ISCac2
- Family IS1595
- Group ISPna2
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_003030 | ND | Clostridium acetobutylicum | Clostridium acetobutylicum ATCC 824 |
DNA section
IS Length : 1838 bp
Ends
IR Length : 21/25
IRL : GGCTATGTTTTAAATCAGTGTTGAAAATAGATATATATTAGAAAAATAGG
IRR : GCTATGTTTTAATATAGTATTGAAATATAGCCTAAAAAAATTATAGGTAG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GGCAAGTTTGCTCTT | TTTTATTTT | AAATAAACCGTTATATA | 9 |
DNA sequence
GGCTATGTTTTAAATCAGTGTTGAAAATAGATATATATTAGAAAAATAGGTTATAATATTAGATAGAATAGTGTTTAGGAGGTAGAATTATGCCAAATGT
GAAGTCTATATTAAATCATATTGAAAAGTTACCATTACAGCAGAAAGAACAAATACTATCAACTTTAGAAGAAATTCTTGTCCTAGGTTCACAAGTAAGC
CAAGTAGAACAAGAAGTTAAAGAATTTAGATTTTCCAAGGGGAAAGTATGCCCAATTTGTGGTTCTGAAACTATATCAAGAAATAGCAAGTATAATGGTA
AACAAGGATATATCTGCAAGTCTTGTAAAAAGTCCTTTACTGACTTCACTAATTCTGCAACCTATAAAAGTAAGAAAACATTAGATAAGTGGCTTAAATA
TGCTAAGTGCATGGTTAATGGATATTCTATCAGGAAGAGTGCTAAAGTTGTAGAAATTAATATAGCAACAAGTTTCTTTTGGAGACATAAAATTCTTGAT
TGTGTAAGCACATTTTAGGCAAGGGGTATGTTGATGGTGTTATTGAAGCAGATGAAGTATTCTTTACTGAAAGTTTTAAAGGTACAAAACCTTCTAATAT
GCCAAGACGCTCTCGTAAAATCGGCAAACAAGTTAAGAAAAGTGGTATATCAAAGGAACAAGTCTGTATTACTACTGCTATTGATAGACAAGGTAATTTA
ATTATGGAATTAGCATGTAAAGGTAGAATTACCTCTAAGGAATTAGAAAAATTATATGATGGACATATAAGCAATGAATCTATACTTTGTACTGATAGTC
GTAAAAGTTATATACAATTTGCAAATGATTTATCATTAGAACACAAACGTATAAAAAGAGGTAAACATAAGGAAGGACTATATCATATTCAACATATTAA
TGTTCTACACAGTAATTTAAGGAAATGGATGAATAGGTTTAATGGTGCTGCAACAAAATATATTAGTAACTATATTAAGTGGTTCAAATGGCTTCAAATA
TTTGATACACATAAAGAAATAATAAAGGCTAAAAACTTTATAGTTCAAAGCAATGTAGCACATGCATATATCAAAGTAAAAGACTTAAAATATAGAGAGC
CAATATGTGTATAACATATGTAATATTTTGTAAATGATGCAATAATAAGTGCTATTAACAACTATAATTAGATTGAATTTTATAAATATGGAGGTTTATA
GATGTACAACAATAGAAAATTAGTTCTATATATTGCAACAAGTTTAGATGGTTACATAGCGACAGAAGATGATTCCTTGGAATGGTTGTTTAAAACTGAA
GGCGAAGGAGATAATGGATATTTTGAATTCTACAATACAGTTGATACTATTCTAATTGGTAGAAGAACTTATGATTGGATTATTGAAAAAGAAAAAGGTA
AATTTCCTTATAAGAATAAAAAGTGTTATGTATTTTCTAAATCAGAAAAAGGTAAAAATGAAAATGTAGAATTTATTAATCAAGATGTAGTGGAATTTAC
AAATAAAATCAAAAGACTGGATGGAGGAAACATATGGCTTGTTGGCGGTGGTGTTCTTCTAAATTCTTTTATTAAAGAAAGGTTAGTTGACGAATTTATT
ATTACTATATCACCTACACTAATAGGTCATGGAATACCTTTATTTAAAAAAAATGATTTTGAGTTAGAACTAAAGTTAAAGGGGATAAGGAGATTTAATC
AATTTGTTGAACTCCATTACGAGGTGAAATAAAACAATTGATATTTAGTTCATAAATTTCTTATAGTGAGTATTATAGGTGGACAATCCTACCTATAATT
TTTTTAGGCTATATTTCAATACTATATTAAAACATAGC
