ISCgl1
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U85507 | Y | Corynebacterium glutamicum | Corynebacterium glutamicum plasmid pXZ10145.1 |
DNA section
IS Length : 2435 bp
Ends
IR Length : 22
IRL : TGTGATGTCCAGGGACGTTGTTCTGGCCCCCGCCCGTAATGACACCGTGC
IRR : TGTGATGTCCAGGGACGTTGTTGAGTCGGTCGAAGGGTACGCCGCCAATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACGACGGAA | TCTAGC | CCAAGCGATG | 6 |
DNA sequence
TGTGATGTCCAGGGACGTTGTTCTGGCCCCCGCCCGTAATGACACCGTGCCGATTCAAGAAGTATCTGTCCAGACCGCTTGCCATTGAAGGCATGTTAGA
GTTTGGGGCATGTCGAGTCCCGAATCAGACAGGTCAGGGGTTGTCGGTCACACCGCCAGCCCCTTGAACCACTAGTTACGACGCCCACGATCTGTGTGGG
CGTATGTCTGGCGTACCCGGGGCGCTGGCCGTGGTGACAACAAGAACCATTTCTTGATCTCACACCTCGGAGTACTCGATGCCTTTTGCCCTCTACATGC
TTGCCCTGGCGGTCTTCGTCATGGGCACTTCAGAATTCATGCTCGCGGGATTGCTCCCCGCGATCGCGACCGAACTTGACGTCTCGGTCGGCACTGCGGG
CCTGCTGACCTCCGCATTCGCAGTCGGTATGGTCGTCGGCGCGCCAGTGATCGCGGCATTCGCTCGCCGTTGGCCACCGCGGCTCACATTGATCGTTTGC
CTTCTCGTGTTCGCGGGAAGCCACGTCATCGGAGCGATGACACCAGTGTTCTCTCTCCTGCTCATCACCCGGGTGCTCAGCGCTCTCGCAAACGCAGGAT
TCCTCGCCGTAGCACTGAGCACGGCCACTACCCTCGTGCCAGCGAACCAGAAGGGGCGTGCACTGTCGATCCTGCTCTCCGGCACGACGATCGCAACCGT
CGTGGGCGTCCCCGCCGGGGCACTGCTCGGCACAGCGCTGGGCTGGCGAACGACGTTCTGGGCGATCGCCATCCTCTGTATTCCCGCGGCCGTTGGAGTC
ATTCGTGGCGTCACGAACAATGTTGGTCGGAGCGAGACTAGCGCGACCTCACCAAGGCTCCGTGTCGAGCTCAGCCAGTTGGCGACGCCGCGGCTCATCC
TGGCCATGGCACTCGGAGCGCTGATCAACGGAGGGACCTTTGCGGCATTCACCTTCCTGGCACCCATCGTGACCGAGACCGCGGGCTTGGCCGAAGCGTG
GGTGTCCGTCGCGCTGGTGATGTTCGGCATCGGATCGTTCCTTGGCGTCACGATCGCAGGACGACTATCAGATCAACGACCTGGCCTCGTGCTCGCAGTC
GGCGGACCGCTATTGCTGACAGGCTGGATCGTGTTGGCAGTGGTCGCATCTCATCCCGTTGCGCTTATCGTCCTCGTCCTCGTTCAGGGATTCCTGTCGT
TCGGCGTCGGCAGTACTCTGATCACGCGTGTGCTGTATGCAGCATCGGGTGCGCCAACGATGGGCGGTTCGTACGCAACCGCAGCATTGAATATCGGAGC
TGCAGCGGGGCCCGTGCTTGGTGCGCTCGGGCTCGCGACCGGGCTGGGGCTGCTCGCGCCGGTTTGGGTCGCTTCGGTGCTGACAGCGATCGCTCTCGTC
ATCATGCTTCTCACCAGACGCGCGCTTACGAAGACCGCGGCGGAGGCCAATTGATGGCCCATCCGAACGCTCTTCTCACTCCTCGTGCCCGTCTCCGGTT
AGCTCGGCTGATTGTCGAAGACGGCTATCCGGCCACGATCGCCGCAAAGATGTTCATGGTCTCCCCGATCACTGCCCGGAAATGGGCAGGCCGCTACCGG
GAAGAGGGTGAGTTTGGGATGCAGGATCGCTCCAGCAAGCCGCACCGGATCCCAGGCAGGACGCCCGAGCATGTCAAGAAGAAGATCATCAACCTGCGCT
GGCGGCTTCGACTGGGGCCAGCCCAGATCGCTGCGCGACTTGGTCTCTCGACGTCGACTGTTCACGCGGTCCTCGTCCGTTGCCGCGTGAACCGCCTCTC
GCATATCGATCGTGTCACTGGCGAGCCATTGCGGCGATATGAGCATCCTCATCCGGGATCGTTGATTCATGTCGATGTCACGAAGTTCGGCAACATCCCC
GACGGCGGTGGACATCGTTACGTAGGTCGGCAGCAAGGCGCACGGAACAAGCTAGCGACTCCGGGATTACCACGAGGAAAAGATCACAAGCCGCGCACCG
GGACGGCGTTCGTTCACACAGTCATCGATGACCACTCCCGCGTCGCATACGCAGAAATCTGGTCGGATGAGCAGGCGAGCACAGCGGTGGGAGTTCTCGA
