IS4351
- Family IS30
- Group
Isoform Synonym(s) IS4400, IS4351L, IS4351R
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M17124 | Y | Bacteroides fragilis | Escherichia Porphyromonas gingivalis 33277 Escherichia coli C600 Bacteroides fragilis V479-1 plasmid pBF4 Bacteroides fragilis V598 Bacteroides fragilis plasmid pBFTM10 Bacteroides fragilis 638 plasmid pBI136 Bacteroides ovatus 0038, Bacteroides thetaiotaomicron 5482 Bacteroides uniformis 0061 |
DNA section
IS Length : 1155 bp
Ends
IR Length : 20/25
IRL : CTTGAGTTCAACTTATAAATGCAACTTTTTGGGTGCGGATAATAAGCAAT
IRR : GCTGAATTCAACTTGCAAATGCAACAGAATTCTGATTAATAATTTGTTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTCAA | TAT | TATTGA | 3 |
| NNNNNN | ACT | NNNNNN | 3 |
DNA sequence
CTTGAGTTCAACTTATAAATGCAACTTTTTGGGTGCGGATAATAAGCAATAAAAACATTTATTTTTCAGAGAGGAAAGAGAGACAATGTCCCCCTTTCTC
TCACTCTGAATGGATAAAGTTTGCTATCTTTGCTTTAATTGTCCGTCCAAAGAAAAAAAAGTTGCAGATGAGCAAACATATAACCGAGGAACAAAGGTAT
GCAATTTCTATGATGTTGCAAATACCGATGAGCAAAAAAGCAATAGCGGAAGCTATCGGAGTAGATAAAAGCACTGTTTACAGGGAGATAAAGCGCAATT
GCGACGCCCGAAGTGGTAGCTATAGCATGGAGCTTGCCCAGCGAAAAGCAGACAGGCGCAAGCAGCAAAAACATCGCAAGGAAGTGCTTACACCGGCAAT
GAGAAAACGGATAATAAAGCTGTTGAAGAAAGGATTCAGCCCGGAGCAGATTGTCGGCAGGAGCCGCTTGGAGGGAATTGCGATGGTATCTCACGAAACG
ATATATCGCTGGATTTGGGAGGATAAGCGGCGGGGTGGCAAACTGCACAAATATCTTCGCAGACAAGGTCGCAGGTATGCCAAACGTGGTTCTAAAAATG
CAGGGCGAGGATTTATCCCAGGCAGGGTGGATATTGATGAGCGTCCCGAGATAGTGGAACTGAAGGAGAGATTTGGTGATTTAGAGATAGATACAATTAT
TGGTAAGAACCACAAAGGTGCCATTCTTACCATTAACGACAGAGCAACAAGCAGGGTCTGGATACGCAAGTTGTCGGGAAAAGAAGCCATCCCGGTAGCT
AAGATTGCAGTATGGGCACTGCGGAAAGTGAAAAACTTAATACACACAATTACGGCTGACAATGGAAAGGAGTTTGCAAAGCACGAGGAAATTGCGCAAA
AATTGGAAATAAAATTCTATTTTTGCAAACCATACCACTCATGGGAACGTGGTGCCAATGAAAACACCAACGGGCTTATCAGGCAGTATATCCCAAAGGG
TAAGGACTTTAGTGAAGTAACCAACAAACAGATTAAGTGGATTGAAAATAAACTCAATAATCGACCTCGTAAAAGACTTGGATACCTCACGCCAAACGAA
AAATTTAAACAAATTATTAATCAGAATTCTGTTGCATTTGCAAGTTGAATTCAGC
TCACTCTGAATGGATAAAGTTTGCTATCTTTGCTTTAATTGTCCGTCCAAAGAAAAAAAAGTTGCAGATGAGCAAACATATAACCGAGGAACAAAGGTAT
GCAATTTCTATGATGTTGCAAATACCGATGAGCAAAAAAGCAATAGCGGAAGCTATCGGAGTAGATAAAAGCACTGTTTACAGGGAGATAAAGCGCAATT
GCGACGCCCGAAGTGGTAGCTATAGCATGGAGCTTGCCCAGCGAAAAGCAGACAGGCGCAAGCAGCAAAAACATCGCAAGGAAGTGCTTACACCGGCAAT
