ISfinder Mirror Site

IS4811

Family IS5
Group IS1031

MGE type ISRelated element(s) :
Isoform Synonym(s) Tn4811, IS456

Accession number	Transposition	Origin	Host
Z11519	Y	Streptomyces lividans	Streptomyces lividans ZX7 Streptomyces lividans TK64 Streptomyces lividans 1326 (SLP2)

DNA section

IS Length : 5396 bp

Ends

IR Length : 9/11

IRL : GTAGCTGTTTCTGATCCCCGGTTCTGAGTAGCCTTGCGTCCTAGGCTGGC
IRR : GAAGTTGTTTCCGAAGTAGGCGCCCTCACGGTGGTTTAGGAGCCGGAGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
NNNNCGGGCG	TGA	GCGAACTGCC	3
NNNNTCGGGC	TGA	CGCTTGACGC	3

DNA sequence

GTAGCTGTTTCTGATCCCCGGTTCTGAGTAGCCTTGCGTCCTAGGCTGGCGTGGTGTCCAGGGGTACCGGGCGGGCCGGGGAAAGGGACTGTCGGGCTTG
TGGCGAAGGGCTGAGGCCGGGTTCGCGGCCTGATGCGCTGTTCTGTTCCTCGGCATGTCGTGCCAGGCAGTGGCGGGCGGAACGCCGGTTACGCAAGCGC
CTGGCGGCCGTCCGGGGCGGTGCCGGTGAGGTGGAGTGTCCCGAGTGCGGGGTCCGCTGGGTCGTCGGCGTCGATCGGCGCTCGGACGCCGTCTACTGCT
CGCGCCGGTGTGTGGTGAGGGCCTGGAGGCGCCGGAAGGAAACGTTCGGCGAACGGGCACAGTGACGGCAACGCGAACACATCCTGAGTTCAAGTTTGGT
CAGAAACTGGACATCCGTGGTCATTCGTTGTGATCAGTGGATTTACGGTCTGACTCTCGGTGATGAGCGGGTCGTGTCCGCGTCACGGACGTCAGCTCCG
TGCTGCGGCCCGTATGGGTGGTGGAACACCATGCCGGAGGAGATCGACAGGGTTGGAAGTGTCAGTCAGCGCCGCTACGAGCAGATCGTGGCCGAGTTGC
GGGAAGTGGTCGAACAGCAGACACAGGGCTCGTTCACGATCGGGGACCGCGCGCTGGAGATCGAGCCGATGCGTGAGCGGGGCGGTGGCCAGCAGGTTGC
TCCGGGCCAGGAGTTGTTCACGGTCAGCGAGACGCTGCACCGTCTTGCCGAGGACATCGGACTGGCCTACAGGACCGTGGAAAAGGCGAGGTGGACGGCG
TCTCGGTGGCCGAAGGACAAGCGGCAGAAGGGCGTGTCGTTTCGGGTCCACAGAGTCCTGGCCCAGATCGCGGATGAGGCTGAGCGGTTCGCGACCATCG
CCAAACCCCCCGCGGGCAAGACGCGGTGGACGGGGGACGAGGCCAACCGCAAGGTGGGTCGCCAGGTCGAGCGTCCGGCATCGCCTCAGGAGAAGATTTC
CGCGATCCACCATCTGGCCCGGGACGAGGACGTCGCTGCGGTGGTGACCAGCGACTTCCTCAAACGTCCGACGGTGGCGGCCAAGGTCTCCGACCAGGAC
AAGGTCCGGGTGGTGGAGGAGTTCACCCGCGACGAGCGCGTCGCCAGCCAGGTGACCACCGGTCTGCTGCGTCGCCCTGAAGTGGCGTACAAGGCAATGA
GCGACGACACCGCCCGCCACCAGGTCAATCAGGCTCAGGTCGAGCGAGGTCGGCAGGCCCGCGAGCACTTCGAGGACACCAATCCGGTTGCCCCGGCGGT
CCGTCACATCGACCGGACGGTGGAGTTCCTGGACCTGGTCACCGCCTGTCACTCGTTCGTGGCCGCGGCCGGCCGGGCGGTCCCCGGGCTGCGTGATCGC
