ISH1
- Family IS5
- Group ISH1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| J01725 | Y | Halobacterium salinarum | Halobacterium tunesiensis A2 Halobacterium salinarum R1 Halobacterium salinarum NRC34020 Halobacterium salinarum NRL Halobacterium salinarum S9 Halobacterium salinarum NRC-1 Halobacterium salinarum pHS1 Halobacterium salinarum R1mR Halobacterium salinarum YC81819-9 |
DNA section
IS Length : 1118 bp
Ends
IR Length : 8/9
IRL : TGCCTTGTTTTGCCACCGATTGAGGGAAGTTTCAGACTCTCTCCCGGGAA
IRR : TGCCTCGTTGAATCACAAGACAGCGTCGGGGTAGGGAAGACTATTCGCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCATGTTGG | AGTTATTG | CCAACAGCAG | 8 |
| NNNNTGTTGG | CAATAACT | CCAACANNNN | 8 |
| NNNNTGTTGG | CGGCGTGT | TCTACGNNNN | 8 |
| TCTCGGTTGA | AGAGATCA | TCGACCGCGT | 8 |
| AGGAAGTTGG | TGATGAGT | TCGGAGCCGA | 8 |
| GTATGCTTGA | ACCCGTAG | TGTACTTGAA | 8 |
| TGGAAGTCGA | AGTCGAGT | TCAAGCGCGG | 8 |
DNA sequence
TGCCTTGTTTTGCCACCGATTGAGGGAAGTTTCAGACTCTCTCCCGGGAAGATTCCGTCAAGCTAACCAGGAATTGGACGCCGTCTGGCGATATGGCATC
GCTCAGACGGCTTGCTTGGATGTGTCGAAACCTTGCCAAACAGCACGTTGACGATCCGGACGTACCCGCCGCGCCGTCCGGCGCGGGCGGGTACGCCGAG
TGGGTGCAGATCGCGTTGATTCTGTACCGTGTCGAACTGGAAAAGAGCCTCCGTGAATCCGAGGACTACCTCAACGAGATGCCCGGTGTTCTTGCCGTGT
TTGGACTTGACGAAGCACCACACTACAGCTCGTTCTGCCGGTGGGAAAACGAGTATCGAATGCGTGAGCTCCGCCGCCTGCTCCGCGCTTCGGCGGAGCA
GGCGGGCTGGAGTGGCGAAGCCGCGATTGACGCGAGCGGCTTCCAGCGCGATCAAACCAGCTACCACTACCGCGACCGCGCGAATTACTCGTTCCAGTCG
ATGAAGACGACGATCTTGATCGACGTGAACTCGCTAGCGATCAAGGACGTTCACTACACGACGCAGAAGCCTGGGACGGCCACATTGGGATGCAGGTCTT
CCGCCGGAAACGCGGAAGACCTGCGGGTGCTGTCTGCTGACGCGAACTACTCGTGGAGCGACCTCCGTGAGGAGTGTCGCTCCGAATCAACGCGACCGTT
GATCAAGCACAGGGAGCAAACACCGTTGCAGAAGGCTCACCACGCCCGGATGAACGAGGACTACAACCAACGCTGGATGAGTGAAACCGGCTTCTCGCAG
TTGAAGGAAGACGACGGCGAGAAGCTGCGCTCCGGAGCTGGCAGGCCAGTTCCGGAGCTGACTCGGAAGTGCATCATCCATAACCTGACGCAGGCGGCGA
GTTAAGGGCTCGCCGCCTGCTCGCTTTCTCCGTACGTATCCGGAGAGGCATCGCCGTCGTCATCGGAACAACGAAGCAAGATACCATAGTTGTGACCCTT
CAGCAACCGCCGTGAGTGACAGCTACTGCATCTTCTGAGGTCAAGAACCCGTCTCTGACGCTGTGAAACTGCGAATAGTCTTCCCTACCCCGACGCTGTC
TTGTGATTCAACGAGGCA
GCTCAGACGGCTTGCTTGGATGTGTCGAAACCTTGCCAAACAGCACGTTGACGATCCGGACGTACCCGCCGCGCCGTCCGGCGCGGGCGGGTACGCCGAG
TGGGTGCAGATCGCGTTGATTCTGTACCGTGTCGAACTGGAAAAGAGCCTCCGTGAATCCGAGGACTACCTCAACGAGATGCCCGGTGTTCTTGCCGTGT
TTGGACTTGACGAAGCACCACACTACAGCTCGTTCTGCCGGTGGGAAAACGAGTATCGAATGCGTGAGCTCCGCCGCCTGCTCCGCGCTTCGGCGGAGCA
GGCGGGCTGGAGTGGCGAAGCCGCGATTGACGCGAGCGGCTTCCAGCGCGATCAAACCAGCTACCACTACCGCGACCGCGCGAATTACTCGTTCCAGTCG
ATGAAGACGACGATCTTGATCGACGTGAACTCGCTAGCGATCAAGGACGTTCACTACACGACGCAGAAGCCTGGGACGGCCACATTGGGATGCAGGTCTT
CCGCCGGAAACGCGGAAGACCTGCGGGTGCTGTCTGCTGACGCGAACTACTCGTGGAGCGACCTCCGTGAGGAGTGTCGCTCCGAATCAACGCGACCGTT
GATCAAGCACAGGGAGCAAACACCGTTGCAGAAGGCTCACCACGCCCGGATGAACGAGGACTACAACCAACGCTGGATGAGTGAAACCGGCTTCTCGCAG
TTGAAGGAAGACGACGGCGAGAAGCTGCGCTCCGGAGCTGGCAGGCCAGTTCCGGAGCTGACTCGGAAGTGCATCATCCATAACCTGACGCAGGCGGCGA
GTTAAGGGCTCGCCGCCTGCTCGCTTTCTCCGTACGTATCCGGAGAGGCATCGCCGTCGTCATCGGAACAACGAAGCAAGATACCATAGTTGTGACCCTT
CAGCAACCGCCGTGAGTGACAGCTACTGCATCTTCTGAGGTCAAGAACCCGTCTCTGACGCTGTGAAACTGCGAATAGTCTTCCCTACCCCGACGCTGTC
TTGTGATTCAACGAGGCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 813 bp | 270 aa | 93 | 905 | + | No |
Chemistry : DDE
ORF sequence :
MASLRRLAWMCRNLAKQHVDDPDVPAAPSGAGGYAEWVQIALILYRVELEKSLRESEDYLNEMPGVLAVFGLDEAPHYSSFCRWENEYRMRELRRLLRAS
