ISfinder Mirror Site

ISAzo26

Family IS91
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 Azoarcus sp. EbN1 plasmid 2

DNA section

IS Length : 2604 bp

Ends

oriIS : TGGACTAAACCGCTGGCGCGGTAGGGGGTGATCCACGCGCGGTGCCGAAGCCGGCACATTCCCACAAAGCCCGCGTTGGAGAAGGATGGGGTGGCTGTTC II struct. : No
terIS : GCCTACCGAACGCGGTCATCCCGGAGCGCCCGCATCAGCACCTGCTCGCCGCGTTCGCCAGGCTAAAAACCCTCTGCATTATCCTCGGTCAACTATTAGG II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TCGATAACAA GAAC		CAAG GTCTGCCGCC	0
TCCATAAGGGTCGT		AAAG TCGCTGACGT	0

DNA sequence

TGGACTAAACCGCTGGCGCGGTAGGGGGTGATCCACGCGCGGTGCCGAAGCCGGCACATTCCCACAAAGCCCGCGTTGGAGAAGGATGGGGTGGCTGTTC
GCAGCCCTTCGTTCCTTCCCAATCAGGAGCTTTGCCATGACACCCCTTCGCCAGCGCATGCTCGAAGACATGGGCGTTCGCAATCTCGCCCCGAACACCC
AGTCGGCCTACCTGAAGCAAGTCTCCGCCTATGCAAGGTACTTCGATTGCTCGCCCGACGAACTCGGGCCGGAGCAGGTTCGCGCCTATCAGGTCTATCT
GACGCAGACCCGCAAACTCACCCCCTCCACGGTCAGTGTCGCGACCGGCGCGCTGCGATTTCTCTACAAGGTGACACTCAAGCGCGCCTGGGCGGTGGAA
GAGATTCCAATGCCCAAGCGGTCCTTCAAGCTCCCGGTGGTACTGAGCCGCGAAGAGGTGTCGCAGTTCCTGGCGTCGGTCGACCATCTCATGCATCGCA
CGATCCTGATGACGGCCTATGCCGCCGGGCTGCGCGTCTCGGAAGCGACCCACCTCAAGGTGAGCGACATCGACAGCCAGCGCATGGTCTTGCGGGTCGA
CCAGGGCAAAGGACGCAAGGATCGCTACGTGATGCTCTCGCCGCGGCTGCTCGAGGCGTTGCGGGTCTACTGGAAGTCCGCGCGGCCAACGCTGTGGCTG
TTTCCGGGTGACGTGCCGGGTCAGCCGATCACCCGCAATGCGGTGGGATTGGCGTGCCAGAAGGCTCGGCGCGCCTGCGGCATTCGGAAACCGATTACCC
CGCACTCGCTTCGACATGCCTTCGCCACACACCTGCTCGAATCCGGCACCGACGTGCGCACCATCCAGTTGCTGCTCGGCCATCGCAGTCTCGCCACGAC
CTCGCGCTACCTGAAGGTCGCCACCAGCACCGTGTGCGCGACGACGAGCCCGTTCGACCTGTTGCCGAAAGTGGTGGTGGATCCGCCGCAGCCGAGTCCG
CCCGTCCATTTCTGAGACCGGGGCGTGCGCGCCACCGGGCTCGAGGTGGCGGACATCTTTCGTCAGGCCGGGCCCATCTATCGCCGGGACCACGCGGCTA
CCCTCAGCCGCGGGCAGTACCAGGTCATGCGCGCGATCGAGCGTTGCCGCACCGCGGCCCTCGGCGGACACGTCGAGCAGTGCGACGCCTGCGGTCACCA
GCGCATCGCGTTCAATAGCTGCCGCAATCGGCACTGTCCCAAATGTCAGTCCCTGGTGCGGGCGCAATGGCTCGAAGCGCGCCAGGCCGATCTGCTCCCC
GTCGAGTATTTCCACGTCGTCTTCACCGTCCCCGAGGACATCGCGGCGATCAGCTATCAGAACAAGAGCGTGGTCTACGAGATCCTGTTTCACGCCACCG
CCGAGACCTTGCGCATCATCGCCGCCGATCCGAGGCACCTCGGGGCCGAGCTCGGTTTCATCGCCATCCTGCATACCTGGGGGCAGAATATGCTGCATCA
CCCGCATCTGCACTGCGTCGTGCCGGGCGGGGGCGTGTCGGCCGACGGCGAGCGCTGGATCGCGTGCCGCCGCGGGTTCTTCCTGCCCGTGCGGGTGCTG
TCGCGGCTGTTCCGGCACCTGTTCCTCAAGCGCTTGCAACAGGCCTTTGATGCGGGCGAGCTCCACTTCTTCAATGCCCTCGCGGCGCTGCACGCGCCCG
CTGTCTTCGCCCGGCATCTGGCGCGCGCGAGACGATCCGAGTGGGTGGTTTTCGCCAAACCGCCGTTCGGCGGCCCGCAGCAGGTCCTCGAATATCTTGG
CCGCTATACCCATCGCGTCGCCATCTCGAACAATCGCCTCGTCGCCTGCACCGACGATGAGGTGAGCTTTCGCTGGAAGGACTACCGCCACCACGGGCGC
AACAAAGTCATGCGCCTGGAGGCGCACGAATTCATCCGCCGCTTCCTGCTGCATGTCCTGCCGGCGGGGTTCCATCGTATCCGGCATTACGGATTTCTCG
CCAACCGCCTTCGCGCGAGCGCGCTCGCCCACTGCCGGGCGTTGCTCAGCGCCCCCGCGCCAGAACCGCAGACCGACGCGCCGCGCGACTACCGTGAGCG
CTACCAGCAGCTCACCGGAAGGTCACTACGCGACTGCCCGCAATGTGGCCGGGGTCACATGGTGTGCCTCGAGACCTTCCTGCCCGGCGCCTTGCCCCGC
GCGCCGCCGCGAGTGCCGTCATGAATGCGACCGCTGCCGATTTCCTGAACGCTTCCCTGGCATCCAAGAGAGGCGCCGGGACTCCTGTCGCCAGGCACGG
CCGAAACCGCCGGAGCGGCTCCGCGTCGTCGGCGCGGTGCTCGCCCGGGCAGCCACCCCAGGCATCCGAAGGCGCCGCAATCATCGCTCGGGCGTCATTT
CCACGAGCCATTCCGGCAGCCATTCACGCCTTCGCCGCTCGCACCACGATTCAATCCCCATAGCCCCACCCGCGTGCCGGCGGTTCAGTCCAACAGGCTT
TTTAGCCTACCGAACGCGGTCATCCCGGAGCGCCCGCATCAGCACCTGCTCGCCGCGTTCGCCAGGCTAAAAACCCTCTGCATTATCCTCGGTCAACTAT
TAGG

