ISMma7

  • Family IS1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003901 ND Methanosarcina mazei
Methanosarcina mazei Go1
DNA section
IS Length : 740 bp

Ends


IR Length : 19/24

IRL : GGCTTTGGTAATTAAACATAGTTATCTATTATGAGAGCCTATAGTTCTGT
IRR : GGCATTGTTAATCAAATATAGCTAATTCTTTATTTCTGTGTTTCATCAAT

Insertion site


Left flankDirect repeatRight flankDR Length
CGGTAGGAAACAGATAATAATTCTCGATATATCCGGCTTTT9

DNA sequence

GGCTTTGGTAATTAAACATAGTTATCTATTATGAGAGCCTATAGTTCTGTATGGACTGCCCAAGATGTAATAGTTCCACTCACAAAAAGAACGGTATAGT
TGATGGACGTCAACGGTACAAATGCCATGATTGTGGATATAACTATTCCGTAGAGCTGAAATCAACTGCTAGCCCCACTTCTGTTAAGAGACAAGCTTTG
CAACTCTATCTTGAAGGATTAGGATTTCGCTCAATAGGGCGATTTTTAGGTGTAAGTCATGTTTCTGTTCAAAAATGGATAAAGAAATTTGGTCGGGAGC
TAGAGGACCTGAAAAGCGAAAATGAGATATCTATTGTTGAAATGGATGAGATGCACACTTACATCGGTAACAAAAAAAATATTGCTGGATATGGATTGCT
GTTGATAGAGTTGGGAAAAAATTCATCAACTGCTCTTTTGGCAGCAGAGGAACAGAAACTGGACAACTGATCTGGGAAAAATTAAAGACGAAAAAGATTG
AAGAAGTAATGACTGATCACTGGAAACCATATGCAGAGTTTATTCCAGAAACTATTCATACTCAATCAAAAGCAGAAACGTATACAGTTGAAGGATATAA
CAGCATATTCAGGCACTTTCTAGCAAGGTTGAGACGAAAGACAAAGTGTTATACGAAGAGTCTTGAAATGCTAAAGTACTCTGTTCTTCTATTGATGAAA
CACAGAAATAAAGAATTAGCTATATTTGATTAACAATGCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
420 bp139 aa51470+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDCPRCNSSTHKKNGIVDGRQRYKCHDCGYNYSVELKSTASPTSVKRQALQLYLEGLGFRSIGRFLGVSHVSVQKWIKKFGRELEDLKSENEISIVEMDE
MHTYIGNKKNIAGYGLLLIELGKNSSTALLAAEEQKLDN

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
363 bp120 aa371733+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

QKKYCWIWIAVDRVGKKFINCSFGSRGTETGQLIWEKLKTKKIEEVMTDHWKPYAEFIPETIHTQSKAETYTVEGYNSIFRHFLARLRRKTKCYTKSLEM
LKYSVLLLMKHRNKELAIFD

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
683 bp227 aa51733+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDCPRCNSSTHKKNGIVDGRQRYKCHDCGYNYSVELKSTASPTSVKRQALQLYLEGLGFRSIGRFLGVSHVSVQKWIKKFGRELEDLKSENEISIVEMDE
MHTYIGNKKNIAGYGLLLIELGKKFINCSFGSRGTETGQLIWEKLKTKKIEEVMTDHWKPYAEFIPETIHTQSKAETYTVEGYNSIFRHFLARLRRKTKC
YTKSLEMLKYSVLLLMKHRNKELAIFD

 

Blast result :
Comments
ISMma7 is 89% (ORF AB) aa similar.
There are one complete copy and one partial copy in Methanosarcina mazei Go1 genome.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baumer,S., Jacobi,C., Bruggemann,H., Lienard,T., Christmann,A., Bomeke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.P., Gunsalus,R.P., Fritz,H.J. and Gottschalk,G.(2002) J. Mol. Microbiol. Biotechnol. 4 (4), 453-461