GAAGTCTATATTAAATCATATTGAAAAGTTACCATTACAGCAGAAAGAACAAATACTATCAACTTTAGAAGAAATTCTTGTCCTAGGTTCACAAGTAAGC
CAAGTAGAACAAGAAGTTAAAGAATTTAGATTTTCCAAGGGGAAAGTATGCCCAATTTGTGGTTCTGAAACTATATCAAGAAATAGCAAGTATAATGGTA
AACAAGGATATATCTGCAAGTCTTGTAAAAAGTCCTTTACTGACTTCACTAATTCTGCAACCTATAAAAGTAAGAAAACATTAGATAAGTGGCTTAAATA
TGCTAAGTGCATGGTTAATGGATATTCTATCAGGAAGAGTGCTAAAGTTGTAGAAATTAATATAGCAACAAGTTTCTTTTGGAGACATAAAATTCTTGAT
TGTGTAAGCACATTTTAGGCAAGGGGTATGTTGATGGTGTTATTGAAGCAGATGAAGTATTCTTTACTGAAAGTTTTAAAGGTACAAAACCTTCTAATAT
GCCAAGACGCTCTCGTAAAATCGGCAAACAAGTTAAGAAAAGTGGTATATCAAAGGAACAAGTCTGTATTACTACTGCTATTGATAGACAAGGTAATTTA
ATTATGGAATTAGCATGTAAAGGTAGAATTACCTCTAAGGAATTAGAAAAATTATATGATGGACATATAAGCAATGAATCTATACTTTGTACTGATAGTC
GTAAAAGTTATATACAATTTGCAAATGATTTATCATTAGAACACAAACGTATAAAAAGAGGTAAACATAAGGAAGGACTATATCATATTCAACATATTAA
TGTTCTACACAGTAATTTAAGGAAATGGATGAATAGGTTTAATGGTGCTGCAACAAAATATATTAGTAACTATATTAAGTGGTTCAAATGGCTTCAAATA
TTTGATACACATAAAGAAATAATAAAGGCTAAAAACTTTATAGTTCAAAGCAATGTAGCACATGCATATATCAAAGTAAAAGACTTAAAATATAGAGAGC
CAATATGTGTATAACATATGTAATATTTTGTAAATGATGCAATAATAAGTGCTATTAACAACTATAATTAGATTGAATTTTATAAATATGGAGGTTTATA
GATGTACAACAATAGAAAATTAGTTCTATATATTGCAACAAGTTTAGATGGTTACATAGCGACAGAAGATGATTCCTTGGAATGGTTGTTTAAAACTGAA
GGCGAAGGAGATAATGGATATTTTGAATTCTACAATACAGTTGATACTATTCTAATTGGTAGAAGAACTTATGATTGGATTATTGAAAAAGAAAAAGGTA
AATTTCCTTATAAGAATAAAAAGTGTTATGTATTTTCTAAATCAGAAAAAGGTAAAAATGAAAATGTAGAATTTATTAATCAAGATGTAGTGGAATTTAC
AAATAAAATCAAAAGACTGGATGGAGGAAACATATGGCTTGTTGGCGGTGGTGTTCTTCTAAATTCTTTTATTAAAGAAAGGTTAGTTGACGAATTTATT
ATTACTATATCACCTACACTAATAGGTCATGGAATACCTTTATTTAAAAAAAATGATTTTGAGTTAGAACTAAAGTTAAAGGGGATAAGGAGATTTAATC
AATTTGTTGAACTCCATTACGAGGTGAAATAAAACAATTGATATTTAGTTCATAAATTTCTTATAGTGAGTATTATAGGTGGACAATCCTACCTATAATT
TTTTTAGGCTATATTTCAATACTATATTAAAACATAGC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 4
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
429 bp | 142 aa | 90 | 518 | + | No |
Description : First part of the transposase
ORF sequence :
MPNVKSILNHIEKLPLQQKEQILSTLEEILVLGSQVSQVEQEVKEFRFSKGKVCPICGSETISRNSKYNGKQGYICKSCKKSFTDFTNSATYKSKKTLDK
WLKYAKCMVNGYSIRKSAKVVEINIATSFFWRHKILDCVSTF
WLKYAKCMVNGYSIRKSAKVVEINIATSFFWRHKILDCVSTF
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
615 bp | 204 aa | 500 | 1114 | + | No |
Description : Second part of the transposase
