IS493
- Family IS5
- Group ISL2
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
M28508 | Y | Streptomyces lividans | Streptomyces lividans CT2 |
DNA section
IS Length : 1643 bp
Ends
IR Length : 21/24
IRL : GAGCGTTTTTCAACCTCAGGCTGACGGGGTCAGGGGTCAGAGGCGGGGTC
IRR : GAGCGTTTTCCATCCTCAAGCTGAGCTGGCCTGATGCAGGCAGAGGACGG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
ATCGCTAGTC | AGT | GGCCTGNNNN | 3 |
AATCGGTGTC | AAT | GGATAACTGA | 3 |
GGTTGTCGAC | ACT | AAGTCGGGCA | 3 |
CTTCCAGACC | ACT | CTGTCCAAGT | 3 |
CGGTTAAGGC | ACT | GCATTAACGG | 3 |
ATCGCTAGTC | AGT | GGCCTGAAGA | 3 |
DNA sequence
GAGCGTTTTTCAACCTCAGGCTGACGGGGTCAGGGGTCAGAGGCGGGGTCTGGATCAGCGCGAGCGGTCGTGAGGTAGTACCGGTTGCGCTCGAAGCTCA
CTGCCGTCTAGGACAGAAGCTGACCCTAGCTCACGCCTACCGTCGCTGCTGGCAAGCAACATCAGCGAAAGGACCGCGATGCCCCAGCAGACCAGCCCCG
AGCCGGAGGGCTACACCACCGTTGCGCCCTGGGTCGTCACCGACGACACGGGGGCTTTCCTCGACTTCGTCACCCAGGCATTCGGGGGCGAGGAACTCGG
GCGGGTGTCGACCGAGGAGGGCTTGATCGGTCACGCTGAGATCCGAGTCGGCGACACCGTCGTGTTGGCCTTCGACCGACACACGGACTGGCCCGCCATG
CCGAGCCTGCTGCGCGTATTTGTCGCCGATGCGGACGAGGCGTTTTCCCAGGCCGTCGCAGCTGGCGGCTATGTCGTCACTTCCCTGGCCGACGACGCCT
TCGGACAGCGCGGAGGGCGCATCAAGGACCCCTTCGGCAATATCTGGTGGGTGGTCAGCTGCGTTGAGGACGTCTCTGAGGGCGAGATGTGGAAGCGGCT
GCAGGACCCCGTATACGCCGAGGCCATGCGCGTGGCTCAGGAGACACTCGACGCCGAGCTCAGCGGTCGACGCCACGGGCGCAGCAGCGCACCCGTCAGG
ACAACCAGCTAGACCCACGACGTCGGGGTTCGCATGCGCCAGACCGGTAGCCAAGGAGCGTCTTCATTCTGCTTCGGCAGCTTGCGGGTTGGCCTGACAC
AGGGGTGAAGCCCCTGGTAGATGGGTTTTCGACCAAGAAATCCGTCTCTACTAGAGGCTTCACGTGCTTGTTTACCCCTCCGGGCTGGACGTGTCCAGTT
CTGCCCTCCGCTTCCTCTCCGCCCGTCTGCGGGAACACCGCCGTGGTCTCGGCACCCGGTGGCGGCGCCTGAGCGCAGGTCGGCAGGCTCTGCTCGCCCT
CGCCCATCTCCGCAACGGTCATCCGTATGTGCAGCTCGCGGCCGGGTTCGGCGTGGGCACCACCACTGCCTACCGCTACGTCACCGAGGCAGCTGAGGTT
CTGGCCGCCCTTGCCCCGACGCTCGCCGAGGCGGTCCGGGCGGCGTCGATGAAGGCGTTCGTCCTGCTGGACGGGACGCTGCTGCCGATCGACCGAATCG
CCGCCGACCGGCCTTTCTACTCGGGCAAGCACAAGAAACACGGGATGAACGTACAGGTGATCGCAGATCCTTCCGGCCGCCTGCTGTGGGCCTCGCCGGC
CCTGCCCGGAGCCGTCCACGACGTCCGTGCGGCCCGCGAGCACGGCATCATCGACACGCTTGCCACAGCCGACGTCAACTGCTGGGCAGACAAGGGCTAC
CAGGGCGCCGGAGGCACGGTCCGTGTTCCCTACCGCGGCCGGTGGGAGACGCTCTCTGCAGGGCAGCAGGCCGTCAATCGATCCCACGCGAAGATCCGGG
CGCTCGTCGAGCAAGCCGTCGCGACCCTCAAGTCCTGGCGACTCCTTCGCAGGCTCCGTTGCTCGACCACCCGCGTCACGGTCCTCGTCCAGGCCGTCCT
CTGCCTGCATCAGGCCAGCTCAGCTTGAGGATGGAAAACGCTC
CTGCCGTCTAGGACAGAAGCTGACCCTAGCTCACGCCTACCGTCGCTGCTGGCAAGCAACATCAGCGAAAGGACCGCGATGCCCCAGCAGACCAGCCCCG
AGCCGGAGGGCTACACCACCGTTGCGCCCTGGGTCGTCACCGACGACACGGGGGCTTTCCTCGACTTCGTCACCCAGGCATTCGGGGGCGAGGAACTCGG
GCGGGTGTCGACCGAGGAGGGCTTGATCGGTCACGCTGAGATCCGAGTCGGCGACACCGTCGTGTTGGCCTTCGACCGACACACGGACTGGCCCGCCATG
CCGAGCCTGCTGCGCGTATTTGTCGCCGATGCGGACGAGGCGTTTTCCCAGGCCGTCGCAGCTGGCGGCTATGTCGTCACTTCCCTGGCCGACGACGCCT
TCGGACAGCGCGGAGGGCGCATCAAGGACCCCTTCGGCAATATCTGGTGGGTGGTCAGCTGCGTTGAGGACGTCTCTGAGGGCGAGATGTGGAAGCGGCT
GCAGGACCCCGTATACGCCGAGGCCATGCGCGTGGCTCAGGAGACACTCGACGCCGAGCTCAGCGGTCGACGCCACGGGCGCAGCAGCGCACCCGTCAGG