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
930 bp	309 aa	86	1015	+	No

ORF function : Accessory Gene
AG : IS91 integrase/resolvase

ORF sequence :

MGWLFAALRSFPIRSFAMTPLRQRMLEDMGVRNLAPNTQSAYLKQVSAYARYFDCSPDELGPEQVRAYQVYLTQTRKLTPSTVSVATGALRFLYKVTLKR
AWAVEEIPMPKRSFKLPVVLSREEVSQFLASVDHLMHRTILMTAYAAGLRVSEATHLKVSDIDSQRMVLRVDQGKGRKDRYVMLSPRLLEALRVYWKSAR
PTLWLFPGDVPGQPITRNAVGLACQKARRACGIRKPITPHSLRHAFATHLLESGTDVRTIQLLLGHRSLATTSRYLKVATSTVCATTSPFDLLPKVVVDP
PQPSPPVHF

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1200 bp	399 aa	1025	2224	+	No

ORF function : Transposase
Chemistry : Y2

ORF sequence :

VRATGLEVADIFRQAGPIYRRDHAATLSRGQYQVMRAIERCRTAALGGHVEQCDACGHQRIAFNSCRNRHCPKCQSLVRAQWLEARQADLLPVEYFHVVF
TVPEDIAAISYQNKSVVYEILFHATAETLRIIAADPRHLGAELGFIAILHTWGQNMLHHPHLHCVVPGGGVSADGERWIACRRGFFLPVRVLSRLFRHLF
LKRLQQAFDAGELHFFNALAALHAPAVFARHLARARRSEWVVFAKPPFGGPQQVLEYLGRYTHRVAISNNRLVACTDDEVSFRWKDYRHHGRNKVMRLEA
HEFIRRFLLHVLPAGFHRIRHYGFLANRLRASALAHCRALLSAPAPEPQTDAPRDYRERYQQLTGRSLRDCPQCGRGHMVCLETFLPGALPRAPPRVPS

Blast result :

Comments

ISAzo26 is 58%(orfA) aa similar to ISSod25 and 56%(orfB, the transposase) aa similar to ISWz1.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).