ISAur1

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AEVG01000111 ND Actinobacillus ureae
Actinobacillus ureae ATCC 25976
DNA section
IS Length : 4947 bp

Ends


IR Length : 28/30

IRL : GGCTGTGTTTTAACAATGTGTTGATTTTTATACTAAATTTTGATAAAATT
IRR : GGCTGTGTTTTAACGATGTGTTGATATTTAATTTTAGAAAAGGTAGCTAT

Insertion site


Left flankDirect repeatRight flankDR Length
AAAGCGGTTAAAATTTTATTAAATTTTAAAGCAATCCGCCGCA0

DNA sequence

GGCTGTGTTTTAACAATGTGTTGATTTTTATACTAAATTTTGATAAAATTCCTTATAATGAATTGAATTATAATGGATTTTAGCATGTCGGGCGTAACAA
AAGAATTAGACATTTTAAAACAACTTTTTGAAAAGTTGTCCGATACGGATAAACAGGCTTTTTTGACTTCTGCCAGCTCAAAAGAACAGGTTAAAAAAGT
CATTGAACAAAAAAAAGTTACGAATTGCCCACATTGTCAATCTACGCATTTTGTAAAAAATGGTACGAAATGCAATAACCAACGCTATTTGTGTCGTGAT
TGCAAAAAATCTTTTGTTGAGCAAACAGGCACTATTCTCTACAACACGCAAAAAGACATTGAAGTTTGGGAAAAATACATTCATTGCATGATTGAAAAAT
ACCCACTCCGCAAATGTGCTGAAATTTGTGAAATCAATCTTGCTACTGCGTTTACTTGGCGGCACAAAATTTTAGACGCACTTCAAAACATGATGAATGA
AGTTGAGTTAGACGGCATTGTGCAAGCCGATGAAACTTATTCGACGATTTCTTACAAAGGACATCACAAGGATTTTCAACTGCCGCGCCCTGCTCACAAA
CGCGGGACAAGAGCCGGTAAGCGCGGCATTTCAAAAGAACAAGTCTGTGTTCCTTGCGGTATCGATTTAGACGGTAAATCTGTTGCTAAAATTAGCAATT
TGGGTAAGCAGTCTCTAAAAGGTTTACAAAAAGTTCTAAATGGCAAAGTTGCCAAAGATTCTGTATTCGTTACCGATTCTTTAAGACCGTATCAAAAATT
ATCGTTGGACATGAATTTAAGCCATATCCGCATTCCACGCGGTAAACGTGCTGTCGGTACATTCAACATTCAAACCGTCAACAGCTACCACAGCAGGCTG
AAAAACCTGATTGTCCACCGCTTCAAAGGTGTGGCAACCAAGTATCTGAATAATTACTTGGTGTATCACAATTTCGTGAATTTTGCCAAAGATACTTTGG
CGAATAAAGAAGCTGTTTTGCTGGATTTCATTCGCAAAACCATGTGCACAATTAAGTCGGCAGACGTGGCTAAACGTCCTGCTATTCCTGTGTAAATTAA
CTTGACTTTCAAATTTAAGGTGTAATCATGTGCCTACAAGCCTAGTTTAATCTTGTCTTTTCAAACTTCCAACACTTATTTGGAGGTTTGAAATGTCTAA
ACTTCAATATCAATTCAACGAAAAATCAAATCAAATTTTTTCTCCACTAAATAACAAACCGTTAACATCACTCCCGAGGAAGAAATACGTCAAAAATTTA
TTTGTGTGTTGGTAAATGAATATGGTTATTCACTTAATCAAATGGCACAAGAATTGAGTCTAACCAATTCCAGTCGTGGTACTGGGCGAGCCAGAGCGGA
TATTGTTATTTGGAAATCCGAGCAAGCCAAAAAAAATAACGAACACGCCGCCATTGTAATTGAATGCAAATCCGACAACGTAACCATTCAGCCCGAAGAC
TATTATCAAGGCTTAAATTATGCTACTTGGGCCGGGGCTAATTTTTTTGTTACTCATAACAGTAAAGAAACTCGCTATTTTCAAGTTTTCAAAGAAAAAA
