ISMhu11

  • Family IS1
  • Group ISMhu11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007796 ND Methanospirillum hungatei
Methanospirillum hungatei JF-1
DNA section
IS Length : 1097 bp

Ends


IR Length : 15/23

IRL : GGTAGTGCCCTGTTAGCAAGCTGGTAATACTAAGTGCAAATAAAGTATTA
IRR : GGTACTGGCCTCCTTTAGAACTGAACATGGTAGTTTAAAAACTCACTCCA

Insertion site


Left flankDirect repeatRight flankDR Length
TTCATCAACCTTATACAGGTGAGAAAATTTCCTGATAAACAATATCAGTCAG8
TGATATTAGAACATAATAGAGTGGATTTTTTTCATAGAATCCATACATTAAT8
GAATTTTCCTCTTTGATCACTGTCCCTTCTCCACTGCTCACACAGACGGAGC8
CTATTTTCGGGTCACTTGCAACTAAAAATCCCATAAAAACGAAAAAGAAGGT8
TTTTCTTAATTAAATCATTGCTAATTACTCAATAGTTTAATAGTACTATTAT8
CTTACTCTATCGGTTACCCAATAAGCAATAGGACTTCCCGGAATCTTCTTAA8
CGAGAATTTCTAAGGTATCCAGCTTTCTTGCAACTGTATGACGATTAATCCC8
CTCACCGGAGATATGACATGGAAAAACTTCCGTGTGAGACAATAGTGTGGGA8
CTTCTGGTCCACATTTTCATTGAAAATTTTGCGGTGATATTAAAACCGATAG8

DNA sequence

GGTAGTGCCCTGTTAGCAAGCTGGTAATACTAAGTGCAAATAAAGTATTAAATTTTATGTACTAAAAATTAATATAAGTTTAGATATAATTATTTTCATG
GTTGGAAAACGTGGTCCTATATTGATTACGTGTCAAAATCCGGATTGTACTTATTTTCAAATTGAAGATGGAAAGAATATTACTAAAAATGGGCATAATT
CTGCAGGCAATCAACAGTACTATTGCCATCACTGTCGACGTTTTTTTATTGAAACTAAAAATACTCCTCTGTATGATTCTCGTTTACCACGAACTGCTGT
TCTCATAATTGCGAAACACTCGACTGAAAAAACTTCAATTCGAGGAGTATCTCGAGTTACCGGACACCATCGCGACACTATATCTCGATATTATCACTTA
ATCGGTGAACATGCTGAAAAACTCAACGATTATTTTATTCATGACATTTCCGCGGGGGATTGTGAGATGGATGAAATTTGGGAGTTTGTTCAAAAAAAAA
CAAGAATCTCCTAACAACTGACTCTGAAGAATTAGGTGATTGTTGGTCCTATACTTGTTTTAAAAGGGATTCTGGATTATTTTTAGCATTTGAATCTGGA
AAAAGAAATATTGATACTTGTGCAGATATGTTAGTTCGATTTTTTAACCGAATGGAACTTCCAACGCCAGAAAACAAAATATCGATTTTTACTGATGGAA
ACGTCCAATATAGCATTTGTTTACCTGAATTATATTGTGAGCCATGCTTGGATTATGGTCAGGTGATCAAAGTAAAAGAGAAGAATAAACTTGTTTATGT
CATTCGAGAGAAAATTATGGGAAATCCAGATAGTAAAGCAATCTCTACCTCAGTCATTGAGGGCTACAATAATAAAATCAGACAACGATTGAGCCGTTTT
GGAAGGAAAACTGCCTCTTTTTCAAAAAAACTCAATAGATTCATTTCAGCGCTAAATATCTTTCAATTTGTGCATAATTTTATCGAAGTAAAAAGTAGTC
ACAAGTCTCCAGCAATGTTAGAAAGTATTACTGATCATCTTTGGAATTGGAGTGAGTTTTTAAACTACCATGTTCAGTTCTAAAGGAGGCCAGTACC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
417 bp138 aa98514+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MVGKRGPILITCQNPDCTYFQIEDGKNITKNGHNSAGNQQYYCHHCRRFFIETKNTPLYDSRLPRTAVLIIAKHSTEKTSIRGVSRVTGHHRDTISRYYH
LIGEHAEKLNDYFIHDISAGDCEMDEIWEFVQKKTRIS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
609 bp202 aa4751083+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

NLGVCSKKNKNLLTTDSEELGDCWSYTCFKRDSGLFLAFESGKRNIDTCADMLVRFFNRMELPTPENKISIFTDGNVQYSICLPELYCEPCLDYGQVIKV
KEKNKLVYVIREKIMGNPDSKAISTSVIEGYNNKIRQRLSRFGRKTASFSKKLNRFISALNIFQFVHNFIEVKSSHKSPAMLESITDHLWNWSEFLNYHV
QF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
986 bp328 aa981083+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVGKRGPILITCQNPDCTYFQIEDGKNITKNGHNSAGNQQYYCHHCRRFFIETKNTPLYDSRLPRTAVLIIAKHSTEKTSIRGVSRVTGHHRDTISRYYH
LIGEHAEKLNDYFIHDISAGDCEMDEIWEFVQKKNKNLLTTDSEELGDCWSYTCFKRDSGLFLAFESGKRNIDTCADMLVRFFNRMELPTPENKISIFTD
GNVQYSICLPELYCEPCLDYGQVIKVKEKNKLVYVIREKIMGNPDSKAISTSVIEGYNNKIRQRLSRFGRKTASFSKKLNRFISALNIFQFVHNFIEVKS
SHKSPAMLESITDHLWNWSEFLNYHVQF

 

Blast result :
Comments
ISMhu11 is 39% aa similar to ISLysp1.
The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Kiss,H., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N., McInerney,M.J., Brockman,F., Culley,D., Ferry,J.G., Gunsalus,R.P., Morrison,M., Plugge,C., Scholten,J., Stams,A.J.M., Boone,D.R. and Richardson,P. (2006) Direct submission GenBank.