IS630
- Family IS630
- Group
Isoform Synonym(s) Tn4730, Tn4731, Tn4733
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X05955 | Y | Shigella sonnei | Shigella sonnei Shigella flexneri Escherichia coli JE5519 |
DNA section
IS Length : 1153 bp
Ends
IR Length : 21/29
IRL : AATAGCTGCGCGGAATAGTAGATCACTTTGAGGGAACTTAGCCCGGATTG
IRR : AATAGCTGCGCTTAATACCGCTACACTTTTGCCAGCCCATGTTTGCCTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
AATAGCTGCGCGGAATAGTAGATCACTTTGAGGGAACTTAGCCCGGATTGTGCGATCTGATCAATCGCCAAATCAAAACAAATCACCAACCGGACTGAGC
AATGCCGATCATAGCACCAATTTCCCGTGACGAACGACGCCTGATGCAGAAAGCCATCCATAAAACACACGATAAAAATTATGCCCGCAGACTGACTGCC
ATGCTGATGCTGCACCGGGGCGACCGTGTCAGCGACGTTGCCAGAACGCTCTGCTGCGCCCGTTCCTCTGTTGGACGCTGGATTAACTGGTTCACGCAGT
CGGGTGTTGAGGGACTGAAATCATTACCTGCCGGGCGTGCCCGTCGCTGGCCGTTTGAGCATATCTGCACACTGTTACGTGAGCTGGTAAAACATTCTCC
CGGCGACTTTGGCTACCAGCGTTCACGCTGGAGTACAGAACTGCTGGCAATAAAAATCAATGAGATAACCGGTTGCCAGTTAAATGCCGGAACCGTTCGC
CGCTGGTTGCCGTCTGCGGGGATTGTGTGGCGAAGGGCTGCGCCAACTCTGCGTATCCGTGACCCGCATAAAGATGAAAAGATGGCAGCAATCCATAAAG
CACTGGACGAATGCAGCGCAGAGCATCCGGTCTTTTATGAAGATGAAGTGGATATCCATCTTAATCCCAAAATCGGTGCGGACTGGCAACTGCGCGGACA
GCAAAAACGGGTGGTCACGCCGGGACAGAATGAAAAATATTATCTGGCCGGAGCGCTGCACAGCGGGACAGGTAAAGTCAGCTGTGTGGGCGGCAACAGC
AAAAGTTCGGCGCTGTTCATCAGCCTGCTGAAGCGGCTTAAAGCGACATACCGTCGGGCGAAAACCATCACGCTGATCGTGGACAACTACATTATCCACA
AAAGCCGGGAAACACAGAGCTGGCTGAAGGAGAACCCGAAGTTCAGGGTCATTTATCAGCCGGTTTACTCGCCATGGGTGAATCATGTTGAACGGCTATG
GCAGGCACTTCACGACACAATAACGCGTAATCATCAGTGCAGCTCAATGTGGCAACTGTTGAAAAAAGTTCGCCATTTTATGGAAACCGTCAGCCCATTC
CCCGGAGGCAAACATGGGCTGGCAAAAGTGTAGCGGTATTAAGCGCAGCTATT
AATGCCGATCATAGCACCAATTTCCCGTGACGAACGACGCCTGATGCAGAAAGCCATCCATAAAACACACGATAAAAATTATGCCCGCAGACTGACTGCC
ATGCTGATGCTGCACCGGGGCGACCGTGTCAGCGACGTTGCCAGAACGCTCTGCTGCGCCCGTTCCTCTGTTGGACGCTGGATTAACTGGTTCACGCAGT
CGGGTGTTGAGGGACTGAAATCATTACCTGCCGGGCGTGCCCGTCGCTGGCCGTTTGAGCATATCTGCACACTGTTACGTGAGCTGGTAAAACATTCTCC
CGGCGACTTTGGCTACCAGCGTTCACGCTGGAGTACAGAACTGCTGGCAATAAAAATCAATGAGATAACCGGTTGCCAGTTAAATGCCGGAACCGTTCGC
CGCTGGTTGCCGTCTGCGGGGATTGTGTGGCGAAGGGCTGCGCCAACTCTGCGTATCCGTGACCCGCATAAAGATGAAAAGATGGCAGCAATCCATAAAG
CACTGGACGAATGCAGCGCAGAGCATCCGGTCTTTTATGAAGATGAAGTGGATATCCATCTTAATCCCAAAATCGGTGCGGACTGGCAACTGCGCGGACA
GCAAAAACGGGTGGTCACGCCGGGACAGAATGAAAAATATTATCTGGCCGGAGCGCTGCACAGCGGGACAGGTAAAGTCAGCTGTGTGGGCGGCAACAGC
AAAAGTTCGGCGCTGTTCATCAGCCTGCTGAAGCGGCTTAAAGCGACATACCGTCGGGCGAAAACCATCACGCTGATCGTGGACAACTACATTATCCACA
AAAGCCGGGAAACACAGAGCTGGCTGAAGGAGAACCCGAAGTTCAGGGTCATTTATCAGCCGGTTTACTCGCCATGGGTGAATCATGTTGAACGGCTATG
GCAGGCACTTCACGACACAATAACGCGTAATCATCAGTGCAGCTCAATGTGGCAACTGTTGAAAAAAGTTCGCCATTTTATGGAAACCGTCAGCCCATTC
