ISfinder Mirror Site

ISBf1

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
U05888	ND	Bacteroides fragilis	Bacteroides fragilis CS30 Bacteroides fragilis RBF103

DNA section

IS Length : 2787 bp

Ends

IR Length : 6/8

IRL : TGTGGGCCATCCATTATCAATAGCCACGTTTACCATCGTCCGCAGGGGAG
IRR : TGTGGCGCGCAAATTTGTGCGCCACTTTTTTAGGTAATTTAGTATATCCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

TGTGGGCCATCCATTATCAATAGCCACGTTTACCATCGTCCGCAGGGGAGCCGTATAAATCCATACCGTATTATTACAACAATGCCTGTCGGTCCGTAGA
TACATATCAAAGTCTTTGATAAACGCCAATGTCAGTTCCTTCAGCGCAATATCTTTCAAATGTAACCGTTGGTAAAGAAAATCTTTCAGATGATTATACA
CGGTCACATACTTCTCATACGTCTTGACAGATTTCATCTTTTTCATCGCATTGTAGATTGTAACTTTACCAGTGGAAAAACGGAAAGTCGTATGTCTCAC
TTTTACAGGGGCATGATTTTTCTGCCTTGCGGGAATGTCTATCTTTGCCGGCTCAACAAAGAGACAGACACCGGAATGGAAACAAAGAGCCAAAATTTAA
AAGACAAACTGTATATGTGGTACAAGGTAAGAGAACTTCAGTCGAAAGGACTGAACAAGACACAGATCGGAAAATATCTGGGCGTGGACCGGAGCACTGT
GCGGAGATATCTGCGGACAAGCCGGGAAGAGCTTTTCAGAAAGCAGAACTCCCACCGGGAGTATGAACTCAAGTTGGGAAAGTACGAGGAGTATGTACGG
GGTACACTGGAAGAATACCCGTACATATCGGCCGCCCGTATGCATGACTGGCTCAAGGAATGCTATCCGGATTTTCCTCGGGTATGTGACAAGACCGTAT
TCAATTTTGTGGACAGGATACGCAGAAAGTACGGTATCGGGAAAAAATCTGAGGTCCGGATACGGCGTGATTACGAAAAGTTGCCTGAGACCCCCTACGG
GGAATATGCCCAGGCGGATTTCGGGGAGAAGTGGATACCTGTGAAGAATGGCGGAAGCACGAAGGTTTACTTCTTCGCCATCGTACTGACGCGTTCCCGC
TATAAGTTTATCCATTTCAGCCGTCGTCCTTTTGATACGGAGCTTGCGATTTATGCCCATGAACTGGCCTTTCAGTATTTCGGGGGCAGGCCTGAAAAGA
TTATCTATGACCAGGACAGGGTGCTTATAGCACGCGAGAACCTGGGAGATTTGATACTGACCGGGAAATTTCAGTCATTCATAAAAGAGCAGCATTTCCA
GCCGGTATTTTGTCGCAGGTCAGACCCTGAGTCGAAGGGGAAAGTGGAGAATGTGGTGAAGTATGTGAAAGAGAATTTTCTGGTGGCCCGTGTTTTACAG
GATATCCCCGGGCTGAATGAGGAAGCCCGGAAATGGCTTGAAAGGACGGGTAATGGAAAAGTGCACGGGACCACCCGCCTGGTCCCCTCTGAGGAGTTCG
CTGTGGAGAAAGGATACCTGAAACCTTATTATGGTCTACCGCAGCCACCACAGGAGCAGATGAAGGAGTATCACGTGCGTAAGGACAACACGGTCCAGTA
TAGGGGGAATTATTACAGCCTGCCATGCGGCACTTACCGGAGCGGGCAGACAAGGGTCTGGTTGCAGGAAACTGAGGGGTATGTGGAACTGTACAGCAAG
GAGACGGGAAAAATCGTCGCCAGACATCCCCTCTGTACCCGAAAAGGAAAAACGATTTATGACGAAAGACACAGAAGGCCCAAGAGTATTGGGGCACAGA
AGCTGGCCGAACGTATCCTTGTTTATGTATCCGGAAACCGGGAGGTCGCCTTGTGGATGGAGAACCTCAAGAGAAAGAAAGAACGCTATTACAAGGATAA
TCTGGAAGTGGTTCTGCACATGATGCCGGGCTATGACAAGGATATCTTGATAGAGGCAGTGCACATATGTCTTGATAAGGGCATCTACAATGGCGATTCC
GTTAAAAGCCTGTGTGAACACGTGCACAGGAGACGGAATAAAGAAACTGAAACAGACAGGACGGACAGTTGCCCGCCGCGACAAACCGGGCTGATACAGT