ACCCTGGGCGAGGATGAACGCACCATTGTCCACGAGAACGTGGCGAAGGTACGGGCGACGCTCGACTGGATCGAGACCGCGGTCGACACCGGCAAGGTCG
ACATGGACGGCGAACTGGCTCGCATGCTGCGAGGCGAGTAGCCGTGCCTCGCTCACGGCGCAAGGGAGATGCCGCCCGACGGCATGCGGACACCATCCGG
TTCGTTCTGTTCGAGGCCAGGCCGGCCGGCTCTGCGATGCACCAGCTGATCAGGGCCAGCGGGTTGTCGGACAGTCAGGTCAGATCGGGGCTGTCGGCCC
TGCGCGACGAGGCGGCGGCCAAGGGCTGGCCACCGCTGATCTGGACCCGGACCGACGGCTATCAGCTCGACGCTGAACGGGCGGCGCTGGAGTCATACGA
ACGGGCGGTGGTCAGGGAGAAGCTGACCCAGTTCCGCCGGTTCATCACCGGGACCGTCACCCCGCATGCCGCGGCCCACCCGAACGACAAGTGGGTCAAG
CACATCGTCGCTCAGCTCAACTCCATCGAATCCACCCTCGACCTCATCGCCAGTGCCTGAGCCTTCCTCGCGGGGGCCGACCTGCTCTGCCGGTCGGCCC
CCGCCGCAGGTCAGTGACGGGAGGAGAAATGCGTCACCGCCTCTATCCCTCGGACATGACGGACGCAGAGTGGGCACTGGTCGAACCGCTGCTGCCGCCG
CCGGCCTGTGACACGGCCCGCGGCGGGCGGCCGGAGAAGCATCCCCGGCGCGAGATCGTCGACGCGATTCGATACGTTGTGGACACCGGCTGCAAATGGA
GGGCCCTACCTGCGGACTTCCCTCCATGGAGGACAGTCTGGGGCTTCATGGCCCGCTGGGCGGCGGTCGGAGTCATCGGCCAGCTCCGTGACGCCCTGGC
CCAACGAATTCGCCGCGACATGGGCCGAGGGCCAAGAGCCGTCGCGACGATCATCGACTCTCAGTCGGTGAAAGCCGCCTCGACCGTCGGCAAGGACAGC
CGCGGCTATGACGCGGGCAAAAGAATCAACGGGCGCAAACGGCACATGGTGGTCGACACCAAGGGGCTGCCGCTGATGGTGATGGTCACCCCGGCCGACC
TGCACGACTCCGCCGTCGCCAAGGAAGTCCTCTTCCGACTTCGCCTGACGCACCCCGAGATCACCCTCGTCTGGGCCGACTCCGCCTATGCGGGCAAGCT
CGTGACCTGGGCAAAGAAGCATCTGAACCTCACGATCAAGACCGTCAGCCGCCCGAAGGACACCTCGGGCTGGGTTCTGTTGCCCCGCCGCTGGGTGGTC
GAACGGAGCCTGGCCTGGATGATGAACGCCCGCCGTCATGCCCGAGATTACGAGCGGCTGATCCAGCACTCCGAGGCCCTGATCACCTGGGCAGCGATCA
CGGTGATGACCAAGCGTCTGACCCGCACGGGTCCCACCGGCTGGTCGAAGAAGCCGAAGGCGACAGCCGACTCATCCCCGCAGGTCAGTACTGCTGCCAC
CGCTGGGAGGCCCGCCGGTTCAGCCACTGAAGAGCCAGCTCGAGCTGCCCCGCTGTCAGCCCGGCAGCCTCAGCCAGCCGGTCGGTTCTGAGCCCGTCGG
GATACTTGTCGAGCACCCGGGCGATCTTGCCGATCCATAGTTCTTCCTCGATGAAGGGCCGACGCGCATACCGCTGCCGGAGGACGTCCAGATGCTCCTG
GGAGCATGCGGTGACGAGGCGCAGACCGTCATGTGCCAGGTCATGCACAGAGACCGCCGACGAGTCCGGCACCGCATACCGCATTCGCCGGCCTTCGGCG
ATGGCCTGCTGGCACACGTCGCACACCACGTTGTCCCCCGTCGGGTCCTCGCACGCTCGCCTGGATTCCACCGCGGCGGCCGGTCATCCGGCGCTGAGGG
TGTCAGCGCCGTACTCCCAGAGCCGGTCTGCTGCCTCCGGGTCGAGTGCGTGGGCGGCTACGCCGCCGGGCTGGACGTCCCCGTCCTCGACGGTCCGGGC
