ISfinder Mirror Site

ISEc8

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_004431	ND	Escherichia coli	Escherichia coli O157:H7 EDL933

DNA section

IS Length : 2442 bp

Ends

IR Length : 18/22

IRL : GTAAGCGTACAGCCTGAACCGTCTGGTCAGAATCTGACGAATTAGACAAA
IRR : GTAAGCGTACAGCGAGGGCCGTATTGACGGGGATGTGTTATTCAGCTGGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTCCCGACGT	CAACAAAA	CCCCTTTTAA	8
CGCCTGGATG	AGTGATTG	ATGATAGTTT	8
GTGAAGTTCA	GCGTAATC	GGGGCAGACG	8
CTTAAAGACA	CGAAAAAC	AACTTTTTAT	8
GCTACTGCCG	GAACAACC	AGCAAACGGG	8
TTGATACATC	AGTAGCTC	TGGGTATGAA	8
GCGCCGGTGA	GACATCAG	CGTCATACAG	8

DNA sequence

GTAAGCGTACAGCCTGAACCGTCTGGTCAGAATCTGACGAATTAGACAAAGTGGTGTCCACCAAATAAGTAGTGGGAACCAAAGTATCAGATATGCAGAA
AAATGTGACTCCCGGCAGGCGAAAAGGCTGCCCTAATTATCCTCCCGAATTTAAACAGCAGCTCGTTGCTGCCTCCTGTGAACCCGGGATATCCATCTCA
AAACTTGCTCTTGAAAATGGCATTAACGCCAATCTGTTGTTCAAATGGCGACAACAATGGCGCGAGGGAAAGCTGCTATTACCTTCTTCAGAGAGCCCCC
AGCTACTTCCTGTGACTCTCGATGCAGCTGCCGAACAGCCAGAATCGCTCGCAGAGGACCCGGAAACCCTCAGTATCAGCTGTGAGGTAACGTTCCGGCA
CGGGACGCTCCGCTTCAATGGCAATGTCAGCGAAAAGCTCCTGACTCTGCTGATACAGGAACTGAAGCGATGATCCCGTTACCTTCCGGGACCAAAATTT
GGCTGGTTGCCGGTATCACCGATATGAGAAATGGCTTCAACGGCCTGGCTGCGAAAGTACAAACGGCGCTGAAAGACGATCCCATGTCCGGCCATGTTTT
CATTTTCCGGGGCCGCAGCGGCAGTCAGGTTAAACTGCTGTGGTCCACCGGTGACGGACTGTGCCTCCTGACCAAACGGCTGGAGCGTGGGCGCTTCGCC
TGGCCGTCAGCCCGTGATGGCAAAGTGTTCCTTACGCAGGCGCAGCTGGCGATGCTGCTGGAAGGTATCGACTGGCGACAGCCTAAGCGGCTGCTGACCT
CCCTGACCATGCTGTAAATCTCTTTATCCTGGTTGTCACAGAATAAGCCCGGTAAAATACGGGCTTATGAACGACATCTCTTCTGACGACATCTTCCTGC
TGAAACAGCGCCTGGCCGAACAGGAAGCGCTGATCCACGCCCTGCAGGAAAAGCTGAGCAACCGGGAGCGCGAAATAGACCATCTGCAGGCGCAGCTGGA
TAAACTCCGCCGGATGAACTTCGGCAGTCGTTCCGAAAAAGTCTCCCGCCGTATCGCACAAATGGAAGCCGATCTGAACCGGCTTCAGAAAGAGAGCGAT
ACGCTGACTGGTAGGGTGTATGACCCGGCAGTACAGCGTCCGTTGCGTCAGACCCGCACCCGTAAGCCGTTCCCTGAATCACTACCCCGTGACGAAAAGC
GACTGTTGCCTGCGGCGCCGTGCTGCCCGAACTGCGGCGGTTCACTGAGCTATCTGGGCGAGGATACCGCCGAACAGCTGGAGTTGATGCGTAGCGCCTT
CCGGGTTATCCGGACGGTACGGGAAAAACATGCCTGTACTCAGTGCGATGCCATCGTGCAGGCACCTGCACCTTCGCGGCCCATCGAGCGGGGTATCGCC
GGACCGGGGCTGCTGGCCCGCGTGCTGACCTCGAAGTATGCAGAGCACACCCCGCTGTATCGCCAGTCAGAAATATACGGCCGGCAAGGTGTGGAGCTGA
GGCGTTCACTGCTGTCGGGCTGGGTGGATGCATGCTGCCGGCTGCTGTCTCCGCTGGAAGAGGCGCTTCATGGCTATGTCATGACTGACGGCAAACTCCA
TGCCGATGATACCCCGGTCCAGGTACTGCTGCCGGGTAATAAGAAGACGAAGACCGGGCGGTTGTGGGCGTATGTTCGTGATGACCGCAATGCAGGGTCA
GCGTTGGCACCTGCAGTGTGGTTCGCTTACAGCCCGGACAGAAAAGGCATCCATCCGCAGACTCATCTTGCCTGCTTCAGCGGTGTGCTGCAAGCGGATG
CGTACGCCGGGTTCAACGAGCTGTATCGCAATGGTGGGATAACGGAAGCTGCCTGCTGGGCTCATGCCCGCCGAAAGATCCACGATGTGCACGTCCGCAT
CCCGTCAGCACTGACGGAAGAAGCCCTGGAGCAGATCGGTCAGTTGTACGCCATAGAGGCGGATATAAGGGGAATGCCGGCAGAGCAGCGGCTTGCTGAA
CGTCAGCGAAAAACGAAACCGTTGTTGAAATCCCTGGAAAGCTGGTTGCGTGAAAAGATGAAGACCCTGTCGCGACACTCAGAGTTGGCGAAGGCGTTCG
CGTACGCACTTAACCAGTGGCCGGCACTGACGTACTATGCGAACGATGGCTGGGTGGAAATCGACAACAACATCGCTGAAAATGCCCTGCGGGCGGTCAG
TCTGGGTCGTAAAAACTTCCTGTTCTTCGGCTCTGATCATGGTGGTGAGCGGGGAGCGCTACTGTACAGCCTGATCGGGACGTGCAAACTGAATGACGTG
GATCCAGAAAGCTACCTTCGCCATGTGCTTGGCGTCATAGCAGACTGGCCGGTCAACCGGGTCAGCGAACTGCTTCCGTGGCGCATAGCACTGCCAGCTG
AATAACACATCCCCGTCAATACGGCCCTCGCTGTACGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
402 bp	133 aa	72	473	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MGTKVSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETL
SISCEVTFRHGTLRFNGNVSEKLLTLLIQELKR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
348 bp	115 aa	470	817	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVFIFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGI
DWRQPKRLLTSLTML