ACGCGCCGTGGCCTGGTTCGCCGAACGAGGCGTGACCGTCGAGCGAGTCCTATCCGACAACGGGTCGGCATACAGATCCCACGCATGGAGGGACTTCTGC
GCTCGGCTCGGCATCCGACACAAGCGGACACGCCCTACCGGCCGCAGACGAACGGGAAGATCGAGCGATTCCACGCACGCTCGGGGACGGCTGGGCCTAT
GCCAGGTTTTACGGTTCAGAGGCCGAACGACGCTGGCGCTGCCCGGCTGGCTCCACTTCTACAACCACCACCGACACCACTCTGCGATTGGCGGCGTACC
CTTCGACCGACTCAACAACGTCCCTGGACATCACA
GTTTGGGGCATGTCGAGTCCCGAATCAGACAGGTCAGGGGTTGTCGGTCACACCGCCAGCCCCTTGAACCACTAGTTACGACGCCCACGATCTGTGTGGG
CGTATGTCTGGCGTACCCGGGGCGCTGGCCGTGGTGACAACAAGAACCATTTCTTGATCTCACACCTCGGAGTACTCGATGCCTTTTGCCCTCTACATGC
TTGCCCTGGCGGTCTTCGTCATGGGCACTTCAGAATTCATGCTCGCGGGATTGCTCCCCGCGATCGCGACCGAACTTGACGTCTCGGTCGGCACTGCGGG
CCTGCTGACCTCCGCATTCGCAGTCGGTATGGTCGTCGGCGCGCCAGTGATCGCGGCATTCGCTCGCCGTTGGCCACCGCGGCTCACATTGATCGTTTGC
CTTCTCGTGTTCGCGGGAAGCCACGTCATCGGAGCGATGACACCAGTGTTCTCTCTCCTGCTCATCACCCGGGTGCTCAGCGCTCTCGCAAACGCAGGAT
TCCTCGCCGTAGCACTGAGCACGGCCACTACCCTCGTGCCAGCGAACCAGAAGGGGCGTGCACTGTCGATCCTGCTCTCCGGCACGACGATCGCAACCGT
CGTGGGCGTCCCCGCCGGGGCACTGCTCGGCACAGCGCTGGGCTGGCGAACGACGTTCTGGGCGATCGCCATCCTCTGTATTCCCGCGGCCGTTGGAGTC
ATTCGTGGCGTCACGAACAATGTTGGTCGGAGCGAGACTAGCGCGACCTCACCAAGGCTCCGTGTCGAGCTCAGCCAGTTGGCGACGCCGCGGCTCATCC
TGGCCATGGCACTCGGAGCGCTGATCAACGGAGGGACCTTTGCGGCATTCACCTTCCTGGCACCCATCGTGACCGAGACCGCGGGCTTGGCCGAAGCGTG
GGTGTCCGTCGCGCTGGTGATGTTCGGCATCGGATCGTTCCTTGGCGTCACGATCGCAGGACGACTATCAGATCAACGACCTGGCCTCGTGCTCGCAGTC
GGCGGACCGCTATTGCTGACAGGCTGGATCGTGTTGGCAGTGGTCGCATCTCATCCCGTTGCGCTTATCGTCCTCGTCCTCGTTCAGGGATTCCTGTCGT
TCGGCGTCGGCAGTACTCTGATCACGCGTGTGCTGTATGCAGCATCGGGTGCGCCAACGATGGGCGGTTCGTACGCAACCGCAGCATTGAATATCGGAGC
TGCAGCGGGGCCCGTGCTTGGTGCGCTCGGGCTCGCGACCGGGCTGGGGCTGCTCGCGCCGGTTTGGGTCGCTTCGGTGCTGACAGCGATCGCTCTCGTC
ATCATGCTTCTCACCAGACGCGCGCTTACGAAGACCGCGGCGGAGGCCAATTGATGGCCCATCCGAACGCTCTTCTCACTCCTCGTGCCCGTCTCCGGTT
AGCTCGGCTGATTGTCGAAGACGGCTATCCGGCCACGATCGCCGCAAAGATGTTCATGGTCTCCCCGATCACTGCCCGGAAATGGGCAGGCCGCTACCGG
GAAGAGGGTGAGTTTGGGATGCAGGATCGCTCCAGCAAGCCGCACCGGATCCCAGGCAGGACGCCCGAGCATGTCAAGAAGAAGATCATCAACCTGCGCT
GGCGGCTTCGACTGGGGCCAGCCCAGATCGCTGCGCGACTTGGTCTCTCGACGTCGACTGTTCACGCGGTCCTCGTCCGTTGCCGCGTGAACCGCCTCTC
GCATATCGATCGTGTCACTGGCGAGCCATTGCGGCGATATGAGCATCCTCATCCGGGATCGTTGATTCATGTCGATGTCACGAAGTTCGGCAACATCCCC
GACGGCGGTGGACATCGTTACGTAGGTCGGCAGCAAGGCGCACGGAACAAGCTAGCGACTCCGGGATTACCACGAGGAAAAGATCACAAGCCGCGCACCG
GGACGGCGTTCGTTCACACAGTCATCGATGACCACTCCCGCGTCGCATACGCAGAAATCTGGTCGGATGAGCAGGCGAGCACAGCGGTGGGAGTTCTCGA
ACGCGCCGTGGCCTGGTTCGCCGAACGAGGCGTGACCGTCGAGCGAGTCCTATCCGACAACGGGTCGGCATACAGATCCCACGCATGGAGGGACTTCTGC