CTCCTGGTTGTCTTCGAAGTAGCGGCCGGTGACGCCGTTGAGGAGAGGAGAGGCCGCCAGCAGGACGGAGGTGGCCGCCCCCTGTTCGGGAGTCTTGTAG
TACGGCAGCGGCTTCACGTTGCCCTGGTCGTCCATCACGCCGAATGCGCGCATGGTCTCGTCGTCGACGTGCCGCTGCAGACGGGTGAGGATGTAGCCGG
GGTTGAGCGCGTTGGCCGTGATCCCGTCGGCTGCCCAGCGGCGGGCACCCACCGTGAACAGGACGTCGGCGGTCTTGGACTGCCCGTAGGCCGCCCAGGG
GTCGTACGGCCGCCGTGCGAAATGAGGATCTTCGAAGTCGAAGGGCGTGCCCAGGTGGGCGCCGGAGCTGACGACCACGATGCGGGCGGAGCCGGCGTCC
CGCAGAGCCGCGTGCAGGCCAGTGGCCAGAGCGAAGTGGCCGAGGTAGTTGGTGGCAAGCTGCATTTCCCAGCCGTACGGGGTGAGGGTGCGTGTGGGAA
GGGCCATGATCCCCGCGTTCGCGACGAGGATGTCGAGAGGCCCGCGCCATGCCCGCGCGAAGCTGTCGACGGAGGCCACATCGGAAAGATCGAGGGCCTC
TGCGTGCACCCGGCCCGCACCGGCGGCGGCCGCCTCCTGGACCAGGGGCTCGGCGGACTGCGGGTGGCGCGTGGCGATGGTGACCTCCGCGCCGGCGGCG
GCCAGGGCCCGGACGGTTTCGGCGCCGAGACCCGAGGCACCGCCGGTGACCACGGCACGGCGGCCGGTGAGATCCACGCCGTCGATCACCTCCTGCGCGC
GGGCGTGAGAGTCGAAGGGGGTAGTCAGGAGGGCGGAGTCGTTTCGCGCGTGCCTGCCGCCGTCGGGATTCGTCGTCATGCGTCCACGCTACGATGAGGC
GACTGGATGCTCTATGACTGAGAGTCCTTCTCCTTTTAGCGATCGTCCAATCCGGGAGGGTGAGTGTGGATCCTCTTGAGGATGTACTGACCCTGCTGAA
GACCCGCAGCCATCTCTCAGCGAGCCTGGTCGCGGGAGGACGCTGGGCCGTGCGGTTCGACGCCCCCCGCGTGGTGAAGTTCAATGCCGTTCGCCGCGGC
ACCTGCCAGCTGGAGGTCGACGGGATCGACGAGCCGATCGACCTGGCCGAGGGTGACTGCTACCTCCTGACACGGCCACGCTCCTTCACGCTCCGCAGCG
ATCCGGAAACTGCTCCTGTCGATGGCGGAGTCGTGTTCGCAAGAGCCGAAGACGGCATCGCCAGAGCGGGCCAGGGCGACGACGTGTTCCTCATCGGCGG
CGGCTTCTCCTTCGGCACCCGCGCCCAGGAGCTGCTGCTCGACAGGCTGCCCCCGATCGTCCATGTGCCCGCCGACACCCCGCACGCGGAGACGGTGCAG
TGGGCACTGACCGCCATCGACCAGGAGCTGACACATCGGCCAATGGCCTCCACCCTGATCGCGGAGCACCTGGCCGTCATCATGCTCGTTCATGTGCTGC
GCCTGCACCTCGAACGCGCGCCGCACGCGGTGTCAGGCTGGCTGGCCGGCCTCGCAGATCCCGTGGTCGCCACAGCGCTGACCTGCCTGCACCGCGATCC
GGCGCGTTCTTGGACCGTGGCCGACCTGGCCGACACCGCCGCGGTGTCCCGTTCCACCCTGGCCGCCCGCTTCAAAGCCACAGTCGGCCAAGGGCCATTG
GAATACCTCACGCGATGGCGGATCGAGCTCGCCGCCCGCCAGCTACGGGAAGGCAACGCAACACTCGCCTCCATCGCCCACTCCGTGGGATACGGATCCG
AAAGCGCCCTCAGCGTCGCCTTCAAAAGGGTCCTGGGAATGCCGCCCGGCGACTACCGCAAACATCCCACGATGCCTTGATGCTTCGAGGAGACGCGTTA
CCACAGCCGGGCAACCGATGCACTCTGCATCACCATGCATGACTATCGCTCCGGCTCCTAAACCACCGTGAGGGCGCCTACTTCGGAAACAACTTC

