ISfinder Mirror Site

ISBthe6

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_004663	ND	Bacteroides thetaiotaomicron	Bacteroides thetaiotaomicron VPI-5482

DNA section

IS Length : 2544 bp

Ends

IR Length : 24

IRL : GTAAGCCCCCGATAAAGTACCCGTTGTCTTTGTATCTTTAAGTCTCGATC
IRR : GTAAGCCCCCGATAAAGTACCCGTATAAAAAAGAATCGCTATAGCAAATC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTCAATACAG	ATAACAAT	ATTGCCATTC	8
TGACAAGGTT	ATTGCTTG	TAGTATTTTC	8

DNA sequence

GTAAGCCCCCGATAAAGTACCCGTTGTCTTTGTATCTTTAAGTCTCGATCTTTGCCAGAAAAAAGACTATGCAAAAAATGAGTAAAGAAGAATTCATTGA
AATCCTGTCTCGTCAACAGCGTAGCGGTTTGACGATAAAAGACTTCTGTATAAATGAAGCCTATACCGAATCAAGCTTTTACTATTGGAAAGGAAAGTTT
GGCCTATCGAGGCGTTATCATATGGATAGGCATTCTTCCTCTTTAGAGGAGTTTGCACCGGTTAGCCTCACCTCTTCACCTGCTTCCCACTCTGCCTGTG
ATAGTGGCGCTATACAAACGGGTGAGATCCGGATAGAGTTCCCCGGTGGTATTATCGCCCACTTCAGTGGTATGGCTGAATCCCAGGCGGCCATGCAATT
GCTCACTCAACTCTGCAATCGCCATGTTTTGCCTGAATGACACGATGCGCTACTTTCTCTGTCCGGGTAAGACAGATATGCGTAAAGGTATGAACTCATT
ATGCGGGGTTGTTCATGATAAAATGGGATATGATGTCCGTTTAGGTGATGTGTTTATCTTTATCAACCGCCAGCGCACTACAATGAAACTGCTACACGCG
GAAGATGGCGGACTGGTTTTGTACATAAAACGACTTGAAGAAGGCACTTTCCGCCTTCCCTCTTATGACAAAGAAAGCAAGTCGTACCCTATGCAGTGGC
GTGACCTGGTTCTGATGGTAGAGGGAATCAACGACGAACCTTCCAAAAGGCTCAAACGTCTGAAAGCGTTACGAAAAAGTGACATGCAGTACTGAAAAAC
AGCAGGTGAAACCTTGATTATACGGTTGCTTTTCTGTATTTTTGTATTGTTCAAAACAGGTATGACGAATGACTCAGACAGAAACATTAGAACTTTTGGT
GGCCACACTCCAGCAGGCTAATAGCTCCCAGTCTGAGTCAATCGAGCGTCTGACCCGGCAGAACGAACAGCTGCAAAATAAGCTTCAGGAACTGCTGGCT
CAGGTAGCCTGGTTAAATCGTCAGTTATTCGGCCGTAAGAGCGAGAAATTAGCCCATCTGGATCCGAACCAGCTATCACTGTTCGATCCACCTGTTCAGC
CGTTGGAACACGAAATACCGGAAGAAGCCGCAGCCCAAGAACCTGTCTGCTCGACGACTCCCAAAAAGAAAGTGCGTCAGAACCGCAACATGTTGGATGG
CCTTCCTGTAGTGGAGATTGTCATAGAACCCGAAGGAGTCGATCCGGATAAATACAAGCGCATAGGCGAGGAACGCACACGTACACTTGAATTTGAACCG
GGCAAATTATACGTAAAAGAGATCATACGTCCCAAGTATGGCCTGAAAGACAATATAAGTTTGCCTCAGGGGCATCAGGGCAGTGTTATTATAGCCCCTC
TTCCGCTATTGCCTATCTACAAGGGACTTCCCGGTGCCAGCCTGCTCACTGAAATCCTCTTGCAAAAATATGAATATCATGTACCATTCTATCGTCAGGT
GCGTGAGTTTCACCATTTAGGCCTGAAGATCTCGGAAAACACGCTTCAGGGGTGGTTCAAACCTGCCTGTGAATTACTTAAACCTCTCTATGAAGAGTTG
AAGAAACAGGTATTGAAGGCCGACTATATCCAGGTGGACGAAACGACATTACCGGTTATCAACAAACAGAATCATAAAGCGGTTAAGGAATACTTGTGGA
TAGTCAGGGCGGTTATGGATGGATTGGTCTTCTTTCATTATGATGACGGTTCCCGCTCACAGGAAACAGCCTGGAAATTATTACAAACCTTCAAAGGATA
TCTTCAAAGTGACGGCTATGCGGCCTACAACATCTTTGAGGGTAAGAAAGAGGTGTGCCTTGTCGGATGCCTTGCCCACATAAGGCGACATTACGAGGTT
GCCAAAGAAGAGAATGAATCCCTGGCCGGATATGTTCTGGCTCAAATACAGCAACTCTATCGGATCGAACAGATTGCCGACCAGGAGGAACTCACTTATG
AGCAACGCATGCTTAGAAGACAGGAACAGGCACTTCCCATACTTGAGCAACTGGAAAAATGGATGGAAACAGCCTATCCGAAAGTGCTTCCTAAAAGCCG
GATGGGGCAAGCTATCGCTTACGCGTATCAACTTTGGCCACGTATGAGGAATTATCTGAAAGACGGCAGGCTTAAAATAGATAATAATCTGGCCGAAAAT
GCGATTCGTCCGATAGCCTTATCAAGAAAAAACTTCTTATTTTGTGGAAACCATGAAGCCGCGCAGAACACTGCCATAATCTGTTCGCTCTTGGCATCAT
GCAAAGCCTCCAACATTAACCCCCGGGAATGGCTCACGGAGGTGATTGCACTATTGCCGTATTATGCAGCCAACAAGGAGAAAGACCTAAAAGAGCTGCT
ACCCCATTGCTGGGAATCGGGAAACTCCAAAGAACTCTAATAATACTCTAACGAAAAACAAGAATATAAATACAGAATCACTATAGCTTTGTTCGATTTG
CTATAGCGATTCTTTTTTATACGGGTACTTTATCGGGGGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
372 bp	123 aa	69	440	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MQKMSKEEFIEILSRQQRSGLTIKDFCINEAYTESSFYYWKGKFGLSRRYHMDRHSSSLEEFAPVSLTSSPASHSACDSGAIQTGEIRIEFPGGIIAHFS
GMAESQAAMQLLTQLCNRHVLPE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
351 bp	116 aa	445	795	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MRYFLCPGKTDMRKGMNSLCGVVHDKMGYDVRLGDVFIFINRQRTTMKLLHAEDGGLVLYIKRLEEGTFRLPSYDKESKSYPMQWRDLVLMVEGINDEPS
KRLKRLKALRKSDMQY

