ISfinder Mirror Site

IS640

Family IS21
Group

MGE type ISRelated element(s) :
Isoform IS21Synonym(s)

Accession number	Transposition	Origin	Host
HE616528	ND	Shigella sonnei	Shigella sonnei Shigella sonnei 53G

DNA section

IS Length : 2130 bp

Ends

IR Length : 30/38

IRL : TGTCAGCGCCAGTGATATAAGACGGTAATTCGCCATTTGGATTGTCCGCT
IRR : TGTCAACGCCACGATGTTTGACCGTTATTTGCCATTTTCATGCCCGCTAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCTTTCTCGA	CCGC	TTCGATCTCT	4

DNA sequence

TGTCAGCGCCAGTGATATAAGACGGTAATTCGCCATTTGGATTGTCCGCTCCACCCAACATGTTGTTTCCTTAAGGTTCTCACACCAGAAAGGACATCAA
CATGCTGAGCAGAGAGGACTTTTACATGATAAAGCAAATGCGCCAGCAGGGCGCGTACATCGTCGATATTGCGACTCAGATTGGTTGCTCTGAACGGACG
GTCAGACGCTACCTCAAATACCCTGAACCGCCAGCCAGAAAGACCCGCCACAAAATGGTTAAGCTGAAACCGTTTATGGATTACATCGACATGCGCCTGG
CAGAGAATGTCTGGAATAGCGAGGTTATCTTCGCGGAGATTAAGGCAATGGGTTATACGGGCGGACGTTCCATGCTGCGTTACTACATCCAGCCCAAACG
TAAAATGCGTCCGTCAAAAAGAACAGTTCGCTTCGAAACTCAGCCTGGATACCAGCTCCAGCATGACTGGGGCGAAGTTGAGGTGGAGGTTGCCGGGCAA
CGGTGCAAAGTTAACTTTGCGGTTAATACGCTGGGGTTCTCCCGCAGCTTCCATGTCTTCGCCGCACCAAAACAGGATGCTGAGCATACCTACGAATCAC
TGGTTCGCGCCTTCCGCTACTTCGGTGGTTGTGTGAAAACGGTGCTGGTTGATAACCAGAAGGCTGCGGTGCTGAAGAATAACAACGGGAAAGTCGTGTT
CAACTCCGGATTCCTGTTGCTGGCTGACCACTATAACTTCCTGCCACGGGCATGCCGTCCACGCAGGGCCAGAACAAAAGGTAAGGTTGAGCGGATGGTG
AAATACCTCAAGGAGAACTTCTTCGTCCGGTACCGCAGGTTCGACAGCTTCACTCATGTCAATCAACAACTGGAGCAATGGATAGCCGATGTGGCTGACA
AACGGGAGCTTCGCCAGTTCAAAGAAACGCCGGAACAGCGCTTCGCGCTGGAGCAGGAACATCTGCAGCCGTTACCGGATACAGACTTCGATACCAGTTA
CTTCGATATCCGCCATGTGTCCTGGGACAGCTATATCGAGGTTGGTGGTAATCGTTACAGCGTTCCCGAAGCGCTGTGTGGTCAGCCGGTATCGATACGA
ATATCGCTGGATGACGAGTTGCGGATCTACAGTAATGAGAAACTGGTGGCCTCACATCGCCTCTGTTCAGCATCGTCTGGCTGGCAGACAGTGCCGGAGC
ATCACGCCCCGCTCTGGCAGCAGGTCAGTCAGGTGGAACATCGACCACTGAGTGCCTATGAGGAGCTGTTGTGATGCATGAACTGGAAGTCCTGCTGAGT
CGCCTGAAAATGGAGCATCTGAGTTATCACGTTGAAAGCCTGCTGGAACAGGCAGCTAAAAAAGAGCTGAACTACCGGGAGTTCCTGTGCATGGCGCTAC
AGCAGGAATGGAACGGCAGGCATCAGCGCGGTATGGAGTCCAGGCTGAAGCAGGCTCGTCTGCCGTGGGTCAAAACGCTGGAGCAGTTCGACTTTACCTT
CCAGCCGGGCATCGACCGTAAGGTTGTCCGGGAACTGGCTGGTCTGGCGTTCGTGGAGCGCAGCGAAAACGTGATCCTGCTGGGCCCTCCTGGTGTCGGA
AAAACTCATCTGGCCATAGCTCTTGGCGTGAAAGCGGTGGATGCGGGACATCGGGTACTGTTTATGCCACTGGACAGACTGATCGCGACACTGATGAAAG
CGAAACAGGAAAACCGGCTGGAGCGTCAGCTACAGCAACTGAGTTATGCCCGGGTGTTGATCCTGGATGAAATAGGCTATCTGCCGATGAACAGAGAGGA
AGCCAGTCTGTTCTTCCGGCTACTGAACCGTCGATATGAAAAAGCGAGCATCATACTGACGTCAAACAAAGGGTTCGCAGACTGGGGAGAAATGTTCGGA
GATCACGTACTGGCAACAGCGATCCTGGATCGGCTGCTACATCACTCAACCACGCTGAATATCAAAGGAGAGAGTTACCGGTTAAAAGAGAAACGTAAAG
CTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGGAAAGCGGACAGCATCAGTAACGAAAGTATTTAGCGGGCATGAAAATGGC
AAATAACGGTCAAACATCGTGGCGTTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1173 bp	390 aa	102	1274	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLSREDFYMIKQMRHEGAYIVDIATQIGCSERTVRRYLKYPEPPARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKR
KMRPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKHDAEHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVF
NSGFLLLADHYNFLPRACRPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRSRWSRNICSVTGYRLRYQLL
RYPPCVLGQLYRGWW

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
798 bp	265 aa	1274	2071	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

Blast result :

Comments

The first founded IS640 sequence (accession number :X05956) is 98.2 % identical to the first 1085 bp of IS21.
June 19 2012 : The file was modified with the most closely related sequence founded at NCBI (99% DNA identity). The sequence is in fact an isoform of IS21.
IS640 is 99% aa similar to IS21.

References

1] Matsutani, S., Ohtsubo, H., Maeda, Y., and Ohtsubo, E. (1987) J. Mol. Biol. 196, 445-455.