IS4Sa

  • Family IS4
  • Group IS4Sa
MGE type ISRelated element(s) :
Isoform Synonym(s) IS4Sa1
Accession numberTranspositionOriginHost
U38915 ND Synechocystis sp.
Synechocystis sp. PCC6803
DNA section
IS Length : 1303 bp

Ends


IR Length : 15/19

IRL : TAAGAGAGTTAATTTATAATCTATTTTCATGTCCAATTTCTGTTGAACTC
IRR : TAAGGATGTTAATTTATTAATTTAAAAGGACTGAAATTTACCCTTAATAA

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TAAGAGAGTTAATTTATAATCTATTTTCATGTCCAATTTCTGTTGAACTCATCAGTTGTATGAAATCAGAAGTGTTGAACGATAGTTATAAAGAGAAAAA
AAGCCCTTTAAAATGATAGAATAAAGGGCAGAAATATTAGAATAATTGAAGAGATGATATCAAATTTTGGACATATTGTCAAAACTTATCTATCTAATTT
TCCTAAAGATGACTACCCAGTATTAGACACATTTAAGTTTGTGAGTATCTGGTTAGGATTAGTGTTAGACCAGAGCCAGACAAGCATGAGGAGTATGTTT
AAAAGACTGAACTTACGAGGAGAGACAGTCGATATATCTACATTCTCAAAGGCAAGCAAAAAAAGGGATGTGGGAGTTTTTAGAGAAATAATATTTTCCT
TAAAAAAGGAATTGTCGAAAAGGAAGGAAATTAAGCAGGGAGAATTAGAAATATTTCCTCTCGATTCAACCATTGTCAGCATAACTAGTAAGTTGATGTG
GAACCTTGGATTTCATCAGGTAAAAGTATTCAGTGGGATTAACTTATCAACAGGGATTCCGGGGGGAATAGTAATTCACTTTGGACAAGGTCATGATAAT
AAATATGGGAATGAAACAATAGAAGAGACTCCAGAAAATGGAGTAGCAGTAATGGATAGAGGATTTTGTGACTTACAGAGAATCAAGAGATTACAAAAGG
AGAATAACAAATATCATGTATTAAGAATAAAGAATAATATAAAGCTAGAAAAATTAGCCAATGATAACTATATGGTTGGAACTGGAAAGAATAAAATAGA
GAGTAGAGTGGTAATATTCACCCATGATAATTCAGAGTTTAGATTGGTTACAAATTTACCTATAGAAAGTAAAGAAATAGAAGGAGTTAGTGATGAAAAA
ATAGCAGAAATTTATAAAAAAAGATGGCAAATAGAATTGTTATGGAAATTCTTAAAAATGCACTTAAAGCTGAATAGACTGATTGCCAAAAATGAGAATG
CAATTGGGATTCAAATCTATACCTGTATAATTGCTTATTTAATACTAAAATTACTGGTAATCCCAAAAGAAGCAGGTACAACAATGTTAGACAAACTACG
TTATTTACAGGCTTTCATGTGTGAAAAAATAAGCTATGTACACTGGCTAAGGGAGTTAGCACTAAGGTGATGAATTTGCTTTGCTGTAGGATTATTCTGT
CTATTTTTAGGTCGTACATAAGTGCGATTCAACGATTCTGAATCAAAAGGTATTTATTAAGGGTAAATTTCAGTCCTTTTAAATTAATAAATTAACATCC
TTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1017 bp338 aa1541170+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISNFGHIVKTYLSNFPKDDYPVLDTFKFVSIWLGLVLDQSQTSMRSMFKRLNLRGETVDISTFSKASKKRDVGVFREIIFSLKKELSKRKEIKQGELEI
FPLDSTIVSITSKLMWNLGFHQVKVFSGINLSTGIPGGIVIHFGQGHDNKYGNETIEETPENGVAVMDRGFCDLQRIKRLQKENNKYHVLRIKNNIKLEK
LANDNYMVGTGKNKIESRVVIFTHDNSEFRLVTNLPIESKEIEGVSDEKIAEIYKKRWQIELLWKFLKMHLKLNRLIAKNENAIGIQIYTCIIAYLILKL
LVIPKEAGTTMLDKLRYLQAFMCEKISYVHWLRELALR

 

Blast result :
Comments
IS4Sa is located 3 kb downstream of an lytB-like gene and 1.4 kb from IS5Sb (Cassier-Chauvat et al., 1996). The IS5Sa copy has been inactivated by the insertion of a mariner-like element that has duplicated 2 bp. Therefore, the proposed IS5Sa is a "reconstructed" element, based these assumptions. However, the exact limits of IS5Sa remain to be confirmed. IS5Sa putative transposase is 26 % identical to that of IS5377 from Bacillus stearothermophilus (in a 301-aa overlap).
There are 11 copies of IS4Sa in the Synechocystis genome.
References
1] Cassier-Chauvat, C., Poncelet, M., Villoing, S., and Chauvat F. "The nature of three insertion sequences from the cyanobacterium Synechocystis PCC6803 supports the hypothesis of trans-phylla and trans-kingdom gene transfers" (1996) EMBL Data Library, Rel. 47.