TCCCTAAATACCTAGGTAAAGAATTATCAGAATTACCAACAGCAAAACAACTTGTTGATAGCAAAGTCATCGAAGAGATGCTCAAAGCTGAAAAAGTTTT
TGAAAAAGATGAATTTGCCAAGCTACTGCATCAATGCCACAGTATTATTCGTAATAATGACAAATTATCTCCCGAAGCCTCATTTGATGAAATTAGCAAA
ATTTTGTTTATGAAAATTATGTATGAACGAAATAAACAAAAAGGGAAGATATTTACCAAAGAAGAATTTTTAGAACAAGAAAGTCATTATGAAAAGAATA
TACGTCCGGTAAATGTACGAAGAAATGGACCTAAAGATGATATTTCTTATGTGACTTATTGGTTTGATGACACAAAAAATGCATATAAGGCAGAGCAATT
ATTTGAAGATGATGAGGTTATTAAGATTCGTAGAGAAAGTTTCTTAGCAATTCTGGAAAAACTACAAATATACAATCTTTCTAAAACATCTGATGATGTA
AAGGGCGTTGCTTTTGAGAAATTTTTAGGGACAACATTTCGTGGTGAGTTAGGACAATTTTTCACACCGCGCAGTGTGGTGGAATTTATGACCGAAATTT
TAGATCCACAAGAAGGAGAACGCGTATGCGATCCTTGTAGTGGTTCTGGTGGTTTTTTGATTAACGCCTTTGAATATATGCGGGAATCCATTCGACAAGA
TTTAGAAGAAGAAAAAGAATCCATAAAAAATCGCTATTTTGATGAAGCCTACGAGCAGGCAGATGAACAGCAAAAAGCCAATATAGAAGCTAAAGTTGAT
GGCTTATTTAGCGAACTAAATAGCGAGCTAGATTTAGACAACCCAAATAGCCGTTTGTACCAACTTTCGCACAATTGCATTTATGGAACAGACGCTAATC
CGAGAATGGCACGAGTATCCAAAATGAATATGATTATGCACGGCGATGGGCACGGTGGCGTACATCACAATGATGGTTTGTTGAATATAAATGGTATTTT
TGAAGAACGCTTTGATGTAATTTTAACTAATCCACCTTTCGGTTCTCGTGTAGCAAAAGATTTAAAATTAACTCAAGAAGATAGCCTAATTGACAAGCCA
CATTATAAAAATTGGAAAGCTAAATATGAGAATTATGAACGTATCGGAAATGAGCGAAAATTAGAAATTGAAGAAAATAAAGTCATTGTTGATAAATTTG
AAGTGAGTAAGTTTAGCACTTTAACCGAAGTGATGTTTATTGAACGCTGCCTGAAACTGTTACGCAAAGGCGGACGTATGGGCATTGTTTTGCCGAAAGG
CGTATTAAATAATGGCGATTTACAAAAAGTTCGACATTATTTTGAAAGTAAAGCCAAAATTATTTTAATCACGTCCATTCCGCAAGATGTATTTGTTGCA
TCAGGTGCAACCGTGAAGCCTAGTATTGTCTTTCTGAAACGCTTTACTGAAAGCGAAGAACAGGAATATTTAACCGCTAAAATGCAAGCTACCGCCGAAG
TAGAGCAAAAATACGCCCAGCGAAAAGCCAAACTTCAAGCAATTTTGGATAAAAAAGCGGATAAATTACCACCCAAAGCCACAGATGAACAAAAAACCGA
ACAAAATCGCTTAAAAGATGAATTGAAACAAGCGAAAAAACAGGCGAAAAATGAACTCAAACAGTTAGAAATCCAAATTAAAGATGAAATCCGTCAAGCA
ATTAAAACCAAGTTTGACTACCAAATTCCGCTTGCAGAAGTAGAAAAAGCAGGGATTGACAGTACAGGTGCAGCCATTGAAAACGATTTACCAAAGCTAA
AAGACGAATATACACAATATCGCAAAGCTAAAAGTATTTGGGAAAATCATACCGCCCATCATTACGAGTACACAGAAAACGGTGGTAAATTAGAACGCAC
TTTACGCACGTTTGCGGAAAGGGCGGAATAATGGGAACAAGCAATTTTCAAACAGCTTTTTTGCATTTTGTAGATTTTTCGCAATTCAATAACTGGAATG
