ISfinder Mirror Site

ISNisp2

Family IS1595
Group ISNwi1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAMY01000002	ND	Nitrobacter sp.	Nitrobacter sp. Nb-311A

DNA section

IS Length : 2637 bp

Ends

IR Length : 20/24

IRL : GGCGATTGTGTAGTTTACAAACCCAGAAATCGTGGTAGTATCAACTTACT
IRR : GGCGATTATGCACTTTGCAAACCCTACGCGATTCACTTAGGTTGATGGAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTTACAACTC	TCCCGAGC	ACAAGAACATTAAGGGA	8

DNA sequence

GGCGATTGTGTAGTTTACAAACCCAGAAATCGTGGTAGTATCAACTTACTTACTGAAAACACTAGGTTTGTCATATGTCCGTTCTTTCCAAGAAGTATTT
CCACGATGAAGCAGCCGCATTCCGCCATCTAGAGAAATTGCTGTGGGGCAATGGGGTTATTTGCCCGAAATGCGGTGAGGTTGATCGCGCGGGTAGGCTG
GAGGGCGTGAGGGGCAAGAACGGCAAGGCTCGTCTGGGTCTTTGGAAGTGCTACGGCTGCCGGAAACAGTTCACGGTGCGCGTCGGTACGGTTTTCGAGT
CGGCGCATATTCCCCTGCATAAGTGCTTGCAGGCTGTGCACCTCATGGTGTCGAGCAAGAAGGGTGTTTCGTCCCATCAACTGCATCGCATTCTTGAAAT
TCAGTACAAGTCGGCGTGGTTTTTGGCGCACCGCATCCGTGAAGCGATGCGCGATGGCAAGCTCGCGCCGATGGGCGGCGAAGGCAAAATCATCGAGGCG
GACGAAACTTTCACGGGCCGTCTGGCCGGCCAGCCCAAGAATAAAAAAGGCGGTTGGGCTCATAAAAACGTGGTTCTAACGCTCGTGGAACGGGGCGGTA
TAGCCCGAAGCTTCCATGTCGATGGTACGCGCGTTGCGGATATTGTGCCGATCGTGCGAGCCAATATTCGCCGCGAAAGCAGTCTGATGACCGATGAGGC
TCGCCACTACATCAGCGTTGGTAAAGAGTTTGCCAGCCACGACAGTGTGAACCACAAAGAAGAGGAATACGTTCGCGGCAACGTCTCAACCAATACAGTT
GAGGGCTATTACAGCATCTTCAAGCGCGGCATGAAGGGTGTCTATCAGCACTGCTCGGAGAAGCATCTGCACCGTTATCTAGCAGAGTTCGACTTCCGGT
ATAGCAACCGCATTGCACTGGGCGTGGACGATCAGGATAGGGCGGACGAGGCCATAAGGGGAATGGTAGGCAAGCGCCTAACATATCGGGTCACTGACAG
CGCGGCGGCCCGGTAGATGGGAGAGGAAGGCGGCGCGAATCAGTGCAAGAGGTGAGCAGCTAGAATTCGGGTTCCCCCGGAGAGAAAAGTGATTCGCGGT
CAATGAGTTATGTAACACATTGATTCAACTTGCAGAATCATATATAAAATAGCGGGACTCCGGAAGGGGGGTCGCGAGATTTATCTCGCGGCGCTGGATG
GTACGCTTGGCACCACTAGAAGCTGGGCTATTGATCATCCGGAAAACGCCTCCGGGTCCCACCATTGTGTGGGGTATCCGTAGTATCGGAAGATTTCCGC
CTGATCCGAGTCTAGCTTCGCTTTAGAGAAGCTGGGCAATAGCTTAACTCCATAGCCAGATATCTTATCAGATTCCCTTGCCATGCGAGGGCGCAGCAGT
TTTGCGTATTCGGGAGCGCAACCGCTGGTGTCGTATTTGTCGCAAGCCTTGAAAAACGATGCAGCTACCACGTCGGCGAGCTGCAACCCTTCCCGCTCCA
AATGCGGATAGACGCGAAGTAAGTTCATATGCAGCACTTCGAAGCACAATTTACCCCAAGGTAGAAAAGGCTTGTTCCGCTTCATTCGAAGCCATTCATA
GTATGCGTGCATCTGATCATATCGGAGTCCACCACGCGCGCTGTACTCAACGCGAAGATATCGAGGTTCGCCAAAGTCCAGCATAGAGCGATATAAGACG
AACCGTGTAACGCGCTCCAATAGAACGCGAGTCATCCAACAATAAAACCAGCACTGCGATGGAATCTTCGCTGCATCAGGGTTCGTATAGCCTTCCATGT
TCTTCTTATTGGAAGCCACCACGAAACAGCGAAGTTCCATTTCTGAAATTTTCCTGCAGACGGCGACCTTATTGGGATGGGACAGATCGGCGAAATGTAA
TGCCTTTGCTTGCTTGCTACGGAAGCCGGAACGAATATCTGTCACCCATTCGGCGACTTTGGACTCGTTGGTGGCACGAACCACTACGGCAGACAGCATT
AGCCATTCGCTAGAACCTGGAAAGTGAAGCGGTTTTACCGCCTTAAGGCCGTCGTCGCCGGACTCATCAATGTAGGCGATGTAATCATAACGGGGTGTGG
ACATGGCAAAAGTGCAGACTCAAATCGAAAAATTCAAACAAGCCGCCCGCGAGCTCGAAACGGACAACGACGAAGATCGCTTCAATGAGAAGTTAGAGAA
GATCGCTCGGCGAAAACCCGGCGCAAATCCAAAATGGAGCGGGCAATTTCCGCCCGTTCTAGGCGGCCCTAACGAGGCTCCGGAGGATTAGCTTCCAGCC
ATTTGTTTGTGCCCGCCATCGCGTTGACGAACCAGCGCTCGCCACGGAATACTTTTGTGACCCATATGCGGTCATTGTGGTCAACAAAGTCTCGAAAATG
TTCGACTAGTTCCTGCGGGGTATTTGTGACGTTGATCAACCACGTCGAGAGCTGCGTTCGGTGTGCGCCGAGCCGCCGAAGTTCGTCCCACAGTCTTTGA
TAATCTATTTGTGCGTTCTTCTTCTCTTCGACCAAGTCATACGCGATCAAATAAACGCTCATCAGAATCTCCGGTTGAGGGATCAAGTTCCATCAACCTA
AGTGAATCGCGTAGGGTTTGCAAAGTGCATAATCGCC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
942 bp	313 aa	75	1016	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSVLSKKYFHDEAAAFRHLEKLLWGNGVICPKCGEVDRAGRLEGVRGKNGKARLGLWKCYGCRKQFTVRVGTVFESAHIPLHKCLQAVHLMVSSKKGVSS
HQLHRILEIQYKSAWFLAHRIREAMRDGKLAPMGGEGKIIEADETFTGRLAGQPKNKKGGWAHKNVVLTLVERGGIARSFHVDGTRVADIVPIVRANIRR
ESSLMTDEARHYISVGKEFASHDSVNHKEEEYVRGNVSTNTVEGYYSIFKRGMKGVYQHCSEKHLHRYLAEFDFRYSNRIALGVDDQDRADEAIRGMVGK
RLTYRVTDSAAAR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
870 bp	289 aa	2104	1235	-	No

