ISfinder Mirror Site

ISCausp2

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010338	ND	Caulobacter sp.	Caulobacter sp. K31

DNA section

IS Length : 7915 bp

Ends

IR Length : 25/26

IRL : CGCCATTGTGGAGCAAACGCACCAAGTGGCCTGAGACCTAAGTTATTGTT
IRR : CGCCATTGTGGAGCAAACGCACCTAGGCACTGAATCGGGGGTCCGGGGGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCGGCGAACGAGCG	GCTTTCGG	CGCCGTAGAGGGTG	8

DNA sequence

CGCCATTGTGGAGCAAACGCACCAAGTGGCCTGAGACCTAAGTTATTGTTCTTGCTACGTTCTAAGCATGAGCAACGATGTCATTCCCTGGGATGCCGAC
CAACCCAACGTCCTGCACGCCCTTACAAGGAAGCGTGCCGAGATCGCCGGCCAGATCGAGCACAACCAACTCACCCTCCGTCATCTGATCGCCGAACTCG
ACCACGTCGATGCGACGATCCGCATCTTCAACCCGGCCGTCGATATTGGCGCGATCCGATCAAAGCCGGTTCCGCCGCGCCATGCGGCGTTCAAGGGTGA
AGTGACGCGGGTCGTCTTCAAGACGCTTCGGGAAGCGACGGCGCCGGTCACGTCTCGCGACATAGCAATACGCCTGATGAACGAGCGGGGCCTGAACCCA
GACGACAAGGAGTTGTCCGTCATCATGGTCAAACGCGTGTGCGCCTGCCTGCGAGTGCAGAAGAACAAGGGCTTGATCCGCAACGCTGAGAGCTTCGGCA
ATCTGCAAGGCTGGGAGGCTGTGCGATGACACCCTTTCGTACTGGCGCGCTCCGGTTCACCCGTGGGCGCCACGCCTACATCGTCGCGCCGGCGTATGAC
GGCGACGGCTACATCGGGCTCCGAGATGGACGGATCATAGCTAGGGCATCGGAGCGTGCGGATGTGACCCGCGCGCTGATCGTTCGGTATAACCTGTAGA
GCTTCTTGGTCGCGATCTTCGCTCCACACCGAATACTGCGGAGGCTGTGCAGTCGTCTGGGGTGCGAGCCCGGCCGCATCGGACTGGTCAGCGGCGCCCG
AATTTCTTAGAGCTTGGCCGTTATCCCTGGATTGAAATCTGCTATTCTCGCTGAGTAGTAGAGGGACGCTCCATGCCATCGACGACCGATTTCCAAGAAT
TTCTCGACAGCGAAGCCCCAGATGGCGCTCAAGAGCGCGCCGATCTGGCCGCCTGCGCCTCTGGCCCAGACGATATAGGAAGGTATTCTAGCGTAATTGC
TCCGAACGGCGCCCTGATCGTCAGCGGTGGTGGGGAGACTACGTTAGCGCTTACCTCTCCGAAGGCCAAGGCGGCGTTCTTGGCGATACTTGAAGGTGAA
GTTAGCGAGCCTGGAGCGACCCTGGAGGGCGACGCTGAATTCGAACGGTCCATGCAGAAAGACGACTAGGCGTCGCCGAAGTTCGAACAGGTGCCTAATC
GTCGAGGACGGGAATGCGATGGAAGCAGACTTCCGGTGCCTCCCATTCCTCCACTAACCCATTAGCGTTTCGCCACGCGGTAGTAATCAAGCCCATCCCA
