ISAzba1

  • Family ISKra4
  • Group ISAzba1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
EU784144 ND Azospirillum brasilense
Azospirillum brasilense Sp245 85-MDa plasmid p85
DNA section
IS Length : 1465 bp

Ends


IR Length : 22/23

IRL : GAGACCGTCGCAAACCTGGGGGGAGAATGGTACGGCGCGCTCGGATGTCA
IRR : GAGACCGTCGCAAACTTGGGGGGCGTTACGCCGCCCGGCGAAATGGTCGG

Insertion site


Left flankDirect repeatRight flankDR Length
GCTGCTAAAGGAAGTCTCGTTC0

DNA sequence

GAGACCGTCGCAAACCTGGGGGGAGAATGGTACGGCGCGCTCGGATGTCAGCGAGTCGGGTGTACGATGAAGTGGCGGGTCATGGTTGAGGTGACGGGCG
AGGAGGGCGCGGTGACGCAGCATGTGATCTCCGAGGGTAAGCGCACCGGGGCCGGTCAGGCGGCGACGCTCGGGCTGAGTCTGAGCGAGGGCAAGGCGAC
CCTGGCCGGCCTGCAGCGCGTCCTGGTCATGGCCCAGGCCGACGCGCATTGTCGCCTTCGGCGTCGGTGCGGCCACTGCGGAGCGTCGCGCCCGCTCAAG
GACCACCGCTCCCGCCGGCTGGTGTCGTTGTTTGGCACGGTGGAGGTGCGCGCACCCCGTTTTAAGCCCTGTCGCTGCGGCGTGGCCTGCCGGCGCAGCC
TGACCCCGGTCGCCGAGATCATGCCCGACCGCTGCACCCCTGAGTACGAACGCATTCTATCCGGGATGGGCAGCGCGCTGCCCTATCGGCGGGCGCGGGC
GTTGCTGGCCGAGCTGCTGCCCTTGGATGCGGCTCCCGATGTGGAAACGGTCCGCCGGCGCACGCTCCAGGTCGGCGCCCGGCTCGAACGGGCGGCGCTG
GTCCCGCCGGACGAGGGTTCGTCGGCCCCGGCAACGGCGATCACGCTGGCCATCGACGCGGGGCATGTGAAGTCGATTAAGACTTATCAGGTGCGCTCGT
TCGAGGTGATGCTCGCGCGGGTGGGCAACGATCAGGGGCGGGAGGTCGTTTTCAGCAGCCTGCCGACCGAGGCCGACCGGCAAGTGCGGCAACTCCATCA
TGTTTTGCGCCGTCTGGGGGCGACGGCGACCACACCGGTCACCATCCTCAGCGACGGCGCAGAGGGTCCACGGTCGCTGGGCGCCGCGGCCAGCGTCGGC
CCGATCCGTAACGTCCTCGACTGGTTCCACCTATCGATGCGGGTCCAGCACGTGGCGCAGGCCGTCAAGGGCTGGGCCACGGCGGTGCCGGACGCCGCGG
AGCGGACGACGATGCTGGACGACGCCGTGACCCGCCTGCGTTGGCGGCTCTGGCACGGCCAGACCGAACGGGCGCTGACCTTGATCGGCGACCTCCTGAC
CGACCTCGACGCCGCTTTGGAAGCCGCTGACGACCCGCCGGCCCGGAAAGTGGCCGCGCTGCTGCGCGCCTTGGAGACGTACGTCGTCGGGCAAGCCGAC
CTGATCATCGACTACGCCGCCGCCCGGCATCGGGGCGAGGCCATTTCCACGGCGACGACCGAAAGCACCGTCCAACGTCTGCTGCACCGACGCATGGGCG
CCAATCAGCAGATGCGCTGGTCTCCGCGCGGTGCCCATCGGATGCTAAAGGTGCGCACCGCGGTGATGAACGGCACACTCGCCGCGGATCACGCCGCGGC
CGAGCCATACGCCCGCCGACCATTTCGCCGGGCGGCGTAACGCCCCCCAAGTTTGCGACGGTCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1374 bp457 aa671440+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKWRVMVEVTGEEGAVTQHVISEGKRTGAGQAATLGLSLSEGKATLAGLQRVLVMAQADAHCRLRRRCGHCGASRPLKDHRSRRLVSLFGTVEVRAPRFK
PCRCGVACRRSLTPVAEIMPDRCTPEYERILSGMGSALPYRRARALLAELLPLDAAPDVETVRRRTLQVGARLERAALVPPDEGSSAPATAITLAIDAGH
VKSIKTYQVRSFEVMLARVGNDQGREVVFSSLPTEADRQVRQLHHVLRRLGATATTPVTILSDGAEGPRSLGAAASVGPIRNVLDWFHLSMRVQHVAQAV
KGWATAVPDAAERTTMLDDAVTRLRWRLWHGQTERALTLIGDLLTDLDAALEAADDPPARKVAALLRALETYVVGQADLIIDYAAARHRGEAISTATTES
TVQRLLHRRMGANQQMRWSPRGAHRMLKVRTAVMNGTLAADHAAAEPYARRPFRRAA

 

Blast result :
Comments
References
1] Katsy, E.I. and Prilipov, A.G. (2008) Direct submission.
2] Katsy,E.I. and Prilipov,A.G. (2009) Plasmid 62 (1), 22-29