IS701
- Family IS701
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X60383 | ND | Calothrix sp. | Pseudanabaena 7955 Calothrix sp. PCC7601 Calothrix PCC7504 Cylindrospermum 73101 Nostoc 6720 Nostoc 73102 Microcystis 7806 Microcystis 7820 Pseudanabaena 6903 |
DNA section
IS Length : 1389 bp
Ends
IR Length : 22/25
IRL : CCCTGTTTTGTCACTCTGGCAGTAGTATAATAAGGTAAAAATGCTAGAAC
IRR : CCCTCTTTTGTCACTCTAGGCAGAGGAAAAGTAGAAATCAGGGTGAATCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | TTAG | NNNNNNNNNN | 4 |
DNA sequence
CCCTGTTTTGTCACTCTGGCAGTAGTATAATAAGGTAAAAATGCTAGAACCAAGACACAGAGCATGGTACAGCCCCGTCCAGCCGCACCAACAGTCAAAT
TTGTGGACGAATATTGCCAGTGGTATAAAAGTCTGTTTCCAGATGTTAGGAGTTTCGAGGCTTTTAAATATCTCCATGTAGGCTGCATTTCTGATCTAAA
ACGTAAAACATTGCCAGAAATAGCAAAAATCGTAGGATTAGATAACCAGCAAGGGTTGCATCATTTTCTAACTACATCACCTTGGGATATAGAAAAGTTA
AGAACCTTAAGGTTAGAGTTAATTTTACAAGTGCTAAAAGGTAGACCAATCATTTTAATTATTGATGAGACAGGGGATAAAAAGAAAGGGAGCAAGACAG
ATTATGTGAAACGGCAGTATATAGGAAATTTGGGAAAAACAGATAATGGAATTGTGGCAGTGACAGTATATGGTGTTTTCTGTGGGATGACATTTCCATT
ACTGTTTGAAGTGTATAAACCCAGGGAAAGATTACAGGCAGGAGATAAGTACCGCACTAAACCAGAAATAGCAGCAATACTGATAAAAAAGCTACAATCA
ATGGGTTTTAAATTCAACTTAGTACTTGCAGATAGCTTATATGGAGAGAGTGGTAAGAATTTCATATCTGTATTAGATGAACTAAACTTGAACTATATAG
TAGCGATTCGGTCAAATCATTATGTAGAAATACTTCCACGACAACATATTCAATATTTAAAGTGGCAGAAGTTTCAAAGGGTATTCTCTGACTTGAGTCG
GGAAAATCGATTTATTAGAGAAATTATTCCGGGAAAACGTGGAGAACTTAGATATTGGCAAATTACTACAGATCCAGAAAATTTGCCTGATAACACTACT
TGGTATGTGATGAGTAAATATCCAGACATTACGCCAAGAGAAGTTGGAAATTTTTACGGTTTAAGAACTTGGGTCGAGTACGGGTTAAAACAAAGTAAGA
ATGAATTAGGTTGGTCAGATTTTCGCCTGACTCACTACCCAGATATTGAGGCGATGGTGGGAAATTATTTGCAGTGCTTATTTAATGGAGTCTGCATTCG
GAGCAACTGTTTCAGTCTCCATCACAACGAGAGTCAAAATTTGTTTCACATCCTTGGTGGGATAATGGAAATGGCTGGAAGAACATTCTTAACAATCTTC
GTTTGATTATTCAACCTTTTACTTTATTTAATCTGATATATCCCTGGTTAACGGTTTTTCCTATTCCTCAAATTAGCTTTGGGTTTTTTTAAACTTCAAT
CTATTATTTATAGCCTCACCAGTTCAATTTTTATATCCCTGATTCACCCTGATTTCTACTTTTCCTCTGCCTAGAGTGACAAAAGAGGG
TTGTGGACGAATATTGCCAGTGGTATAAAAGTCTGTTTCCAGATGTTAGGAGTTTCGAGGCTTTTAAATATCTCCATGTAGGCTGCATTTCTGATCTAAA
ACGTAAAACATTGCCAGAAATAGCAAAAATCGTAGGATTAGATAACCAGCAAGGGTTGCATCATTTTCTAACTACATCACCTTGGGATATAGAAAAGTTA
AGAACCTTAAGGTTAGAGTTAATTTTACAAGTGCTAAAAGGTAGACCAATCATTTTAATTATTGATGAGACAGGGGATAAAAAGAAAGGGAGCAAGACAG
ATTATGTGAAACGGCAGTATATAGGAAATTTGGGAAAAACAGATAATGGAATTGTGGCAGTGACAGTATATGGTGTTTTCTGTGGGATGACATTTCCATT