ACAACCAGCTAGACCCACGACGTCGGGGTTCGCATGCGCCAGACCGGTAGCCAAGGAGCGTCTTCATTCTGCTTCGGCAGCTTGCGGGTTGGCCTGACAC
AGGGGTGAAGCCCCTGGTAGATGGGTTTTCGACCAAGAAATCCGTCTCTACTAGAGGCTTCACGTGCTTGTTTACCCCTCCGGGCTGGACGTGTCCAGTT
CTGCCCTCCGCTTCCTCTCCGCCCGTCTGCGGGAACACCGCCGTGGTCTCGGCACCCGGTGGCGGCGCCTGAGCGCAGGTCGGCAGGCTCTGCTCGCCCT
CGCCCATCTCCGCAACGGTCATCCGTATGTGCAGCTCGCGGCCGGGTTCGGCGTGGGCACCACCACTGCCTACCGCTACGTCACCGAGGCAGCTGAGGTT
CTGGCCGCCCTTGCCCCGACGCTCGCCGAGGCGGTCCGGGCGGCGTCGATGAAGGCGTTCGTCCTGCTGGACGGGACGCTGCTGCCGATCGACCGAATCG
CCGCCGACCGGCCTTTCTACTCGGGCAAGCACAAGAAACACGGGATGAACGTACAGGTGATCGCAGATCCTTCCGGCCGCCTGCTGTGGGCCTCGCCGGC
CCTGCCCGGAGCCGTCCACGACGTCCGTGCGGCCCGCGAGCACGGCATCATCGACACGCTTGCCACAGCCGACGTCAACTGCTGGGCAGACAAGGGCTAC
CAGGGCGCCGGAGGCACGGTCCGTGTTCCCTACCGCGGCCGGTGGGAGACGCTCTCTGCAGGGCAGCAGGCCGTCAATCGATCCCACGCGAAGATCCGGG
CGCTCGTCGAGCAAGCCGTCGCGACCCTCAAGTCCTGGCGACTCCTTCGCAGGCTCCGTTGCTCGACCACCCGCGTCACGGTCCTCGTCCAGGCCGTCCT
CTGCCTGCATCAGGCCAGCTCAGCTTGAGGATGGAAAACGCTC
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
765 bp | 254 aa | 864 | 1628 | + | No |
Chemistry : DDE
ORF sequence :
MLVYPSGLDVSSSALRFLSARLREHRRGLGTRWRRLSAGRQALLALAHLRNGHPYVQLAAGFGVGTTTAYRYVTEAAEVLAALAPTLAEAVRAASMKAFV
LLDGTLLPIDRIAADRPFYSGKHKKHGMNVQVIADPSGRLLWASPALPGAVHDVRAAREHGIIDTLATADVNCWADKGYQGAGGTVRVPYRGRWETLSAG
QQAVNRSHAKIRALVEQAVATLKSWRLLRRLRCSTTRVTVLVQAVLCLHQASSA
LLDGTLLPIDRIAADRPFYSGKHKKHGMNVQVIADPSGRLLWASPALPGAVHDVRAAREHGIIDTLATADVNCWADKGYQGAGGTVRVPYRGRWETLSAG
QQAVNRSHAKIRALVEQAVATLKSWRLLRRLRCSTTRVTVLVQAVLCLHQASSA
Blast result :
Comments
IS493 transposase is 36 % identical to that of IS1373. Although two ORF are present within IS493, 177 (179-712) and 254 (864-1628), the second one has been shown to be the actual transposase (Baltz et al., 1992). This IS might just be a complex (rearranged) element. Several vectors have been derived from IS493: Tn5096 to Tn5099 (xylE promoter probe) and Tn5099-10, a derivative that transposes at a 1000-fold higher frequency, due to a change in its left IR (Solenberg and Baltz, 1994). The target consensus results from the analysis of about 15 different sites.
References
1] Solenberg, P.J., and Burgett, S.G. (1989) J. Bacteriol. 171, 4807-4813.
2] Solenberg, P.J., and Baltz, R.H. (1991) J. Bacteriol. 173, 1096-1104.
3] Hahn, D.R., Solenberg, P.J., and Baltz, R.H. (1991) J. Bacteriol. 173, 5573-5577.
4] McHenney, M.A., and Baltz, R.H. (1991) J. Bacteriol. 173, 5578-5581.
5] Baltz, R.H., Hahn, D.R., McHenney, M.A., and Solenberg, P.J. (1992) Gene 115, 61-65.
6] Solenberg, P.J., and Baltz, R.H. (1994) Gene 147, 47-54.
2] Solenberg, P.J., and Baltz, R.H. (1991) J. Bacteriol. 173, 1096-1104.
3] Hahn, D.R., Solenberg, P.J., and Baltz, R.H. (1991) J. Bacteriol. 173, 5573-5577.
4] McHenney, M.A., and Baltz, R.H. (1991) J. Bacteriol. 173, 5578-5581.
5] Baltz, R.H., Hahn, D.R., McHenney, M.A., and Solenberg, P.J. (1992) Gene 115, 61-65.
6] Solenberg, P.J., and Baltz, R.H. (1994) Gene 147, 47-54.