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1572 bp	523 aa	869	2440	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTQTETLELLVATLQQANSSQSESIERLTRQNEQLQNKLQELLAQVAWLNRQLFGRKSEKLAHLDPNQLSLFDPPVQPLEHEIPEEAAAQEPVCSTTPKK
KVRQNRNMLDGLPVVEIVIEPEGVDPDKYKRIGEERTRTLEFEPGKLYVKEIIRPKYGLKDNISLPQGHQGSVIIAPLPLLPIYKGLPGASLLTEILLQK
YEYHVPFYRQVREFHHLGLKISENTLQGWFKPACELLKPLYEELKKQVLKADYIQVDETTLPVINKQNHKAVKEYLWIVRAVMDGLVFFHYDDGSRSQET
AWKLLQTFKGYLQSDGYAAYNIFEGKKEVCLVGCLAHIRRHYEVAKEENESLAGYVLAQIQQLYRIEQIADQEELTYEQRMLRRQEQALPILEQLEKWME
TAYPKVLPKSRMGQAIAYAYQLWPRMRNYLKDGRLKIDNNLAENAIRPIALSRKNFLFCGNHEAAQNTAIICSLLASCKASNINPREWLTEVIALLPYYA
ANKEKDLKELLPHCWESGNSKEL

Blast result :

Comments

ISBthe6 (orf1) is 84% aa similar to ISBthe5 (orf1).
ISBthe6 (orf2) is 96% aa similar to ISBthe5 (orf2).
ISBthe6 (orf3) is 87% aa similar to ISBthe5 (orf3).

References

1] Xu,J., Bjursell,M.K., Himrod,J., Deng,S., Carmichael,L.K., Chiang,H.C., Hooper,L.V. and Gordon,J.I. (2003) Science 299 (5615), 2074-2076.

2] Xu,J., Bjursell,M.K., Himrod,J., Deng,S., Carmichael,L.K., Chiang,H.C., Hooper,L.V. and Gordon,J.I. (2002) Direct Submission GenBank.

3] ISfinder annotation (2008).