IS629
- Family IS3
- Group IS51
Isoform IS3411Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X51586 | ND | Shigella sonnei | Shigella flexneri 2a Shigella sonnei Bacteriophage 21 Escherichia coli EC38 |
DNA section
IS Length : 1310 bp
Ends
IR Length : 23/25
IRL : TGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAA
IRR : TGAACCGCCCCGGGTTTCCTGGAGAGTGTTTTATCTGTGAACTCAGGCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAG
TCCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGT
CCGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAGCGTGAAAATCGTGAA
CTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGCGGAGTTTGACCGCCTCTGGAAAAAATGATGCCACTGCTGGATAAGCT
GCGTGAGCAGTACGGGGTCGGACCGCTATGCAGCGAACTGCATATTGCCCCGTCAACGTATTACCACTGTCAGCAACAGCGACATCATCCGGATAAACGC
AGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGT
TGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAC
CATCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGGTGGCTGATTTTACTTACGTCAGC
ACATGGCAGGGCTTCGTCTATGTGGCGTTCATCATTGATGTGTTTGCCGGATACATCGTGGGGTGGCGGGTCTCATCGTCCATGGAAACGACATTCGTGC
TGGATGCACTGGAGCAGGCGTTATGGGCCCGTCGACCGTCCGGCACGGTCCATCACAGTGATAAAGGTTCTCAGTATGTATCGCTGGCCTACACACAGCG
GCTTAAGGAAGCCGGATTACTGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCGATGGCGGAGAGCATCAATGGTCTTTACAAAGCGAAGGTA
ATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTCACGTGGGTGGACTGGTATAACAATCGACGATTGCTGGAAAGGCTGGGCC
ATACTCCTCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGAGTTCACAGATAAAACACTCTCCAGGAAACCCG
GGGCGGTTCA
TCCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGT
CCGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAGCGTGAAAATCGTGAA
CTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGCGGAGTTTGACCGCCTCTGGAAAAAATGATGCCACTGCTGGATAAGCT
GCGTGAGCAGTACGGGGTCGGACCGCTATGCAGCGAACTGCATATTGCCCCGTCAACGTATTACCACTGTCAGCAACAGCGACATCATCCGGATAAACGC
AGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGT
TGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAC
CATCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGGTGGCTGATTTTACTTACGTCAGC
ACATGGCAGGGCTTCGTCTATGTGGCGTTCATCATTGATGTGTTTGCCGGATACATCGTGGGGTGGCGGGTCTCATCGTCCATGGAAACGACATTCGTGC
TGGATGCACTGGAGCAGGCGTTATGGGCCCGTCGACCGTCCGGCACGGTCCATCACAGTGATAAAGGTTCTCAGTATGTATCGCTGGCCTACACACAGCG
GCTTAAGGAAGCCGGATTACTGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCGATGGCGGAGAGCATCAATGGTCTTTACAAAGCGAAGGTA
ATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTCACGTGGGTGGACTGGTATAACAATCGACGATTGCTGGAAAGGCTGGGCC
ATACTCCTCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGAGTTCACAGATAAAACACTCTCCAGGAAACCCG
GGGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 55 | 381 | + | No |
Description : First part of the transposase
ORF sequence :
MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTPETLRVRVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKA
EFDRLWKK
EFDRLWKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 363 | 1268 | + | No |
Description : Second part of the transposase
ORF sequence :
PPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMG
LAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSD
KGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAKVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLA
A
LAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSD
KGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAKVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLA
A
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1214 bp | 404 aa | 55 | 1268 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTPETLRVRVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKA
EFDRLWKKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMA
VMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVH
HSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAKVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGND
DLAA
EFDRLWKKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMA
VMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVH
HSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAKVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGND
DLAA
Blast result :
Comments
Nearly identical to IS3411. IS629 ORF1 and ORF2 are 97.2 and 98.3 % identical to those of IS3411.
IS629 is also present in Bacteriophage 21 (Accession number : AF017626 ), this sequence is 99% DNA similar to those of the database.
Additionnal accession number : AF294823 . This sequence is 99% aa similar to the sequence of the database (mutation at position 54 : H -> Y ).
Additionnal accession number : AF326777 (Shigella flexneri 2a ; 2001) . This sequence is 99% DNA and 98% (ORF1 and ORF2) aa similar to the sequence of the database. For the ORF1, there are mutations at positions 24 (G -> S) and 50 (R -> W) and for the ORF 2, there is a mutation at position 195 (A -> V).
Bacteriophage 21 (specific_host : Escherichia coli strain EC38)
IS629 is also present in Bacteriophage 21 (Accession number : AF017626 ), this sequence is 99% DNA similar to those of the database.
Additionnal accession number : AF294823 . This sequence is 99% aa similar to the sequence of the database (mutation at position 54 : H -> Y ).
Additionnal accession number : AF326777 (Shigella flexneri 2a ; 2001) . This sequence is 99% DNA and 98% (ORF1 and ORF2) aa similar to the sequence of the database. For the ORF1, there are mutations at positions 24 (G -> S) and 50 (R -> W) and for the ORF 2, there is a mutation at position 195 (A -> V).
Bacteriophage 21 (specific_host : Escherichia coli strain EC38)
References
1] Matsutani, S., Ohtsubo, H., Maeda, Y., and Ohtsubo, E. (1987) J. Mol. Biol. 196, 445-455.
2] Matsutani, S., and Ohtsubo, E. (1990) Nucl. Acids Res. 18, 1899.
3] Wang,F.S., Whittam,T.S. and Selander,R.K.(1997) J. Bacteriol. 179 (21), 6551-6559
4] Luck,S.N., Turner,S.A., Rajakumar,K., Sakellaris,H. and Adler,B. (2001) Infect. Immun. 69 (10), 6012-6021
5] Xu,D.Q., Cisar,J.O., Ambulos,N. Jr., Burr,D. and Kopecko,D.J. (2000) Direct submission GenBank.
2] Matsutani, S., and Ohtsubo, E. (1990) Nucl. Acids Res. 18, 1899.
3] Wang,F.S., Whittam,T.S. and Selander,R.K.(1997) J. Bacteriol. 179 (21), 6551-6559
4] Luck,S.N., Turner,S.A., Rajakumar,K., Sakellaris,H. and Adler,B. (2001) Infect. Immun. 69 (10), 6012-6021
5] Xu,D.Q., Cisar,J.O., Ambulos,N. Jr., Burr,D. and Kopecko,D.J. (2000) Direct submission GenBank.