Protein section

ORF number : 5

ORF 5

Length		Begin	End	Strand	Fusion ORF
915 bp	304 aa	4366	5280	+	No

ORF function : Passenger Gene
Annotation : Transcription activatorDescription : Transcriptional Regulator factor

ORF sequence :

MDPLEDVLTLLKTRSHLSASLVAGGRWAVRFDAPRVVKFNAVRRGTCQLEVDGIDEPIDLAEGDCYLLTRPRSFTLRSDPETAPVDGGVVFARAEDGIAR
AGQGDDVFLIGGGFSFGTRAQELLLDRLPPIVHVPADTPHAETVQWALTAIDQELTHRPMASTLIAEHLAVIMLVHVLRLHLERAPHAVSGWLAGLADPV
VATALTCLHRDPARSWTVADLADTAAVSRSTLAARFKATVGQGPLEYLTRWRIELAARQLREGNATLASIAHSVGYGSESALSVAFKRVLGMPPGDYRKH
PTMP

Blast result :

ORF 1

Length		Begin	End	Strand	Fusion ORF
1011 bp	336 aa	531	1541	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPEEIDRVGSVSQRRYEQIVAELREVVEQQTQGSFTIGDRALEIEPMRERGGGQQVAPGQELFTVSETLHRLAEDIGLAYRTVEKARWTASRWPKDKRQK
GVSFRVHRVLAQIADEAERFATIAKPPAGKTRWTGDEANRKVGRQVERPASPQEKISAIHHLARDEDVAAVVTSDFLKRPTVAAKVSDQDKVRVVEEFTR
DERVASQVTTGLLRRPEVAYKAMSDDTARHQVNQAQVERGRQAREHFEDTNPVAPAVRHIDRTVEFLDLVTACHSFVAAAGRAVPGLRDRTLGEDERTIV
HENVAKVRATLDWIETAVDTGKVDMDGELARMLRGE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
417 bp	138 aa	1544	1960	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPRSRRKGDAARRHADTIRFVLFEARPAGSAMHQLIRASGLSDSQVRSGLSALRDEAAAKGWPPLIWTRTDGYQLDAERAALESYERAVVREKLTQFRRF
ITGTVTPHAAAHPNDKWVKHIVAQLNSIESTLDLIASA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
963 bp	320 aa	2029	2991	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRHRLYPSDMTDAEWALVEPLLPPPACDTARGGRPEKHPRREIVDAIRYVVDTGCKWRALPADFPPWRTVWGFMARWAAVGVIGQLRDALAQRIRRDMGR
GPRAVATIIDSQSVKAASTVGKDSRGYDAGKRINGRKRHMVVDTKGLPLMVMVTPADLHDSAVAKEVLFRLRLTHPEITLVWADSAYAGKLVTWAKKHLN
LTIKTVSRPKDTSGWVLLPRRWVVERSLAWMMNARRHARDYERLIQHSEALITWAAITVMTKRLTRTGPTGWSKKPKATADSSPQVSTAATAGRPAGSAT
EEPARAAPLSARQPQPAGRF

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
894 bp	297 aa	4177	3284	-	No

ORF function : Passenger Gene
Annotation : NADP-dependent oxidoreductaseDescription :

ORF sequence :

MDLTGRRAVVTGGASGLGAETVRALAAAGAEVTIATRHPQSAEPLVQEAAAAGAGRVHAEALDLSDVASVDSFARAWRGPLDILVANAGIMALPTRTLTP
YGWEMQLATNYLGHFALATGLHAALRDAGSARIVVVSSGAHLGTPFDFEDPHFARRPYDPWAAYGQSKTADVLFTVGARRWAADGITANALNPGYILTRL
QRHVDDETMRAFGVMDDQGNVKPLPYYKTPEQGAATSVLLAASPLLNGVTGRYFEDNQEARTVEDGDVQPGGVAAHALDPEAADRLWEYGADTLSAG

Blast result :

Comments

ORF1 [336 (531-1541)] contains a consensus ATP/GTP-binding motif; ORF2 [138 (1544-1960)] function remains unknown; ORF3 [320 (2029-2991)] is the putative transposase; ORF4 [297 (3284-4177) CS] codes for an oxidoreductase-like protein; and ORF5 [304 (4366-5280)] product is similar to the bacterial transcription regulators of the AraC family. One IS4811 copy is located about 10 kb from the right end of the linear plasmid SLP2, and the same configuration is found at the ends (telomeres) of the Streptomyces lividans linear chromosome molecule (Lin et al., 1993).

References

1] Tanaka, M., Tanaka, H., Kato, F., and Koyama, Y. (1988) Abstr. 7th Int. Symp. Biol. Actinomycetes. Academic Societies Japan, Tokyo. Abstr. P5-20.
2] Tanaka, M., Takabe, N., Kato, F., and Koyama, Y. (1991) Abstr. 8th Int. Symp. Biol. Actinomycetes. University of Wisconsin, Madison. Abstr. P1-056.
3] Chen, C.W., Yu, T.-W., Chung, H.-M. and Chou, C.-F. (1992) J. Bacteriol. 174, 7762- 7769.
4] Lin, Y.-S., Kieser, H., Hopwood, D.A., and Chen, C.W. (1993) Mol. Microbiol. 10, 923-933.
5] Yu, T.-W., and Chen, C.W. (1993) J. Bacteriol. 175, 1847-1852.