IS5708
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ010745 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae KIIIA |
DNA section
IS Length : 1364 bp
Ends
IR Length : 12/13
IRL : gaatggAAGGGACTTCCCCTAGATATGCGCCTGATTAATTTTCGTGCAAT
IRR : ---tatAAGGG-CTTCCCCGAACAGTCAACGCCGGTTTTTAATTTCTGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNCTTAAC | GTAGGATAAT | 0 | |
| NNNCTTAACG | TAGGATAATT | 0 |
DNA sequence
GAATGGAAGGGACTTCCCCTAGATATGCGCCTGATTAATTTTCGTGCAATCATACGGATAAACATTGACCCAGAGGGATTCGTTATGACCATCACCACTG
TCGGTATCGATCTTGCTAAAAATGTGTTTGCTGTTCACTGCATTGACCAAAATGGTAAAGCTGTTCTGGTTAAACCCAAGGTGTCGCGTGCAGCACTTTC
TGAACTCATCGCAGGTTTACCTCCCTGTGTCATTGGAATGGAAGCCTGCTCGGGTGCCCACTATTGGGCAAGATTATTCAGGCAGTATGGCCATGATGTC
CGGTTGATGGCTCCGAAATTTGTCTCACCCTATCGCATGGCGGGTAAGACCGGGAAAAATGATGCAGCTGATGCAATAGCTATTTGTGAGGCAGTGCAGC
GGCCGCATATGCGCTTTGTACCAGTCAAAGACGAAAGCCAGCAGGCAATGCAATGCTTACATCGGACAAGACAGGGATTTATCCAGGAAAGAACAGCAAC
GTATAACCGTCTTCGGGGCCTGATATCTGAATTCGGGGTTATTGCCCCACAAAGCACTGATGCTCTGCGTCATGTTGTTTCTGACCAGAAGGAAACTCTG
CCAGTACAGGTCAGGCTGTGCATTGATGATCTATTGACACATGTCACTAATATTGAAGAGAAAATCGCCGAATATGACAGGGTATTATCTCGTGTGGCCA
AAACAGATCATCGCAGCCAGCAGTTAATGGAACTGAAAGGTGTTGGGCCCACCACAGCGAGTGCGCTGGTCGCCAGTATTGGCAATGCCCATGATTTTAA
GAACGGGCGCCAACTGGCAGCCTGGTTGGGGCTAACGCCATCACAATACAGTAGTGGCGGAAAATCAAAGCTTGGCAGGATAACAAAAGCGGGTGATGCC
TATCTGCGAACCCTACTGGTCCAGGGAGCCCGTTCTGTAATGGTAGGGGCAGAGAATAGAACGGACCCGTTTAGCCGTTGGGTCTGCTCGTTGATTGGTA
GGCGGGGATACTGGCGTGCTGTCGTTGCAATCGCCGCCAAGAATGCGAGGTTGTGCTGGGCATCATTACATTACGGCGATGACTTCAGACTGTATTCAAC
AACATGAAAAATTTAACTGAGTAGTAACTGAACTGTAAATTGTTGATGGTAAAGGGTTAGACCCCCGTGAGAAGTATCTGCATATCCTACTGGATATCAC
TATCCGATTAACGAAGGAGGATCTCACGAGCGTCTTTCATCAGGGTCCGAATCACTAGCGATTCAGCATGACCGTTTATAGTAGCGCAGTCTTATCCCTT
GCCTTTCATTGATGAACAGACAGAAATTAAAAACCGGCGTTGACTGTTCGGGGAAGCCCTTATA
TCGGTATCGATCTTGCTAAAAATGTGTTTGCTGTTCACTGCATTGACCAAAATGGTAAAGCTGTTCTGGTTAAACCCAAGGTGTCGCGTGCAGCACTTTC
TGAACTCATCGCAGGTTTACCTCCCTGTGTCATTGGAATGGAAGCCTGCTCGGGTGCCCACTATTGGGCAAGATTATTCAGGCAGTATGGCCATGATGTC
CGGTTGATGGCTCCGAAATTTGTCTCACCCTATCGCATGGCGGGTAAGACCGGGAAAAATGATGCAGCTGATGCAATAGCTATTTGTGAGGCAGTGCAGC
GGCCGCATATGCGCTTTGTACCAGTCAAAGACGAAAGCCAGCAGGCAATGCAATGCTTACATCGGACAAGACAGGGATTTATCCAGGAAAGAACAGCAAC
GTATAACCGTCTTCGGGGCCTGATATCTGAATTCGGGGTTATTGCCCCACAAAGCACTGATGCTCTGCGTCATGTTGTTTCTGACCAGAAGGAAACTCTG
