ISBsp1
- Family IS1595
- Group ISPna2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ABCF01000016 | ND | Bacillus sp. | Bacillus sp. SG-1 |
DNA section
IS Length : 1665 bp
Ends
IR Length : 25/28
IRL : GGCTCTTTTCGCATAGATTGTTGCTATTTTGACCATCCTTATAAGAGGTG
IRR : GGCTCTTTTCGTAAAGATTGTTGCTTTTAATCATACAAGGAATATTTTAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCCAAAGTCCCCCGTAT | AAAAAGAA | AGACCTTCCGCGAAAC | 8 |
DNA sequence
GGCTCTTTTCGCATAGATTGTTGCTATTTTGACCATCCTTATAAGAGGTGGTAAAATACTCTTTCAGGAGGCGGTTTCTCAATGATTAAAGACATCTATG
CCACGTTTTCAGACTTAACTAAACACGAACAAATCCAACTATTTAACCTTTTAAGAGAAGACTTTATCGATAATCAAGATGCTGATATGAAAGATGCATA
CAAAACAATTAGAGAAACCCGTTTTAAAGAGGGATTAGGCTGCATTCATTGCGGAAGTGTGAAGGTAAAGAGAAATGGCAAGTACAGAGAGAGGCAAAGA
TATCTTTGTCGTGACTGCGGAAAGTCATTTAACGAACTTTCAAATACTCCGATTGCAGGAACCCGTTATCTTGGTAAGTGGGCGAAGTATTTTCATATGA
TGGTGGAAGGCTATACGTTGCCTAAGATTGCTAAACGCTTAAAAATCCACATCTCTACTGCATTTTACTGGAGACATAAAATTCTTTTTGCTTTGCGTTC
AATGGGTTATCAAATGTTGAAAGGGATTATTGAAAGTGACGAAACCTTCTTTAAAGAGTCTTTAAAAGGACGAAGAACACTGGACAGGAAACCTAAAAAA
CGTGGTGGCAGAGATAAATTGAGAGGTATTAGTCATCTTAAAATTGCTGTTGTGGTCGCTCAAGACCGAAATGGTCAAGTCATTGCTCAAAAGGCTGGAA
ATGGTCGTGTAAAAGAAGAAGAAATTGACGCAGTTATCGGAAGCTATATTGACCCGTCTTCTTTGTTATGCACAGATACAGCCACGAACTACAAGAAATT
CGCTCAAATCAAGGCACTTAAACACGAACCGATAAACCTTAGCAAAGAGGGGTATGTCAAAAAGGGCATCTACCATTTGCAAAACGTCAATAACTATCAT
AAACGTTTAAAAGGCTGGATGGATAGGTTCCAAGGAGTGGCTACAAAGTATTTGGATAATTATCTTTACTGGTTCAGCTTCCTTCAGCAAAGTAAGAAGC
TGGCTGAAAAGGAGCAGATAAACCAAATGCTTCTTAATGCTTGCCAAAATTCAAATAGTATTACCGTTAATTTCTTACGTGAGGTATAAAAAATCAGCCA
TTCCTATATGGAACGACTGTATTTCTTATTCCGTTAAGGCCTGACTTTTACTCAAAGGAGTTTGATTTGCTTTTTATTTCACCTTCAGGGGTTACGCTGT
ATTGTGCGATAGAACTTGTTGCTTCTCCCTCATTTGTTAGCCCGTCCTTTAAATTGAACGTTACTTCCCACGCTTTTGAGGAACTACTGAGGTGTGTATC
ATTTGAAAGTAACTCAACATTATCCATCTCTATATCCTCGTTGCTCTTCTCATTGAATTGTGCTAAGTCTTTCTCGAACTCCTCCATAGCAATTTCTTCA
GCTTGTTCCTTAGTTATTTCTTCTGTCTCATTATTTCCACAGCCGACCAAAGAGAAAATTGCTAATAAGGATACTAATATAACAGATATTCTTTTCATAA
ATTTACCCCTCCTATTAATTAACTTTACGATAACAAACAGTAAAAAGTTTCATTTTTTTCAACTTCTAAAAACTGCCCGCTCGAATAAGCCTTTCTTTAT
ATAATACCATAAAAAGTAAAATATTCCTTGTATGATTAAAAGCAACAATCTTTACGAAAAGAGCC
CCACGTTTTCAGACTTAACTAAACACGAACAAATCCAACTATTTAACCTTTTAAGAGAAGACTTTATCGATAATCAAGATGCTGATATGAAAGATGCATA
CAAAACAATTAGAGAAACCCGTTTTAAAGAGGGATTAGGCTGCATTCATTGCGGAAGTGTGAAGGTAAAGAGAAATGGCAAGTACAGAGAGAGGCAAAGA
TATCTTTGTCGTGACTGCGGAAAGTCATTTAACGAACTTTCAAATACTCCGATTGCAGGAACCCGTTATCTTGGTAAGTGGGCGAAGTATTTTCATATGA
TGGTGGAAGGCTATACGTTGCCTAAGATTGCTAAACGCTTAAAAATCCACATCTCTACTGCATTTTACTGGAGACATAAAATTCTTTTTGCTTTGCGTTC
