ISfinder Mirror Site

ISMno24

Family IS91
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001350	ND	Methylobacterium nodulans	Methylobacterium nodulans ORS 2060 plasmid pMNOD01

DNA section

IS Length : 2157 bp

Ends

oriIS : GGCGGTCGTGATCAGGCTCGGGGCCGCGGTCATGACGTGTTGATCCGAACGGCGCTTGGGCGCAGCTTCGGGACCTCGCCCCGGCGGAAGCGCTCGATGA II struct. : No
terIS : GGTCATGTCCTCGATCATCCGCCGGCGCAGCGGAGAGACGGCGTCGTGGGTCATGGGAGGCTCCCATCTGGGGTGAGGTGAACCTCACGATCCTCAGACA II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TCTGTCGACT		GGGCGACCGC

DNA sequence

GGCGGTCGTGATCAGGCTCGGGGCCGCGGTCATGACGTGTTGATCCGAACGGCGCTTGGGCGCAGCTTCGGGACCTCGCCCCGGCGGAAGCGCTCGATGA
CGACGAGGCGTCCGCCGCAGCACGGGCAGGCCGGTCCCGCGGGTGGGTCGGTCATGGCGGCGGGTTCGGGCTTGGCCGCGCACGGAGCCTGCATCGGCGC
CAGCAGGCTCCTGATCCGGGTGATGTTGGCAGCCCGCGTGCCGCTGGCGAACAGGCCGTAGTGGCGGATGCGGTGGAAGCCGTCAGGCAGCACGTGGAGC
AGGAAGCGGCGGATGAACTCGTCCGGGGCCAGCGTCATGGTCTTGATCCACTCGCGACCCTTCGCGCCATCCCGAGCCCGGTAGTCCTTCCAGCGGAACG
TGACGCCGCGCTCGTCCATAGCGATGAGCCGGCTGTTGGCGATGGCGACGCGGTGGGTGTAGCGCGACAGGTAGGCGAGCACGGCTTCCGGCCCAGCGAA
AGGGCGCTTGGCGTAGACGATCCACTCGGCGCGCCGCAGCGGTGCGAGATGAGCTGCAAAGGCGCCCGCCTCGGCCAGCGGGGCCAACTCGCCGTGGAAG
CCGAGCCTGCCGACGCGATGAGCCTCGGCCAGTCGGGTGAGGAAGAGCCGGCGGAACAGGCGTGAGAGCACCCGCACGGGCAGGAAGAAGCCTGGCCGGC
AGGCGATCCAGCGCGATCCGTCAGACGAGAGGCCGCCGCCCGGCACGACGATATGCACGTGCGGATGGTGCGTCATGGCCGAGCCCCAGGTGTGGAGCAC
GGCGGTGAGCCCGATCTTTGCGCCCAGATGCTTCGGATCGGCGGCGATGGTGAGGAGCGTGTCGGCGGCGGTGCGGAACAGCAGGTCGTATACTGCGGCT
TTGTTCTGGAAGGCCACGGCCGCGACCGGCGCCGGCAGGGTGAAGACGACGTGGTAGTAGGGCACGGGCAGCAGGTCGGCCTGACGCCGCTCGAGCCACG
CCCGCGCCGTTTGGCCCTGGCACTTCGGGCAGTGCCGGTTGCGGCACGAGTTGGAGGCGATGGCGACGTGGTCGCAGCCTTCGCAGCGCACGACATGACC
GCCGAGCGCCGCGGTGCGGCAGGCTCGGATCGCGGCCATGACCTTCATCTGGCCGTGGCTCGGGCGGTGACGCGCGAGGAAGGCGTCGCCATGCCGGTTC
AGGATATCCGCGACCTCAAGAGAAGACCGCGCCACGACGGGCGCGGCAGCCTACGGCGGTGGTGGGGATGCGAGCAGCGCGAGCGGGCTCGTGACCTCGC
GGATCGCCTTGAGCGCGACCCGGGTATAGACGGCGGTGGTGTCGAGCTTGCGGTGCCCGAGCAGCACCTGAATGACGCGGATGTCGGTCTTGCGCTCCAG
GAGGTGGGTGGCGAAGCTGTGCCGCAGCGTGTGCATCGAGACGCGCTTGTCGATCCGGGCGGCCTCGGCGGCGGCATGGCAGGCGCGGTTGAGCTGACGC
GTGGTGATCGGCTGGGCGGGCTGCTGGCCGGGGAAGAGCCAGCCGCGCGGGCGCTTCACCCGCCACCACTGCCGCAGCAGGTCGAGGAGGTCGGGCGAGA
GCATGACGAAGCGGTCCTTGCGGCCCTTGCCCTGCTCGACCCGGATCAGCATGCGCGCGCTATCGATGTCTGTGACCTTCAGGTTGGCGATCTCGGAGAC
GCGCAGGCCGCAGCCGTAGGCGACGCTCAACGCCGCGCGGTACTTCAGGCTCGGCGCGTGCGCGAGCAGGAGTGCCACCTCCTCGGGGCTGAGCACGACG
GGCAGCCGCTCGGGCGTGGGGATGCGGGCCAAGCGGTCGGCAAAGCCCGATCGGCCGAGCGTGACGTGGAAGAAGAACCGCAGCGCTGTGATGGCCAAGT
TCATGCGGGCATAGCTCGCGCCGAGCGAGGCCATGTGGAGCTGGTAGCGCCGGAGGTCCTCCGGCTCGGCCAGGTCCGGCGCGCGGCCGAGGAAGATCGT
GAACTCGCGGATCTGTCGGATGTAGTCGCGCTTTGTGTGCTCGCCGAACCGGCGGATGGTCATGTCCTCGATCATCCGCCGGCGCAGCGGAGAGACGGCG
TCGTGGGTCATGGGAGGCTCCCATCTGGGGTGAGGTGAACCTCACGATCCTCAGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1206 bp	401 aa	1235	30	-	No

