ISfinder Mirror Site

ISAzo19

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 Azoarcus sp. EbN1 plasmid 2

DNA section

IS Length : 2423 bp

Ends

IR Length : 23/26

IRL : GTAAGCGGTAACTGACCGGCGTTATTCCAGGGCACGTGATCGACGTGGAC
IRR : GTAAGCGGTAACTGACGAGCGTTCTTGACTACTTCGAGATCCCGAAGAGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCGATGCCTT	CGTGCAGG	TGCTGCAAGT	8
CCTTGACCTC	GGTCCGGC	GGCTGCGGAT	8
AAGTTGATAC	GGGCTGCC	GATTGAAAGT	8

DNA sequence

GTAAGCGGTAACTGACCGGCGTTATTCCAGGGCACGTGATCGACGTGGACAATTGCCTCCAGGGTCAACAATGGAGGGATCGTTCATGGCAGGGGTGCTC
AAGCGCAAATGGCGCCGGCGCAGCCGAGAGGAGTGGCAGGAGGTGTTCGCCCGCCACGGTTCGAGCGGGCTGAGCGTCACGGCATTCTGCGCGCACGAGT
CGATCAGCGTCTCGAGCTTTCAGCGCTGGCGTGCGATCGTCGGGCCGGTGTGCGGCAAGGCCGTCGCGGTGGGGCCGGCTCGGCAAGAGGCGTTCGTCGA
TCTGGGAGTGCTGGGTTCGGGCGGCGCTTCGCGCTGGGAGTTGAAGCTTGACCTGGGTGACGGCGTGGTGCTGCACCTGGTGCGCGGCTGATGTTCTTTC
CCGAAGGCGCGGTGCGGGTGCATCTGTACGGGCGCCCGGTCGACATGCGCAAATCCTTCGACGGCTTGTATGCGCTGGCGCGCCACGGCGTGGGACAGGA
TCCGTTGTCGGGGCATCTGTTCGTGTTCATCAACCGGCGCGCGACCCAGATGAAGGTGTTGTACTGGGACCGCAGCGGGTTTTGCATCTGGGCCAAGCGG
CTCGAGTCGGGTCGCTTCGTGTCGGACTGGTCGCGGGCGACGAGCCGCGAGATGGACTGGACGGGGCTGAAGCTGTTGCTCGAAGGCATCGAGCCGGCAC
GCTTCAAAAAGCGCTTCGCGCTGCCCGCAAGTCACCGCAAACCCGCATGAATGCTGGCTTTTTTGGTAAAATGACGCCATGCCTCCGACGCCTTTGACGC
AGCTGCCGACCCGTGAAGAAGCCGCCCGGTGGACGGCCGATCAGGTCGTCGAACTGGCGGGCGCGCATCTGGCGCAGCAGCGCCAGTTCGAGGTCATCAA
AGGCGAATGCGAGGCGATGCGCCACCAGCTCGACTGGTTCCGGCGCCAGCTCTTCGGGGCGAAGAGCGAGAAGCGCCTCGTGGACGCCAACCCGCATCAG
ATGAGCCTGGGCGAGTTGCCGGTGCCCGAATCCTCGCCGCCGCCCCCCGGTCAGGACATTGCCGCCCACACCCGCCGGGCCCGCACCAGCGACTGCGCCA
AGGGCGACGCATCGGCGCTGTTCTTCGACGAGGCCCGCGTGCCGGTCGAGACCATCGAGGTGCCCAACCCCGAGGCCGAGGGGCTCGCCCCCGACCAGTT
CGAGGTGATCGGCGAAAAGGTCAGTCACCGCCTGGCGCAGCGCCCGGGCAGCTACGTGATCCTCAAGTACGTGCGTCCGGTGATCAAGCGCCTCGACACC
CAGGCGATTTCCTGCCCGGCGGCACCCGGGGGGGTGATTCGCGGCAGCCGCGCCGATGTGAGCTTCGTCGCCGGGCTCATCACCGACAAGTTCTGCTACC
ACCAGCCGCTCTACCGCCAGCACCAGCGGCTCGGCGACAATGGCATCAGGGTGTCGCGCCCGTGGCTCACGCAGCTCACCCACGGGGCGCTCGCGCTGCT
CGAACCGATCTTTACGGCCCAACTCGACTCGATCCGCGCGAGCCGCATCAAGGCGATGGACGAGACCCCGATCAAGGCCGGCCGTGCGGGCCCCGGCAAG
ATGAAGGGCGGCTACTTCTGGCCGGTCTATGGCGAACGTGACGAGATCTGTTTCGTCTATCGCGAGAGCCGCAAGGCGCAGCACATCGAGCAGATCCTGG
GCACCGACCCGCCGCCCGAGGGGGCGGTGCTGCTCACCGATGGCTATGGCGCCTACGAACGCTATGCCGAGAAGTGCGGGCTCACGCACGCGCAATGCTG
GGCGCACTCGAGGAGGAAGTTCTTCGACGCCCAGTCGGTCGAGCCCGAGCGCGCGGGCCGGGCGCTGGAGATGATCGGCAAGCTCTATGCGGTCGAAAAA
CGCATCCGCGAGGCCACGCTCGTCGGCGAGGCGTGCCGGGCGTACCGGATCGAGCATGCACAGCCCGTGGTCCACGAGTTCTTCGCCTGGGTCGATGCCC
AGTTCGACACCCACGGCCTGTTGCCCAGCTCGCCGCTCACCACCGCCATGGCTTATGTGCGGGAGCGCCGGGCCGCCCTCGAGGTGTACCTGCGCGATCC
TGAAGTGGCGATCGACACAAACCATCTCGAGCGGGCGCTACGCGTGGTGCCGATGGGTCGTCGCAACTGGCTCTTTTGCTGGACCGAGGTCGGCGCCAAA
TACGTCGGTATCGCGCAGAGCCTGATCGCCACCTGCCGGCTGCACGACATCGATCCGTACGACTATCTGGTCGATGTGCTGCAGCGTGTCGGCCAACACC
CCGCTGCCGACGTCGCGCAACTCACCCCCCGTCTGTGGAAGCAGCACTTCGCTGCCAACCCGCTGCGATCCGATCTCTTCGGGATCTCGAAGTAGTCAAG
AACGCTCGTCAGTTACCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
306 bp	101 aa	86	391	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MAGVLKRKWRRRSREEWQEVFARHGSSGLSVTAFCAHESISVSSFQRWRAIVGPVCGKAVAVGPARQEAFVDLGVLGSGGASRWELKLDLGDGVVLHLVR
G

