ISArch18
- Family IS1
- Group ISMhu11
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY714866 | ND | Uncultured archaeon | Uncultured archaeon GZfos36D8 |
DNA section
IS Length : 1107 bp
Ends
IR Length : 19/26
IRL : GGTAATGTCCCAACACGTAGTTGATATATAGGTGGATTACCTACCTGTAT
IRR : GGTAGTGTCCTAATTAATAGTTGGTAGTATCTTGAACATCAACAATTCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAAAACGAGGGCAT | GAAAAAAC | AAGGACAGCATG | 8 |
DNA sequence
GGTAATGTCCCAACACGTAGTTGATATATAGGTGGATTACCTACCTGTATTTATGACAAATAACAAATTAGAGGCAAATCCAGAAAAAGAGTTTTGCTCC
GATCCAAACTGCCGTGATTACGGCAAACGGGGTGAGGGCAATATAGTTAGATATGGACACGACAAAAATGGCAGGCAGCGATTTAAATGCAAGACATGCG
GAAAAGTCTTTGTAGAAACCAAAAACACGGTCTTCTACAATCGCAAGTTATCAGAGGATCAGATAATCCTTATCTGCAAATTGCTGGTCGAGAAAAATGG
AATAAGAGCCATTGAACGCATAATGGAGATCCACCGTGATACGATATCAGATGTCGTAAAAGACTTGGCAAGACATGCAAGGGAAGTGACGGACTTTTTG
ATACGGAATGTTGGACTAACCAAAGTCCAGGTAGATGAGCTGTGGTCATTCGTAAAAAAAAACAAAAGAACCTTGCCGGAGGAGATGGTGACGATGATGG
ACAGGGCGATTGCTGGATATACATAGCGAAGAAAGCAGATACAAAGCTTCATTTAGCGCATAGTACTGGTAAAAGAGTGCAAGACACGGCAGATGAACTA
ATGAAAACGGTGAGAAAACGGGGCAAGAAACCAACAAAGGATGAGAAGGCTACTTTCGCCAGTGACGGTAACGTTCAGTACACCAGTGCCATCCTGGAGA
ACTTCGATGTGGAAGCGATCAATTACGGACAACTCGTCAAAGAAAGGGAAGGGGGCAGAGTGGTTGGTAAGACCAGAACAATCATTTTCGGTGAGGTAGA
TGATGTGGACATAGATACGGTCTACATAGAGAGGTACAACCTAACGTTGAGACACGGCATTTCGAGGCTGGTCAGGAAAAGCCTATGCTTTTCTAAGTGT
AAGGGGATGTTGGACAACCATCTGGACGTGTATCAGTGCTATAACAACCTGATCAGGGTTAATTCGGCATTAACGATCAAAACGGAGAAAGGTGAGAAAA
ACATCGTTAGAACACCATGCATAGCTGAGGGCATCACGAATCACATTTGGACATGGGAGGAATTGTTGATGTTCAAGATACTACCAACTATTAATTAGGA
CACTACC
GATCCAAACTGCCGTGATTACGGCAAACGGGGTGAGGGCAATATAGTTAGATATGGACACGACAAAAATGGCAGGCAGCGATTTAAATGCAAGACATGCG
GAAAAGTCTTTGTAGAAACCAAAAACACGGTCTTCTACAATCGCAAGTTATCAGAGGATCAGATAATCCTTATCTGCAAATTGCTGGTCGAGAAAAATGG
AATAAGAGCCATTGAACGCATAATGGAGATCCACCGTGATACGATATCAGATGTCGTAAAAGACTTGGCAAGACATGCAAGGGAAGTGACGGACTTTTTG
ATACGGAATGTTGGACTAACCAAAGTCCAGGTAGATGAGCTGTGGTCATTCGTAAAAAAAAACAAAAGAACCTTGCCGGAGGAGATGGTGACGATGATGG
ACAGGGCGATTGCTGGATATACATAGCGAAGAAAGCAGATACAAAGCTTCATTTAGCGCATAGTACTGGTAAAAGAGTGCAAGACACGGCAGATGAACTA
ATGAAAACGGTGAGAAAACGGGGCAAGAAACCAACAAAGGATGAGAAGGCTACTTTCGCCAGTGACGGTAACGTTCAGTACACCAGTGCCATCCTGGAGA