AATGGGTTATCAAATGTTGAAAGGGATTATTGAAAGTGACGAAACCTTCTTTAAAGAGTCTTTAAAAGGACGAAGAACACTGGACAGGAAACCTAAAAAA
CGTGGTGGCAGAGATAAATTGAGAGGTATTAGTCATCTTAAAATTGCTGTTGTGGTCGCTCAAGACCGAAATGGTCAAGTCATTGCTCAAAAGGCTGGAA
ATGGTCGTGTAAAAGAAGAAGAAATTGACGCAGTTATCGGAAGCTATATTGACCCGTCTTCTTTGTTATGCACAGATACAGCCACGAACTACAAGAAATT
CGCTCAAATCAAGGCACTTAAACACGAACCGATAAACCTTAGCAAAGAGGGGTATGTCAAAAAGGGCATCTACCATTTGCAAAACGTCAATAACTATCAT
AAACGTTTAAAAGGCTGGATGGATAGGTTCCAAGGAGTGGCTACAAAGTATTTGGATAATTATCTTTACTGGTTCAGCTTCCTTCAGCAAAGTAAGAAGC
TGGCTGAAAAGGAGCAGATAAACCAAATGCTTCTTAATGCTTGCCAAAATTCAAATAGTATTACCGTTAATTTCTTACGTGAGGTATAAAAAATCAGCCA
TTCCTATATGGAACGACTGTATTTCTTATTCCGTTAAGGCCTGACTTTTACTCAAAGGAGTTTGATTTGCTTTTTATTTCACCTTCAGGGGTTACGCTGT
ATTGTGCGATAGAACTTGTTGCTTCTCCCTCATTTGTTAGCCCGTCCTTTAAATTGAACGTTACTTCCCACGCTTTTGAGGAACTACTGAGGTGTGTATC
ATTTGAAAGTAACTCAACATTATCCATCTCTATATCCTCGTTGCTCTTCTCATTGAATTGTGCTAAGTCTTTCTCGAACTCCTCCATAGCAATTTCTTCA
GCTTGTTCCTTAGTTATTTCTTCTGTCTCATTATTTCCACAGCCGACCAAAGAGAAAATTGCTAATAAGGATACTAATATAACAGATATTCTTTTCATAA
ATTTACCCCTCCTATTAATTAACTTTACGATAACAAACAGTAAAAAGTTTCATTTTTTTCAACTTCTAAAAACTGCCCGCTCGAATAAGCCTTTCTTTAT
ATAATACCATAAAAAGTAAAATATTCCTTGTATGATTAAAAGCAACAATCTTTACGAAAAGAGCC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1008 bp | 335 aa | 82 | 1089 | + | No |
Chemistry : DDE
ORF sequence :
MIKDIYATFSDLTKHEQIQLFNLLREDFIDNQDADMKDAYKTIRETRFKEGLGCIHCGSVKVKRNGKYRERQRYLCRDCGKSFNELSNTPIAGTRYLGKW
AKYFHMMVEGYTLPKIAKRLKIHISTAFYWRHKILFALRSMGYQMLKGIIESDETFFKESLKGRRTLDRKPKKRGGRDKLRGISHLKIAVVVAQDRNGQV
IAQKAGNGRVKEEEIDAVIGSYIDPSSLLCTDTATNYKKFAQIKALKHEPINLSKEGYVKKGIYHLQNVNNYHKRLKGWMDRFQGVATKYLDNYLYWFSF
LQQSKKLAEKEQINQMLLNACQNSNSITVNFLREV
AKYFHMMVEGYTLPKIAKRLKIHISTAFYWRHKILFALRSMGYQMLKGIIESDETFFKESLKGRRTLDRKPKKRGGRDKLRGISHLKIAVVVAQDRNGQV
IAQKAGNGRVKEEEIDAVIGSYIDPSSLLCTDTATNYKKFAQIKALKHEPINLSKEGYVKKGIYHLQNVNNYHKRLKGWMDRFQGVATKYLDNYLYWFSF
LQQSKKLAEKEQINQMLLNACQNSNSITVNFLREV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 351 bp | 116 aa | 1498 | 1148 | - | No |
Annotation : Hypothetical proteinDescription :
ORF sequence :
MKRISVILVSLLAIFSLVGCGNNETEEITKEQAEEIAMEEFEKDLAQFNEKSNEDIEMDNVELLSNDTHLSSSSKAWEVTFNLKDGLTNEGEATSSIAQY
SVTPEGEIKSKSNSFE
SVTPEGEIKSKSNSFE
Blast result :
Comments
ISBsp1 is 61% aa similar to ISBwe1.
There is a second ORF on complementary strand, annotated as hypothetical protein.
There is a second ORF on complementary strand, annotated as hypothetical protein.
References
1] Tebo,B., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.