TCGATGTCTACTACGAGCATTTTTTCACTACCGCTGTTCCAGCGGACAACGTCGCCGATGCGGATCGCAGGCTCTCGCTGATCACGGAAATCTTCAGCGC
GCTTAACGAGGCGTAAGTCGTTTTCGTTGTGTCTTTGGGTGTGAAACATCGGGGATTGTATAACCTGTGAGTGAGCCAACCTTCAAAGCCGGCGATACGG
TGCGCCTAAAGTCCGACACGGTCCTAATGACAGTGGAAAGGCTCTTCCCAACAGAAGACGACAGTTGGGAGTATGTCTGCGTATGGCGAGCAGACAACAA
GACGCTTCGAGAGCGTTATGCGGAAGTTTTACTTGAGGCGGCAAAAAGGCCGAGGCACCGATCCGTGCCTATACGCAGGGGCTAGTCGCAAGCGTCGAAA
CTGAAGCCTTATAGGAAAAGAATCCTATAAGGCTTTGGGCTTAAAGTCAAGAAACGCGTCATAGTAGGTCGCCCGCCGGCGAGTGGCGGCCATTTTTATG
CTGACGAGGGGCTATATACCTGGGCGGGTTGCTGCCTGAATCAGGTATTTTTTCACAGTATCATCTAACGCCAGCACCGTCGTGTAGCGCAGTGTCACCG
TTTCGCCCGTTCCATTGGCATACCTATCGAGTCCCCAAGACATAATTGGCTTTACTCGCCTAAGAACGTCGGCCTTCATAGCGAACGGAACAACACGGCC
GATTTCATCATCGAACACTCCTCCGTGTCCAGAGTTTACGTTAAAAGACGCAACGGAAAAGTCGCGCATTTTAACTGTATTATCAAAATAGAATCCTTCG
ACATAATGCTTGGTTACCGGAGTAAAGCTACCAATCTGGTGTGTTCCTCCGAAGATGTTCATCACCTTAGCGCCATCGCCAATTACCGAATGCGCCTGGC
GAACCGATGGTTCTGTCGCCGCAACGAGAGCCTCCAGATCACCTCCACGCGTGCGTTCAAGGTGCCGAAGCTCGGCGGTCTGGGGCTGGGGCTCGATGCC
CAAGTTTCGATCGATTGTTCTTTTGAACGTGTCGTAGAGCAGGGCCTTGCCAGCCTCAGGCGCGATCTGCACCGACGTGCCGAGCGCTGAAATTCCGTCC
AGATAGACGCGGAACTTCGAAAGAACGCTCCCCTCTTCGAGGGCAAAAGCCTTTACCGCAGACCGGCCGGTGAAATCGCCTCGCCGTCGAATTTTACCTG
TTTCAATGTAGTTGGCGACCAGGGATAGCGATAGAGAAAAGCCAGCCAGAGAAGTGTAGCCGTCATACGCGGGCATCGCGTGAAATGAGGCGTCGCCACC
ATCTAGTTCTAATTGGAAAGGAACTTCTACCAGCACCGTGGGCGCCCCATCTCAACCAAGCAAGCTTCACTGAAGCCGCTCCACGGACGACATTGCAACT
GAAATTTGCCCGTCAGACGATAACGACGGGCACGCTCTCAACTTCCTGAGATGAAAATTCCAAGCGCAGGACGAGTTTCAGCACGCGCCAATGATCACCG
GAGGTCCAGTTAATCACCCGCATTCGCGGCCCCTTGAACTCGAACTCGTGCATGTGAAGCCGCACCACATGCCTATCTTGGATGCCTTTTTCATAGGGAG
