ISCot1

  • Family ISKra4
  • Group ISAzba1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AEWF01000011 ND Candidatus Odyssella
Candidatus Odyssella thessalonicensis L13 HMO
DNA section
IS Length : 2802 bp

Ends


IR Length : 25

IRL : GAGACCATCCCAAAGTTGGGGGTCACATTGATTTTGTTAAGTTTCCAGGT
IRR : GAGACCATCCCAAAGTTGGGGGTCATGCCGCTAAAGGTTGAGGAATATTA

Insertion site


Left flankDirect repeatRight flankDR Length
GGTAGATAGAAATATATT0

DNA sequence

GAGACCATCCCAAAGTTGGGGGTCACATTGATTTTGTTAAGTTTCCAGGTTTCGATATATAGTTGATGGGTGGGTATTCAAAGTTTTAGCTATACTCTTA
ATGGACTCACCTTCCGAGAGTAGACGTTTCATCAGGCTCTTTTGTTCTGAGTTGAGCTTAGAGGGACGCCCGAACTTAACACCTCTTTTTTTCGCTAAAG
AGCGACCAGTACTCGTCCGCTCTTTAATTAATTCCCTTTCAAATTCAGCAATGCCTGTAAATACCGTCATCACCATACGACCGGCATGAGATGTTGTATC
AGCCCATGGTTCACACAGTGAGCGAAAATGAGCCCCTACGTGATGGAGTTTGTCAGTTATTTCAAGTAGATTTCTTGTAGAGCGAGCTAATCGATCCAAG
CGCCATACAATAATAGTGTCATTTTCCCGTAATTGCTCCAGCATTTTTTCTAGCTCAGGTCTTTTCATATGAGCTCCTGAAACTTTTTCCTGGTAAATTT
TTTTACACCCTGCTTCCTTTAACACCTGGAGCTGAAAGGTTAAATCTTGGTCGTCAGTAGAAACCCGTGCATATCCAATTTTCATAAAGCCCTCTAGGAG
AAATTTTCTTTGCAAAACATATAGCAAAATATTTTACTTCTGCATATGGTTTTTGCAATGAGAAAGGATCAGTACTTTCCTAACTCCATCCTGTTATTGC
AAAAGTTACAATTTTTGCGAACTTAAAAAGGAACTCTCATCAATGACCCCACAGTCCCCTATAGAGGTTTATCAGTTTAAATTTTTTCTTAAAGGGATTA
GCCCTATGATTTGGAGACGAATTCTAGTGAAGAGCAATAGTAGCCTTGCAGATCTTCATTATGCTATCCAAATTACTATGGGGTGGAGCGATACACATCT
TCATCAATTTGATATTTGGGGAAAACAATATGGTCTTTCTTATGACGGAGGAATCATTTTCCCTGATAAAGCCAGAGAAGTTTATTTACAACATTTCCAA
TTTCGAGTTAACGAAAAATTTATCTATAAATATAATTTCTTCGCTCCTTGGGAACATGAAATTCGCCTTGAAAAGAAGCTATTACTTGATTCTAAAAAAA
CATATCCACTTTGCATTGCTGGCAATTACGTTAGCCCTCCTGAAGACTGCGGGGGCCCTAAGGCTTTCATGGAATTGATAGACTATTATTCTGTCTGGAG
AATTGAAGAAGAAATCTTAGAGGCATTAGAAGAGTATGAAGCTGAAAGAGATCCTGCTTCTTTTAAAGAAACCTTGGAAAATCTTCAATACTGGGCAAGC
CGCCACAAATTTGACCGTAAAAAAATTAATCGACAACTACAGCGTTATTTCAACAACCCAAGCAATAATCAATTAACACTGGAGGAGATTCAAGATGAAG
ATTAAAATCCAATTTATCATTGATAGTGAAGACCAAAGCTCAACAGAAGTTATAGCCTGCATTGAGAGAGAAGAATTAACAGCTGAAACTTTAGGATTAA
CTCTACAAGAGGCTAAGACGATTACCTCTGAAATTCAGAAAAAGATGATAGGTCATCAAATAACAGACTTTGTTTCTCATAACCGTTTTTGCCTATGTTG
CGGTTCAACCCTCTCTATTAAGGGGTACCATCCTTTAGTTTATAGGACTCTTTTTGGTAAGATTTGTCTAAGAAGCCCGAGGCTTTTTTCTTGCCGATGC
CAATCTCAGCAATCCTCAACCTTTAGCCCTTTGGTTCAGCTCTTAACAGAACACGTGTCACCTGAACTAAGTTATTTGGAGTCAAAATGGGCGTCATTGA
TGTCGTATGGATTGGCTGCTAAGCTTCTAAAAGAGGTATTCCCTGTGGAAATTTATCCTTCTTCGGTATACCGCATTACCTCCAAAGTAGCCAACCGCTT
AGAACAAGAATTAGGCGAAGAAAAAGCTATGTTCATTAAGGGATGCCAGCGGGATTGGAATCAACTGCCCCGGCCAGATACCCCTATTACCGTTAGCCTT
GATGGAGGGTACGTACATGCAAGGAAAGGAAATAACCGTAAGGCTGGGTGGTTTGAGGTTATTGTTGGTAAAAGCTTGCAAGAAGGTCATCAACCAAGAA
GGTTTGGTTATGTTGTTAATTATGATCAAAAGCCAAAACGTCGGTTGTATGAAATGCTTGAAAAACAAGGATTACAATTGAATCAAGATATTACTTTCCT
AACTGATGGTGGCGATACAGTGCGAGGTCTACCTCTTTATCTAAGTCCTCGATCAGAGCATATTTTAGATTGGTTTCATGTGACCATGCGGATGACTGTT
ATAAAACAAATTTCAAAGGGAGCTATGGGCAAAGACTATGCGAACTTTGAAAAACAGCTATTAAGGACAAAGTGGTTTCTTTGGCATGGGAATATCTATC
AGTCCTTGGATATCTTGGAATCTTTAATAATGGATTTAGACTTATATTCCGAAAATAAAGCACACAAAAAATATAAGCTTTGGCGGGTTGTCAGTGAGTT
TTATCAGTATATTCAATCAAATAGTCACTTCATTCCCAATTATGGGGAACGTTATCGTTACGGTGAATCAGTCTCCTCAGCTTGCGCTGAATCAGCAGTC
AATGAGCTGATTAGCAAGAGGATGGCTAAAAGCCAACAAATGAGATGGACACAGAAAGGAGCTCATCTACTGCTTCAAGTCCGTTCGAAGACCCTAAATG
GTGACTTAATAAAATCCTTTAAACAATGGTACCCAAAAATGGATGATGCCCATAATATTCCTCAACCTTTAGCGGCATGACCCCCAACTTTGGGATGGTC
TC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
549 bp181 aa58537-No
ORF function : Passenger Gene
Annotation : Serine recombinaseDescription :

