ISAtu6

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010929 ND Agrobacterium tumefaciens
Agrobacterium tumefaciens Ti plasmid pTiBo542
Agrobacterium tumefaciens
DNA section
IS Length : 2798 bp

Ends


IR Length : 23/24

IRL : GTGAGCGTCCGGTGAGCTGTTTTTGCTGATCGGGCAAATCAGGCGATGTT
IRR : GTGAGCGTCCGGCGAAAGCGTTTTCGGGTATCGAGAGATGGAAGCTATGA

Insertion site


Left flankDirect repeatRight flankDR Length
AGTAAATACCGCCAATGAGGTGCGCGAT8
CGAAAAGATAGTCTTTATCACCGCGCTT8
GTAGAGCGTTGTTCAGCGAAATACTAGC8

DNA sequence

GTGAGCGTCCGGTGAGCTGTTTTTGCTGATCGGGCAAATCAGGCGATGTTCTGGCATTCGAACCAGCATTAGTGCTGACATTAATACCAGAACAAAGAGG
TTTCATGGCTCGCATGGAGATCATGTCCGGTACCGAGCGCAGGCGGCGCTGGTCGGACGAGGCGAAGCTCAGGATATTGGCGGAAGCTGATGAACCCGGT
GCTCGCATTGGTGAGGTGGCGCGCCGGCATGACGTTCATCCTGGCCAGATCCGCTTGTGGCGGAGGTCGTTCAACTATGTGGATCGACCCACGGTGTTCC
TTCCAGTGGAAATCATGGAGGAGGCTGGCGTAAGTCAGGCGTCTTCGACGGTAACGAGGCCGGCGATCGTCGAGATATTGCTGCGGAACGGTCGGTGCCT
GAAGGTTCCTGCTGACGTTGAGTTGAAGCTGCTCGGTCCGCTGATCGCTTGCGTGGAGGCGGCATGATCGGGCCATCGGGCAATGTAAGGGTCTATCTGG
CCTGCGGAGTGACCGATATGCGGCGTGGCATTGATGGCCTATCGGCGCTGGTCGAGACGGTCGTGAAGGAGGCGCCGGGCTCGGGCGCGATTTTCGGCTT
CCGCGGAAAGCGCGCCGACCGGATCAAGCTTCTCTGGTGGGACGGCCAGGGGTTCTGCCTGTTCTACAAGATTTTGGAGCGTGGATACTTTCCCTGGCCG
ACAGCGAAAGAAGGTGTCGCGCACCTGACGCAGGCGCAGCTTTCCATGCTGGTTGAGGGGATTGATTGGCGCCGCCCGGCATGGACGTCCGCTCCTGGCC
GAACAGGATAAAAGCTATATTTTGCAAGGGCATCCGAGGCTTAACGCTGGCGCGTCAGAGGCAAATCGGCTAGATTTGCGGATATGGAAACAGCGCCGCT
GGACAGTCAGGACGAACTCACTGCTTTGCGCGCACTGGTCGCCGAACAGGCGGCGAAGCTTGAGAGCCAGGAAGCCGAGGTCATCAAGCGAGACTCCATC
ATAGGGCTTCTTCGCGCGCAACTGGAGCTTCTCCGACATCGGCAGCATGGCGCCTCTTCGGAAAAGATCGACCGGAAGATAGAGCAGTTCGAACTGATGC
TGGAGGAGATTGAGGCTTCCCGTGCCGAGGCTGAACTGCGCTCCGGGAAAACACCCTTGCCGGATTTGGACGACGCGCCGGACAAGCCGAAGCGCAAACC
ATTGCCCGATGGTCTCGCCACCGAAGAGCTGATCTATGCGGCTCCCTGTAATTGCCCGACCTGCGGTGGCACCTCGTTCCTGAAGGCGCCCGACAGGGTG
GTTCAGGTGCTGGAACACGTGCCGGCGTCGGTCAAGATTGTCCGCCATGTCGAGAAGCGTATGATCTGCAAGGAATGCGATACGACAGTGGCTGGCGAGA
TGCCGACCTTGCCGATCGAGCGCGGCAAGCCCGGGCCTGGATTGCTCGCCCATATCATGGTCTCCAAGTTCGATGATCACATTCCGCTTTACCGTCTCTC
AGAGATGTATGATCGGCTGGGAATAGACATATCGCGCTCCGTGATGGCCGACTGGGTCGGCCGCGTATCCGCTTTGCTGACACCCATCGTCTTGTTGATC
AGGGCCCACATCGCCGCGCTTGACCGAATACATACGGACGATACCCCGGTCGATGTTCTCGACCCCGGACGGGGCAAGACAAAAACCGGCAGGGTCTGGG
TCTACGTCTTCGACGGCAGTGGCTATCAAGCCACCACTCCGGCAGCCATCGCCTATTACTACAGTCCTGATCGAAAGGGCACACATCCGGCTGACCACCT
GGCAAGCTTTAGCGGCGTCATGCATGCCGACGGTTATGGCGGCTACAGACAACTCTACGGCAACCAGATCGTTGAGGCCGCCTGCATGGCGCATGTACGT
CGCAAGTTCCATGACGTGATCAAGCTGAAGCCATCGCCGATCGCCGACGAAGCGCTGTCGCGCATCGGCGCGCTCTACGATATCGAGGATCGTATCCGCG
GCATGTCGGCTGATGAGCGTCGTACCCTGCGCCAACACCACGCCAGACCCATTCTGGACGAGCTGAAGACCTGGATCGAGGCGACACTCTCAACTTTGCC
ACAGAAGCAGAAGCTGGCCGAGGCAATGCGATATGCGCTGTCTCGATGGGCAGCCTTGAGCGTTTACATCGACGATGGCCGTGTCGAAATCGATAACAAC
ATAGCTGAGCGAGCGATGCGTCCGCTGGGCCTCGGCAGAAAAAACTGGTTATTCGCAGGCTCGGACAAGGGCGGTGAGCGCATCGCCAACATCCTGACCA
TCATCGAAACGGTCAAACTGCACGGCCATAATCCGGAGCTCTACCTGACAGATGTCCTGACCCGGATCCAGGATCACCCCAAAGACCGATTTGAAGATCT
GCTGCCCTGGAACTGGATGCCAGCAAAAGCTCGATGCGAGGCCGCCTGATGGCTCGCTCGAGGTTCATCTATACGCTCAGCCAAGTCGCCGGCATGATCG
GCGAAAACCTCGAACTGATCGAAGAAGTAACCGCAAACCCGGACAACATCTCCGAGGGCGAACTGGTTTACGTCAGCGATGGCAGTGAAGATGGCACGAA
GGGTCTGACCGGGAATGGCATCGAAGAACTTCAAAGCCTACTTGCCGACATCAGGACGTGGGACGGCGGTATCCGCGAGTTCCTCATCGACACGCAGTGC
GATCCTGAAATGATCGACCGCGTCATGGCCGATGAAATGAAACGCGGCTCATAGCTTCCATCTCTCGATACCCGAAAACGCTTTCGCCGGACGCTCAC
Protein section
ORF number : 4

