ISfinder Mirror Site

ISClph1

Family IS1595
Group ISPna2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010001	ND	Clostridium phytofermentans	Clostridium phytofermentans ISDg

DNA section

IS Length : 1607 bp

Ends

IR Length : 22/24

IRL : GGCTCTGTCACAACATTTTGTTGATATTCAGAACATTAGTTTGTAAGTTC
IRR : GGCTCTGTCACAACATACTGTTGAAAATTAGTGATATTTAAGCAGAGCTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCTCCTTTCTTT	AATTTTTT	TAGTAAAAATTATTC	8

DNA sequence

GGCTCTGTCACAACATTTTGTTGATATTCAGAACATTAGTTTGTAAGTTCTTTTTAGTGTGTTATAATACCCTTATAAACTGTAAAAGGAGGGATATTAT
ATGCCAGATATTAAAGACGTTTTTACACTGTATACTCAGCTTTCACCACAAGAACAGAAAAAGCTATACTCAATGATTTCATATCAACTACCTATTGATA
ACGATTTTGAGCAATTTGTCAAAGATGAACGCTTTACGTCTGGATTCGTATGCCCCCATTGCGGTTGTATTGGTCATATTTCAAGAAATGGTCATATTTA
CAAAACTGATAAAGACGGAAACAAAACCATTGATAAACAACGTTATGTCTGTAAGGACTGTGGAAAATCCTTTACTGTATTATCTAACTCAGTTGCATCT
GGAACACGCAAGAATCTTAGTATTTGGGAATCATTCACTAAATGTATGTTGAATGGTTTTTCTGTACGTAAATCTGCGAATATTTGTGGTATTCACCGAA
ATACAGCTTTTGCATGGAGACATAAACTCCTGGATGCCCTCCAGCAACCTGCTGAAAAAACAAGATTGGATGGTATTATAGAAGCAGATGAAACCTTTTT
TCGTGTTTCATATAAAGGCAATCATAAGAGAAGTTCCTTTAAAATACCGAGAGAAGCTCGCAAACGTGGTGGTTTAGCACATAAAAGAGGATTATCAAAA
GAGCAGGTCTGTGTCCCATGTGCTATTGACCGAAAAGGTCATTCTTATGGTAAAATAGCATCATTAGGAAGGATAGCAACTAAGAATTTACATGCTGTTT
ATGATGGAAAAATCTGTTCTGAATCAACTCTTTGTACGGATAAAATGAATGCTTATGTTAGATTTGCTAAAAGTAAAAATATTCAGTTAATTCAGCTAAA
ATCAGGTAAAGAGAAAAAGGGAATTTACAATATTCAAAGAATCAATAATTATCATACAAAGCTTAAGAAGTTCATGGATAAATTTAATGGTGTGGCTACA
AAATACCTTAACAACTATCTTGTATGGCATACTTGGTTTGACTTAAAAGGTCATACACAACTTGAAAAAGCTGATTTGCTCTTAGCTAAAGCAATGACAA
GTATTCTTTCTTTTCACTTCAGACAATTGTCAATGCGTCCATCAATACCATTAAATATCTGAATTTGAGTTATTTTAAATATAGAAGAATTATTAAACTA
ATGGATAGAATGGAGGGTATATATGTTTGAATTAGTATTTCTGGTACTAATTGGGTTAATTTTTAGTTTTTTGGGGTGGCGTATCTGGAAAAAAGAACAG
ATAACACTAATCCATGATTATCACTATACTAAAGTGATTGAAAAGGACAAGAAACCATACACAGAAAAAATGGGTAAGGCATGTATTGTTATGGGAATCG
GAATGATATTGACAGGTGCAATTGATTTTATAACAACTACGTTTTATGGATGGATATTCTTTGGAGTCTGTTTTATTATCGGTTTAATTATTATGATTTT
TGCTCAATTAAAGTATAATGGTGGGTTATTCTAATAGCATTATTTAAGCAGAGTGGTAAGCTCTGCTTAAATATCACTAATTTTCAACAGTATGTTGTGA
CAGAGCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1062 bp	353 aa	101	1162	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPDIKDVFTLYTQLSPQEQKKLYSMISYQLPIDNDFEQFVKDERFTSGFVCPHCGCIGHISRNGHIYKTDKDGNKTIDKQRYVCKDCGKSFTVLSNSVAS
GTRKNLSIWESFTKCMLNGFSVRKSANICGIHRNTAFAWRHKLLDALQQPAEKTRLDGIIEADETFFRVSYKGNHKRSSFKIPREARKRGGLAHKRGLSK
EQVCVPCAIDRKGHSYGKIASLGRIATKNLHAVYDGKICSESTLCTDKMNAYVRFAKSKNIQLIQLKSGKEKKGIYNIQRINNYHTKLKKFMDKFNGVAT
KYLNNYLVWHTWFDLKGHTQLEKADLLLAKAMTSILSFHFRQLSMRPSIPLNI

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
312 bp	103 aa	1223	1534	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MFELVFLVLIGLIFSFLGWRIWKKEQITLIHDYHYTKVIEKDKKPYTEKMGKACIVMGIGMILTGAIDFITTTFYGWIFFGVCFIIGLIIMIFAQLKYNG
GLF

Blast result :

Comments

ISClph1 is 59% aa similar to ISBwe1.
The first ORF is the transposase. There is a second passenger ORF in this sequence.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Detter,J.C., Han,C., Kuske,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E.A. and Richardson,P. (2007) Direct submission GenBank.