IS6100

  • Family IS6
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) IS6100L, IS6100R, Tn610
Accession numberTranspositionOriginHost
X53635 Y Mycobacterium fortuitum
Pseudomonas aeruginosa
Xanthomonas campestris pv. vesicatoria BV5-4a
Mycobacterium smegmatis mc2155
Mycobacterium fortuitum FC1
Arthrobacter sp. KI72 plasmid pOAD2
Pseudomonas sp. NK87 plasmid pNAD2
DNA section
IS Length : 880 bp

Ends


IR Length : 14

IRL : GGCTCTGTTGCAAAAATCGTGAAGCTTGAGCATGCTTGGCGGAGATTGGA
IRR : GGCTCTGTTGCAAAGATTGGCGGCAGTCAGAGGTAGGCTGTCGCTCTGCG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGCTCTGTTGCAAAAATCGTGAAGCTTGAGCATGCTTGGCGGAGATTGGACGGACGGAACGATGACGGATTTCAAGTGGCGCCATTTCCAGGGTGATGTG
ATCCTGTGGGCGGTGCGCTGGTATTGTCGCTATCCGATCAGCTATCGCGACCTTGAGGAAATGCTGGCGGAACGCGGCATTTCGGTCGACCATACGACGA
TCTATCGCTGGGTCCAGTGCTACGCCCCGGAGATGGAGAAGCGGCTGCGCTGGTTCTGGCGGCGTGGCTTTGATCCGAGCTGGCGCCTGGATGAAACCTA
CGTCAAGGTGCGGGGCAAGTGGACCTACCTGTACCGGGCAGTCGACAAGCGGGGCGACACGATCGATTTCTACCTGTCGCCGACCCGCAGCGCCAAGGCA
GCGAAGCGGTTCCTGGGCAAGGCCCTGCGAGGCCTGAAGCACTGGGAAAAGCCTGCCACGCTCAATACCGACAAAGCGCCGAGCTATGGTGCAGCGATCA
CCGAATTGAAGCGCGAAGGAAAGCTGGACCGGGAGACGGCCCACCGGCAGGTGAAGTATCTCAATAACGTGATCGAGGCCGATCACGGAAAGCTCAAGAT
ACTGATCAAGCCGGTGCGCGGTTTCAAATCGATCCCCACGGCCTATGCCACGATCAAGGGATTCGAAGTCATGCGAGCCCTGCGCAAAGGACAGGCTCGC
CCCTGGTGCCTGCAGCCCGGCATCAGGGGCGAGGTGCGCCTTGTGGAGAGAGCTTTTGGCATTGGGCCCTCGGCGCTGACGGAGGCCATGGGCATGCTCA
ACCACCATTTCGCAGCAGCCGCCTGATCGGCGCAGAGCGACAGCCTACCTCTGACTGCCGCCAATCTTTGCAACAGAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
765 bp254 aa62826+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLGGDWTDGTMTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHTTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYL
YRAVDKRGDTIDFYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQVKYLNNVIEADHGKLKILIKPVRGFKS
IPTAYATIKGFEVMRALRKGQARPWCLQPGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA

 

Blast result :
Comments
Tn611 (a Tn610 kanR derivative) has been derived into a thermosensitive transposition vector in plasmid pCG79, and an insertional mutant library has been obtained in Mycobacterium smegmatis (Guilhot et al., 1994). Similarly, the same element has also been incorporated into a conjugative, thermosensitive Escherichia coli - Mycobacterium smegmatis shuttle vector (Gavigan et al., 1995). Tn611 transposition generates cointegrate molecules in Mycobacterium smegmatis (Guilhot et al., 1994) and Streptomyces lividans (Smith and Dyson, 1995). Note that the DNA sequences of five RS sequences from the nylon oligomer degrading plasmid pOAD2 of Arthrobacter sp. are identical to that of IS6100, originally found in Mycobacterium smegmatis (Kato et al., 1994). In the Pseudomonas aeruginosa plasmid R1033, IS6100 is located downstream of Tn1696, at the 3'-end of the In4 integron (Hall et al., 1994). IS6100 is also present within the tnpR gene of the strR transposon Tn5393b from Xanthomonas campestris pv. vesicatoria, where it was shown to increase the expression of the strA-strB genes (Sundin and Bender, 1995). These observations indicate that IS6100 has been disseminated both widely and recently among very different bacterial species.
References