ISLre2
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP007281 | Y | Lactobacillus reuteri | Lactobacillus reuteri 100-23 Lactobacillus reuteri JCM 1112 Lactobacillus reuteri DSM 20016 Lactobacillus reuteri SD2112 Lactobacillus johnsonii DPC 6026 |
DNA section
IS Length : 1570 bp
Ends
IR Length : 18/25
IRL : GTTAGTGTAAGATTTTCATAGGTTGGATGAATAATTCATCTGGTCGTGGA
IRR : GTATCTGTCAAGTTTTCATAGGTAGCCTTTAAATATACAGTTTTAGTGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CACCCACTTA | GGAGTCCTA | TTCCTACTTC | 9 |
| AAAATGTAAG | GGCAACCAT | TCTGCTGTTG | 9 |
| TTGATTCTAG | TCTCCTATT | TCTCCTATTT | 9 |
| TTGATTCTAG | TCTCCTATT | TTAGTATTGG | 9 |
| CACCCACTTA | GGAGTCCTA | TTCCTACTTC | 9 |
| AAAATGTAAG | GGCAACCAT | GGCAACCATT | 9 |
DNA sequence
GTTAGTGTAAGATTTTCATAGGTTGGATGAATAATTCATCTGGTCGTGGAAATCTTTTTTGTAGACCAATTTACTTATTTACAAAAAAAGAAAAGACCAG
TAGCCTTTAGTGTGTCGAATACTAAGCAAAGGCAGGTCTTTCCTCATGGATATTTTAACAGAAATCGAGCAGAATTTGGTGAAATCAGGCAGTTTATTTG
AAGCTGAACAGATTATTTTAAAAGGGGTATTGGAGTTAGGACAAGTAATCATGCAAAACTTTTTGGAAAGCTTAGATCGAAGCTTAAAGTCCCAAGCTCC
AGCGAACTATCAAGTAATCAATAAACAGCCACGGACGCTTAATTTTATCTTTGGCCCGGTGACTTTTCAACGACGGTATTATCAGGCTGGGACAAAGAAA
CGTGAATTTTACTTAGACCAACAATTAAAAATTAAACCACGTCGTCGTTTATCGCCACACTACTTAATGATGATGGCTAAGATTGCCCAAACAACTACAA
TGCGCAATACTGCCGACATTTTGAACCTTGTATTTGACAGCGGAATTACTGCCGATTCGGTAATGCACGCCGTGCATGAGTTAGGAAATCAGGTAGCTAA
ACAAACTCAAGCAAAAGAACACCAAGCTACTCCTCGCCATATGCCTAAAAATTTAACTATTGAGGGTGATGCCTTTATGATTAAAGGTAAAAAAGAAGCA
GGTCAGCTGACTCTTGTGCACCATTATCGGGTTTATGAGCGAGTAGCTAATCAAATCATTAATCGGCATGACTTTCTCAGTGTTGGGCACCAAGGACGGC
TTGAAGCACGACTAAGTGATTATTTAGACCGCCATTATAAGCTTGCCGGTCAAACGATCTTTTTGGCCAGTGACGCTGGCCCAGGTTACGAACCAGCTAA
GCTATTAAGTCTAGTTCCTCAAGGTGCACATGGTGAATACTTTCTCGACCGCTATCATTGTTTACAGAAAATTGAACATACTTTAGGCCGGCACAACGAA
TTAGCCATGCGAGCAATTAAAGCCGTTCGTCATCATGATCAAGCAGAGCTAACAATAATTTTAGATACTTATGAATCACAAAACCTAACGGAAAAACAAG
CAGACGACCTAATGCGTTTAAGAAAGTATCTACAGCGAAATTGGCGGTATATCCTCTCACCACAAATGCGTGGATTTAAGGATATTCATTTAATTGGTTC
AGTCGAAAGTTCTCACCGGGCTTTTACTTACCGGATGAAGAAACAGGGCAAGTCATGGACTAAGCAGGGGGCTAAAGCCATGATTGGTTTAATTGAAGCC
CGAATGAATGGTGAACTGCAAGCTAGTTTAAATACAATCCTAGAACAATTAACAGTTCTTCCTCGAGTGGCTCAAACCAGCCTATTACAGGAAATGCATA
TTCGAACTGGAGAGTTTCTAAGAAAGGCACCGACAAAGCCGTCAATTGGAGTAGTACAAGGAATAATTCCGATTAATACGGCCACAAGTAGACCAATAGG
ACAACTTTTTAAGGCACTAACCCACTAAAACTGTATATTTAAAGGCTACCTATGAAAACTTGACAGATAC
TAGCCTTTAGTGTGTCGAATACTAAGCAAAGGCAGGTCTTTCCTCATGGATATTTTAACAGAAATCGAGCAGAATTTGGTGAAATCAGGCAGTTTATTTG
AAGCTGAACAGATTATTTTAAAAGGGGTATTGGAGTTAGGACAAGTAATCATGCAAAACTTTTTGGAAAGCTTAGATCGAAGCTTAAAGTCCCAAGCTCC
AGCGAACTATCAAGTAATCAATAAACAGCCACGGACGCTTAATTTTATCTTTGGCCCGGTGACTTTTCAACGACGGTATTATCAGGCTGGGACAAAGAAA