AGTCATGCGTGAACATAAGTTCGAATGCCGGAACCTCTTCTGGCCGAACGGGAAATCCTTCTCTAACCTGAGATAGCAAGCCGCACCAATGTATGAACGC
CGAGTTCAACATCGTAACTGGAAGAAATATTCTATCTTCCTGAGATGGCCCCATGTCACCGAGAAATACGGTGTCGGAACTACCAACGATCATCCTGTGC
TTTATGGTATCGTTGATACTAAATTCCACTAAGTACCCATCAGCAGCAAGATTAATTGACCTATCTGCTCCGCACATATCTCGAACGCGGTCAAAGACGT
GATCAGGTAATGGAGTTATAGACTTCCAATTTATACAGATATTTTTTGCGGCTCCCTGAGACGCGTTTCTTGCCACTATTGGGAATGGAATGCTTAGTGG
GGGCGCATTGTCTGGATATAAACTCCACGTAAAATCAATTATGTTCGAAGGAGTTTTGGGGGTGATGCGAGCCCTAGCCCCCAGAACTCTTGCTGCGCGC
ATCTCCTTTACCGTCAAGTAGGTTGCAAATGCCGCGCCCAGCGATCCGAGCGAGGCAACGGCAGCTACGATCTTGTCCCACGAGGCTGAGGTGGTGGTGC
TTATAATCCCCCCCAGGATACCGGCAGCCAATCCGCAGCATGCAACCAGAATCCAAAGGTCCCAGCGAGGATGCTCGCCCGCCGGCCCAGGATGACTCTC
TCGTTTTTTGTCAGGCAACATCTATGAACCCCTGAGCAGAGTCTCCCATCAACCCTACGTCGAGGCAACTCGATGGCGCTCATTTGATTTGCCGGTCGTA
TTCCTAAGCGCCGATCAGCGGCGCGGCGGACGACTGATCGCAGCCGAACGACGAGTAGGTGTTCTCGTCGACGTTCAGATACAGCTCCGGCGTCGTGCCT
TTCACGCCGCCCGTGTTGTAGACGAACAAGATCGGCTCGCTGGCACCCATGTAGCCACCGCAGACCGTTGTATCACCGTCCTTCTCTTTATACGCCCAGG
CCCTCGACACCGAGACCACCCGGCCAACCTTGGCCGAGATCAGCTTCTCCAGCCGGCCCAGCCAGATCGACTTCTCCTCGTTGTTGACCGTGATGGTCTG
CCTCAGGCCAACGGGAGGAATGTAGTGGACGTCGTCAAAAGTGGTGTGCTGCTCAGGATCGACACGCGCCGGCACCGGCCCCCCGGCGGTGATGTCACTG
GCCGACGATGTGATAATCGGCTTGGCGACCGAAGCTGCCTGCCTGGTCGCGGGAGCTGGTGCGGTCGGTGAATTGATCACCTTTGGCGCCGGCACCGGAC
GCAGCACCCAAACTGCCGCCAGGAAAACGCCGGCGAAGATCGACGTCACCAATATCGCGAACCGCATTTCCCGCCCCCAAAAACCAATATCATCACTATA
GTGACTGGCTCTAGGTTGAGGATTCGTTGCCGCTGCCCCCTATGCGTTTGGGGCAGGCCCTCGGGATAAATGTGCGCCGGCTTCGTAAGGAGCGCGGGCT
GAGCCAGGAGGCGTTGGCCGACGCCGTCGGGCTTGCGCCGACCTATGTCGGGCAGATCGAGCGCGGCCTCAGAAACCCGACCCTGGATGTCGTGGAGCGC
TTTGCGGACGTTCTAGGCGCGGAGGCGCTGGACCTTCTGCGAGTTAGCCCTGACCCGAGATGAGCTTTTGGCGGATCACTTCTGCGTCCGGCGCATCTGC
