ISCac2

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003030 ND Clostridium acetobutylicum
Clostridium acetobutylicum ATCC 824
DNA section
IS Length : 1838 bp

Ends


IR Length : 21/25

IRL : GGCTATGTTTTAAATCAGTGTTGAAAATAGATATATATTAGAAAAATAGG
IRR : GCTATGTTTTAATATAGTATTGAAATATAGCCTAAAAAAATTATAGGTAG

Insertion site


Left flankDirect repeatRight flankDR Length
GGCAAGTTTGCTCTTTTTTATTTTAAATAAACCGTTATATA9

DNA sequence

GGCTATGTTTTAAATCAGTGTTGAAAATAGATATATATTAGAAAAATAGGTTATAATATTAGATAGAATAGTGTTTAGGAGGTAGAATTATGCCAAATGT
GAAGTCTATATTAAATCATATTGAAAAGTTACCATTACAGCAGAAAGAACAAATACTATCAACTTTAGAAGAAATTCTTGTCCTAGGTTCACAAGTAAGC
CAAGTAGAACAAGAAGTTAAAGAATTTAGATTTTCCAAGGGGAAAGTATGCCCAATTTGTGGTTCTGAAACTATATCAAGAAATAGCAAGTATAATGGTA
AACAAGGATATATCTGCAAGTCTTGTAAAAAGTCCTTTACTGACTTCACTAATTCTGCAACCTATAAAAGTAAGAAAACATTAGATAAGTGGCTTAAATA
TGCTAAGTGCATGGTTAATGGATATTCTATCAGGAAGAGTGCTAAAGTTGTAGAAATTAATATAGCAACAAGTTTCTTTTGGAGACATAAAATTCTTGAT
TGTGTAAGCACATTTTAGGCAAGGGGTATGTTGATGGTGTTATTGAAGCAGATGAAGTATTCTTTACTGAAAGTTTTAAAGGTACAAAACCTTCTAATAT
GCCAAGACGCTCTCGTAAAATCGGCAAACAAGTTAAGAAAAGTGGTATATCAAAGGAACAAGTCTGTATTACTACTGCTATTGATAGACAAGGTAATTTA
ATTATGGAATTAGCATGTAAAGGTAGAATTACCTCTAAGGAATTAGAAAAATTATATGATGGACATATAAGCAATGAATCTATACTTTGTACTGATAGTC
GTAAAAGTTATATACAATTTGCAAATGATTTATCATTAGAACACAAACGTATAAAAAGAGGTAAACATAAGGAAGGACTATATCATATTCAACATATTAA
TGTTCTACACAGTAATTTAAGGAAATGGATGAATAGGTTTAATGGTGCTGCAACAAAATATATTAGTAACTATATTAAGTGGTTCAAATGGCTTCAAATA
TTTGATACACATAAAGAAATAATAAAGGCTAAAAACTTTATAGTTCAAAGCAATGTAGCACATGCATATATCAAAGTAAAAGACTTAAAATATAGAGAGC
CAATATGTGTATAACATATGTAATATTTTGTAAATGATGCAATAATAAGTGCTATTAACAACTATAATTAGATTGAATTTTATAAATATGGAGGTTTATA
GATGTACAACAATAGAAAATTAGTTCTATATATTGCAACAAGTTTAGATGGTTACATAGCGACAGAAGATGATTCCTTGGAATGGTTGTTTAAAACTGAA
GGCGAAGGAGATAATGGATATTTTGAATTCTACAATACAGTTGATACTATTCTAATTGGTAGAAGAACTTATGATTGGATTATTGAAAAAGAAAAAGGTA
AATTTCCTTATAAGAATAAAAAGTGTTATGTATTTTCTAAATCAGAAAAAGGTAAAAATGAAAATGTAGAATTTATTAATCAAGATGTAGTGGAATTTAC
AAATAAAATCAAAAGACTGGATGGAGGAAACATATGGCTTGTTGGCGGTGGTGTTCTTCTAAATTCTTTTATTAAAGAAAGGTTAGTTGACGAATTTATT
ATTACTATATCACCTACACTAATAGGTCATGGAATACCTTTATTTAAAAAAAATGATTTTGAGTTAGAACTAAAGTTAAAGGGGATAAGGAGATTTAATC
AATTTGTTGAACTCCATTACGAGGTGAAATAAAACAATTGATATTTAGTTCATAAATTTCTTATAGTGAGTATTATAGGTGGACAATCCTACCTATAATT
TTTTTAGGCTATATTTCAATACTATATTAAAACATAGC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 4

 

ORF 1
LengthBeginEndStrandFusion ORF
429 bp142 aa90518+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPNVKSILNHIEKLPLQQKEQILSTLEEILVLGSQVSQVEQEVKEFRFSKGKVCPICGSETISRNSKYNGKQGYICKSCKKSFTDFTNSATYKSKKTLDK
WLKYAKCMVNGYSIRKSAKVVEINIATSFFWRHKILDCVSTF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
615 bp204 aa5001114+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MCKHILGKGYVDGVIEADEVFFTESFKGTKPSNMPRRSRKIGKQVKKSGISKEQVCITTAIDRQGNLIMELACKGRITSKELEKLYDGHISNESILCTDS
RKSYIQFANDLSLEHKRIKRGKHKEGLYHIQHINVLHSNLRKWMNRFNGAATKYISNYIKWFKWLQIFDTHKEIIKAKNFIVQSNVAHAYIKVKDLKYRE
PICV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1025 bp341 aa901114+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPNVKSILNHIEKLPLQQKEQILSTLEEILVLGSQVSQVEQEVKEFRFSKGKVCPICGSETISRNSKYNGKQGYICKSCKKSFTDFTNSATYKSKKTLDK
WLKYAKCMVNGYSIRKSAKVVEINIATSFFWRHKILDCVSTFLGKGYVDGVIEADEVFFTESFKGTKPSNMPRRSRKIGKQVKKSGISKEQVCITTAIDR
QGNLIMELACKGRITSKELEKLYDGHISNESILCTDSRKSYIQFANDLSLEHKRIKRGKHKEGLYHIQHINVLHSNLRKWMNRFNGAATKYISNYIKWFK
WLQIFDTHKEIIKAKNFIVQSNVAHAYIKVKDLKYREPICV

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
531 bp176 aa12021732+No
ORF function : Passenger Gene
Annotation : Dihydrofolate reductaseDescription :

ORF sequence :

MYNNRKLVLYIATSLDGYIATEDDSLEWLFKTEGEGDNGYFEFYNTVDTILIGRRTYDWIIEKEKGKFPYKNKKCYVFSKSEKGKNENVEFINQDVVEFT
NKIKRLDGGNIWLVGGGVLLNSFIKERLVDEFIITISPTLIGHGIPLFKKNDFELELKLKGIRRFNQFVELHYEVK

 

Blast result :
Comments
ISCac2 is 60% aa similar to ISSag10.
There is a premature stop codon in the transposase, so the third ORF is a putative ORFAB reconstructed in silico by deletion of the STOP codon.
The ORF 4 is a passenger gene annotated as "Enzyme of dihydrofolate reductase family".
References
1] Nolling,J., Breton,G., Omelchenko,M.V., Markarova,K.S., Zeng,Q., Gibson,R., Lee,H.M., Dubois,J., Qiu,D., Hitti,J., Wolf,Y.I., Tatusov,R.L., Sabathe,F., Doucette-Stamm,L., Soucaille,P., Daly,M.J., Bennett,G.N., Koonin,E.V. and Smith,D.R. (2001) J. Bacteriol. 183 (16), 4823-4838