ISH8
- Family IS4
- Group ISH8
Isoform ISH26Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M58557 [P] | Y | Halobacterium salinarum | Halobacterium salinarum SD108 Halobacterium salinarum NRC-1 plasmid pNRC100 |
DNA section
IS Length : 1402 bp
Ends
IR Length : 16/18
IRL : CATCCGTCTTTAGTTAAGCGAGAAACCGCGTGATAGCGGCGGCTTATCGA
IRR : CATTCGTCTTCAGTTAAGACTCACACCTCGGTTGTGTAGCGGTAGCGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | GACGGGTGGC | TTGATNNNNN | 10 |
| CGCTTGCAGC | CACTCCACGG | TTGACGAGCC | 10 |
| GAACCATTTC | AGGTATAGTA | CTAGCCCGGC | 10 |
| TCTCCGCCTC | CCGCGCGAGC | TTCGCCTCGG | 10 |
| GTCCCTTTTT | ACTCTTCCCC | AGCTCCCGGT | 10 |
| TGTGGACAAT | ATCATTGACT | TGCTCTGTAT | 10 |
| TGTGTCACAG | GGGACAAAGC | TTATTCTACG | 10 |
| TGTGTCACAG | GGGACAAAGC | TTATTCTACA | 10 |
| ATGAGGACAT | GTTGTAGACGT | AGCCGTCGAT | 11 |
| AGGGGTATCC | TCACGCCAGG | TAGATCCCA | 10 |
| GGTTGAATAA | TGCGTACAGG | ACTTCGGCC | 10 |
| TGTGTCACAG | GGGACAAAGC | TTATTCTACA | 10 |
DNA sequence
CATCCGTCTTTAGTTAAGCGAGAAACCGCGTGATAGCGGCGGCTTATCGAGGCACCTTTCGGAAGGAATGAGGAAGTCCAGTCTGTGCACGACAAGGACT
CCTCATCTCGGATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTTGAAGAGCACGCCGAGGAACTCGGCGTGGTCGAACGTGACCGCAAGCT
CCAGATCCCTGCCTTCGTTTGGGCATTCGTGTTCGGCTTCGCCGCAGGCGAAAGCCGAACACTCGCCGGGTTTAGACGCTGTTACAACTCGACTGCCGAT
GAGACGATCTCTCCGGGTGGATTCTATCAGCGGTTGACACCGACGCTTGCGGAGTATCTCCGCGACCTCGTTGAGCATGGTCTCGACGAGGTCGCTGTTC
CTAACGCTGTTGACGCTGATATCGACCGATTTAGAGACGTGATGATCGCTGATGGAACGGTGTTGCGGTTACACGAATTTCTCTCAGATCAGTTCGAAGC
CCGCCACGAGGAGCAGGCTGGAGCGAAGCTCCACCTGCTCCACAATGCCACAGAGCAGACGATTGAACGGCTCGATACTGCTAACGAGAAAACGCACGAC
AGCACCTTGTTCAAAACAGGGCCGTGGCTTGAGAATCGCCTCGTGTTGTTCGATCTCGCCTACTTCAAGTACCGCCGGTTTGCGCTGATCGACGAAAACG
ACGGCTACTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGCTGATTACGAGGGAGTTACGGGAATGGCGCGGTCGCGCCATTCCCTTAGAGGGCAAGCA
GCTCCGAGCTGTTCTCGACGATCTCGATCGGAAGTACATCGATGTAGAAGTCGAAGTCGAATTCAAACGAGGGCCGTACAATGGGACGCGGTCACTAGAT
ACGAAGCGATTTCGCGTCGTCGGCGTCCGCGACGAAGACGCCGACGACTACCATCTGTACATGACGAATTTGGTGAGAAAAGAGTTCTTCCCGGCTGATT
TAGCACAGATCTACCGATGTCGGTGGGAAGTTGAGTTGTTGTTCCGTGAGCTGAAGACGCAGTACAACTTGGATGAGTTCAACACGAGCGACGAACACGT
GGTGAAGATCTTACTATACGCAGCACTGCTGTCGCTGCTTGTAAGCCGCGATTTATTGGATCTGGTCACTGAGCAGGCGGATGATGAGCTCGTGTTTCCG
ACAGAACGCTGGGCGGCGACCTTCCGGTCGCACGCCCAGCTTTTCCTCCACAAACTGGGTGAGTATCTCGGCTACTCACCACCGCCGCTGCTTGAACGAC
TGACTGAAGACGCTCAAAAGATCCACAAGCAGCGACCGATCTTACAAGAGACGCTCGCTACCGCTACACAACCGAGGTGTGAGTCTTAACTGAAGACGAA
TG
CCTCATCTCGGATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTTGAAGAGCACGCCGAGGAACTCGGCGTGGTCGAACGTGACCGCAAGCT
CCAGATCCCTGCCTTCGTTTGGGCATTCGTGTTCGGCTTCGCCGCAGGCGAAAGCCGAACACTCGCCGGGTTTAGACGCTGTTACAACTCGACTGCCGAT
GAGACGATCTCTCCGGGTGGATTCTATCAGCGGTTGACACCGACGCTTGCGGAGTATCTCCGCGACCTCGTTGAGCATGGTCTCGACGAGGTCGCTGTTC
CTAACGCTGTTGACGCTGATATCGACCGATTTAGAGACGTGATGATCGCTGATGGAACGGTGTTGCGGTTACACGAATTTCTCTCAGATCAGTTCGAAGC