GCTCGGCTCGGCATCCGACACAAGCGGACACGCCCTACCGGCCGCAGACGAACGGGAAGATCGAGCGATTCCACGCACGCTCGGGGACGGCTGGGCCTAT
GCCAGGTTTTACGGTTCAGAGGCCGAACGACGCTGGCGCTGCCCGGCTGGCTCCACTTCTACAACCACCACCGACACCACTCTGCGATTGGCGGCGTACC
CTTCGACCGACTCAACAACGTCCCTGGACATCACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1176 bp | 391 aa | 279 | 1454 | + | No |
Annotation : Chloramphenicol resistance geneDescription : Antibiotic resistance
ORF sequence :
MPFALYMLALAVFVMGTSEFMLAGLLPAIATELDVSVGTAGLLTSAFAVGMVVGAPVIAAFARRWPPRLTLIVCLLVFAGSHVIGAMTPVFSLLLITRVL
SALANAGFLAVALSTATTLVPANQKGRALSILLSGTTIATVVGVPAGALLGTALGWRTTFWAIAILCIPAAVGVIRGVTNNVGRSETSATSPRLRVELSQ
LATPRLILAMALGALINGGTFAAFTFLAPIVTETAGLAEAWVSVALVMFGIGSFLGVTIAGRLSDQRPGLVLAVGGPLLLTGWIVLAVVASHPVALIVLV
LVQGFLSFGVGSTLITRVLYAASGAPTMGGSYATAALNIGAAAGPVLGALGLATGLGLLAPVWVASVLTAIALVIMLLTRRALTKTAAEAN
SALANAGFLAVALSTATTLVPANQKGRALSILLSGTTIATVVGVPAGALLGTALGWRTTFWAIAILCIPAAVGVIRGVTNNVGRSETSATSPRLRVELSQ
LATPRLILAMALGALINGGTFAAFTFLAPIVTETAGLAEAWVSVALVMFGIGSFLGVTIAGRLSDQRPGLVLAVGGPLLLTGWIVLAVVASHPVALIVLV
LVQGFLSFGVGSTLITRVLYAASGAPTMGGSYATAALNIGAAAGPVLGALGLATGLGLLAPVWVASVLTAIALVIMLLTRRALTKTAAEAN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 982 bp | 328 aa | 1454 | 2435 | + | No |
Chemistry : DDE
ORF sequence :
MAHPNALLTPRARLRLARLIVEDGYPATIAAKMFMVSPITARKWAGRYREEGEFGMQDRSSKPHRIPGRTPEHVKKKIINLRWRLRLGPAQIAARLGLST
STVHAVLVRCRVNRLSHIDRVTGEPLRRYEHPHPGSLIHVDVTKFGNIPDGGGHRYVGRQQGARNKLATPGLPRGKDHKPRTGTAFVHTVIDDHSRVAYA
EIWSDEQASTAVGVLERAVAWFAERGVTVERVLSDNGSAYRSHAWRDFCARLGIRHKRTRPTGRRRTGRSSDSTHARGRLGLCQVLRFRGRTTLALPGWL
HFYNHHRHHSAIGGVPFDRLNNVPGHHI
STVHAVLVRCRVNRLSHIDRVTGEPLRRYEHPHPGSLIHVDVTKFGNIPDGGGHRYVGRQQGARNKLATPGLPRGKDHKPRTGTAFVHTVIDDHSRVAYA
EIWSDEQASTAVGVLERAVAWFAERGVTVERVLSDNGSAYRSHAWRDFCARLGIRHKRTRPTGRRRTGRSSDSTHARGRLGLCQVLRFRGRTTLALPGWL
HFYNHHRHHSAIGGVPFDRLNNVPGHHI
Blast result :
Comments
The ORF1 of ISCgl1 encodes the chloramphenicol resistance gene. ORF2 is a transposase which is 91% aa similar to IS5564, 57% to IS1649 and ISAni1, 55% to ISSco1, 51% to IS1121, IS1122 and ISBm3.
This is a transposon-like sequence.
There is no stop codon at the end of the element, the transposase use the first Stop codon after the end of the IRR. The transposase in the database is cut at the end of the IS.
This is a transposon-like sequence.
There is no stop codon at the end of the element, the transposase use the first Stop codon after the end of the IRR. The transposase in the database is cut at the end of the IS.
References
1] Shi,S. (1997) Direct Submission GenBank