ISfinder Mirror Site

ISDsh3

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009952	ND	Dinoroseobacter shibae	Dinoroseobacter shibae DFL 12

DNA section

IS Length : 3472 bp

Ends

IR Length : 26/27

IRL : CGGGCTTATGTATCAAGCGCACCAAGCTTTAAGCCCGCGAAAATCTCTAT
IRR : CGGGCTTATGTATCAAACGCACCAAGCATAACACGCTGGATTCATGTATT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGTTCTGCTCCAC	CAGATCTG	CCATAACGAGATCC	8

DNA sequence

CGGGCTTATGTATCAAGCGCACCAAGCTTTAAGCCCGCGAAAATCTCTATTATGCTTGCCTTCTAGAGGCTGAGGGTAGTGCATTGAGGTTTGTCAAAAG
ACTTCTGATCTTGTTCATCGCTGCTGTCGGAGCGGCAAATTTGGCCTTTTTTGTTTACTGGTACAGCGCCTCTGAAGTCACAGCGATCATGCCCGTTAGC
CCCAACGGGTACACGATTGAAGCGTTGGCCTTGAATGTGACCATCCTTGAGATGATATTAGTTCTGGTAGGTTTTGTGTTCGCAGCGCTTGGCCTTTTCG
GGTATACTGAGATCAAGAGCGCAGCTGTGCGCGCAGCGGTTAAGGCGGCGGAGAGAGAAGCGAAAGATACAGTCTCGGAGCAGTTGAAGCTGCTTGATGC
TGTGAGGGGCCGAAGTGACACAAGCGCGCCTGAGTATGGCGGAGATTACCGCTTAGGCGACCAGACCACAGAAGGCGCAACACCAGCGAAAGACGAATGA
CTCTAACGTACCCAGAGCAGCTAGAAGTAATTCAAAAGCACCAGGCGTCCGCCCCGGTGCAAACTGTTGCGCTTGCCGATGATCTTGGTCTGAAGGTTTG
GCACGTTCCTAATTGGCCGGATGATCTGTCAGGCAAGATCGTGAAGTCCGCCGATCAAGGTGGGCCATCTGGTTATGCAATCTATGTAAATAAAGGCCAT
CACGTCAATCGTCGGCGCTTCACAACCGCCCACGAAATTGCACACTTCATTCTCCACAGGCAGTTCATAGGTGACGGTCTGGTGGACGACGGCCTGTACA
GAAGCCGCCTGAGTAACTCGATGGAAGCGCAAGCTAACAAACTTGCTGCGGACATTCTCATGCCGCGCAACCTCTTGAACCAGCATATTGCAAACGGTGT
GACGTCAGTTGCGCGATTGGCAGAGATTTTCCAAGTTTCCGAAAGCGCAATGTCGATCCGCGTCGGCGCTCCCAACTAAAGTCGAACTTCAGATACACCT
ACCGCATCCAGGCATTGCATCATGTAGGCTGCGCTGAACTTTCCTCGTGAAAGCTTGTTCCTAATGTTCGCTTCTTTGTCCCCGATCATGTCGGCGAGCT
GGGCATAGGTTACGCCTTTCCGCTTCAACTCGGCTTTCAGGAGGTTTGCGGCTTTAGCCTCCCACTCTGTTTGATCTGCCATGTACCACTCCCGGTGCAA
AAGTATCGTATCCGATACATACGCCCTTGTATGCGGTACGGCAATGTACTATATTTGATGCATAGGCATCGGATACAGTACAAATGGCACAGCACTTCCT
CCTTTCGGCGGCATCCCGCACCCTCAGCCTTCGCTCGATCTACAAGGCGGGCGAGGATGCGGCTTACAACGTGTTCTGCGAAATGCGCTGGCCTGAGACC
AATGGCGAGGCTGTGTGCCCCCGCTGCTCTCACGGTGAAACCTACTCGATCAGCACGCGCCGCAAGTTCAAGTGCAAGAACTGCCACCACCAGTTCAGCG
TCACGTCTGGCACCATCTTTGCCAGTCGGAAGATGGACTTTGTCGACCTGCTCGCGGCGATCTGCATTCTCGTAAACGCCTCCAAGGGCGTGTCCATGAT
CCAGCTTTCCCGCGATCTGGACTGCCAGTACAAGACCGCCTTCGTCCTGGCCCACAAGCTGCGCGAAGCCATGGCGCAAGAAGTTCAGACAGGCGAAGTT
CTCGAAGGTCACGTTGAGATTGATGGGGCGTACTTCGGCGGGCACATTCGTCCGGAGAACCGGAAAGAGGATCGCAAGGACCGTCGCCTGAAAGAGAACC
AGACCGGCAAGCGCCGCGTTGTCGTCGCCCTGCGCGAGCGGGAAGGCCGCACCCTGCCGTTCGTGACCAAGCTGGAAAGCGAAGGCGTTGCGCTGGCGAA
TGAGAACGTTTGCCGCATGGCGACAGTTTCTGCTGACGAGGCAAGCCACTGGGACATGCTGCACAACGGCTGGGACGTGCAGCGCGTGAACCATTCGCAG
GTCTACAGCGACCACGGCAAGCACACCAATCTGGTAGAAAGCTTCTTCTCGCGCCTGCGCCGCATGGTGCAAGGCCAACACCACCACGTCAGTCCGCAGT
ACCTCTACCAGTACGCGAACCACGCGGCGTGGCTTGAAGACAACCGCCGCAAGGACAACGGTGCGCTGGCATCTGCGCTGATTACGAACGCGATGGATGC
GCCGGTCAGCCGTCGGTGGAAAGGCTACTGGCAGCGCGCGGCGTAACAAAGTCTTGGTGTAACAACGTACTTGCGACGACAAATCATCGACCAGCTAAGA
TCTTGTTGGTAGCTTCACCGCCATGAAAGCCCAAACCATAATTGAAGTTTATGACGAAGACCTTGTGGAACTCTTCGCCAGCAGGAGGCCCGAGTTAGAA
GTGGGCACACTGCCCTTCGAGGTAAGAAAATACTTGGGCTGCTCAAGAGACACAATTTTTCTATCTGACCAGTCGGCCCGGCACATTATCCAAGCTCACG
GCGATCACATCACGAACACTCAACTGAAGCTATTACCAAAGATACTCTGTGAGGGTCTTTGGTTAAGCGACAACAGGGCAACACATGCAGTTGTGAGCTG
CAACGTTTACGAAGTTGACTACAAAACGGTTATTAAAGTCACTGAAGATCGTACGCGCACTTATGTGAAGACGCTTCACCGCACTTCAGATCGCCAAAAG
AGAGCGTTGCTAAGAAAGACCAATGTATTGCGCAACCGTTGGTAAGTGGCGGCTATGCGGTGGGGCCTGCCTGGAACCCCACAATGCGCTCCCACCCGCG
AGTGGTGCTACGGCCGGCAGAATGTCACCGTGTCACGCATAGCCTATGCCTTGGTCAGCGGCCAAGTTGCACGCTTAACGGTAGCTAATTTTGAGCACCA
CAGTCAAGCTTAAATTAAGAAATATCCTATATTTTACAGATACTTACCTAAGTCATTGAAAGAATCCACTTTTTTGCAGCCTGCCAAAAAAGGCCAACAA
GAAATCCGTCAGTTATCCACGACCTTACTCACACTCGCCAGCAAACCACTCCCCGTTCTTACGCTTGACCATCCCATGCCCCCGAAGCTGCGCTAAACAA
ACATACACGCGCTTGTATGCAGCGCCCTTCGGTAGGTCGGATCTTGCCGCCTCAACATAATCTGCGATCTGACGCCCTGTCTTCGGCCCTTCGCGCAGTG
CCTGCAAAACCAAGCGGCGCAGTTCTCCCTTCTTGAATGCCTTGGGCGTCTTGCGCGGAACGGTAAGCGCGCTGGGGTTAACGCCCCGCATCTCAAGTAG
GGCGTCTATGTGCGGAAGCGTATTGCACCCATCGCGCACCGCCTCTTGCCGCAGGAAGTGCAGCACGGAGACAATCGTATCTTCTTGGATGGGGCGGAAG
TACCTGCGCGTCATAGGGATTCAATACATGAATCCAGCGTGTTATGCTTGGTGCGTTTGATACATAAGCCCG