ACTGTTTGAAGTGTATAAACCCAGGGAAAGATTACAGGCAGGAGATAAGTACCGCACTAAACCAGAAATAGCAGCAATACTGATAAAAAAGCTACAATCA
ATGGGTTTTAAATTCAACTTAGTACTTGCAGATAGCTTATATGGAGAGAGTGGTAAGAATTTCATATCTGTATTAGATGAACTAAACTTGAACTATATAG
TAGCGATTCGGTCAAATCATTATGTAGAAATACTTCCACGACAACATATTCAATATTTAAAGTGGCAGAAGTTTCAAAGGGTATTCTCTGACTTGAGTCG
GGAAAATCGATTTATTAGAGAAATTATTCCGGGAAAACGTGGAGAACTTAGATATTGGCAAATTACTACAGATCCAGAAAATTTGCCTGATAACACTACT
TGGTATGTGATGAGTAAATATCCAGACATTACGCCAAGAGAAGTTGGAAATTTTTACGGTTTAAGAACTTGGGTCGAGTACGGGTTAAAACAAAGTAAGA
ATGAATTAGGTTGGTCAGATTTTCGCCTGACTCACTACCCAGATATTGAGGCGATGGTGGGAAATTATTTGCAGTGCTTATTTAATGGAGTCTGCATTCG
GAGCAACTGTTTCAGTCTCCATCACAACGAGAGTCAAAATTTGTTTCACATCCTTGGTGGGATAATGGAAATGGCTGGAAGAACATTCTTAACAATCTTC
GTTTGATTATTCAACCTTTTACTTTATTTAATCTGATATATCCCTGGTTAACGGTTTTTCCTATTCCTCAAATTAGCTTTGGGTTTTTTTAAACTTCAAT
CTATTATTTATAGCCTCACCAGTTCAATTTTTATATCCCTGATTCACCCTGATTTCTACTTTTCCTCTGCCTAGAGTGACAAAAGAGGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1143 bp | 380 aa | 64 | 1206 | + | No |
Chemistry : DDE
ORF sequence :
MVQPRPAAPTVKFVDEYCQWYKSLFPDVRSFEAFKYLHVGCISDLKRKTLPEIAKIVGLDNQQGLHHFLTTSPWDIEKLRTLRLELILQVLKGRPIILII
DETGDKKKGSKTDYVKRQYIGNLGKTDNGIVAVTVYGVFCGMTFPLLFEVYKPRERLQAGDKYRTKPEIAAILIKKLQSMGFKFNLVLADSLYGESGKNF
ISVLDELNLNYIVAIRSNHYVEILPRQHIQYLKWQKFQRVFSDLSRENRFIREIIPGKRGELRYWQITTDPENLPDNTTWYVMSKYPDITPREVGNFYGL
RTWVEYGLKQSKNELGWSDFRLTHYPDIEAMVGNYLQCLFNGVCIRSNCFSLHHNESQNLFHILGGIMEMAGRTFLTIFV
DETGDKKKGSKTDYVKRQYIGNLGKTDNGIVAVTVYGVFCGMTFPLLFEVYKPRERLQAGDKYRTKPEIAAILIKKLQSMGFKFNLVLADSLYGESGKNF
ISVLDELNLNYIVAIRSNHYVEILPRQHIQYLKWQKFQRVFSDLSRENRFIREIIPGKRGELRYWQITTDPENLPDNTTWYVMSKYPDITPREVGNFYGL
RTWVEYGLKQSKNELGWSDFRLTHYPDIEAMVGNYLQCLFNGVCIRSNCFSLHHNESQNLFHILGGIMEMAGRTFLTIFV
Blast result :
Comments
IS701 was originally isolated as a spontaneous insertion into the cpcF gene (encoding the beta subunit of the phycocyanin, a phycobiliprotein involved in light harvesting), giving rise to a pigmentation mutant. Hybridization experiments showed that at least 15 copies of IS701 are present in the Calothrix sp. PCC7601 genome (Mazel et al., 1991). IS701 putative transposase is 28 % identical (337-aa overlap) to that of IS1452 from Acetobacter pasteurianus NCI1452.
References
1] Mazel, D., Bernard, C., Schwartz, R., Castets, A.M., Houmard, J., and Tandeau de Marsac, N. (1991) Mol. Microbiol. 5, 2165-2170.