTCTTTTTTAAAAAGGAGAGGGGGATTTTTTTCTAAAATTACAAAATCATTACGAT
TGGAGAGCTTTGTAAGTTGTTGATTGTTTGGTAGATCGAAATTGAGGGAATACC

Protein section

ORF number : 5

ORF 1

Length		Begin	End	Strand	Fusion ORF
276 bp	91 aa	940	1215	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTVSFIMPKKDFSQNALSIVELVTGEKIAKNSAAVSLGRLGGLKGGKARALKLTGEERSAIAKKAALSRWKKKQNEGLDFSKKQKRNISES

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
810 bp	269 aa	1300	2109	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNRLAHSKRVQMLSCLVDGCSMEATARIVHVAPNTVAKFLVDLGKACTRYQARVFENIFCKRIQCDEIWSFVGCKEKNVTNNGKRQGDTWTWIACDPDTK
LVPCWFIGRRDSESAKKFMRRLARHLSLGSTQITTDGLKAYINAIKEILWIETSYGMVEKKYDVSGDDHRTRYIGSEKTAIFGNPDPDTMNTSIVERQNL
TMRMSMRRFTRKTNGYSKKIANHRYAIALHFMYYNFARIHSTLNTTPAVAAGIANHVWSLDKILKLIER

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
561 bp	186 aa	2344	2904	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGFYGSCSDPNKKIESVMKTAKNKNIIKLILFLIGMGFLTVGCEKQHSSFQVEDNSQDPTALLRARLTKLKSFAIDSDAKNPGAKSSIFPKISYDVKQTN
SMVSPYIGTVEIHEISYEVDSNGINREKGWRGLKITLAFQQNEWVVKQVRVKTYTSSDFQQYIEDYRDAYNSEIAQAKIILWMFGD

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
297 bp	98 aa	2957	3253	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKPEYQQILKIIFLAVVVPVMGQADVKYAPLQSSIVKVSNIRRTAAIEGDWKEGIDEAWKDAKKKAFEKALNAYKITSQRIGVKGKMRRVKITIEWTE

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
294 bp	97 aa	3621	3914	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKNANFNNITKSVPIQYNGFSIILDSLNPFNLSYDLLCFAAKDQDGKIVSWINVHAKMIRKFKNLSPFVPMALSVIQKEIETKKIDPSGLTFWVWTE

Blast result :

Comments

ISOpb1 is 40% aa similar to ISLysp1.
The transposase is the second ORF, others are passengers genes.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S., Schmidt,T. and Richardson,P.(2007) Direct submission GenBank.