 

ORF 1
LengthBeginEndStrandFusion ORF
354 bp117 aa114467+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MEIMSGTERRRRWSDEAKLRILAEADEPGARIGEVARRHDVHPGQIRLWRRSFNYVDRPTVFLPVEIMEEAGVSQASSTVTRPAIVEILLRNGRCLKVPA
DVELKLLGPLIACVEAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa464811+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIGPSGNVRVYLACGVTDMRRGIDGLSALVETVVKEAPGSGAIFGFRGKRADRIKLLWWDGQGFCLFYKILERGYFPWPTAKEGVAHLTQAQLSMLVEGI
DWRRPAWTSAPGRTG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1566 bp521 aa8842449+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

METAPLDSQDELTALRALVAEQAAKLESQEAEVIKRDSIIGLLRAQLELLRHRQHGASSEKIDRKIEQFELMLEEIEASRAEAELRSGKTPLPDLDDAPD
KPKRKPLPDGLATEELIYAAPCNCPTCGGTSFLKAPDRVVQVLEHVPASVKIVRHVEKRMICKECDTTVAGEMPTLPIERGKPGPGLLAHIMVSKFDDHI
PLYRLSEMYDRLGIDISRSVMADWVGRVSALLTPIVLLIRAHIAALDRIHTDDTPVDVLDPGRGKTKTGRVWVYVFDGSGYQATTPAAIAYYYSPDRKGT
HPADHLASFSGVMHADGYGGYRQLYGNQIVEAACMAHVRRKFHDVIKLKPSPIADEALSRIGALYDIEDRIRGMSADERRTLRQHHARPILDELKTWIEA
TLSTLPQKQKLAEAMRYALSRWAALSVYIDDGRVEIDNNIAERAMRPLGLGRKNWLFAGSDKGGERIANILTIIETVKLHGHNPELYLTDVLTRIQDHPK
DRFEDLLPWNWMPAKARCEAA

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
306 bp101 aa24492754+No
ORF function : Passenger Gene
Annotation : hypothetical proteinDescription :

ORF sequence :

MARSRFIYTLSQVAGMIGENLELIEEVTANPDNISEGELVYVSDGSEDGTKGLTGNGIEELQSLLADIRTWDGGIREFLIDTQCDPEMIDRVMADEMKRG
S

 

Blast result :
Comments
ISAtu6 orfA is 50% aa similar to ISPpa5_aa1.
ISAtu6 orfB is 77% aa similar to ISPpa5_aa2.
ISAtu6 orfC is 66% aa similar to ISCARN48_aa3.
ISAtu6 orfD is 55% aa similar to ISPpa5_aa4.

ISAtu6 was reconstructed by deletion of ISAtu7 sequence (family IS110).
References
1] Oger,P.M., Farrand,S.K., Olsen,G.J. and Reich,C.(2005) Direct submission GenBank.
2] ISfinder annotation (2009)