CCCGGAGGCAAACATGGGCTGGCAAAAGTGTAGCGGTATTAAGCGCAGCTATT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1032 bp | 343 aa | 102 | 1133 | + | No |
Chemistry : DDE
ORF sequence :
MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVSDVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSP
GDFGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQ
QKRVVTPGQNEKYYLAGALHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRVIYQPVYSPWVNHVERLW
QALHDTITRNHQCSSMWQLLKKVRHFMETVSPFPGGKHGLAKV
GDFGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQ
QKRVVTPGQNEKYYLAGALHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRVIYQPVYSPWVNHVERLW
QALHDTITRNHQCSSMWQLLKKVRHFMETVSPFPGGKHGLAKV
Blast result :
Comments
The IS630 family includes the Caenorhabditis elegans Tc1, Drosophila mariner, Euplotes crassus Tec1 and Oxytricha fallax TBE1 mobile elements (Doak et al., 1994).
Additionnal Accession number : AF294823 . Shigella sonnei , 99% aa similar to the sequence of the database (mutation at position 293 V -> M ).
Additionnal Accession number : AF294823 . Shigella sonnei , 99% aa similar to the sequence of the database (mutation at position 293 V -> M ).
References
1] Matsutani, S., Ohtsubo, H., Maeda, Y., and Ohtsubo, E. (1987) J. Mol. Biol. 196, 445-455.
2] Tenzen, T., Matsutani, S., and Ohtsubo, E. (1990) J. Bacteriol. 172, 3830-3836.
3] Tenzen, T., and Ohtsubo, E. (1991) J. Bacteriol. 173, 6207-6212.
4] Fournier, P., Paulus, F., and Otten, L. (1993) J. Bacteriol. 175, 3151-3160.
5] Vos, J.C., van Luenen, H.G.A.M., and Plasterk, R.H.A. (1993) Genes & Dev. 7, 1244-1253.
6] Doak, T.G., Doerder, F.P., Jahn, C.L., and Herrick, G. (1994) Proc. Natl. Acad. Sci., USA 91, 942-946.
7] Capy, P., Langin, T., Bigot, Y., Brunet, F., Daboussi, M.J., Periquet, G., David, J.R., and Hartl, D.L. (1994) Genetica 93, 161-170.
8] Xu,D.Q., Cisar,J.O., Ambulos,N. Jr., Burr,D. and Kopecko,D.J. (2000) Direct submission GenBank.
2] Tenzen, T., Matsutani, S., and Ohtsubo, E. (1990) J. Bacteriol. 172, 3830-3836.
3] Tenzen, T., and Ohtsubo, E. (1991) J. Bacteriol. 173, 6207-6212.
4] Fournier, P., Paulus, F., and Otten, L. (1993) J. Bacteriol. 175, 3151-3160.
5] Vos, J.C., van Luenen, H.G.A.M., and Plasterk, R.H.A. (1993) Genes & Dev. 7, 1244-1253.
6] Doak, T.G., Doerder, F.P., Jahn, C.L., and Herrick, G. (1994) Proc. Natl. Acad. Sci., USA 91, 942-946.
7] Capy, P., Langin, T., Bigot, Y., Brunet, F., Daboussi, M.J., Periquet, G., David, J.R., and Hartl, D.L. (1994) Genetica 93, 161-170.
8] Xu,D.Q., Cisar,J.O., Ambulos,N. Jr., Burr,D. and Kopecko,D.J. (2000) Direct submission GenBank.