CGTGAATTTTACTTAGACCAACAATTAAAAATTAAACCACGTCGTCGTTTATCGCCACACTACTTAATGATGATGGCTAAGATTGCCCAAACAACTACAA
TGCGCAATACTGCCGACATTTTGAACCTTGTATTTGACAGCGGAATTACTGCCGATTCGGTAATGCACGCCGTGCATGAGTTAGGAAATCAGGTAGCTAA
ACAAACTCAAGCAAAAGAACACCAAGCTACTCCTCGCCATATGCCTAAAAATTTAACTATTGAGGGTGATGCCTTTATGATTAAAGGTAAAAAAGAAGCA
GGTCAGCTGACTCTTGTGCACCATTATCGGGTTTATGAGCGAGTAGCTAATCAAATCATTAATCGGCATGACTTTCTCAGTGTTGGGCACCAAGGACGGC
TTGAAGCACGACTAAGTGATTATTTAGACCGCCATTATAAGCTTGCCGGTCAAACGATCTTTTTGGCCAGTGACGCTGGCCCAGGTTACGAACCAGCTAA
GCTATTAAGTCTAGTTCCTCAAGGTGCACATGGTGAATACTTTCTCGACCGCTATCATTGTTTACAGAAAATTGAACATACTTTAGGCCGGCACAACGAA
TTAGCCATGCGAGCAATTAAAGCCGTTCGTCATCATGATCAAGCAGAGCTAACAATAATTTTAGATACTTATGAATCACAAAACCTAACGGAAAAACAAG
CAGACGACCTAATGCGTTTAAGAAAGTATCTACAGCGAAATTGGCGGTATATCCTCTCACCACAAATGCGTGGATTTAAGGATATTCATTTAATTGGTTC
AGTCGAAAGTTCTCACCGGGCTTTTACTTACCGGATGAAGAAACAGGGCAAGTCATGGACTAAGCAGGGGGCTAAAGCCATGATTGGTTTAATTGAAGCC
CGAATGAATGGTGAACTGCAAGCTAGTTTAAATACAATCCTAGAACAATTAACAGTTCTTCCTCGAGTGGCTCAAACCAGCCTATTACAGGAAATGCATA
TTCGAACTGGAGAGTTTCTAAGAAAGGCACCGACAAAGCCGTCAATTGGAGTAGTACAAGGAATAATTCCGATTAATACGGCCACAAGTAGACCAATAGG
ACAACTTTTTAAGGCACTAACCCACTAAAACTGTATATTTAAAGGCTACCTATGAAAACTTGACAGATAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1383 bp | 460 aa | 146 | 1528 | + | No |
Chemistry : DDE
ORF sequence :
MDILTEIEQNLVKSGSLFEAEQIILKGVLELGQVIMQNFLESLDRSLKSQAPANYQVINKQPRTLNFIFGPVTFQRRYYQAGTKKREFYLDQQLKIKPRR
RLSPHYLMMMAKIAQTTTMRNTADILNLVFDSGITADSVMHAVHELGNQVAKQTQAKEHQATPRHMPKNLTIEGDAFMIKGKKEAGQLTLVHHYRVYERV
ANQIINRHDFLSVGHQGRLEARLSDYLDRHYKLAGQTIFLASDAGPGYEPAKLLSLVPQGAHGEYFLDRYHCLQKIEHTLGRHNELAMRAIKAVRHHDQA
ELTIILDTYESQNLTEKQADDLMRLRKYLQRNWRYILSPQMRGFKDIHLIGSVESSHRAFTYRMKKQGKSWTKQGAKAMIGLIEARMNGELQASLNTILE
QLTVLPRVAQTSLLQEMHIRTGEFLRKAPTKPSIGVVQGIIPINTATSRPIGQLFKALTH
RLSPHYLMMMAKIAQTTTMRNTADILNLVFDSGITADSVMHAVHELGNQVAKQTQAKEHQATPRHMPKNLTIEGDAFMIKGKKEAGQLTLVHHYRVYERV
ANQIINRHDFLSVGHQGRLEARLSDYLDRHYKLAGQTIFLASDAGPGYEPAKLLSLVPQGAHGEYFLDRYHCLQKIEHTLGRHNELAMRAIKAVRHHDQA
ELTIILDTYESQNLTEKQADDLMRLRKYLQRNWRYILSPQMRGFKDIHLIGSVESSHRAFTYRMKKQGKSWTKQGAKAMIGLIEARMNGELQASLNTILE
QLTVLPRVAQTSLLQEMHIRTGEFLRKAPTKPSIGVVQGIIPINTATSRPIGQLFKALTH
Blast result :
Comments
The transposase amino acid sequence is 29% similar to the transposase of the integrative and conjugative element (ICE) TnGBS1 of Streptococcus agalactiae NEM316 [1].
As for TnGBS element, ISLre2 is inserted 15 bp upstream the -35 box of typical sigmaA promoters. An extrachromosomal circular form of the IS have been amplified by PCR.
As for TnGBS element, ISLre2 is inserted 15 bp upstream the -35 box of typical sigmaA promoters. An extrachromosomal circular form of the IS have been amplified by PCR.
References