ISfinder Mirror Site

IS651

Family ISL3
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_002570	ND	Bacillus halodurans	Bacillus halodurans C-125

DNA section

IS Length : 1384 bp

Ends

IR Length : 18/23

IRL : GGCTCTACAATAAATGTTGGGGTTTTTGCACAAAAGAGGGCAAAAAAAAT
IRR : GGTTCTTAGTCAAATGTTGGGGTGGCATAGGAGGGCGAGACCCTCTTATC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTTCTGATTT	GCAATTTA	AGAAATCGAT	8
GTCTTTGCTT	GATAAGTA	TAAATGATCG	8
AATTAATGCC	TCTCGTTT	AAGCTCTGCC	8
AGGCTTGTGA	TTTTTTTA	TTGAGAGGAA	8
GCAAACTGCT	AATAAAAA	AGAACATACT	8
GTTTTTGTTT	TGT	TTTTTCACTT	3
GCCTTAAGCT	TTTTTTT	GAATGATCGG	7
GTGAGGTTCT	TTTTTTAA	TCATAAAATA	8
TTTTTCTACG	AAAAAATC	GTGCAACTAG	8
GTGAAGCCGC	CCATTTTC	GTTGCAGCTG	8
GGTGCCTTTT	TTATTTAT	CGAGGTTGTA	8
AGTAAGCAGG	AGTTTTTA	ACATCCCCTT	8
GTTTTTTGTT	TAAATTTC	AAGGAGGTGA	8
TCCCGATTTT	ATGTTTTA	AGGTGCTTTT	8
GGCTGCGTCT	TTCTTTTG	TCATTGGAAG	8
TACTGCTCTT	GTTTTTCC	CGCAGGTGTC	8
GCTAGTCTTT	TTGATTTT	TGCCCCTGTT	8
ACACTTGGCG	AAAAAACA	AGTTCAGATT	8

DNA sequence

GGCTCTACAATAAATGTTGGGGTTTTTGCACAAAAGAGGGCAAAAAAAATGCACGCAGGCTCCTTTATCCATTATCCTTGAATTGTCCAGAAACAAAGAA
AGGATAGGAGCTGCGTGTACGATGAATTTTACCATGGATTTGCCCGGATTAAAAGGTGTGAAGGTCACAAAGATGGAGGAACGAGAGGGCTTTATCGCGC
TCCATGTAGAAATGCCGCGAACGCCTCATCGTTGCCCATCCTGCGGGGAGAGAACCGAGAGAACCCATGATTACCGCATCCAAAAAGTTCAACATCTCAA
GCTCTTTGAACGGTGGGTGTATCTATTCTATCGAAAGCGCCGGTACGCGTGCAGATGCGGGAAGCGGTTTGCGGAGAAAGCCTGTTTTGTAGAGCGATAT
CAGCGTCAGACAGTAGAATGGAATCAAGCGTTTCGCTTACGGCTCATTCAAGGGAAGAACTTTACGGACACAGCGAATCAATTCCAAACGTCCGTGACAA
CTGCCATGCGCCGGTTTGATCAATTGGCGGCCCCCCTCGTGAAGAAGGTGGATCACCTTCCTCCTCGGATTGCGATTGATGATTACAAAGGGGATACGAA
TGCGGGGAAATATCAAGTGATCATTGCGGATGCAGATACACGGAAGCCGTTGGACATCCTACCGGATCGTAAAGCCGATACGCTTAAACGATATCTTCAA
GAGAAGGGTCATCATGTGAAAACAGTCGTGATGGATCTAAGTTATACCTTCAAGTCGGCTGTTCAAAAGGCCTTAGGCCACCCGCTCATCATTGGGGATC
CGTTCCATTTCTGTCGTTACATCTACTGGGCTCTGGAAGGGGTTCGCCGCCGAGTGCAAAAAGCGTTCCATCCGTATCATCGAAAACGATGTAAACGGAT
CAAACATCTGTTTCAGAAAGAGAGAGAAAGACTGGATGATAAGCAAAAGAAGAAGGTGGAGCGGTACTTAGAGCTATCCCCTGATTTAAGGGAAGCGTAT
GAGCTAAAAGAACATTTTCGTCGGTGGTATCAAGAAGCGAAGATGCAAGGAAGGGAAGGACGAATGAGCGAGGTGAAACAAGGGCTCTACGCCTTTTACC
GAAGAGTGGAGGCGAGCCGGTTAAAGGAGTTCCACCGAGCCATTCAGACGCTGAAGAACTGGCAGACCGAGATCTTGAATAGCTTCGCGTTTGGCTTGAA
CAATGGTTTTATTGAAGGGCTGAATAATCAAACGAAGGTGATCAAGAGAAACGCCTTTGGTTTTCGGCGTTATGATCGCTTGCGAAACCGCATTCTATTA
CATCATCAATTCAAACATCAGACATTCGGGGTAGGATAAGAGGGTCTCGCCCTCCTATGCCACCCCAACATTTGACTAAGAACC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1218 bp	405 aa	122	1339	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MNFTMDLPGLKGVKVTKMEEREGFIALHVEMPRTPHRCPSCGERTERTHDYRIQKVQHLKLFERWVYLFYRKRRYACRCGKRFAEKACFVERYQRQTVEW
NQAFRLRLIQGKNFTDTANQFQTSVTTAMRRFDQLAAPLVKKVDHLPPRIAIDDYKGDTNAGKYQVIIADADTRKPLDILPDRKADTLKRYLQEKGHHVK
TVVMDLSYTFKSAVQKALGHPLIIGDPFHFCRYIYWALEGVRRRVQKAFHPYHRKRCKRIKHLFQKERERLDDKQKKKVERYLELSPDLREAYELKEHFR
RWYQEAKMQGREGRMSEVKQGLYAFYRRVEASRLKEFHRAIQTLKNWQTEILNSFAFGLNNGFIEGLNNQTKVIKRNAFGFRRYDRLRNRILLHHQFKHQ
TFGVG

Blast result :

Comments

There is 22 copies of IS651 on Bacillus halodurans chromosome.

References

1] Takami H, Han CG, Takaki Y, Ohtsubo E. (2001) J. Bacteriol. 183(14), 4345-56.