ISEnfa1
- Family IS6
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY884205 | ND | Enterococcus faecium | Enterococcus faecium Enterococcus durans |
DNA section
IS Length : 800 bp
Ends
IR Length : 19/20
IRL : GGGTTCTGTTGCAAAGTTTCCGATAAGCTCATTTTGAGGTAAAATAATTG
IRR : GGGTTCTGTTGCAAAGTCTCAAAAAGGTCATTAGTTTTTCAAGATGATTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGGTTCTGTTGCAAAGTTTCCGATAAGCTCATTTTGAGGTAAAATAATTGAAAAAGATAGCTTGGAGGAATGAAGATGACTCAGTTCAAAGGAAAACAAT
TTCAAAAAGACGTGATTATCGTAGCCGTCGGCTACTATCTTCGGTACAACCTGAGTTATCGTGAAGTGCAGGAAATTCTGTATGACCGAGGAATTCACGT
TTGTCACACCACAATTTACCGTTGGGTGCAAGAATACGGAAAGATTCTTTATCAAATTTGGAAAAAGAAAAACAAACAGTCCTTTTATTCATGGAAAATG
GACGAGACTTATATCAAAATTAAAGGAAAGTGGCATTATTTATATCGCGCTATCGATGTAGATGGCTTAACCTTAGATATCTGGTTACGGAAAAAGCGCG
ACACACAGGCTGCGTATGCTTTTCTAAAACGACTGAAGAACCAGTTTGGAGAACCAAAGGTTCTAGTAACGGATAAAGCACCCTCTATTAAGAGTGCCTT
CAGAAAAATGGACTGTATATAACAACAGAACATCGAACAATCAAGTATCTGAATAATCTGATTGAACAAGACCATCGTCCAATTAAGCGAAGAAACAAAT
TTTACCAAAGTTTGCGAACAGCCTCAACCACGATTAAAGGCATGGAAGCGATTCGAGGAATTTATAAAAAAAGCCGAAAAGAAGGGTCTCTTTTTGGCTT
TTCCGTCTGTACAGAAATCAAAGGACTATTAGGAATCCCTGCTTAAATAAGAATCATCTTGAAAAACTAATGACCTTTTTGAGACTTTGCAACAGAACCC
TTCAAAAAGACGTGATTATCGTAGCCGTCGGCTACTATCTTCGGTACAACCTGAGTTATCGTGAAGTGCAGGAAATTCTGTATGACCGAGGAATTCACGT
TTGTCACACCACAATTTACCGTTGGGTGCAAGAATACGGAAAGATTCTTTATCAAATTTGGAAAAAGAAAAACAAACAGTCCTTTTATTCATGGAAAATG
GACGAGACTTATATCAAAATTAAAGGAAAGTGGCATTATTTATATCGCGCTATCGATGTAGATGGCTTAACCTTAGATATCTGGTTACGGAAAAAGCGCG
ACACACAGGCTGCGTATGCTTTTCTAAAACGACTGAAGAACCAGTTTGGAGAACCAAAGGTTCTAGTAACGGATAAAGCACCCTCTATTAAGAGTGCCTT
CAGAAAAATGGACTGTATATAACAACAGAACATCGAACAATCAAGTATCTGAATAATCTGATTGAACAAGACCATCGTCCAATTAAGCGAAGAAACAAAT
TTTACCAAAGTTTGCGAACAGCCTCAACCACGATTAAAGGCATGGAAGCGATTCGAGGAATTTATAAAAAAAGCCGAAAAGAAGGGTCTCTTTTTGGCTT
TTCCGTCTGTACAGAAATCAAAGGACTATTAGGAATCCCTGCTTAAATAAGAATCATCTTGAAAAACTAATGACCTTTTTGAGACTTTGCAACAGAACCC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 453 bp | 151 aa | 70 | 522 | + | No |
Description :
ORF sequence :
MKMTQFKGKQFQKDVIIVAVGYYLRYNLSYREVQEILYDRGIHVCHTTIYRWVQEYGKILYQIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDVDGL
TLDIWLRKKRDTQAAYAFLKRLKNQFGEPKVLVTDKAPSIKSAFRKMDCI
TLDIWLRKKRDTQAAYAFLKRLKNQFGEPKVLVTDKAPSIKSAFRKMDCI
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 249 bp | 83 aa | 498 | 746 | + | No |
Description :
ORF sequence :
QKNGLYITTEHRTIKYLNNLIEQDHRPIKRRNKFYQSLRTASTTIKGMEAIRGIYKKSRKEGSLFGFSVCTEIKGLLGIPA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 677 bp | 228 aa | 70 | 746 | + | Yes |
Chemistry : DDE
ORF sequence :
MKMTQFKGKQFQKDVIIVAVGYYLRYNLSYREVQEILYDRGIHVCHTTIYRWVQEYGKILYQIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDVDGL
TLDIWLRKKRDTQAAYAFLKRLKNQFGEPKVLVTDKAPSIKSAFRKIQKNGLYITTEHRTIKYLNNLIEQDHRPIKRRNKFYQSLRTASTTIKGMEAIRG
IYKKSRKEGSLFGFSVCTEIKGLLGIPA
TLDIWLRKKRDTQAAYAFLKRLKNQFGEPKVLVTDKAPSIKSAFRKIQKNGLYITTEHRTIKYLNNLIEQDHRPIKRRNKFYQSLRTASTTIKGMEAIRG
IYKKSRKEGSLFGFSVCTEIKGLLGIPA
Blast result :
Comments
Contains putative programmed ribosomal frameshift (502..508).
ISEnfa1 is 94%(ORF1) aa similar to IS946V and 94% (ORF2) aa similar to IS1216V. The ORF3 is the fusion ORF and it is 94% aa similar to IS1216E.
ISEnfa1 is 94%(ORF1) aa similar to IS946V and 94% (ORF2) aa similar to IS1216V. The ORF3 is the fusion ORF and it is 94% aa similar to IS1216E.
References
1] Janet M Manson (2005) Direct submission.