ORF sequence :
MCKHILGKGYVDGVIEADEVFFTESFKGTKPSNMPRRSRKIGKQVKKSGISKEQVCITTAIDRQGNLIMELACKGRITSKELEKLYDGHISNESILCTDS
RKSYIQFANDLSLEHKRIKRGKHKEGLYHIQHINVLHSNLRKWMNRFNGAATKYISNYIKWFKWLQIFDTHKEIIKAKNFIVQSNVAHAYIKVKDLKYRE
PICV
RKSYIQFANDLSLEHKRIKRGKHKEGLYHIQHINVLHSNLRKWMNRFNGAATKYISNYIKWFKWLQIFDTHKEIIKAKNFIVQSNVAHAYIKVKDLKYRE
PICV
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1025 bp | 341 aa | 90 | 1114 | + | Yes |
Chemistry : DDE
ORF sequence :
MPNVKSILNHIEKLPLQQKEQILSTLEEILVLGSQVSQVEQEVKEFRFSKGKVCPICGSETISRNSKYNGKQGYICKSCKKSFTDFTNSATYKSKKTLDK
WLKYAKCMVNGYSIRKSAKVVEINIATSFFWRHKILDCVSTFLGKGYVDGVIEADEVFFTESFKGTKPSNMPRRSRKIGKQVKKSGISKEQVCITTAIDR
QGNLIMELACKGRITSKELEKLYDGHISNESILCTDSRKSYIQFANDLSLEHKRIKRGKHKEGLYHIQHINVLHSNLRKWMNRFNGAATKYISNYIKWFK
WLQIFDTHKEIIKAKNFIVQSNVAHAYIKVKDLKYREPICV
WLKYAKCMVNGYSIRKSAKVVEINIATSFFWRHKILDCVSTFLGKGYVDGVIEADEVFFTESFKGTKPSNMPRRSRKIGKQVKKSGISKEQVCITTAIDR
QGNLIMELACKGRITSKELEKLYDGHISNESILCTDSRKSYIQFANDLSLEHKRIKRGKHKEGLYHIQHINVLHSNLRKWMNRFNGAATKYISNYIKWFK
WLQIFDTHKEIIKAKNFIVQSNVAHAYIKVKDLKYREPICV
Blast result :ORF 4
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
531 bp | 176 aa | 1202 | 1732 | + | No |
Annotation : Dihydrofolate reductaseDescription :
ORF sequence :
MYNNRKLVLYIATSLDGYIATEDDSLEWLFKTEGEGDNGYFEFYNTVDTILIGRRTYDWIIEKEKGKFPYKNKKCYVFSKSEKGKNENVEFINQDVVEFT
NKIKRLDGGNIWLVGGGVLLNSFIKERLVDEFIITISPTLIGHGIPLFKKNDFELELKLKGIRRFNQFVELHYEVK
NKIKRLDGGNIWLVGGGVLLNSFIKERLVDEFIITISPTLIGHGIPLFKKNDFELELKLKGIRRFNQFVELHYEVK
Blast result :
Comments
ISCac2 is 60% aa similar to ISSag10.
There is a premature stop codon in the transposase, so the third ORF is a putative ORFAB reconstructed in silico by deletion of the STOP codon.
The ORF 4 is a passenger gene annotated as "Enzyme of dihydrofolate reductase family".
There is a premature stop codon in the transposase, so the third ORF is a putative ORFAB reconstructed in silico by deletion of the STOP codon.
The ORF 4 is a passenger gene annotated as "Enzyme of dihydrofolate reductase family".
References
1] Nolling,J., Breton,G., Omelchenko,M.V., Markarova,K.S., Zeng,Q., Gibson,R., Lee,H.M., Dubois,J., Qiu,D., Hitti,J., Wolf,Y.I., Tatusov,R.L., Sabathe,F., Doucette-Stamm,L., Soucaille,P., Daly,M.J., Bennett,G.N., Koonin,E.V. and Smith,D.R. (2001) J. Bacteriol. 183 (16), 4823-4838