ISfinder Mirror Site

ISCysp14

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_011729	ND	Cyanothece sp.	Cyanothece sp. PCC 7424

DNA section

IS Length : 1822 bp

Ends

Left end : CGATAGCGCGTCAGTCCCCAATTTCAGCAATAGCGAATTGGGGAAGGATAGCGCGAGAATTTGAGGGTTCAATACCCGAAAAATTCTCAACTAATCTTTT II struct. : Yes
Right end : TAGAGTCGTCAACCTCTGTTTTGTGCGGAAGTATGAGGCAAGTTGGGGCGAAGAAGAGTCAGAAACATCTGTCTCAGCGTAGCGGACAGATGTAGTTCAT II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
ATTTCCCACTTT	TGAA	AGATTTTTGTTG	tcat

DNA sequence

CGATAGCGCGTCAGTCCCCAATTTCAGCAATAGCGAATTGGGGAAGGATAGCGCGAGAATTTGAGGGTTCAATACCCGAAAAATTCTCAACTAATCTTTT
GGTGAGTCCTGATTTTTTATATATTCTTTTAACTTCTCGATAGGCGCACCCCCAGCAGAAGCCACATAATAAGAGTTTGACCAAAAAGCAACTTTTCCCC
AATAAAATTTTTTAATTTGCTCAGGAAAATCTTTTTTCATAGTTCTTGCCGTAGAACTTTTAAGACAACCTGCTACAGCAGATATAGAGTTTTTGGGATG
GAAATCTAGCAAGATATGTATATGGTCAGCCTCCCCCGAAAACTCAAGGACTTCACAATCTAATTTTCTAGATACTTGCCAAATCATCTCTCTCAACCTT
TCTAACATAGGAGTGGTTATAACTTTCCTTCGATAGTGAGTAACAAAAACAAAGTGTAACCGCACACTAAAAACACTATGAGAACTTTTTCTTGGCTTCC
GAAATGACATCCTATAGGCTTATGCTAGGATTTAAGTATAACAGTTTAAGTCGATGTGTTATGGCTACCAAGCGAATTACTTTTCGTCTTTATCCCAACA
AAGCCCAAAATGATAAGCTACATTATTGGCGCAAGCTACATTGCTTGCTATATAATGCTTGCGTTTACCATCGTAAAACCGAGTACAAAAAATTTGGAAA
AAGTGTTAACTACTTTGACCAACAAAACTGCTTACCAGAGTTTAAAAAATGTTGGACGGAATACCTAGAACTGGGTAGCCATGCACTTCAAGCAACTGTT
AAACGGGTTGATTTTGCTTTTCAGAGGTTCTTTAAACTTAAATTAGGGTATCCGAGATTTAAGTCTTCTAGGCACTATAAAGGTTGGACTTATCCTGAGC
AAGCTGGTTGGAAAATAGAATCTAGTGGGCATCATGGATTTTTAAACATCTCTAAACTAGGGAGAATTAAAATCAGGGGTAAAGCTAGGGATTGGGGAAT
ACCAAACACTTGCACAATAATGTTTAAGCAAGGTAAATGGTTTGCCTCAATCACCGTAGAGTGTAACCCAATTCGTCCCACTACTAATACAGGAGCTATC
GGATTAGATTTTGGCTTAAATCATGCGGTTGCTGACTCTAACGAAAATTTTATAGAAAATCCTAGATTCCTAAAAGTGGCTCAAGAAAAGATTAAAAAGG
CTGCCAAAAAAGCTAGGCGTAAACGCAACCCTAAAAGAGGAGTTAGAGCATCTAGAAGGTGGAAAAAAGCAGCTAGAAAAGTAGGGAAAATTCAGAGCAA
GGTTGCCAGACAAAGGCAAGACTGGCAACACAAAGTAGCGGTAGAAATAGTTAGCCGTAATAGCTTCATCGCTACTGAAAAATTAACCCTAAAAGGGATG
ACCAAAAAAGCCAAGAAAGGTAGCAAGCGTAAAAAACAAAAATCAGGGTTAAATAAGTCTATTCTTGACGTAGGAATAGGCAATTTAAAAGCCTTGATTA
AAGAAAAAGTTACCGATGCCGGTGGTTTTTATATAGAAGTGCCCACTACTAAAGTAAAACCATCACAAACTTGTAGCAATTGCGGTCATCAAAAGAAAAA
AACATTAGCAGAAAGAATCCATGTTTGTGAGCGTTGTAATTTCACTTGTAATAGAGACACGAACGCAGCAAAAGTGATGTTAAATTATGCTAAAAGGGGG
CAGGAACTGCCCTCTACAGACTTAGAGTCGTCAACCTCTGTTTTGTGCGGAAGTATGAGGCAAGTTGGGGCGAAGAAGAGTCAGAAACATCTGTCTCAGC
GTAGCGGACAGATGTAGTTCAT

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
420 bp	139 aa	510	91	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MSFRKPRKSSHSVFSVRLHFVFVTHYRRKVITTPMLERLREMIWQVSRKLDCEVLEFSGEADHIHILLDFHPKNSISAVAGCLKSSTARTMKKDFPEQIK
KFYWGKVAFWSNSYYVASAGGAPIEKLKEYIKNQDSPKD

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1296 bp	431 aa	522	1817	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLGFKYNSLSRCVMATKRITFRLYPNKAQNDKLHYWRKLHCLLYNACVYHRKTEYKKFGKSVNYFDQQNCLPEFKKCWTEYLELGSHALQATVKRVDFAF
QRFFKLKLGYPRFKSSRHYKGWTYPEQAGWKIESSGHHGFLNISKLGRIKIRGKARDWGIPNTCTIMFKQGKWFASITVECNPIRPTTNTGAIGLDFGLN
HAVADSNENFIENPRFLKVAQEKIKKAAKKARRKRNPKRGVRASRRWKKAARKVGKIQSKVARQRQDWQHKVAVEIVSRNSFIATEKLTLKGMTKKAKKG
SKRKKQKSGLNKSILDVGIGNLKALIKEKVTDAGGFYIEVPTTKVKPSQTCSNCGHQKKKTLAERIHVCERCNFTCNRDTNAAKVMLNYAKRGQELPSTD
LESSTSVLCGSMRQVGAKKSQKHLSQRSGQM

Blast result :

Comments

ISCysp14 is 92% (ORFA) and 84% (ORFB) aa similar to ISCysp13.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2008) Direct submission GenBank.