ATCGAACGGCAGATCGTAAGCCATCAGGTCGACCGTTCGGACTCCCAGGGCCGCAAGACATTGAATCAGAAACACCCCGGAGAAGGTGCCTCTGGCCACC
TTGTTGCGGATATTGCGCTCGTTGTCGTCAACACCCAGGGGCCTCAGGCGCTCGACCATGTCGGCGTAAGTAAGTCCCTTTCGCACCATCTCTATACGGA
CTATCCGGTGCGCAACGTCGTTCCAGGCGGCCACGCGCATCTCCTATGGAGCTATAGCATCATATATGATGCTTTTTGATTGACAAACGCGCCCTGCGTC
GTGCAAAGGCATCATATATGATGCTTATGCACGGAAAGGCAGTTTGCGACAACACTTTCTCCTCAGCGCTAAGGCGCGGACGCTAGGCGTCGTCGAGATC
GCTCGTATGAGCGATGACGAAGCTTACGCCGCGTTCCGGGCGGTTCGCTTCGCGGACAACGGAGGAGAGGCCTACTGCGCTCACTGCGGGTGCGATGCCG
TTTACGAGTACGCGGCGCGGCGCATTTTCAAATGTAAGGCGTGCGAGAAGCAGTTCTCGCTAACATCGGGCACGATTTTCGCCAGCCGGAAGCTGGCGAT
CCGCGACATCCTGACGGCGATCGCGCTGTTCGTGAACGGTGCCAACGGCCACGCTGCGCTGCGGATGGGCCGCGACCTCAATGTCTCGTACAAGACCGCG
TTCGTCCTACTGCATAAGCTGCGCGAGGTGATGGGGTCGCTTAAGCCAAAGGAAATGCTCACCGGGCAAGTCGAAATTGACGGTGTATGGGTTGGCGGCC
ACGTCAAAGGACCAAACCTCAAAATCGAGCAGAAAGAGCGGCGCCGGAAGAAGGCGGGGGGCGAGCCGGAATATCCACCCAAAAATGAGGCTAAGCGCTT
CTCGGTCGTAACCATGCGGGAACGTCGCGAAGGGGGCCGCTCACTATCCTTCGTGTTTAAGAGCGAAAGCCAGGGCGTCAAATCCGTCCTGGAGAACGTT
GATCCAGCCGCTGAGGTGATCACGGACGGTGGGCCGCATTGGGGGCAGCTTGTGCTTCGGTATGCCAATACCAGCCAGGTGAACCATTCCATTGGCCACG
TTATCGATGGCGTCCATATCAACGGTGTGGAGGGCTTCAACTCGCGAATTCGACGTGGGGAGCGTGGCGTCCACCACCACATCGCCGGCCGGTATCTCCA
GTTCTACGCTGACGAGTTTGCATGGCGCGACGACCATCGCCGCGTCAGCAACGGTCAACAGTTTCAAATGGTCTTGGCGGCGGCAGCGCGGCACCCGGTG
TCGGGCGAATGGAAAGGTTTCTGGCAGCGGCGGAAAGCCGGCGCTCCGGCACGGGCACCCGCGTCCAGCGCATCGGCGGGCCGTCGAGGCGAACGACCAC
CCCCTCGTTAAAGCGCGCCTGAAAAGTGTTTTGTACGGACGAGCTGATCTTAGCCAGTGCTCGATGATCGCTAGGATCGACGCCGATCTCGACCGCGACT
ATTCGGGCGACCTCTGAGCTGGTCAGCGGCTGATCGGCGGTCCGCAGCGTCGCATAGGCAAGGCGTGAAATTTGTCCCGTCGGCTTCTTACCCTGTCGGA
AATAGATCGGCTTGATGTGGCCAGCGGTCCAATCCGGCACGTACCGCTGCCGAATTTTTGCAGCCGTCTCCTCCATCACATCTAGATACCGCTTCCGCCG
