IS982
- Family IS982
- Group
Isoform Synonym(s) IS982A
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| L34754 | ND | Lactococcus lactis | Lactococcus lactis subsp. lactis KR2 plasmid pKR223 Lactococcus lactis subsp. cremoris SK11 plasmid pSK11L Lactococcus lactis subsp. cremoris Wg2 plasmid pAM1 |
DNA section
IS Length : 999 bp
Ends
IR Length : 19/20
IRL : ACCCGAATTGCTAGTTAATTTCATTGGAAAATAAATAAGTCGTGCTATCC
IRR : ACCCGAATTGCTAGTTGATTATTTAGCCATGACTTGATACCCGATAGAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAATATTTAC | TATCATT | ATAACAGATG | 7 |
DNA sequence
ACCCGAATTGCTAGTTAATTTCATTGGAAAATAAATAAGTCGTGCTATCCTAATCTTAAACCACTAAGCATTAGAAAGCGCACGACTTATATGACTTATA
ATAGCACACTCCCAAAAGTTTTTGTTTATTTACTGACAACCATTGAGACGCTTTATCAAACGAGGGTTCCCCTTGAGGTTCAAAACCGAAAGAACGTCCA
TCTCGCAACATCAGGTTGCTTAGTTATCGCTTGTTACCTCTGGGGCGTACTGCATTTTAGTGAAACGCTTAAAGCTAAGCACCAACTTGCTCAAAGTTTA
TTTCCTAATTTCCTAGAATATTCTCGCTTTGTCCGCCGTTGTAATGGCCTCTTACCGAGTATCCAAGTCATTCGCCAAGCACTCGTCTTTAAAGAGGTTG
AAGGAATGAGTGTATCCATTATTGACAGCTTCCCCATTCCTTTGTGTCAGCCTATTCGTAATTTCAGAAGCAAAGGTCTTGGAGATTATGCAAATGTTGG
CTACAATGCTACAAAGGGACAGTACTTCTATGGATGTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCA
ATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGC
TGGAATTAGAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAACATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGA
GCGAGTTTTCTCTTTTTTGACAAATCTAGGAGCTGAGCGTTGTAAAAGTCTTTCGCCTCAAGGTTTTCAATTGAAATTAGAGATGATACTTTTAGCGTAT
TCTTTACTGTTAAAATCAGCTAAATCACTGGAACCAGAGACTTTAAGATATTCTATCGGGTATCAAGTCATGGCTAAATAATCAACTAGCAATTCGGGT
ATAGCACACTCCCAAAAGTTTTTGTTTATTTACTGACAACCATTGAGACGCTTTATCAAACGAGGGTTCCCCTTGAGGTTCAAAACCGAAAGAACGTCCA
TCTCGCAACATCAGGTTGCTTAGTTATCGCTTGTTACCTCTGGGGCGTACTGCATTTTAGTGAAACGCTTAAAGCTAAGCACCAACTTGCTCAAAGTTTA
TTTCCTAATTTCCTAGAATATTCTCGCTTTGTCCGCCGTTGTAATGGCCTCTTACCGAGTATCCAAGTCATTCGCCAAGCACTCGTCTTTAAAGAGGTTG
AAGGAATGAGTGTATCCATTATTGACAGCTTCCCCATTCCTTTGTGTCAGCCTATTCGTAATTTCAGAAGCAAAGGTCTTGGAGATTATGCAAATGTTGG
CTACAATGCTACAAAGGGACAGTACTTCTATGGATGTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCA
ATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGC
TGGAATTAGAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAACATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGA
GCGAGTTTTCTCTTTTTTGACAAATCTAGGAGCTGAGCGTTGTAAAAGTCTTTCGCCTCAAGGTTTTCAATTGAAATTAGAGATGATACTTTTAGCGTAT
TCTTTACTGTTAAAATCAGCTAAATCACTGGAACCAGAGACTTTAAGATATTCTATCGGGTATCAAGTCATGGCTAAATAATCAACTAGCAATTCGGGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 91 | 981 | + | No |
Chemistry : DDE
ORF sequence :
MTYNSTLPKVFVYLLTTIETLYQTRVPLEVQNRKNVHLATSGCLVIACYLWGVLHFSETLKAKHQLAQSLFPNFLEYSRFVRRCNGLLPSIQVIRQALVF
KEVEGMSVSIIDSFPIPLCQPIRNFRSKGLGDYANVGYNATKGQYFYGCKCHALVSESGYVIDYTITPASMADSSMTEEVLSQFGTPTVLGDMGYLGQSL
HDRLELEGIDLMTPVRKNMKQKNILFPNFSKRRKVIERVFSFLTNLGAERCKSLSPQGFQLKLEMILLAYSLLLKSAKSLEPETLRYSIGYQVMAK
KEVEGMSVSIIDSFPIPLCQPIRNFRSKGLGDYANVGYNATKGQYFYGCKCHALVSESGYVIDYTITPASMADSSMTEEVLSQFGTPTVLGDMGYLGQSL
HDRLELEGIDLMTPVRKNMKQKNILFPNFSKRRKVIERVFSFLTNLGAERCKSLSPQGFQLKLEMILLAYSLLLKSAKSLEPETLRYSIGYQVMAK
Blast result :
Comments
IS982 is located between the ori and the opp (oligopeptide permease) gene cluster on the lactose plasmid pSK11L of Lactococcus lactis subsp. cremoris SK11. There are three copies of IS982 on pSK11L. The limits of IS982 are based on the comparison between all the other IS982 family members. These assumptions remain to be confirmed experimentally. A nearly identical copy is also present on the pAM1 plasmid from Lactococcus lactis subsp. cremoris Wg2. Closely related, but shorter sequences have also been found associated to the nisin-resistance (nsr) gene in Lactococcus lactis subsp. lactis biovar diacetylactis DRC3, as well as in Lactococcus lactis subsp. lactis ME2, downstream of the abiC gene. IS982 hybridizes with DNA of 6 Lactococcus lactis subsp. cremoris, 4 Lactococcus lactis subsp. lactis and 2 Lactococcus lactis subsp. lactis biovar diacetylactis.
References
1] Yu, W., Mierau, I., Mars, A., Johnson, E., Dunny, G., and McKay, L.L. (1995) Plasmid 33, 218-225.
2] Twomey, D.P., McKay, L.L, and O'Sullivan, D.J. (1998). J.of Bacteriology, 180 (22), 5844-5854.
2] Twomey, D.P., McKay, L.L, and O'Sullivan, D.J. (1998). J.of Bacteriology, 180 (22), 5844-5854.