Protein section

ORF number : 6

ORF 1

Length		Begin	End	Strand	Fusion ORF
417 bp	138 aa	84	500	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRFVKRLLILFIAAVGAANLAFFVYWYSASEVTAIMPVSPNGYTIEALALNVTILEMILVLVGFVFAALGLFGYTEIKSAAVRAAVKAAEREAKDTVSEQ
LKLLDAVRGRSDTSAPEYGGDYRLGDQTTEGATPAKDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
483 bp	160 aa	497	979	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTLTYPEQLEVIQKHQASAPVQTVALADDLGLKVWHVPNWPDDLSGKIVKSADQGGPSGYAIYVNKGHHVNRRRFTTAHEIAHFILHRQFIGDGLVDDGL
YRSRLSNSMEAQANKLAADILMPRNLLNQHIANGVTSVARLAEIFQVSESAMSIRVGAPN

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
207 bp	68 aa	1182	976	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MADQTEWEAKAANLLKAELKRKGVTYAQLADMIGDKEANIRNKLSRGKFSAAYMMQCLDAVGVSEVRL

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
963 bp	320 aa	1284	2246	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAQHFLLSAASRTLSLRSIYKAGEDAAYNVFCEMRWPETNGEAVCPRCSHGETYSISTRRKFKCKNCHHQFSVTSGTIFASRKMDFVDLLAAICILVNAS
KGVSMIQLSRDLDCQYKTAFVLAHKLREAMAQEVQTGEVLEGHVEIDGAYFGGHIRPENRKEDRKDRRLKENQTGKRRVVVALREREGRTLPFVTKLESE
GVALANENVCRMATVSADEASHWDMLHNGWDVQRVNHSQVYSDHGKHTNLVESFFSRLRRMVQGQHHHVSPQYLYQYANHAAWLEDNRRKDNGALASALI
TNAMDAPVSRRWKGYWQRAA

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
423 bp	140 aa	2323	2745	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKAQTIIEVYDEDLVELFASRRPELEVGTLPFEVRKYLGCSRDTIFLSDQSARHIIQAHGDHITNTQLKLLPKILCEGLWLSDNRATHAVVSCNVYEVDY
KTVIKVTEDRTRTYVKTLHRTSDRQKRALLRKTNVLRNRW

Blast result :

ORF 6

Length		Begin	End	Strand	Fusion ORF
390 bp	129 aa	3414	3025	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTRRYFRPIQEDTIVSVLHFLRQEAVRDGCNTLPHIDALLEMRGVNPSALTVPRKTPKAFKKGELRRLVLQALREGPKTGRQIADYVEAARSDLPKGAAY
KRVYVCLAQLRGHGMVKRKNGEWFAGECE

Blast result :

Comments

The transposase is the fourth ORF, others are passenger genes.
ISDsh3 is 91% aa similar to ISRssp2.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.