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1539 bp	512 aa	867	2405	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRK
PFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQ
SEIYGRQGVELRRSLLSGWVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTH
LACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKT
LSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVS
ELLPWRIALPAE

Blast result :

Comments

This element was first sequenced as part of the region adjacent to the locus of enterocyte effacement (LEE) pathogenicity island of Escherichia coli EDL933 [AF071034, bases 6012..8453; 1], but it was not recognized as an IS element. It was subsequently identified based on its similarity to the Sinorhizobium meliloti IS element ISRm14 [2]. ISEc8 is present in 7 intact copies and 4 partial copies in the complete genome of the E. coli O157:H7 Sakai strain [3], while strain EDL933 [4] has an additional copy in the duplicated urease-tellurite island.

References

1] Perna,N.T., Mayhew,G.F., Posfai,G., Elliott,S., Donnenberg,M.S., Kaper,J.B. and Blattner,F.R. (1998) Infect. Immun. 66 (8), 3810-3817
2] Schneiker,S., Kosier,B., Puhler,A. and Selbitschka,W. (1999) Curr. Microbiol. 39 (5), 274-281
3] Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han, C.-G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. (2001)
DNA Res. 8 (1), 11-22
4] Perna,N.T., Plunkett,G. III, Burland,V., Mau,B., Glasner,J.D., Rose,D.J., Mayhew,G.F., Evans,P.S., Gregor,J., Kirkpatrick,H.A., Posfai,G., Hackett,J.,Klink,S., Boutin,A., Shao,Y., Miller,L., Grotbeck,E.J., Davis,N.W., Lim,A.,Dimalanta,E., Potamousis,K., Apodaca,J., Anantharaman,T.S., Lin,J., Yen,G.,Schwartz,D.C., Welch,R.A. and Blattner,F.R. (2001) Nature 409 (6819), 529-533