CTTACAATGAAATCTTCCATGGCTATGATAAAACATAAAAAGGAGCTGGTTGAATATGCACGGCACCTGAAACTACCGAACTTGGCAGAACATGTGGGTG
TGATACTTCATGAAGCACAGGAGAAGCAACTGACCTATTCGGAGTTCCTGGCGAATTGTCTTGCCAGGGAAATCCAGGGCAGAGAAAGGAAAAGCTACCT
GACACGGTTGAAACTCTCCGGACTCCCCGCCAAATACGATCTGGACCTGTACGACTATGACAGGGCGGAAGGAATGGATAACAGGAGGCTGCGCGAACTG
CGTGAGCTGGTATGGGTGAACCAGGCATACAATCTTCTCCTGGTAGGCCCTTCCGGAACCGGCAAGACCTTTATAGCCGCCGGACTCGTGTATGAAGCCG
TAAAAGCGGGATACGAAGCATATCTGATGACACTGGAGGAGCTGCTCACATGCCTGAAAACGAAAGAGGTCTCCGCACATGCGATGAAAACATACAAGCG
GATCATGAAGGCCCGGCTGCTGGCCATAGACGATGCCACACTGTTCCCTTTGAAAAGAGAAGAAGCCGTGTTGCTGTTCAAACTGGTCAATGACTTCCAG
GAAAGGACATCACTCATCATCACGGCAAACAAGGCACTCACCCGTTGGCTGGAAACATTGGAGGATGAAGCGGTCACAGCCGCCTTGCTTGACAGGCTGC
TCTACTGCTGCGAGATTATCAGGCTCGGAGGAACAAGCTATCGCATGCAAAACAGGAAAACAATTTTTAGCAACCAAAACACGGATATAGGCACGTAAAA
AGAGTTAAGGAAAGTGAAGCATCTTCGATGCTGGAGTGGGATATACTAAATTACCTAAAAAAGTGGCGCACAAATTTGCGCGCCACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1749 bp	582 aa	190	1938	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIIHGHILLIRLDRFHLFHRIVDCNFTSGKTESRMSHFYRGMIFLPCGNVYLCRLNKETDTGMETKSQNLKDKLYMWYKVRELQSKGLNKTQIGKYLGVD
RSTVRRYLRTSREELFRKQNSHREYELKLGKYEEYVRGTLEEYPYISAARMHDWLKECYPDFPRVCDKTVFNFVDRIRRKYGIGKKSEVRIRRDYEKLPE
TPYGEYAQADFGEKWIPVKNGGSTKVYFFAIVLTRSRYKFIHFSRRPFDTELAIYAHELAFQYFGGRPEKIIYDQDRVLIARENLGDLILTGKFQSFIKE
QHFQPVFCRRSDPESKGKVENVVKYVKENFLVARVLQDIPGLNEEARKWLERTGNGKVHGTTRLVPSEEFAVEKGYLKPYYGLPQPPQEQMKEYHVRKDN
TVQYRGNYYSLPCGTYRSGQTRVWLQETEGYVELYSKETGKIVARHPLCTRKGKTIYDERHRRPKSIGAQKLAERILVYVSGNREVALWMENLKRKKERY
YKDNLEVVLHMMPGYDKDILIEAVHICLDKGIYNGDSVKSLCEHVHRRRNKETETDRTDSCPPRQTGLIQSYNEIFHGYDKT

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
792 bp	263 aa	1907	2698	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MKSSMAMIKHKKELVEYARHLKLPNLAEHVGVILHEAQEKQLTYSEFLANCLAREIQGRERKSYLTRLKLSGLPAKYDLDLYDYDRAEGMDNRRLRELRE
LVWVNQAYNLLLVGPSGTGKTFIAAGLVYEAVKAGYEAYLMTLEELLTCLKTKEVSAHAMKTYKRIMKARLLAIDDATLFPLKREEAVLLFKLVNDFQER
TSLIITANKALTRWLETLEDEAVTAALLDRLLYCCEIIRLGGTSYRMQNRKTIFSNQNTDIGT

Blast result :

Comments

ISBf1 is located 50 bp upstream the translation start codon of cepA, a chromosomal cephalosporinase gene, whose high expression level is apparently driven by an ISBf1 outward promoter. The exact limits of ISBf1 are unknown.

References

1] Rogers, M.B., Bennette, T.K., Payne, C.M., and Smith, C.J. (1994) J. Bacteriol. 176, 4376-4384.