ACTTCGATGTGGAAGCGATCAATTACGGACAACTCGTCAAAGAAAGGGAAGGGGGCAGAGTGGTTGGTAAGACCAGAACAATCATTTTCGGTGAGGTAGA
TGATGTGGACATAGATACGGTCTACATAGAGAGGTACAACCTAACGTTGAGACACGGCATTTCGAGGCTGGTCAGGAAAAGCCTATGCTTTTCTAAGTGT
AAGGGGATGTTGGACAACCATCTGGACGTGTATCAGTGCTATAACAACCTGATCAGGGTTAATTCGGCATTAACGATCAAAACGGAGAAAGGTGAGAAAA
ACATCGTTAGAACACCATGCATAGCTGAGGGCATCACGAATCACATTTGGACATGGGAGGAATTGTTGATGTTCAAGATACTACCAACTATTAATTAGGA
CACTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 474 bp | 157 aa | 53 | 526 | + | No |
Description : First part of the transposase
ORF sequence :
MTNNKLEANPEKEFCSDPNCRDYGKRGEGNIVRYGHDKNGRQRFKCKTCGKVFVETKNTVFYNRKLSEDQIILICKLLVEKNGIRAIERIMEIHRDTISD
VVKDLARHAREVTDFLIRNVGLTKVQVDELWSFVKKNKRTLPEEMVTMMDRAIAGYT
VVKDLARHAREVTDFLIRNVGLTKVQVDELWSFVKKNKRTLPEEMVTMMDRAIAGYT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 660 bp | 219 aa | 439 | 1098 | + | No |
Description : Second part of the transposase
ORF sequence :
AVVIRKKKQKNLAGGDGDDDGQGDCWIYIAKKADTKLHLAHSTGKRVQDTADELMKTVRKRGKKPTKDEKATFASDGNVQYTSAILENFDVEAINYGQLV
KEREGGRVVGKTRTIIFGEVDDVDIDTVYIERYNLTLRHGISRLVRKSLCFSKCKGMLDNHLDVYQCYNNLIRVNSALTIKTEKGEKNIVRTPCIAEGIT
NHIWTWEELLMFKILPTIN
KEREGGRVVGKTRTIIFGEVDDVDIDTVYIERYNLTLRHGISRLVRKSLCFSKCKGMLDNHLDVYQCYNNLIRVNSALTIKTEKGEKNIVRTPCIAEGIT
NHIWTWEELLMFKILPTIN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1046 bp | 348 aa | 53 | 1098 | + | Yes |
Chemistry : DDE
ORF sequence :
MTNNKLEANPEKEFCSDPNCRDYGKRGEGNIVRYGHDKNGRQRFKCKTCGKVFVETKNTVFYNRKLSEDQIILICKLLVEKNGIRAIERIMEIHRDTISD
VVKDLARHAREVTDFLIRNVGLTKVQVDELWSFVKKKQKNLAGGDGDDDGQGDCWIYIAKKADTKLHLAHSTGKRVQDTADELMKTVRKRGKKPTKDEKA
TFASDGNVQYTSAILENFDVEAINYGQLVKEREGGRVVGKTRTIIFGEVDDVDIDTVYIERYNLTLRHGISRLVRKSLCFSKCKGMLDNHLDVYQCYNNL
IRVNSALTIKTEKGEKNIVRTPCIAEGITNHIWTWEELLMFKILPTIN
VVKDLARHAREVTDFLIRNVGLTKVQVDELWSFVKKKQKNLAGGDGDDDGQGDCWIYIAKKADTKLHLAHSTGKRVQDTADELMKTVRKRGKKPTKDEKA
TFASDGNVQYTSAILENFDVEAINYGQLVKEREGGRVVGKTRTIIFGEVDDVDIDTVYIERYNLTLRHGISRLVRKSLCFSKCKGMLDNHLDVYQCYNNL
IRVNSALTIKTEKGEKNIVRTPCIAEGITNHIWTWEELLMFKILPTIN
Blast result :
Comments
ISArch18 is 53% aa similar to ISMhu11.
References
1] Hallam,S.J., Putnam,N., Preston,C.M., Detter,J.C., Rokhsar,D., Richardson,P.M. and DeLong,E.F. (2004) Science 305 (5689), 1457-1462