ORF sequence :

MKIGYARVSTDDQDLTFQLQVLKEAGCKKIYQEKVSGAHMKRPELEKMLEQLRENDTIIVWRLDRLARSTRNLLEITDKLHHVGAHFRSLCEPWADTTSH
AGRMVMTVFTGIAEFERELIKERTSTGRSLAKKRGVKFGRPSKLNSEQKSLMKRLLSEGESIKSIAKTLNTHPSTIYRNLE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
663 bp220 aa7431405+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTPQSPIEVYQFKFFLKGISPMIWRRILVKSNSSLADLHYAIQITMGWSDTHLHQFDIWGKQYGLSYDGGIIFPDKAREVYLQHFQFRVNEKFIYKYNFF
APWEHEIRLEKKLLLDSKKTYPLCIAGNYVSPPEDCGGPKAFMELIDYYSVWRIEEEILEALEEYEAERDPASFKETLENLQYWASRHKFDRKKINRQLQ
RYFNNPSNNQLTLEEIQDED

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1386 bp461 aa13952780+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKIKIQFIIDSEDQSSTEVIACIEREELTAETLGLTLQEAKTITSEIQKKMIGHQITDFVSHNRFCLCCGSTLSIKGYHPLVYRTLFGKICLRSPRLFSC
RCQSQQSSTFSPLVQLLTEHVSPELSYLESKWASLMSYGLAAKLLKEVFPVEIYPSSVYRITSKVANRLEQELGEEKAMFIKGCQRDWNQLPRPDTPITV
SLDGGYVHARKGNNRKAGWFEVIVGKSLQEGHQPRRFGYVVNYDQKPKRRLYEMLEKQGLQLNQDITFLTDGGDTVRGLPLYLSPRSEHILDWFHVTMRM
TVIKQISKGAMGKDYANFEKQLLRTKWFLWHGNIYQSLDILESLIMDLDLYSENKAHKKYKLWRVVSEFYQYIQSNSHFIPNYGERYRYGESVSSACAES
AVNELISKRMAKSQQMRWTQKGAHLLLQVRSKTLNGDLIKSFKQWYPKMDDAHNIPQPLAA

 

Blast result :
Comments
ISCot1 is 63% aa (ORFC : the transposase) similar to ISAzs26.
ORFA and ORFB are passenger gene respectively annotated as resolvase family site-specific recombinase and hypothetical protein.
References
1] ISfinder annotation (2013)
2] Kalliopi,G., Madoui,M.-A., Le,P., Robert,C. and Raoult,D. (2011) Direct submission GenBank.