TTCGACGACGCGGGCGGCGGACAGTTCCAGCGCTTTCCGACCAACGACAACTTCTTGGACCTGCGCCGCCGTCTCGTAGTTCCCCAGCGCGCGGGCGTAT
CGCCGCTCCAGCGTCTCGATCGTAGCTTGGTTTCTCATGGGCGCATCCCGCCCGTGTATTCGTAAAAATCCCCCTACAACTTTAACTTAAGCGCCCCGAT
GAGTTGTGAAATCGATTCGACTCGGGGTCAGGAATCAGCCGACGTTTCGCTGCTCGTCGGGAGCGTCGGGAGAAATCTGATGGCAGACAAAGGTGATAAC
CTCCGTATCAAGTACGGGTTGGTCAACAATCCGAGCATCGCGCTTCAAAAGCAGTGGGCGGCTCTCACCGAGGCGCTGATCCGCCAAGGTAACACTAGCG
ACGCCGCTGGCCGACTAGCGGCGCAGCAGTTGTTCCCCGAATATAGGCAAAGAGTCTACGCCTCCGAAGGAGACGCGATCGAAATGCTGTTGCGTCAGAT
CAACGACAAATGACGCAGGCGATACCGCTCTGGCAGGCGATGGTTTCGCTATTCGGCGTAATTGTCGTCGGCCTGATCGGCCATATGGTTTCGGTTGCAA
AGCTGAAGACCGAACTTGACCAAAAGAATTTCGAAAACTCAATGAGAGTTCTCGAAACCCATGATGCTGCCTACAGGACTTACACCTCTGCAATGGAAGC
TTATGTTTTAGCTCCTGAGCCTGACTACGAAGATTTTATGAAAGTCGTCTCTTCTGGCGATGTGTATTTTAATCAGCTAAATCTGATTTGCAGCACCATG
ATTTCAGGCAAAGTAGATCACAATATCCGAGATAAAATCTGGATGCCGAAGATCAAAGTGGCCTTCGAAAAATCCCTGCCCATGCACTATGACACTCTGC
GCAATGCAGCGAAGAAGAGGGGCTTCCCCTACAAGGGCGAGCTTCGCCGGCGGGATCACGAAAGCATTTTCGCAGTAGCGGAAATGTTCAGCGCATCCGA
TGCATGGCAGCGCCCACACGAAGCCAACTAGCTCCTCTTCCATACCGTCTTTGTCGAGAAGGAAGAGTGATGCACGCTATCGCCACCCCGCCGTCAACGG
TCTTCAACTGGGATGCGCTTTCAGCACTCGCTACGCTTGCCGCGACCTTGGCAGCGATAGGGATCGCCCTAGCCACTCATATGATAGATAGCCGTCGGCG
CCGCGCAGCATCACTGAGTGCGCTGAGCTTGGCGGGCAAGATTGCGAAGGACGCAATCTCATTTTTTGAAGCCATCGCCTTGCAAGAAACTGGTTCGACG
TCGAGCCACCGAGCAAAGTTCATTGTTGGCGGTGCCCCGATGCAGGAGCTTTTCCAGAACATGGGAGAGGTCCGCCTGATGGATTTGCCAACATCATCTG
CGATCGACGCCTACAGCCGCGTGCGCTCCCACGTGAGAAGCTACGAGAAAAAGCTGGAGGTGTTGATCAACACGCCTGGTTCCGCCGGAAGGGTCGGCAT
CTCCGAACACCTTCAAGGCTGCATCGCGGCGGCAGCGATCATCGAGGCGGAACGGAAGCGATAATACCCCCGGACCCCCGATTCAGTGCCTAGGTGCGTT
TGCTCCACAATGGCG

