ISEfa4

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AY082011 ND Enterococcus faecium
Enterococcus faecium 10/96A
DNA section
IS Length : 1896 bp

Ends


Left end : ACAGAATTAGGCAGAAGTCGCCCATCCTCTATAGGTGGGTGATGAATGCCGTTCGGTATGAGGGTTACCGTTGGTAACTCGAAGACCGACAACTAAGGCG II struct. : Yes
Right end : GTTAGTAAAGAAATACGCTATCAAGTATGCTCTATTCCCAAGAAGCTCCCACTTCAAGCGTTAAGAACCGTCAGGTTTAGCTAAGTGGTGAGTAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CTATTTACTTTTTAAAACAGACTGTCCGTCAC

DNA sequence

ACAGAATTAGGCAGAAGTCGCCCATCCTCTATAGGTGGGTGATGAATGCCGTTCGGTATGAGGGTTACCGTTGGTAACTCGAAGACCGACAACTAAGGCG
TATTGCCTTTTTATTTTGTTCCATGTTACAATCAGTCTATACAAGAAATGAGTTGATATAACATGGAATTAGATACAAATAATCATTCAGTATTTCTTCT
TTATTACCACCTTGTTTTAGTAACGAAATATCGTAGACAAGTGATTGATGATGAAATATCTGATTATGCCAAAACTACTTTTGAAAGAATTTCAGAATCG
TATCACATTACTTTAGTGGAGTGGAATCATGATAAAGACCACGTTCATATTATGTTCAAAGCTCAACCTAAAACAGAATTGACAAAATTCATCAATGCCT
ATAAAAGTGCCAGCTCAAGATTGATAAAACGTGACTTTCCAAGAGTCAAACAATTTCTTTGGAAAGAAATGTTCTGGTCTAAAAGTTTTTGTCTTCTGAC
AACTGGTGGCGCACCAATTGATGTGATTAAGAAGTATATCCAAAATCAAGGAAACAATCATAAATAGAAAGTAGGTGAACTTTATGGAACAACTAAAAGC
ATACAAATTCAGAATCTATCCAACAGAGGAACAAGAAATCTTCTTTGCCAAATCTTTTGGTTGTGTCCGTAAGGTCTACAATCTAATGCTTGATGACCGA
AAAAAGGCGTATGAAGAAGTTAAAAATGATTCTTCTAAAAAAATGACTTTTCCGACACCAGCTAAATACAAGAAAGAATTTCCGTTTTTGAAAGAAATTG
ATAGCCTTGCTTTAGCCAATGCCCAACTTAATTTAGATAAAGCATACAAGAATTTTTTTCGGGATAAATCCGTTGGATTTCCACGTTTCAAAAGTAAGAA
AAATCCTGTTCAGAGTTATACAACGAATAATCAAAATGGTACAGTTGCTTTGATTGATAGTAAATTCATCAAAGTTCCTAAATTGAAGTCATTAGTAAGA
ATTAAGCTTCATAGACAACCAAAAGGGATTATCAAATCTGCTACAATATCACGTCATTCTAGCGGTAAATACTATATTTCTTTGTTGTGTAAAGAAGAAA
TTATCGAATTACCTAAAACTAATTCCACAATTGGTATTGACTTAGGTATTATGGACTTTGCGATTCTTTCTGATGGACAAAAAATTGATAATAATAAATT
CACGTCCAAAATGGAAAAGAAACTAAAGCGTGAACAACGCAAATTGTCTAGGCGTGCTTTATTGGCTAAAAATAAAGGTACCAATCTATTTGAAGCTAAA
AATTATCAAAAACAAAAACGCAAAGTAGCTAGATTGCATGAAAAAGTAATGAATCAACGTACTGATTTTCTGAATAAGTTGAGTACAGAAATGATCAAAA
ATCACGATATTATCTGTATTGAAGACTTAAACACAAAAGGTATGTTGCGAAATCATAAATTAGCTAAAAGTATTTCTGATGTTTCTTGGTCAAAGTTTGT
GACTAAATTACAATACAAGGCTGACTGGTATGGACGAAAAATCATCAAAGTAGATAAATGGTTTCCGTCTAGTCAGATTTGTTCAGAGTGCGGACATAAA
GATGGCAAGAAATCTCTTGAAATTCGAGAATGGACTTGTCCTGTTTGTCATACTCATCATGATCGTGATATCAATGCTAGTATCAATATTCTGACCGAAG
GTCTAAGACTACATTCTATGGGATTGGCTTAGATCCAATCATCAAGAACCGTAGGAACTACGGGGATAGCTTGGTAAATAAGAGACACCGCTGTTAGTAA
AGAAATACGCTATCAAGTATGCTCTATTCCCAAGAAGCTCCCACTTCAAGCGTTAAGAACCGTCAGGTTTAGCTAAGTGGTGAGTAGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
405 bp134 aa167571+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW
KEMFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1149 bp382 aa5881736+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEEVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFP
RFKSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQK
IDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDV
SWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA

 

Blast result :
Comments
ISEfa4 is 62% (ORF1) aa similar to IS606 and 66% (ORF2) to IS1341.
References
1] Depardieu,F., Reynolds,P.E. and Courvalin,P. (2002) Direct submission GenBank.