ISfinder Mirror Site

ISDsp3

Family IS6
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009455	ND	Dehalococcoides sp.	Dehalococcoides sp. BAV1

DNA section

IS Length : 1456 bp

Ends

IR Length : 28/36

IRL : GGTTCTCCTTATTTGGTGTTGCAGGGATTTATGAAGATGTAGAAAACCCT
IRR : GGTTCTCCTTTATAAGTGTTGCATGGGTTTGTAAAGGGTGATAATATTCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCAAATCTAC	CCGCAAGA	CTACCCGAGA	8

DNA sequence

GGTTCTCCTTATTTGGTGTTGCAGGGATTTATGAAGATGTAGAAAACCCTCCAGTAAAAGGGCTGGAGGGTTTTTGTTTTGGACATCAACAAGTTTGAAA
AGGTGGTCTCAGAAATGATGAAAGGAATTTTACCAATAGAATGCAAATATTGCGGTTCAAGGCATACCCGCCGTTATGGGCATAGCAGAGCTCAAAAACA
ACGTTGGTTGTGTAACGACTGTTGCCATACCTTCGTGGAAACAAGTGCCCAGCCGGGCATGAGAACACCGCCTGAACAAATCGGGGCGGCAGTCTCCATG
TTTTATGAAGGCTTGAGTTTAAGTGCTATATGCCGTCAAATGAAACAGATACATAATATAAGCCCTTCAGACGGCACAGTCTACGGCTGGATTACAAAGT
ACTCCAAAGAAGCTATTGCCAGAGCTAAAGATATTAAGCCAAAAGTAGGTGATGTCTGGCTATGTGATGAAACAGTCCTGAAGATAGCAGGCAAGAACAT
CTGGTTTTATGACATCATAGACATGAAAACCCGTTTTCTGCTGGCTTCCCATCTTTCAGACAAGCGTTATCTGGGAGATGCCCGCACTGTACTACATAGA
GCTGGTATTAAGGCAGGTAAGAGTCCTAAGGTAGTTATCACAGACAGCCTTAACAGCTATCCTCAAGCCATAGGTGATGTATTCGGGGCTGATGTAAGGC
ATATCAAGTACAAGGGAATAACCAAATCCCCCAACAATAACATCTTGGAACGCTTTCATGGCACGCTCAAAGCCCGCACAAAGGTAATGCGAGGCTTAAA
AAGCATGGAGACTGCCAGACTATTTACTGACGGCTGGCTGGTTTTCTACAACTTCATGCGTCCTCATGAGTCTCTGGATAATCAGACACCTGCCCATAGA
GCTAAGGTTCAATTGCTAAATCAGACGTGGCATGAAGTAGTCAAGACCTCTGAGTTTAAGGCTGAGCCAGTGAAATGGCTAGAGAAGCCACCTTCGTTTT
TGAAGCAGTCAATTCCCTCAAGCGTAAAGCGAGCTATCGGGCTGGGTAATAAACTTCCGAGAGGACTCAGATGATAAAAATTACATTAAAAGAATACTAT
TTTTCGGTTTTTAGTTGCGAGATTGGTTCCTTCTTGGCTATTAAACTTTTTATTGATGAAGATGACTTGATACCCAAAATTGCCTTGATTATTTTAGCTT
TACTATCTATATCTGCCTTTGTCATGCTTATCCGTAAAGGTGGCATAAACAAACGGCAATATAATCATGGCGAAAGAAAGGAAACTAAGTATCCAGAACC
AGATGCTATTAACGGTAAGTTTAGGAACAATAAAGAAGAGTAGGAGAAATCCAACGAAAATGACTAGCGCTAAAACTAATACTTCTTTACTAGGTTTAAA
CATATCAGAATATTATCACCCTTTACAAACCCATGCAACACTTATAAAGGAGAACC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
960 bp	319 aa	115	1074	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MMKGILPIECKYCGSRHTRRYGHSRAQKQRWLCNDCCHTFVETSAQPGMRTPPEQIGAAVSMFYEGLSLSAICRQMKQIHNISPSDGTVYGWITKYSKEA
IARAKDIKPKVGDVWLCDETVLKIAGKNIWFYDIIDMKTRFLLASHLSDKRYLGDARTVLHRAGIKAGKSPKVVITDSLNSYPQAIGDVFGADVRHIKYK
GITKSPNNNILERFHGTLKARTKVMRGLKSMETARLFTDGWLVFYNFMRPHESLDNQTPAHRAKVQLLNQTWHEVVKTSEFKAEPVKWLEKPPSFLKQSI
PSSVKRAIGLGNKLPRGLR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
273 bp	90 aa	1071	1343	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MIKITLKEYYFSVFSCEIGSFLAIKLFIDEDDLIPKIALIILALLSISAFVMLIRKGGINKRQYNHGERKETKYPEPDAINGKFRNNKEE

Blast result :

Comments

ISDsp3 is 47% aa similar to ISTsp1. There is a possible passenger gene (annotated as hypothetical protein, with none positive blast on NCBI) after the transposase at position 1071 to 1343.

References

1] ISfinder annotation (2011)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Lowry,S., Clum,A., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Ritalahti,K.M., Loeffler,F. and Richardson,P. (2007) Direct submission GenBank.