CCGCCACGAGGAGCAGGCTGGAGCGAAGCTCCACCTGCTCCACAATGCCACAGAGCAGACGATTGAACGGCTCGATACTGCTAACGAGAAAACGCACGAC
AGCACCTTGTTCAAAACAGGGCCGTGGCTTGAGAATCGCCTCGTGTTGTTCGATCTCGCCTACTTCAAGTACCGCCGGTTTGCGCTGATCGACGAAAACG
ACGGCTACTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGCTGATTACGAGGGAGTTACGGGAATGGCGCGGTCGCGCCATTCCCTTAGAGGGCAAGCA
GCTCCGAGCTGTTCTCGACGATCTCGATCGGAAGTACATCGATGTAGAAGTCGAAGTCGAATTCAAACGAGGGCCGTACAATGGGACGCGGTCACTAGAT
ACGAAGCGATTTCGCGTCGTCGGCGTCCGCGACGAAGACGCCGACGACTACCATCTGTACATGACGAATTTGGTGAGAAAAGAGTTCTTCCCGGCTGATT
TAGCACAGATCTACCGATGTCGGTGGGAAGTTGAGTTGTTGTTCCGTGAGCTGAAGACGCAGTACAACTTGGATGAGTTCAACACGAGCGACGAACACGT
GGTGAAGATCTTACTATACGCAGCACTGCTGTCGCTGCTTGTAAGCCGCGATTTATTGGATCTGGTCACTGAGCAGGCGGATGATGAGCTCGTGTTTCCG
ACAGAACGCTGGGCGGCGACCTTCCGGTCGCACGCCCAGCTTTTCCTCCACAAACTGGGTGAGTATCTCGGCTACTCACCACCGCCGCTGCTTGAACGAC
TGACTGAAGACGCTCAAAAGATCCACAAGCAGCGACCGATCTTACAAGAGACGCTCGCTACCGCTACACAACCGAGGTGTGAGTCTTAACTGAAGACGAA
TG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1275 bp | 424 aa | 115 | 1389 | + | No |
Chemistry : DDE
ORF sequence :
MRRLTTLFPSEFLEEHAEELGVVERDRKLQIPAFVWAFVFGFAAGESRTLAGFRRCYNSTADETISPGGFYQRLTPTLAEYLRDLVEHGLDEVAVPNAVD
ADIDRFRDVMIADGTVLRLHEFLSDQFEARHEEQAGAKLHLLHNATEQTIERLDTANEKTHDSTLFKTGPWLENRLVLFDLAYFKYRRFALIDENDGYFV
SRLKQNANPLITRELREWRGRAIPLEGKQLRAVLDDLDRKYIDVEVEVEFKRGPYNGTRSLDTKRFRVVGVRDEDADDYHLYMTNLVRKEFFPADLAQIY
RCRWEVELLFRELKTQYNLDEFNTSDEHVVKILLYAALLSLLVSRDLLDLVTEQADDELVFPTERWAATFRSHAQLFLHKLGEYLGYSPPPLLERLTEDA
QKIHKQRPILQETLATATQPRCES
ADIDRFRDVMIADGTVLRLHEFLSDQFEARHEEQAGAKLHLLHNATEQTIERLDTANEKTHDSTLFKTGPWLENRLVLFDLAYFKYRRFALIDENDGYFV
SRLKQNANPLITRELREWRGRAIPLEGKQLRAVLDDLDRKYIDVEVEVEFKRGPYNGTRSLDTKRFRVVGVRDEDADDYHLYMTNLVRKEFFPADLAQIY
RCRWEVELLFRELKTQYNLDEFNTSDEHVVKILLYAALLSLLVSRDLLDLVTEQADDELVFPTERWAATFRSHAQLFLHKLGEYLGYSPPPLLERLTEDA
QKIHKQRPILQETLATATQPRCES
Blast result :
Comments
ISH8 has been isolated through its inactivation of the gvp operon (gas vesicle proteins, Vac-) (Halladay et al., 1993). ISH8 DNA sequence is 94 % identical to that of ISH26. No full-length putative transposase is apparently present inside ISH8 (but its DNA sequence needs to be confirmed; DasSarma, pers. comm.). However, there is a 28 % identity between a long ISH8 ORF and the putative IS5377 transposase, over a 233-aa overlap.
Copies of ISH8 were present in Halobacterium salinarum pNRC100 (see ISH8A, ISH8B, ISH8C, ISH8D, ISH8E).
Copies of ISH8 were present in Halobacterium salinarum pNRC100 (see ISH8A, ISH8B, ISH8C, ISH8D, ISH8E).
References
1] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.
2] Ng, W.-L., Kothakota, S., and DasSarma, S. (1991) J. Bacteriol. 173, 1958-1964.
3] Halladay, J.T., Donovan, J.W., Jones, J.G., Young, D.C., and DasSarma, S. (1993) Unpublised results.
2] Ng, W.-L., Kothakota, S., and DasSarma, S. (1991) J. Bacteriol. 173, 1958-1964.
3] Halladay, J.T., Donovan, J.W., Jones, J.G., Young, D.C., and DasSarma, S. (1993) Unpublised results.