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
360 bp	119 aa	391	750	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MFFPEGAVRVHLYGRPVDMRKSFDGLYALARHGVGQDPLSGHLFVFINRRATQMKVLYWDRSGFCIWAKRLESGRFVSDWSRATSREMDWTGLKLLLEGI
EPARFKKRFALPASHRKPA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1617 bp	538 aa	779	2395	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPPTPLTQLPTREEAARWTADQVVELAGAHLAQQRQFEVIKGECEAMRHQLDWFRRQLFGAKSEKRLVDANPHQMSLGELPVPESSPPPPGQDIAAHTRR
ARTSDCAKGDASALFFDEARVPVETIEVPNPEAEGLAPDQFEVIGEKVSHRLAQRPGSYVILKYVRPVIKRLDTQAISCPAAPGGVIRGSRADVSFVAGL
ITDKFCYHQPLYRQHQRLGDNGIRVSRPWLTQLTHGALALLEPIFTAQLDSIRASRIKAMDETPIKAGRAGPGKMKGGYFWPVYGERDEICFVYRESRKA
QHIEQILGTDPPPEGAVLLTDGYGAYERYAEKCGLTHAQCWAHSRRKFFDAQSVEPERAGRALEMIGKLYAVEKRIREATLVGEACRAYRIEHAQPVVHE
FFAWVDAQFDTHGLLPSSPLTTAMAYVRERRAALEVYLRDPEVAIDTNHLERALRVVPMGRRNWLFCWTEVGAKYVGIAQSLIATCRLHDIDPYDYLVDV
LQRVGQHPAADVAQLTPRLWKQHFAANPLRSDLFGISK

Blast result :

Comments

ISAzo19 is 56%(orfA), 79%(orfB), 73%(orfC) aa similar to ISCARN15.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)