ISfinder Mirror Site

ISCco1

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Campylobacter coli	Campylobacter coli RM2228

DNA section

IS Length : 1871 bp

Ends

Left end : CAATTTCCAAGCAAGAAAACTCTCGCTTCTATAAGCGAGAGATGAATTGCTTTTTTTCTAAAAATTATAACATATTTCAGTAAAATTTGCTATAATTATC II struct. : Yes
Right end : AACTAGCCTATTAGACTATAAACAAGAAGCCTTGCGTGTTAGCTGACATTGGTTGGCTTTAGTAAGGAAGCACCTACTTCTATAAGTAGGTGTGGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TTGATGGCATTTAATT	TTAT	CAAGAGATTTGATTTACTT	TCAC
AGAATATTTTTTTATT	TTGT	TTAATTCTTTAGATAAAAA	TCAC
ATCTTCTTTTTTATTT	TTAT	TTTTAAATCTTTTATATTA	TCAC
AATGGGGAACTAAAAT	TTAT	TTTAACTTGGTTTTGAAGG	TCAC
TTTATATGCTAGTTTT	TTAT	GTATTAAAAATTTGTTAAT	TCAC

DNA sequence

CAATTTCCAAGCAAGAAAACTCTCGCTTCTATAAGCGAGAGATGAATTGCTTTTTTTCTAAAAATTATAACATATTTCAGTAAAATTTGCTATAATTATC
TTATGAAAATAGAATATGAGAGAAATTTACATTCAGTATTTTTGTTACAATATCACTTAATATTAGTAGTGAAGTATCGTAAAAAGGTAATTACTGATGA
GATATGTTGTAGATTAAAAGAGATTTTTGAGTATATTGCTAAATCTAGTAATTATCAATTAGAAATAATCGAGTTTAACCACGATATTGACCATTTGCAT
ATCTTATTTAAGGCAAAGCCTAAAAGTGAACTACTAAAATTCATAAATGCTTATAAGTCTGCTAGTAGTAGACTTATAAAAAAAGAATTTAGTGAAATTA
AAAAATATCTTTGGAAAGAATATTTTTGGAGTGGTAGTTATTTTTTAGCTACCACAGGTGGAGTTAGCTTAGATGTTCTAAAAGCATATGTGCAAAATCA
AGGTATAGAAGATAATAGAGTAAAAAAGCAATATAACTCTAAACACAAACGACTGAAAGAGCAAGAACTATGCAAATAACCAAAGGCTTTAAGTATAGGC
TATATCCAAACAAAGAGCAAAGCTTAAAGCTTTCTCATCAATGTTTCATCTATAATCAAGCCTATAATATTTGCCTAAACTTACAACAAGAACAATGGCT
AAAAAATAAAGAATTAGATAAAAAAGAAAGAACTTATTTAAGTGCTACTCAAATCGATAATCAAGTAAAACTAGCCTTAAAAGCTAGAAACTTAGAGTTT
AAAAGTGTAGTAGCACAACAAGCTAGAATAAATGCTAATAGAGCATTAGAACAAGCAATAAAAGATAAAAATAAAGGTTTTCCAAAGTTTAAAAACTCTA
AATTGGCAAAGCAAAGTTTCAATTGGAATAATCAAGGCTATCAAATTCTAGAATTTAACGATAAGTTTAAAATATTTAGACTTATGTCTATGCCTATAAA
AATGCGTTATCATAGAGCTTTGCCAAATAATTATAAACTAAATCAAATAACTATTTCTAAAACTCATAATAAATACTTTGTCTCATTTAGTATAACTTAT
GATGAACCAAGTGTTGTTTTAATGTCTAGCAATAAAATAGACATTAACAGAGCAGTTGGCATTGATATGAATATCAATGAAATTGCTTTAAGCAATGGTG
CTTTGTTACTGACTTGTTCTAAGAGTATAAATAAAGCTAAATACTCTACTACTTTCAAACGCTTAATGCGAAAACAAAGTAAAAGAGTATTGAAAGCTAA
AAAGGATAAGATTAAGTTAGGTTCTAATTTTAGAAAAACTCAAATTAGACTTAATAAAATCTATGAAAGAGCTAGTAATATAAAAAAAGATTTAGCTCAT
AAGATAAGTAATCAAATAGTAAAAAACTTTGATTTGATAGTAGTAGAAAATCTAAAAGTGAAAAATATGACTAAACGAGCAAAGCTTAAAAATGTAAAAG
CTAAAAGTGGCTTAAATAAGTCTATATTAGATACTTCTTTCTATCAATTTTTAAGCTTTGTGGAATATAAAGCTATGCATAATGGCAAAGTATTTACTAA
GATAGAACCACAATATACAAGCAAAGCTTGTTGCAAATGTGGAACTATAAAAGAGAATTTAACTCTAAAAGATAGAGTTTTTACTTGCGATAATTGTGGA
TATGTAGAACATAGAGATATAAATGCAAGTAAAAATATCTTGGATAGAGGCTTGAAGTCGTTTGGGCTAGGAACTAGCCTATTAGACTATAAACAAGAAG
CCTTGCGTGTTAGCTGACATTGGTTGGCTTTAGTAAGGAAGCACCTACTTCTATAAGTAGGTGTGGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
477 bp	158 aa	103	579	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKIEYERNLHSVFLLQYHLILVVKYRKKVITDEICCRLKEIFEYIAKSSNYQLEIIEFNHDIDHLHILFKAKPKSELLKFINAYKSASSRLIKKEFSEIK
KYLWKEYFWSGSYFLATTGGVSLDVLKAYVQNQGIEDNRVKKQYNSKHKRLKEQELCK

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1248 bp	415 aa	570	1817	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MQITKGFKYRLYPNKEQSLKLSHQCFIYNQAYNICLNLQQEQWLKNKELDKKERTYLSATQIDNQVKLALKARNLEFKSVVAQQARINANRALEQAIKDK
NKGFPKFKNSKLAKQSFNWNNQGYQILEFNDKFKIFRLMSMPIKMRYHRALPNNYKLNQITISKTHNKYFVSFSITYDEPSVVLMSSNKIDINRAVGIDM
NINEIALSNGALLLTCSKSINKAKYSTTFKRLMRKQSKRVLKAKKDKIKLGSNFRKTQIRLNKIYERASNIKKDLAHKISNQIVKNFDLIVVENLKVKNM
TKRAKLKNVKAKSGLNKSILDTSFYQFLSFVEYKAMHNGKVFTKIEPQYTSKACCKCGTIKENLTLKDRVFTCDNCGYVEHRDINASKNILDRGLKSFGL
GTSLLDYKQEALRVS

Blast result :

Comments

ISCco1 is 78% (ORFA, the transposase) aa similar to ISEfa4 and 66% (ORFB) to IS606.

References

1] ISfinder annotation (2010)
2] Fouts,D.E., Mongodin,E.F., Mandrell,R.E., Miller,W.G., Rasko,D.A., Jacques,R.J., Brinkac,L.M., DeBoy,R.T., Parker,C.T., Daugherty,S.C., Dodson,R.J., Durkin,A.S., Madupu,R.R., Sullivan,S.A., Shetty,J.U., Ayodeji,M.A., Shvartsbeyn,A.A., Schatz,M.C., Badger,J.H., Fraser,C.M. and Nelson,K.E. (2004) Direct submission GenBank.