TAAAACAATACGTTAATACAAATTTGCTGAAATCAAATTTCAAAATTGAATTTTTGGCAGAACACTTAATTGAACAAAATAACAAAATCAAGCCTTTTGA
TTTTCCTGAAAAAGATTTTGCGATTTTGGGTGTGGATAACAAAGTAGGAATTTTTGATGCCTATATTTCAAAAGGCAAAGAAATCAATCAGCCATACAAA
AAAATGGAAACAGGTTTTATTGCCTACAATCCTTACCGTATTAACGTAGGTTCAATCGGATTGAAAACCGAAAAACATCAGCACCAATATATTAGCCCAG
CTTATGTGGTATTCAGTTGTCAGACTACATTGCTGCCTGAATATTTATTTTTGGTTTTCAAAACGAATTTTTACAACCGTATTATCAGAGAAAATACAAC
AGGTTCGGTTCGGCAAAATTTGAGTTTTGATAATTTGATTAAAATGCAAATTCCCTTACCTGACATCAACACGCAAAAAGCATTAGCCCAAGCCTATCAA
GATAAAATGGCAAAGGCCGATGAATTGGAAAAACAAGCCAATCAAATAGATAGCGATATTGAGCAATATCTGTTTGAACAGCTTGGCATTGAAATTCAAC
AAACTCAAAAAGTACAAACTGGGAAATTGCAGTTTGTGAATTTTAGGGATTTGAATTTATGGGGAGTTGTCAGTCAAGATGCCATTACTGCTGAAACTAT
TTTTAAATCCAATCAATTTAAAAATAAACCTATTACAAACTTTTTTGAAATTAACCCAACTACACAAATTCCCAGTAACCAAATCATTAGCTTCATTCCA
ATGGCAAATGTTTCTGACATTTATGGAGAAATTTCCATTTACGACAAGCAAACATTAAAGCCCAACTACACTAAATTTAAAGAAAATGATTTAATTTGGG
CAAAAATTACACCGTGTATGGAAAATGGAAAATCTGCGATTGCAAGCAATTTAGAAAATGGTTTTGGATTTGGTTCAACGGAATTTCATGTTTTGCGAGC
CAAAAACAAAGATTTTTCTATTCATTTGCTACATTCGCTTTTGCGTACCAGCCATTTAAGAAAAATTGCGACTCAGTATTTTACAGGTTCAGCAGGTCAG
CAACGAGTTCCAAAATCATTTTTGAAAGCCTTAACTCTGCCTGTTTTAAACCTAGAAATTCAAACCAAAATTCTCACATATATTCAAACCCAAAAGCAGC
AGCAAAAAGATAGTTTAGCTACTGCTTCTGCATATCGTATTGAGGCGTTAATGGAATTTGAGAAAGCTATTTTTAAATAAGAAAAGGTAACTATTAAATA
GCTACCTTTTCTAAAATTAAATATCAACACATCGTTAAAACACAGCC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
1023 bp340 aa731095+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDFSMSGVTKELDILKQLFEKLSDTDKQAFLTSASSKEQVKKVIEQKKVTNCPHCQSTHFVKNGTKCNNQRYLCRDCKKSFVEQTGTILYNTQKDIEVWE
KYIHCMIEKYPLRKCAEICEINLATAFTWRHKILDALQNMMNEVELDGIVQADETYSTISYKGHHKDFQLPRPAHKRGTRAGKRGISKEQVCVPCGIDLD
GKSVAKISNLGKQSLKGLQKVLNGKVAKDSVFVTDSLRPYQKLSLDMNLSHIRIPRGKRAVGTFNIQTVNSYHSRLKNLIVHRFKGVATKYLNNYLVYHN
FVNFAKDTLANKEAVLLDFIRKTMCTIKSADVAKRPAIPV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
2226 bp741 aa13063531+No
ORF function : Passenger Gene
Annotation : N-6 DNA MethylaseDescription :

