IS5376

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
X67861 Y Bacillus stearothermophilus
Bacillus stearothermophilus T124
Bacillus stearothermophilus CU21
Bacillus stearothermophilus FH112
DNA section
IS Length : 2107 bp

Ends


IR Length : 39/50

IRL : TGTTAAAGCCGATGATAAAATCCCCAATATAGCCGGAATAAAATTCCCCA
IRR : TGTCAAGGCCGATTATTTTTTCCCCAAAATCGCCGGTTTAAAATTCCCCA

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNTGAATNNNNNNNNNN5

DNA sequence

TGTTAAAGCCGATGATAAAATCCCCAATATAGCCGGAATAAAATTCCCCACTTACAATAGAACCATAGTGTCAACGAGAGGAGCTATGGTTCATGATTAC
GAGAGGGGAATTTTTTATGATCAAAGAGATGTATGAAAGGGGAATGAGTATTTCCGATATTGCGAGGGAATTGGGGATCGATCGGAAAACCGTCCGAAAA
TATATTCACTCCCCCAATCCTCCTTCCAAATCCAAGCGAAAACAAAGAAAAAGCAAGTTAGATCCATTTAAGCCGTATCTTCAAAAACGAATGTTAGAAG
ATGGGGTGTTTAATAGCGAAAAGTTGTTTTTTGAAATTCGACAACAGGGCTATACGGGAGGAAAGACGATTTTAAAAGACTATATGAAACCTTTCCGAGA
GACGGCGAAAAAGAAATACACCGTTCGTTATGAAACGCTTCCTGGCGAACAAATGCAAGTCGATTGGAAAGAAGTTGGGGAGGTCGTGATCGAAGGGAAA
AAAGTCAAGTTATCGCTATTTGTGGCCACGTTAGGCTATTCGCGGATGAAATACGCGGTATTTACGACCAGCCAGGATCAGGAACACTTAATGGAATGCC
TGATTCAGAGCTTCAAGTACTTTGGCGGGGTTCCGAAGAAGGTGTTATTTGACAATATGAAGACCGTTACAGACGGGCGAGAACAAGGAGTGGTGAAATG
GAATCAACGATTTTCCGAATTTGCGAGTTACTATGGATTTATTCCAAAAGTATGCCGGCCTTACCGGGCCCAGACAAAGGGAAAAGTCGAACGAGCCATT
CAGTATATCATGGATCACTTCTATGTGGGGACAGCGTTTGAAAGCATCGAGGAATTAAATTTCCTTCTCCATCGTTGGCTCGATCAAGTGGCGAATCGGA
AGCCAAACGCCACTACCGGTATTTCTCCGCAAGAGCGTTGGGCCGAGGAGTCACTCAAGCCTCTTCCGTTGAAAGATTACGATACGAGCTATCTTTCCTA
TCGGAAGGTGCATTGGGATGGCAGTTTCTCCTACAAAGGGGAACAATGGCTCTTATCGGCGGAGTATGCGGGCAAAGAAATTCTGGTGAAGGAGCGATTA
AATGGAGATATTCGATTGTACTTTCGAGGGGAGGAGATTTCTCACGTGGACCAACAGAAAAAAGTGATTTCATTCGCCGAAAAAATAAAAAAGAAACAAA
CGGAAATGGCCGCCACCATTTCGCCTGTTTCGGTGGAAGTGGATACTCGTCCATTGTCCGTTTATGACGCATTCCTGCGAGGGGAAAGCTCATGAAAGAA
CGAATACACGAGTATTGCCACCGACTCCATTTGCCTGTCATGGCGGAACGATGGTCCGCCATGGCAGAATACGCCTCTACTCATAATATATCATATTCAG
AGTTTTTATTCCGCTTATTAGAGGCAGAAATCGTCGAAAAACAGGCACGATCGATCCAAACGCTCATCAAGCTGTCCAAACTGCCGTATCGCAAGACGAT
CGATACGTTTGATTTTACCGCGCAGCCTTCGGTGGATGAGCGCCGGATTCGAGAACTGCTTACGTTGTCCTTTATTGACCGGAAAGAAAATATCCTCTTT
CTCGGTCCACCGGGTATTGGGAAGACACATCTGGCAATTTCGATTGGAATGGAGGCGATCGCAAGAGGATATAAAACGTATTTTATTACCGCTCACGATT
TGGTCAATCAGTTAAGAAGAGCCGACCAGGAAGGAAAGTTGGAGAAAAAGCTTCGTGTCTTTGTGAAGCCAACCGTTCTCATTATTGATGAAATGGGGTA
TCTAAAACTGGACCCGAACAGCGCTCATTACTTATTTCAAGTGATCGCCCGGCGGTACGAGCATGCCCCGATTATCCTCACCTCCAACAAAAGCTTTGGG
GAATGGGGAGAAATCGTGGGAGACTCGGTTTTGGCGACAGCGATGTTAGATCGATTACTGCATCATTCCATCATTTTCAACCTAAAGGGGGAAAGCTATC
GATTACGGGAAAAGAGGCTCCAAGAAGAAAAACAGAAGGATCAATGAAAGGTCCTTCTGGGGAATTTTAAACCGGCGATTTTGGGGAAAAAATAATCGGC
CTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1203 bp400 aa931295+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MITRGEFFMIKEMYERGMSISDIARELGIDRKTVRKYIHSPNPPSKSKRKQRKSKLDPFKPYLQKRMLEDGVFNSEKLFFEIRQQGYTGGKTILKDYMKP
FRETAKKKYTVRYETLPGEQMQVDWKEVGEVVIEGKKVKLSLFVATLGYSRMKYAVFTTSQDQEHLMECLIQSFKYFGGVPKKVLFDNMKTVTDGREQGV
VKWNQRFSEFASYYGFIPKVCRPYRAQTKGKVERAIQYIMDHFYVGTAFESIEELNFLLHRWLDQVANRKPNATTGISPQERWAEESLKPLPLKDYDTSY
LSYRKVHWDGSFSYKGEQWLLSAEYAGKEILVKERLNGDIRLYFRGEEISHVDQQKKVISFAEKIKKKQTEMAATISPVSVEVDTRPLSVYDAFLRGESS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa12922047+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MKERIHEYCHRLHLPVMAERWSAMAEYASTHNISYSEFLFRLLEAEIVEKQARSIQTLIKLSKLPYRKTIDTFDFTAQPSVDERRIRELLTLSFIDRKEN
ILFLGPPGIGKTHLAISIGMEAIARGYKTYFITAHDLVNQLRRADQEGKLEKKLRVFVKPTVLIIDEMGYLKLDPNSAHYLFQVIARRYEHAPIILTSNK
SFGEWGEIVGDSVLATAMLDRLLHHSIIFNLKGESYRLREKRLQEEKQKDQ

 

Blast result :
Comments
At least four potential 23-bp repeats have been identified (J. Mahillon) at the ends of IS5376:
L1: TAAAGCCGATGATAAAATCCCCA (4-26)
L2: TATAGCCGGAATAAAATTCCCCA (28-52)
R1: CAAGGCCGATTATTTTTTCCCCA (2082-2104) CS
R2: AATCGCCGGTTTAAAATTCCCCA (2058-2080) CS
References
1] Xu, K., He, Z.-Q., Mao, Y.-M., Sheng, R.-Q., and Sheng, Z.-Y. (1993) Plasmid 29, 1-9.