IS91
- Family IS91
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X17114 | Y | Escherichia coli | Escherichia coli EC185 plasmid pSU316 Escherichia coli EC185 plasmid pSU233 |
DNA section
IS Length : 1829 bp
Ends
oriIS : CGAGTAGGCAGCCTGGCGGCTGCGGCTTGTCATGGTCTGGAATTACCGTTATAAAAAAAGATAATGTCATTGTCTTTCAGGTAGTTATATGGCCCGTTCA II struct. : No
terIS : TTAATAGTCGTTTCCGCTCATATGGTGCACAAGGGGTGTTGAAGAAACATCCGTTTTGTGGTGCTTTTTTAGTCTTTTGGGGATTTAAATTCCTATCGAT II struct. : No
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
DNA sequence
CGAGTAGGCAGCCTGGCGGCTGCGGCTTGTCATGGTCTGGAATTACCGTTATAAAAAAAGATAATGTCATTGTCTTTCAGGTAGTTATATGGCCCGTTCA
GCTAAACCCCGTAAACGCAAACCCGCACCACAAAGAAGCAAACTTCCCCGCTATGTTGTGAAACTTCATCCGGATGATTTTTTTGACGAAGAAGACGCTG
AAGTTCTGCGCTTTGATAATTTTGACGATGCCGTTGAGTGCTGCGCAGACCTGAATATTCCCTTCTTTGTGGATGCCGGAAACAAAAAGCTGGTCTTCTG
GTTTGTACGTGTTGATGACGAAGGGTATCCTGAAATAGCCCGCTGCACGGAGCGGGAGTTTGCGACCATTCTTGCCGGTATCAGCGCCGGCGGCATGTAC
TGCCCGGAGTGTGGCACGGTTCACTGGCCGGACGGAGTCCCCCCGCCTTCTGATGCTTCCCCGTTTTGCCGACATTTTTCAGCAGGGTAACCGCTGGCTT
AACTGGCTGGAGAAACAGCCGGAAGGTTCAGTGCGTCCGGTGGTGACTGAGTCAGTGACAAAAATCATGGCATGCGGGACCACGCTGATGGGCTACACGC
AATGGTGCTGTTCGTCACCGGACTGTTGCCACACCAAAAAGGTCTGCTTCCGGTGTAAAAGCCGCTCCTGTCCGCACTGCGGGGTGAAGGCTGGCGCACA
GTGGATACAGTATCTGCTGAGCCTGGTCCCCGACTGCCCGTGGCAGCATATTGTGTTCACACTTCCCTGCCAGTACTGGTCCCTGGTGTTCCACAACCGG
TGGTTACTGGCAGAGATGAGCCGCATTGCAGCGGATGTGATACTGGAAATCTGCCATCAGACAGATGTGGAGCCGGGGATATTCACGGTGATCCACACAT
GGGGGCGTGACCAGCAGTGGCATCCGCATATCCATTTATCGACAACTGCCGGTGGTGTGACGTCGGGCCACACCTGGAAAAATCTTCATTTTTACGCCCG
TAAGGTGATGAGCATGTGGCGTTACCGGATAACGCGGCTACTGTCCCGGAAATACCCGGAGCTGGTGATACCGGATGAACTGGCAGTGGAAGGAAACAGC
AAACGGGACTGGAATTGCTTCCTGGACACGCATTACCGCCGCGGCTGGAATGTCAACATATCCAGGGTGATGGATAACGCCACACATGTGGCGGTGTACT
TCGGCTCTTACCTGAAAAAGCCACCGGTGCCGATGAGTCGGCTGGAGCATTATGCCGGTCAGGATGAAATCGGTCTGCGTTACAACAGTCACCGTACAAA
ACGGGAAGAATACCTGTTGATGAGTGGAGATGAGTTCATGGAAAGGTTCTCCTGGCATGTAGCAGATAAGGGGTTCCGTATGGTGAGGTACTACGGTTTC
CTGAGTCCGGTGAAGCGCCGGTTACTGGAAGAAGTTGTGTACGTCATAACGGAGACGGTGAGAAAAACGGCGATGCAAATCAGGTGGAGAGGGATGTATC
AGCGGTTACTGAAGGTTGACCCGCTGAAGTGCATTCTGTGCGGAAGTCAGATGCGTTTTACGGGGCTGAAGCGGGGTTACCGACTGGCAGAGCTGGTCCT
