ISMma14

  • Family IS66
  • Group ISBst12
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003901 ND Methanosarcina mazei
Methanosarcina mazei Go1
DNA section
IS Length : 1529 bp

Ends


IR Length : 22/30

IRL : GTAACTATTCAGGTAGCCTTAACGAGGCTACACATTTTTCCTCGTTCCGA
IRR : GTAACTGCTCAGAGTATAGTTAACGGCGCTCCTATGAGCGCCGCACGAAT

Insertion site


Left flankDirect repeatRight flankDR Length
AAAAGCTTTTGAATAATCTTGAATAAAA8
TAATATAATTAAAAAATTAAGAAATTCA8
ATAAATCAAAAAATTTAATTAAATAAAA8
GCTTTCTTTTCAATTCTTATTCATTTGT8
TTTTTCAGTTCTTTATTTTTTCCTTTTC8
AGTTTTAGTTTTTATTTTTATAATAACT8
ATAAATCTTTGTTAATTTTTCTAATTAA8
GTAAATACATAAATTTTACAGTAAAATA8
TAAAATAGTAAATTTTTCTTAAGTATTA8

DNA sequence

GTAACTATTCAGGTAGCCTTAACGAGGCTACACATTTTTCCTCGTTCCGAGGAAAAATTGGATATAAAATGATTCCTTTTTAGCTTTATGAAACGTGATG
AGATTCTTTCTTACTGTGCTTCAAATCCTGAAATTATTGTAGCTTACATTGAGAGTTTAGAATCTCAAGTTAAAGAACTCACTGAAAGACTTGTAGCCTT
AGAATCTCGTTTAAATCAAAACAGTCGTAACAGCAGTCGACCTCCTTCTACTGATTATTTTGTCAAAGAGAAACCTAATCCCAAGAGTCTGCGTAAACCA
AGCGGGAAGAAACCTGGAGGTCAGGAAGGTCATCCAGGCACGACTCTTGATATGGTTGATCATCCTGAGTAGGTAATAGAACATTCTTTGACCTGCTGCA
AAGAATGCGGATCTACCCTTGAGAATGTTGAAGTTGAAGCTTATGAGAGAAGACAGGTCTTTGACATTCCTCCCGTAAATCTAATTGTTACAGAACATAA
AAGTCAGATTAAAACCTGTCCTTGTTGTGGAAAGTTGAATAAAGCCGTTTTTCCCGAATCAGTGAAATATCCGGTTCAGTATGGCCCTAATATTTTAGCT
TCAGCTATTTACTGTAAAAATTACCAGTTTGTTCCTTATGATAGAATTTCTGAGTTATTTGAAGACATTATGGGAATAAAAATCTGTCCTGCTACGATAA
TTAGAGCAGAAAGAGAATGTTTCCAGAATTTAGAGGAATTTGAAAACGTTATTAGAGAGAAGTTATTAGCCTCGCCTGTAATCAATTTTGATGAAACTGG
TATGAAGATTGAAGGAAAAAGACACTGGCTTCATGTAGCTTCTAATGAAAAATACACATGTTATTTTGCTCATACAAAAAGAGGAGCAGAAGCAATAGAT
GCTATGGGAATTCTTCCGAAGTTTAAGGGAGTAGCAGTTCATGATGGATGGAAACCTTACAACGTTTATGATTGTGATCATGCTCTGTGTAACGCTCATT
TACAGAGAGAACTTACAGGAATTGAAGAGAACTATAAACAGACATGGGCTAAAGAGATGAATGAACTGCTCACTGAGATGAAGAAATACACTGATGAGTG
TAAAGAGCAATTAAGAGAACCTGATTTTGAGCAAATTAAAGCATTGGAAGAAAGGTTTGATGCAATTATCATTAGAGCGCTTGAAGAAAATCCGCATTCT
CTAAATCCTGAAAAACAGGGAAAACGCGGTAAAAATCCAAAAACAAAATCAAGGAATCTGCTGGATAGGTTTATAGAACACAAAGAAAAGATTCTGAGAT
TCCTGACAGATTTGAAAGTTCCATTTGATAATAATCAGGCAGAAAGAGATATCAGGATGATGAAACTACAGCAGAAAATATCGGGAACTTTCAGAAAAGC
AATGGGAGCGCAAGCTTTCTGCAGAATTAGGGCGTACATTTCTACAGGAAAAAAGAACGGTTTACCTGTTTTAGAGGGTATTCGTGCGGCGCTCATAGGA
GCGCCGTTAACTATACTCTGAGCAGTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1434 bp477 aa881521+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKRDEILSYCASNPEIIVAYIESLESQVKELTERLVALESRLNQNSRNSSRPPSTDYFVKEKPNPKSLRKPSGKKPGGQEGHPGTTLDMVDHPE*VIEHS
LTCCKECGSTLENVEVEAYERRQVFDIPPVNLIVTEHKSQIKTCPCCGKLNKAVFPESVKYPVQYGPNILASAIYCKNYQFVPYDRISELFEDIMGIKIC
PATIIRAERECFQNLEEFENVIREKLLASPVINFDETGMKIEGKRHWLHVASNEKYTCYFAHTKRGAEAIDAMGILPKFKGVAVHDGWKPYNVYDCDHAL
CNAHLQRELTGIEENYKQTWAKEMNELLTEMKKYTDECKEQLREPDFEQIKALEERFDAIIIRALEENPHSLNPEKQGKRGKNPKTKSRNLLDRFIEHKE
KILRFLTDLKVPFDNNQAERDIRMMKLQQKISGTFRKAMGAQAFCRIRAYISTGKKNGLPVLEGIRAALIGAPLTIL

 

Blast result :
Comments
ISMma14 is 94% aa similar to ISMma13.
There is a Stop codon at position 95 of the transposase.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baumer,S., Jacobi,C., Bruggemann,H., Lienard,T., Christmann,A., Bomeke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.P., Gunsalus,R.P., Fritz,H.J. and Gottschalk,G. (2002) J. Mol. Microbiol. Biotechnol. 4 (4), 453-461