ORF function : Passenger Gene
Annotation : Phage related proteinDescription :

ORF sequence :

MSTPRYDYIAYIDESGDDGLKAVKPLHFPGSSEWLMLSAVVVRATNESKVAEWVTDIRSGFRSKQAKALHFADLSHPNKVAVCRKISEMELRCFVVASNK
KNMEGYTNPDAAKIPSQCWFYCWMTRVLLERVTRFVLYRSMLDFGEPRYLRVEYSARGGLRYDQMHAYYEWLRMKRNKPFLPWGKLCFEVLHMNLLRVYP
HLEREGLQLADVVAASFFKACDKYDTSGCAPEYAKLLRPRMARESDKISGYGVKLLPSFSKAKLDSDQAEIFRYYGYPTQWWDPEAFSG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
189 bp	62 aa	2103	2291	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAKVQTQIEKFKQAARELETDNDEDRFNEKLEKIARRKPGANPKWSGQFPPVLGGPNEAPED

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
318 bp	105 aa	2586	2269	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MIPQPEILMSVYLIAYDLVEEKKNAQIDYQRLWDELRRLGAHRTQLSTWLINVTNTPQELVEHFRDFVDHNDRIWVTKVFRGERWFVNAMAGTNKWLEAN
PPEPR

Blast result :

Comments

ISNisp2 is 72% aa similar to ISRpa1.

References

1] Waterbury,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.