ISAva2
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007410 | ND | Anabaena variabilis | Anabaena variabilis ATCC 29413 Anabaena variabilis ATCC 29413 plasmid A Anabaena variabilis ATCC 29413 plasmid C |
DNA section
IS Length : 1547 bp
Ends
IR Length : 14
IRL : GTAAGGATTCAGGTAACAGAGTATTGACAAAGGGGACAGTAGAGGTGGAG
IRR : GTAAGGATTCAGGTCTAATATTGCGGAAGTAAAGAGGGGCGAGAGTGAGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTTTTTGAA | GAATCCAG | AAGTCAGAAT | 8 |
| AGTTTGAACT | TCATTCTT | AAGCTGTTGG | 8 |
| AAGATACAAT | AAATTTTC | TATATTTTGA | 8 |
| ATTCAATATA | GTTTCATA | TTTAATTCTT | 8 |
| AACTAAATTA | GTTAAGTC | TATCTTAGAT | 8 |
| TTGTCTGTAA | AAGTAATA | TGCTTTCGGT | 8 |
| AATCGGATTT | GTCAAATT | GTATTTTGAC | 8 |
| AATGTCAACA | CGATAAAG | TACAATCTAT | 8 |
| GAACTTCATA | ACCTTGTTC | TATGAGAAAT | 9 |
DNA sequence
GTAAGGATTCAGGTAACAGAGTATTGACAAAGGGGACAGTAGAGGTGGAGTATCAAAAATATGAACCAAAACTTGCCTCAAGATTTAGACAGAGAAAGTT
TGAGCCAGTTATCTAAAGAAGAACTGGTGGACATCATCATTGAGCAGAGCAAGGTAATACGTGAGCTACAAAAGATAATATTAGAACTACAGCAAGAAAT
AGAGCGTTTAAAAGTCAGCAGAGATTTAGATAGTTCTAATTCATCAAAACCTCCATCACAAGACATTCACAAAAAAAGCGAAAAAGAAAAAGTGCCTCAC
CTTGAGGAATCAAACCCACCGAAAAAGAAACCAGGTGGGCAACCAGGACATCAAGGTAAAACTCGAAAGGGTTTTGGCAGAGTAGATCGCTATGAAATTT
TACGTCCAACGGATTGCATTTACTGTGGTCAAAAAGCATTTGCACCCCTAGCAGTAAAAGTAGAAAAACACGCGGTAGCGCAACTAGTAGAATGTCCCAT
AGAAATAGTTGAGTATCAACGCCATACGTGCGTGTGTGAATGCTGTGGAAATATACAAACAGCAGCCTGGCCCCAAGAAATTATTCCAGGACAAGATTTA
GGAACGTCGTTACAAGCATTTTTAGGGTGGACAAATAACTATGCACATATGCCCTACGAAAAACAGCAGGAAATGCTTTGGGAACTTGGTGAGATTGAAA
TTGGATTGGGAACTTTAGTCGCCACCAATGAACGAATACAACAAGCAATTGAACCGAGTATTCATGAGTTAAGTAATTGGGTAAAACAGACACAACCTAA
CATCCATGTAGATGAAACACCTTGGTCAGTTAAAGGGGTTAAAGAATGGTTGTGGGTAGTGGCTAATTCTGAATTCTGCCTGTTTACTGCGGCTGATACT
CGTTCTAGAGCCGAACTAGAAGCAATTTTAGGGACTGAGTATACAGGTGTACTCAGCAGCGATGATTTTAGTGTTTATAATGGCTATCAAGCTGTTGCCC
AACAGAAATGTTTGGCTCATCTACGTCGTCACTTCAAAAAATTGATTCAACTTCCCGGTCTTCACAACAAAGCTATCGGCGAAACCTTTGTCAATTTAAT
TGATGAAGCCTTCAGAAGTTATGCTCAATGGTTTGAAACTCTTGACTCAAACAGTTATAACGATTGGATAAATCAATTCAAATTCAAGTTGCAATTTTCT
GTTGATCAATGGATTAACTTGGCAGGAGCTACGGCTGGAAACCTTTTACGTTCTTTGCGCGATAAAGCAAGCCAATGGTGGTATTTCCTTGACCATCCTG
AAGTTCCTCCTGATAATAATCTCGCTGAACGCTCGCTGCGTTTAGCTGTGACAAAACGTAAGGTTAGTGGTGGTTCACGCTCGATGAAGCGGTTTCAACA
TACTGCCAATTTGTTGACGGTAGTGCAGACTTGTCGCCGTCAAGGTAGGTCTGTTATTGATTTTTTTGTGCAAGCTCTAATTGCTGATTCTAATAATTCT
CACTCTCGCCCCTCTTTACTTCCGCAATATTAGACCTGAATCCTTAC
TGAGCCAGTTATCTAAAGAAGAACTGGTGGACATCATCATTGAGCAGAGCAAGGTAATACGTGAGCTACAAAAGATAATATTAGAACTACAGCAAGAAAT
AGAGCGTTTAAAAGTCAGCAGAGATTTAGATAGTTCTAATTCATCAAAACCTCCATCACAAGACATTCACAAAAAAAGCGAAAAAGAAAAAGTGCCTCAC
CTTGAGGAATCAAACCCACCGAAAAAGAAACCAGGTGGGCAACCAGGACATCAAGGTAAAACTCGAAAGGGTTTTGGCAGAGTAGATCGCTATGAAATTT
