ISFtu1

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF357005 ND Francisella tularensis
Francisella tularensis subsp. tularensis Schu S4
DNA section
IS Length : 1208 bp

Ends


IR Length : 16/17

IRL : TATAGTTAATCCGAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGA
IRR : TATAGTTAATCCGAGAGTATTTTGTTAAACACATAAGAGATAGCATCACA

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TATAGTTAATCCGAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATA
TTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTG
TAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGAAAAAGATATTTGTAGA
AATGTTATAATGTCTAATAAAAATGCCATCATATAGCCAAGATTTTAGAGACATCGTAATTAATAAACATGAAGAAGGTATGACGGAGTTCGAGCTGAGT
AAGTTTTTTAACATAGATAAGCGTACAGTTGTTTCATGGATAGAGTTTTATAAAAGAACCGGAGATTATAGTTCAAAGCAAGGAGTTGGTTGTGGCAGAG
TCGCTAGCTTTACCGATAAAACATTGATTGAACAGTATTTGATAGATCATCCAGATGCAAGTGCATTAGATATAAAAGAAGCATTAGCCCCTGATATTCC
AAGAAGTACATTTTATGATTGTCTTAATAGACTTGGTTTTAGTTTTAAAAAAAGACTCCAAAATATAAGCAAAGAAAAGAACATGAAAGGTTGGAGTATA
TAGAAAAACTAAAAGAAATAGCTCAAAACTTGTTATTTTATATAGATGAGATGGGGTGTGACAATAAGCTTTCTATCCTAAGAGGATGGTCACTAATTGG
TGAGCCTAGTTATGGTGAGGTTTTAGCATATCAAACACAAAGAAGAAGTATTGTTGCTGGATATAATTATGCAGATAAAAAGATTATAGCTCTATTAGAG
TACAGCGGATATACCAATACTGAAATTTTTAATCAATGGTTTGAGGAACACTTATGCCCATCATTAAAACCTAAAACTACTATAGTAATGGATAATGCTA
GTTTCCATAAATCCTCTAAGCTGATTGAAATAGCCAATAAATTTGATGTACAAATATTATATCTACCTCCGTACTCTCCAGATTTAAATCCTATTGAAAA
GGTTTAGGCTAACTTTAAAAAAATATTTAGAAAAGTGAATAATAGTTTTGAAAAATTTTGTGATGCTATCTCTTATGTGTTTAACAAAATACTCTCGGAT
TAACTATA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
381 bp126 aa323703+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPSYSQDFRDIVINKHEEGMTEFELSKFFNIDKRTVVSWIEFYKRTGDYSSKQGVGCGRVASFTDKTLIEQYLIDHPDASALDIKEALAPDIPRSTFYDC
LNRLGFSFKKRLQNISKEKNMKGWSI

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
459 bp152 aa6491107+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKTPKYKQRKEHERLEYIEKLKEIAQNLLFYIDEMGCDNKLSILRGWSLIGEPSYGEVLAYQTQRRSIVAGYNYADKKIIALLEYSGYTNTEIFNQWFEE
HLCPSLKPKTTIVMDNASFHKSSKLIEIANKFDVQILYLPPYSPDLNPIEKV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
785 bp261 aa3231107+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPSYSQDFRDIVINKHEEGMTEFELSKFFNIDKRTVVSWIEFYKRTGDYSSKQGVGCGRVASFTDKTLIEQYLIDHPDASALDIKEALAPDIPRSTFYDC
LNRLGFSFKKKTPKYKQRKEHERLEYIEKLKEIAQNLLFYIDEMGCDNKLSILRGWSLIGEPSYGEVLAYQTQRRSIVAGYNYADKKIIALLEYSGYTNT
EIFNQWFEEHLCPSLKPKTTIVMDNASFHKSSKLIEIANKFDVQILYLPPYSPDLNPIEKV

 

Blast result :
Comments
ISFtu1 is 50%(ORF1)and 56% (ORF2) aa similar to IS630-Spn1(aa8 to 110 ; aa154 to 257).
This element contains a series of 16 bp tandem repeat (18 copies).
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
File updated: July 6 2012.
References
1]Johansson,A., Goransson,I., Larsson,P. and Sjostedt,A.(2001) J. Clin. Microbiol. 39 (9), 3140-3146