GATGCATGAGCGACTTGGCACGACAGCAGGTGTGCGGCTGAGAGCCGCAGAGGGGAAGTTGCGTCCATTTTACCGGAAACGGAGCAAAAAACCGCCATTC
ATACCCTGTATCAATCAGTGTCATCCTGTTTAATAGTCGTTTCCGCTCATATGGTGCACAAGGGGTGTTGAAGAAACATCCGTTTTGTGGTGCTTTTTTA
GTCTTTTGGGGATTTAAATTCCTATCGAT
GCTAAACCCCGTAAACGCAAACCCGCACCACAAAGAAGCAAACTTCCCCGCTATGTTGTGAAACTTCATCCGGATGATTTTTTTGACGAAGAAGACGCTG
AAGTTCTGCGCTTTGATAATTTTGACGATGCCGTTGAGTGCTGCGCAGACCTGAATATTCCCTTCTTTGTGGATGCCGGAAACAAAAAGCTGGTCTTCTG
GTTTGTACGTGTTGATGACGAAGGGTATCCTGAAATAGCCCGCTGCACGGAGCGGGAGTTTGCGACCATTCTTGCCGGTATCAGCGCCGGCGGCATGTAC
TGCCCGGAGTGTGGCACGGTTCACTGGCCGGACGGAGTCCCCCCGCCTTCTGATGCTTCCCCGTTTTGCCGACATTTTTCAGCAGGGTAACCGCTGGCTT
AACTGGCTGGAGAAACAGCCGGAAGGTTCAGTGCGTCCGGTGGTGACTGAGTCAGTGACAAAAATCATGGCATGCGGGACCACGCTGATGGGCTACACGC
AATGGTGCTGTTCGTCACCGGACTGTTGCCACACCAAAAAGGTCTGCTTCCGGTGTAAAAGCCGCTCCTGTCCGCACTGCGGGGTGAAGGCTGGCGCACA
GTGGATACAGTATCTGCTGAGCCTGGTCCCCGACTGCCCGTGGCAGCATATTGTGTTCACACTTCCCTGCCAGTACTGGTCCCTGGTGTTCCACAACCGG
TGGTTACTGGCAGAGATGAGCCGCATTGCAGCGGATGTGATACTGGAAATCTGCCATCAGACAGATGTGGAGCCGGGGATATTCACGGTGATCCACACAT
GGGGGCGTGACCAGCAGTGGCATCCGCATATCCATTTATCGACAACTGCCGGTGGTGTGACGTCGGGCCACACCTGGAAAAATCTTCATTTTTACGCCCG
TAAGGTGATGAGCATGTGGCGTTACCGGATAACGCGGCTACTGTCCCGGAAATACCCGGAGCTGGTGATACCGGATGAACTGGCAGTGGAAGGAAACAGC
AAACGGGACTGGAATTGCTTCCTGGACACGCATTACCGCCGCGGCTGGAATGTCAACATATCCAGGGTGATGGATAACGCCACACATGTGGCGGTGTACT
TCGGCTCTTACCTGAAAAAGCCACCGGTGCCGATGAGTCGGCTGGAGCATTATGCCGGTCAGGATGAAATCGGTCTGCGTTACAACAGTCACCGTACAAA
ACGGGAAGAATACCTGTTGATGAGTGGAGATGAGTTCATGGAAAGGTTCTCCTGGCATGTAGCAGATAAGGGGTTCCGTATGGTGAGGTACTACGGTTTC
CTGAGTCCGGTGAAGCGCCGGTTACTGGAAGAAGTTGTGTACGTCATAACGGAGACGGTGAGAAAAACGGCGATGCAAATCAGGTGGAGAGGGATGTATC
AGCGGTTACTGAAGGTTGACCCGCTGAAGTGCATTCTGTGCGGAAGTCAGATGCGTTTTACGGGGCTGAAGCGGGGTTACCGACTGGCAGAGCTGGTCCT
GATGCATGAGCGACTTGGCACGACAGCAGGTGTGCGGCTGAGAGCCGCAGAGGGGAAGTTGCGTCCATTTTACCGGAAACGGAGCAAAAAACCGCCATTC
ATACCCTGTATCAATCAGTGTCATCCTGTTTAATAGTCGTTTCCGCTCATATGGTGCACAAGGGGTGTTGAAGAAACATCCGTTTTGTGGTGCTTTTTTA
GTCTTTTGGGGATTTAAATTCCTATCGAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1281 bp | 426 aa | 454 | 1734 | + | No |
Chemistry : Y2
ORF sequence :
MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVTESVTKIMACGTTLMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHI
VFTLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRK
YPELVIPDELAVEGNSKRDWNCFLDTHYRRGWNVNISRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGDEFMERFS
WHVADKGFRMVRYYGFLSPVKRRLLEEVVYVITETVRKTAMQIRWRGMYQRLLKVDPLKCILCGSQMRFTGLKRGYRLAELVLMHERLGTTAGVRLRAAE
GKLRPFYRKRSKKPPFIPCINQCHPV
VFTLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRK
YPELVIPDELAVEGNSKRDWNCFLDTHYRRGWNVNISRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGDEFMERFS
WHVADKGFRMVRYYGFLSPVKRRLLEEVVYVITETVRKTAMQIRWRGMYQRLLKVDPLKCILCGSQMRFTGLKRGYRLAELVLMHERLGTTAGVRLRAAE
GKLRPFYRKRSKKPPFIPCINQCHPV
Blast result :
Comments
IS91 produces both cointegrates (10-6) and simple insertions (10-4). IS91 always inserts with the same orientation into its specific targets. IS91 ORF is 36 % identical to that of IS801, and they are both related to the rolling-circle-type replication proteins of the pUB110 family of plasmids, with which they share 4 conserved motifs.
References
1] Zabala, J.C., de la Cruz, F., and Ortiz, J. (1982) J. Bacteriol. 151, 472-476.
2] Diaz-Aroca, E., de la Cruz, F., Zabala, J.C., and Ortiz, J.M. (1984) Mol. Gen. Genet. 193, 493-499.
3] Diaz-Aroca, E., Mendiola, M. V., Zabala, J.C., and de la Cruz, F. (1987) J. Bacteriol. 169, 442-443.
4] Mendiola, M.V., and de la Cruz, F. (1989) Mol. Microbiol. 3, 979-984.
5] Mendiola, M.V., and de la Cruz, F. (1992) Nucl. Acids Res. 20, 3521.
6] Mendiola, M.V., Jubete, Y., and de la Cruz, F. (1992) J. Bacteriol. 174, 1345-1351.
7] Mendiola, M.V., Bernales, I., and de la Cruz, F. (1994) Proc. Natl. Acad. Sci., USA, 91, 1922-1926
2] Diaz-Aroca, E., de la Cruz, F., Zabala, J.C., and Ortiz, J.M. (1984) Mol. Gen. Genet. 193, 493-499.
3] Diaz-Aroca, E., Mendiola, M. V., Zabala, J.C., and de la Cruz, F. (1987) J. Bacteriol. 169, 442-443.
4] Mendiola, M.V., and de la Cruz, F. (1989) Mol. Microbiol. 3, 979-984.
5] Mendiola, M.V., and de la Cruz, F. (1992) Nucl. Acids Res. 20, 3521.
6] Mendiola, M.V., Jubete, Y., and de la Cruz, F. (1992) J. Bacteriol. 174, 1345-1351.
7] Mendiola, M.V., Bernales, I., and de la Cruz, F. (1994) Proc. Natl. Acad. Sci., USA, 91, 1922-1926