IS870

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) IS870.1, IS870.2, IS870.3
Accession numberTranspositionOriginHost
Z18270 Y Agrobacterium vitis
Agrobacterium vitis plasmid pTiTm4
Agrobacterium vitis 2657 plasmid pTiAg57
Agrobacterium vitis plasmid pTiAB3
DNA section
IS Length : 1146 bp

Ends


IR Length : 10

IRL : GTGGTTTGATCGAGACAAAAGAGTCCGATTTTGGATTCCAATTTCTGCAT
IRR : GTGGTTTGATTCCAACACTTGGTGCCCACGCTTGACGGCTGCGATGATGT

Insertion site


Left flankDirect repeatRight flankDR Length
NNNGACCAACTAGGTAAAGNNN2
NNNGCTGACCTAGCTGGTANNN2
NNNGTCCGTCTAGCATCATNNN2

DNA sequence

GTGGTTTGATCGAGACAAAAGAGTCCGATTTTGGATTCCAATTTCTGCATGAATATGCGACGGTCCTGCATGCGCACAGGTGTCACATTTGAGGTTTCCG
CTGCCGACCGGGTTCGGCTGAACGCAATCGTTTCGGCAGGCAGCTCGCCGCAAAAGCATGTGTGGCGGGCGAAGATCATCTTGATGACCGACGAGGGGCT
GGGAACGGTCGCGATCATGGAGGCGACGGCTAAGTCGAAGACCTGCGTCTGGCGATGGCAGGAGCGCTTCATGACGGAAGGCGTAGACGGCCTTTTGCGC
GACAAGAGCCGGCCGCCCGGCACTGCGCCGCTTGAGGTTGACCTTGTCGACCGGGTTGTTGCTCTGACGCAGGAGCCGCCCACGCAGGAAGCCACTCACT
GGACGGTTCGCGCGATGGCAAAGGCTGTGGGGATTGCGGCGTCCTCAGTCGTCAAGATCTGGCATGAGCATGGTCTCGCACCCCATCGGTGGCGCAGCTT
TAAACTATCGAACGACAAGGCTTTCGCCGAGAAGCTTCACGATGTGGTCGGTCTCTACGTCTCGCCGCCTGCCCATGCGATTGTGCTTTCCGTCGATGAG
AAAAGTCAGATCCAGGCGCTGGATAGGACGCAGCCGGGTCTTCCCATGAAGAAAGGGCGCGCCGGCACAATGACCCATGATTACAAGCGCCACGGCACCA
CCACACTTTTCGCTGCCCTGAATGTTCTCGACGGCTCCGTCATCGGCCGCAACATGCAGCGCCACCGGCATCAGGAGTTTATCCGCTTTCTCAACACGAT
TGAGGCTCAGTTGCCGAAGAGTAAGGCGGTTCACGTCATCCTGGACAACTACGCCACACACAAACAGCCCAAAGTCCGGGCCTGGTTGGCACGGCATCCA
CGCTGGACCTTCCACTTCGTTCCGACATCATGTTCCTGGTTGAACGCCGTCGAGGGGTTCTTCGCAAAGCTCACCCGTCGACGGCTGAAGAACGGTGTCT
TTCATTCGGTCGTTGATCTTCAGGCAGCCATCAACCGCTTTATCAAAGAGCACAACGAAGAGCCGAAGCCGTTCATCTGGAAAGCAGACCCTGACGACAT
CATCGCAGCCGTCAAGCGTGGGCACCAAGTGTTGGAATCAAACCAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1101 bp366 aa491149+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNMRRSCMRTGVTFEVSAADRVRLNAIVSAGSSPQKHVWRAKIILMTDEGLGTVAIMEATAKSKTCVWRWQERFMTEGVDGLLRDKSRPPGTAPLEVDLV
DRVVALTQEPPTQEATHWTVRAMAKAVGIAASSVVKIWHEHGLAPHRWRSFKLSNDKAFAEKLHDVVGLYVSPPAHAIVLSVDEKSQIQALDRTQPGLPM
KKGRAGTMTHDYKRHGTTTLFAALNVLDGSVIGRNMQRHRHQEFIRFLNTIEAQLPKSKAVHVILDNYATHKQPKVRAWLARHPRWTFHFVPTSCSWLNA
VEGFFAKLTRRRLKNGVFHSVVDLQAAINRFIKEHNEEPKPFIWKADPDDIIAAVKRGHQVLESN

 

Blast result :
Comments
IS870 elements are associated with the iaaH-iaaM gene cluster located in the TB region of several Ti plasmids. The ORF stop codon is the TAG of the CTAG target site. Although CTAG seems to be the duplicated target, DRs of two (TA) or zero bp are also possible. A duplication of two bp has been retained, by analogy to the other members of the family.
References
1] Fournier, P., Paulus, F., and Otten, L. (1993) J. Bacteriol. 175, 3151-3160.