AEQAGWSGEAAIDASGFQRDQTSYHYRDRANYSFQSMKTTILIDVNSLAIKDVHYTTQKPGTATLGCRSSAGNAEDLRVLSADANYSWSDLREECRSEST
RPLIKHREQTPLQKAHHARMNEDYNQRWMSETGFSQLKEDDGEKLRSGAGRPVPELTRKCIIHNLTQAAS
AEQAGWSGEAAIDASGFQRDQTSYHYRDRANYSFQSMKTTILIDVNSLAIKDVHYTTQKPGTATLGCRSSAGNAEDLRVLSADANYSWSDLREECRSEST
RPLIKHREQTPLQKAHHARMNEDYNQRWMSETGFSQLKEDDGEKLRSGAGRPVPELTRKCIIHNLTQAAS
Blast result :
Comments
The bacterio-opsin (BO) gene codes for the purple membrane protein. Eleven spontaneous mutant strains of Halobacterium salinarum S9 (formerly H. halobium) have been isolated which are deficient in the purple membrane protein (Pum-). In the Pum- mutants, the bacterio-opsin gene has been inactivated by the insertion of one of two transposable elements (ISH1 or ISH2) into the BO gene. ISH1 is a site-specific transposable element (isolated 21 times at the same site, in both orientations) inserting into an 8 bp target sequence 5'-AGTTATTG-3'. The number of ISH1 copies in Halobacterium salinarum ranges from one to more than five in different strains. In at least one strain, ISH1 occurs on both the chromosomal and plasmid DNA.
In Halobacterium salinarum R1, ISH1 carries ponctual deletion/insertion which induce non-programmed frameshifts on 2 parts of the transposase. The protein does not match at positions : AA 160 to AA 172, and AA 248 to AA 254.
File updated: June 03 2010.
In Halobacterium salinarum R1, ISH1 carries ponctual deletion/insertion which induce non-programmed frameshifts on 2 parts of the transposase. The protein does not match at positions : AA 160 to AA 172, and AA 248 to AA 254.
File updated: June 03 2010.
References
1] Pfeifer F., Weidinger G., and Goebel W. (1981) J. Bacteriol. 145, 375-381.
2] Simsek M., DasSarma S., RajBhandary U.L., and Khorana H.G. (1982) Proc. Natl. Acad. Sci., USA 79, 7268-7272.
3] Pfeifer F., Betlach M., Martienssen R., Friedman J., and Boyer H.W. (1983) Mol. Gen. Genet. 191, 182-188.
4] DasSarma S., RajBhandary U.L., and Khorana H.G. (1983) Proc. Natl. Acad. Sci., USA 80, 2201-2205.
5] DasSarma S., RajBhandary U.L., and Khorana H.G. (1984) Proc. Natl. Acad. Sci., USA 81, 125-129.
6] Simsek M. (1985) in Alaeddinoglun, G., Demain, A.L., and Lancini, G. (Eds) Plenum Press, N.Y. pp 9-17.
7] Pfeifer F., and Betlach M. (1985) Mol. Gen. Genet. 198, 449-455.
8] Pfeifer F. (1986) System. Appl. Microbiol. 7, 36-40.
9] DasSarma S. (1989) Can. J. Microbiol. 35, 65-72
10] Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp.A., Soppa J., Tittor J., Oesterhelt D. (2008) Genomics 91, 335-346
2] Simsek M., DasSarma S., RajBhandary U.L., and Khorana H.G. (1982) Proc. Natl. Acad. Sci., USA 79, 7268-7272.
3] Pfeifer F., Betlach M., Martienssen R., Friedman J., and Boyer H.W. (1983) Mol. Gen. Genet. 191, 182-188.
4] DasSarma S., RajBhandary U.L., and Khorana H.G. (1983) Proc. Natl. Acad. Sci., USA 80, 2201-2205.
5] DasSarma S., RajBhandary U.L., and Khorana H.G. (1984) Proc. Natl. Acad. Sci., USA 81, 125-129.
6] Simsek M. (1985) in Alaeddinoglun, G., Demain, A.L., and Lancini, G. (Eds) Plenum Press, N.Y. pp 9-17.
7] Pfeifer F., and Betlach M. (1985) Mol. Gen. Genet. 198, 449-455.
8] Pfeifer F. (1986) System. Appl. Microbiol. 7, 36-40.
9] DasSarma S. (1989) Can. J. Microbiol. 35, 65-72
10] Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp.A., Soppa J., Tittor J., Oesterhelt D. (2008) Genomics 91, 335-346