ORF sequence :

MLVNEYGYSLNQMAQELSLTNSSRGTGRARADIVIWKSEQAKKNNEHAAIVIECKSDNVTIQPEDYYQGLNYATWAGANFFVTHNSKETRYFQVFKEKIP
KYLGKELSELPTAKQLVDSKVIEEMLKAEKVFEKDEFAKLLHQCHSIIRNNDKLSPEASFDEISKILFMKIMYERNKQKGKIFTKEEFLEQESHYEKNIR
PVNVRRNGPKDDISYVTYWFDDTKNAYKAEQLFEDDEVIKIRRESFLAILEKLQIYNLSKTSDDVKGVAFEKFLGTTFRGELGQFFTPRSVVEFMTEILD
PQEGERVCDPCSGSGGFLINAFEYMRESIRQDLEEEKESIKNRYFDEAYEQADEQQKANIEAKVDGLFSELNSELDLDNPNSRLYQLSHNCIYGTDANPR
MARVSKMNMIMHGDGHGGVHHNDGLLNINGIFEERFDVILTNPPFGSRVAKDLKLTQEDSLIDKPHYKNWKAKYENYERIGNERKLEIEENKVIVDKFEV
SKFSTLTEVMFIERCLKLLRKGGRMGIVLPKGVLNNGDLQKVRHYFESKAKIILITSIPQDVFVASGATVKPSIVFLKRFTESEEQEYLTAKMQATAEVE
QKYAQRKAKLQAILDKKADKLPPKATDEQKTEQNRLKDELKQAKKQAKNELKQLEIQIKDEIRQAIKTKFDYQIPLAEVEKAGIDSTGAAIENDLPKLKD
EYTQYRKAKSIWENHTAHHYEYTENGGKLERTLRTFAERAE

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1350 bp449 aa35314880+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGTSNFQTAFLHFVDFSQFNNWNVKQYVNTNLLKSNFKIEFLAEHLIEQNNKIKPFDFPEKDFAILGVDNKVGIFDAYISKGKEINQPYKKMETGFIAYN
PYRINVGSIGLKTEKHQHQYISPAYVVFSCQTTLLPEYLFLVFKTNFYNRIIRENTTGSVRQNLSFDNLIKMQIPLPDINTQKALAQAYQDKMAKADELE
KQANQIDSDIEQYLFEQLGIEIQQTQKVQTGKLQFVNFRDLNLWGVVSQDAITAETIFKSNQFKNKPITNFFEINPTTQIPSNQIISFIPMANVSDIYGE
ISIYDKQTLKPNYTKFKENDLIWAKITPCMENGKSAIASNLENGFGFGSTEFHVLRAKNKDFSIHLLHSLLRTSHLRKIATQYFTGSAGQQRVPKSFLKA
LTLPVLNLEIQTKILTYIQTQKQQQKDSLATASAYRIEALMEFEKAIFK

 

Blast result :
Comments
ISAur1 is 64% (transposase) aa similar to ISCco2.
References
1] ISfinder annotation (2017)
2] Muzny,D., Qin,X., Deng,J., Jiang,H., Liu,Y., Qu,J., Song,X.-Z., Zhang,L., Thornton,R., Coyle,M., Francisco,L., Jackson,L., Javaid,M., Korchina,V., Kovar,C., Mata,R., Mathew,T., Ngo,R., Nguyen,L., Nguyen,N., Okwuonu,G., Ongeri,F., Pham,C., Simmons,D., Wilczek-Boney,K., Hale,W., Jakkamsetti,A., Pham,P., Ruth,R., San Lucas,F., Warren,J., Zhang,J., Zhao,Z., Zhou,C., Zhu,D., Lee,S., Bess,C., Blankenburg,K., Forbes,L., Fu,Q., Gubbala,S., Hirani,K., Jayaseelan,J.C., Lara,F., Munidasa,M., Palculict,T., Patil,S., Pu,L.-L., Saada,N., Tang,L., Weissenberger,G., Zhu,Y., Hemphill,L., Shang,Y., Youmans,B., Ayvaz,T., Ross,M., Santibanez,J., Aqrawi,P., Gross,S., Joshi,V., Fowler,G., Nazareth,L., Reid,J., Worley,K., Petrosino,J., Highlander,S. and Gibbs,R. (2011) Direct GenBank submission.