TACGTCCAACGGATTGCATTTACTGTGGTCAAAAAGCATTTGCACCCCTAGCAGTAAAAGTAGAAAAACACGCGGTAGCGCAACTAGTAGAATGTCCCAT
AGAAATAGTTGAGTATCAACGCCATACGTGCGTGTGTGAATGCTGTGGAAATATACAAACAGCAGCCTGGCCCCAAGAAATTATTCCAGGACAAGATTTA
GGAACGTCGTTACAAGCATTTTTAGGGTGGACAAATAACTATGCACATATGCCCTACGAAAAACAGCAGGAAATGCTTTGGGAACTTGGTGAGATTGAAA
TTGGATTGGGAACTTTAGTCGCCACCAATGAACGAATACAACAAGCAATTGAACCGAGTATTCATGAGTTAAGTAATTGGGTAAAACAGACACAACCTAA
CATCCATGTAGATGAAACACCTTGGTCAGTTAAAGGGGTTAAAGAATGGTTGTGGGTAGTGGCTAATTCTGAATTCTGCCTGTTTACTGCGGCTGATACT
CGTTCTAGAGCCGAACTAGAAGCAATTTTAGGGACTGAGTATACAGGTGTACTCAGCAGCGATGATTTTAGTGTTTATAATGGCTATCAAGCTGTTGCCC
AACAGAAATGTTTGGCTCATCTACGTCGTCACTTCAAAAAATTGATTCAACTTCCCGGTCTTCACAACAAAGCTATCGGCGAAACCTTTGTCAATTTAAT
TGATGAAGCCTTCAGAAGTTATGCTCAATGGTTTGAAACTCTTGACTCAAACAGTTATAACGATTGGATAAATCAATTCAAATTCAAGTTGCAATTTTCT
GTTGATCAATGGATTAACTTGGCAGGAGCTACGGCTGGAAACCTTTTACGTTCTTTGCGCGATAAAGCAAGCCAATGGTGGTATTTCCTTGACCATCCTG
AAGTTCCTCCTGATAATAATCTCGCTGAACGCTCGCTGCGTTTAGCTGTGACAAAACGTAAGGTTAGTGGTGGTTCACGCTCGATGAAGCGGTTTCAACA
TACTGCCAATTTGTTGACGGTAGTGCAGACTTGTCGCCGTCAAGGTAGGTCTGTTATTGATTTTTTTGTGCAAGCTCTAATTGCTGATTCTAATAATTCT
CACTCTCGCCCCTCTTTACTTCCGCAATATTAGACCTGAATCCTTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1473 bp | 490 aa | 61 | 1533 | + | No |
Chemistry : DDE
ORF sequence :
MNQNLPQDLDRESLSQLSKEELVDIIIEQSKVIRELQKIILELQQEIERLKVSRDLDSSNSSKPPSQDIHKKSEKEKVPHLEESNPPKKKPGGQPGHQGK
TRKGFGRVDRYEILRPTDCIYCGQKAFAPLAVKVEKHAVAQLVECPIEIVEYQRHTCVCECCGNIQTAAWPQEIIPGQDLGTSLQAFLGWTNNYAHMPYE
KQQEMLWELGEIEIGLGTLVATNERIQQAIEPSIHELSNWVKQTQPNIHVDETPWSVKGVKEWLWVVANSEFCLFTAADTRSRAELEAILGTEYTGVLSS
DDFSVYNGYQAVAQQKCLAHLRRHFKKLIQLPGLHNKAIGETFVNLIDEAFRSYAQWFETLDSNSYNDWINQFKFKLQFSVDQWINLAGATAGNLLRSLR
DKASQWWYFLDHPEVPPDNNLAERSLRLAVTKRKVSGGSRSMKRFQHTANLLTVVQTCRRQGRSVIDFFVQALIADSNNSHSRPSLLPQY
TRKGFGRVDRYEILRPTDCIYCGQKAFAPLAVKVEKHAVAQLVECPIEIVEYQRHTCVCECCGNIQTAAWPQEIIPGQDLGTSLQAFLGWTNNYAHMPYE
KQQEMLWELGEIEIGLGTLVATNERIQQAIEPSIHELSNWVKQTQPNIHVDETPWSVKGVKEWLWVVANSEFCLFTAADTRSRAELEAILGTEYTGVLSS
DDFSVYNGYQAVAQQKCLAHLRRHFKKLIQLPGLHNKAIGETFVNLIDEAFRSYAQWFETLDSNSYNDWINQFKFKLQFSVDQWINLAGATAGNLLRSLR
DKASQWWYFLDHPEVPPDNNLAERSLRLAVTKRKVSGGSRSMKRFQHTANLLTVVQTCRRQGRSVIDFFVQALIADSNNSHSRPSLLPQY
Blast result :
Comments
ISAva2 is 74% aa similar to ISCysp3.
References
1] ISfinder annotation (2008).
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Saunders,E.H., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K. and Richardson,P. (2005) Direct Submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Saunders,E.H., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K. and Richardson,P. (2005) Direct Submission GenBank.