ORF function : Transposase
Chemistry : Y2

ORF sequence :

MARSSLEVADILNRHGDAFLARHRPSHGQMKVMAAIRACRTAALGGHVVRCEGCDHVAIASNSCRNRHCPKCQGQTARAWLERRQADLLPVPYYHVVFTL
PAPVAAVAFQNKAAVYDLLFRTAADTLLTIAADPKHLGAKIGLTAVLHTWGSAMTHHPHVHIVVPGGGLSSDGSRWIACRPGFFLPVRVLSRLFRRLFLT
RLAEAHRVGRLGFHGELAPLAEAGAFAAHLAPLRRAEWIVYAKRPFAGPEAVLAYLSRYTHRVAIANSRLIAMDERGVTFRWKDYRARDGAKGREWIKTM
TLAPDEFIRRFLLHVLPDGFHRIRHYGLFASGTRAANITRIRSLLAPMQAPCAAKPEPAAMTDPPAGPACPCCGGRLVVIERFRRGEVPKLRPSAVRINT
S

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
861 bp	286 aa	2111	1251	-	No

ORF function : Accessory Gene
AG : IS91 integrase/resolvase

ORF sequence :

MTHDAVSPLRRRMIEDMTIRRFGEHTKRDYIRQIREFTIFLGRAPDLAEPEDLRRYQLHMASLGASYARMNLAITALRFFFHVTLGRSGFADRLARIPTP
ERLPVVLSPEEVALLLAHAPSLKYRAALSVAYGCGLRVSEIANLKVTDIDSARMLIRVEQGKGRKDRFVMLSPDLLDLLRQWWRVKRPRGWLFPGQQPAQ
PITTRQLNRACHAAAEAARIDKRVSMHTLRHSFATHLLERKTDIRVIQVLLGHRKLDTTAVYTRVALKAIREVTSPLALLASPPPP

Blast result :

Comments

ISMno24 is 80% (ORFA : the transposase) and 85% (ORFB : integrase) aa similar to ISMno23.

References

1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.