Protein section

ORF number : 10

ORF 5

Length		Begin	End	Strand	Fusion ORF
192 bp	63 aa	4563	4372	-	No

ORF function : Passenger Gene
Annotation : Transcriptional regulator, XRE familyDescription : Transcriptional Regulator factor

ORF sequence :

MRRLRKERGLSQEALADAVGLAPTYVGQIERGLRNPTLDVVERFADVLGAEALDLLRVSPDPR

Blast result :

ORF 1

Length		Begin	End	Strand	Fusion ORF
462 bp	153 aa	68	529	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSNDVIPWDADQPNVLHALTRKRAEIAGQIEHNQLTLRHLIAELDHVDATIRIFNPAVDIGAIRSKPVPPRHAAFKGEVTRVVFKTLREATAPVTSRDIA
IRLMNERGLNPDDKELSVIMVKRVCACLRVQKNKGLIRNAESFGNLQGWEAVR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
297 bp	98 aa	873	1169	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPSTTDFQEFLDSEAPDGAQERADLAACASGPDDIGRYSSVIAPNGALIVSGGGETTLALTSPKAKAAFLAILEGEVSEPGATLEGDAEFERSMQKDD

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
825 bp	274 aa	2636	1812	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MLVEVPFQLELDGGDASFHAMPAYDGYTSLAGFSLSLSLVANYIETGKIRRRGDFTGRSAVKAFALEEGSVLSKFRVYLDGISALGTSVQIAPEAGKALL
YDTFKRTIDRNLGIEPQPQTAELRHLERTRGGDLEALVAATEPSVRQAHSVIGDGAKVMNIFGGTHQIGSFTPVTKHYVEGFYFDNTVKMRDFSVASFNV
NSGHGGVFDDEIGRVVPFAMKADVLRRVKPIMSWGLDRYANGTGETVTLRYTTVLALDDTVKKYLIQAATRPGI

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
564 bp	187 aa	4267	3704	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRFAILVTSIFAGVFLAAVWVLRPVPAPKVINSPTAPAPATRQAASVAKPIITSSASDITAGGPVPARVDPEQHTTFDDVHYIPPVGLRQTITVNNEEKS
IWLGRLEKLISAKVGRVVSVSRAWAYKEKDGDTTVCGGYMGASEPILFVYNTGGVKGTTPELYLNVDENTYSSFGCDQSSAAPLIGA

Blast result :

ORF 6

Length		Begin	End	Strand	Fusion ORF
297 bp	98 aa	4840	4544	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRVAAWNDVAHRIVRIEMVRKGLTYADMVERLRPLGVDDNERNIRNKVARGTFSGVFLIQCLAALGVRTVDLMAYDLPFDADAPDAEVIRQKLISGQG

Blast result :

ORF 7

Length		Begin	End	Strand	Fusion ORF
1068 bp	355 aa	4944	6011	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRQHFLLSAKARTLGVVEIARMSDDEAYAAFRAVRFADNGGEAYCAHCGCDAVYEYAARRIFKCKACEKQFSLTSGTIFASRKLAIRDILTAIALFVNGA
NGHAALRMGRDLNVSYKTAFVLLHKLREVMGSLKPKEMLTGQVEIDGVWVGGHVKGPNLKIEQKERRRKKAGGEPEYPPKNEAKRFSVVTMRERREGGRS
LSFVFKSESQGVKSVLENVDPAAEVITDGGPHWGQLVLRYANTSQVNHSIGHVIDGVHINGVEGFNSRIRRGERGVHHHIAGRYLQFYADEFAWRDDHRR
VSNGQQFQMVLAAAARHPVSGEWKGFWQRRKAGAPARAPASSASAGRRGERPPPR

Blast result :

ORF 8

Length		Begin	End	Strand	Fusion ORF
315 bp	104 aa	6499	6813	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSCEIDSTRGQESADVSLLVGSVGRNLMADKGDNLRIKYGLVNNPSIALQKQWAALTEALIRQGNTSDAAGRLAAQQLFPEYRQRVYASEGDAIEMLLRQ
INDK

Blast result :

ORF 9

Length		Begin	End	Strand	Fusion ORF
522 bp	173 aa	6810	7331	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTQAIPLWQAMVSLFGVIVVGLIGHMVSVAKLKTELDQKNFENSMRVLETHDAAYRTYTSAMEAYVLAPEPDYEDFMKVVSSGDVYFNQLNLICSTMISG
KVDHNIRDKIWMPKIKVAFEKSLPMHYDTLRNAAKKRGFPYKGELRRRDHESIFAVAEMFSASDAWQRPHEAN

Blast result :

ORF 10

Length		Begin	End	Strand	Fusion ORF
561 bp	186 aa	7304	7864	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAAPTRSQLAPLPYRLCREGRVMHAIATPPSTVFNWDALSALATLAATLAAIGIALATHMIDSRRRRAASLSALSLAGKIAKDAISFFEAIALQETGSTS
SHRAKFIVGGAPMQELFQNMGEVRLMDLPTSSAIDAYSRVRSHVRSYEKKLEVLINTPGSAGRVGISEHLQGCIAAAAIIEAERKR

Blast result :

Comments

ISCausp2 is 65% aa similar to ISNwi4. The transposase is the 7th ORF, others are passenger genes.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Bruce,D., Goodwin,L., Thompson,L.S., Brettin,T., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stephens,C. and Richardson,P. (2008) Direct submission GenBank.