CCAGTACAGGTCAGGCTGTGCATTGATGATCTATTGACACATGTCACTAATATTGAAGAGAAAATCGCCGAATATGACAGGGTATTATCTCGTGTGGCCA
AAACAGATCATCGCAGCCAGCAGTTAATGGAACTGAAAGGTGTTGGGCCCACCACAGCGAGTGCGCTGGTCGCCAGTATTGGCAATGCCCATGATTTTAA
GAACGGGCGCCAACTGGCAGCCTGGTTGGGGCTAACGCCATCACAATACAGTAGTGGCGGAAAATCAAAGCTTGGCAGGATAACAAAAGCGGGTGATGCC
TATCTGCGAACCCTACTGGTCCAGGGAGCCCGTTCTGTAATGGTAGGGGCAGAGAATAGAACGGACCCGTTTAGCCGTTGGGTCTGCTCGTTGATTGGTA
GGCGGGGATACTGGCGTGCTGTCGTTGCAATCGCCGCCAAGAATGCGAGGTTGTGCTGGGCATCATTACATTACGGCGATGACTTCAGACTGTATTCAAC
AACATGAAAAATTTAACTGAGTAGTAACTGAACTGTAAATTGTTGATGGTAAAGGGTTAGACCCCCGTGAGAAGTATCTGCATATCCTACTGGATATCAC
TATCCGATTAACGAAGGAGGATCTCACGAGCGTCTTTCATCAGGGTCCGAATCACTAGCGATTCAGCATGACCGTTTATAGTAGCGCAGTCTTATCCCTT
GCCTTTCATTGATGAACAGACAGAAATTAAAAACCGGCGTTGACTGTTCGGGGAAGCCCTTATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 85 | 1107 | + | No |
Chemistry : DEDD
ORF sequence :
MTITTVGIDLAKNVFAVHCIDQNGKAVLVKPKVSRAALSELIAGLPPCVIGMEACSGAHYWARLFRQYGHDVRLMAPKFVSPYRMAGKTGKNDAADAIAI
CEAVQRPHMRFVPVKDESQQAMQCLHRTRQGFIQERTATYNRLRGLISEFGVIAPQSTDALRHVVSDQKETLPVQVRLCIDDLLTHVTNIEEKIAEYDRV
LSRVAKTDHRSQQLMELKGVGPTTASALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDAYLRTLLVQGARSVMVGAENRTDPFSRWV
CSLIGRRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSTT
CEAVQRPHMRFVPVKDESQQAMQCLHRTRQGFIQERTATYNRLRGLISEFGVIAPQSTDALRHVVSDQKETLPVQVRLCIDDLLTHVTNIEEKIAEYDRV
LSRVAKTDHRSQQLMELKGVGPTTASALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDAYLRTLLVQGARSVMVGAENRTDPFSRWV
CSLIGRRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSTT
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 6 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 5 nt on the left and 4 on the right.
Found Tn5708.
IS5708 is inserted within a putative 38 bp transposon TIR sequence, one side of the 7th base in from the end of the transposon.
The transposase protein is 88% identical to that of IS1618 from Yersinia pestis.
By analogy with IS4321, IS5708 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
Found Tn5708.
IS5708 is inserted within a putative 38 bp transposon TIR sequence, one side of the 7th base in from the end of the transposon.
The transposase protein is 88% identical to that of IS1618 from Yersinia pestis.
By analogy with IS4321, IS5708 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Albiger et al. unpublished
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384