GAGAAAACGGATAATAAAGCTGTTGAAGAAAGGATTCAGCCCGGAGCAGATTGTCGGCAGGAGCCGCTTGGAGGGAATTGCGATGGTATCTCACGAAACG
ATATATCGCTGGATTTGGGAGGATAAGCGGCGGGGTGGCAAACTGCACAAATATCTTCGCAGACAAGGTCGCAGGTATGCCAAACGTGGTTCTAAAAATG
CAGGGCGAGGATTTATCCCAGGCAGGGTGGATATTGATGAGCGTCCCGAGATAGTGGAACTGAAGGAGAGATTTGGTGATTTAGAGATAGATACAATTAT
TGGTAAGAACCACAAAGGTGCCATTCTTACCATTAACGACAGAGCAACAAGCAGGGTCTGGATACGCAAGTTGTCGGGAAAAGAAGCCATCCCGGTAGCT
AAGATTGCAGTATGGGCACTGCGGAAAGTGAAAAACTTAATACACACAATTACGGCTGACAATGGAAAGGAGTTTGCAAAGCACGAGGAAATTGCGCAAA
AATTGGAAATAAAATTCTATTTTTGCAAACCATACCACTCATGGGAACGTGGTGCCAATGAAAACACCAACGGGCTTATCAGGCAGTATATCCCAAAGGG
TAAGGACTTTAGTGAAGTAACCAACAAACAGATTAAGTGGATTGAAAATAAACTCAATAATCGACCTCGTAAAAGACTTGGATACCTCACGCCAAACGAA
AAATTTAAACAAATTATTAATCAGAATTCTGTTGCATTTGCAAGTTGAATTCAGC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 981 bp | 326 aa | 168 | 1148 | + | No |
Chemistry : DDE
ORF sequence :
MSKHITEEQRYAISMMLQIPMSKKAIAEAIGVDKSTVYREIKRNCDARSGSYSMELAQRKADRRKQQKHRKEVLTPAMRKRIIKLLKKGFSPEQIVGRSR
LEGIAMVSHETIYRWIWEDKRRGGKLHKYLRRQGRRYAKRGSKNAGRGFIPGRVDIDERPEIVELKERFGDLEIDTIIGKNHKGAILTINDRATSRVWIR
KLSGKEAIPVAKIAVWALRKVKNLIHTITADNGKEFAKHEEIAQKLEIKFYFCKPYHSWERGANENTNGLIRQYIPKGKDFSEVTNKQIKWIENKLNNRP
RKRLGYLTPNEKFKQIINQNSVAFAS
LEGIAMVSHETIYRWIWEDKRRGGKLHKYLRRQGRRYAKRGSKNAGRGFIPGRVDIDERPEIVELKERFGDLEIDTIIGKNHKGAILTINDRATSRVWIR
KLSGKEAIPVAKIAVWALRKVKNLIHTITADNGKEFAKHEEIAQKLEIKFYFCKPYHSWERGANENTNGLIRQYIPKGKDFSEVTNKQIKWIENKLNNRP
RKRLGYLTPNEKFKQIINQNSVAFAS
Blast result :
Comments
Note that IS4400 (Robillard et al., 1985) is closely related (maybe identical) to IS4351, is not and will not be sequenced (M. Malamy, personal communication). It originates from Bacteroides fragilis (Tn4400 from pBFTM10), is 1.15 kb and is active.
References
1] Robillard, N.J., Tally, F.P., and Malamy, M.H. (1985) J. Bacteriol. 164, 1248-1255.
2] Smith, C.J., and Gonda, M.A. (1985) Plasmid 13, 182-192.
3] Shoemaker, N.J., Guthrie, E.P., Salyers, A.A., and Gardner, J.F. (1985) J. Bavteriol. 162, 626-632.
4] Shoemaker, N.B., Getty, C., Gardner, J.F., and Salyers, A.A. (1986) J. Bacteriol. 165, 929-936.
5] Smith, C.J., and Spiegel, H. (1987) J. Bacteriol. 169, 3450-3457.
6] Rasmussen, J.L., Odelson, D.A., and Macrina, F.L. (1987) J. Bacteriol. 169, 3573- 3580.
7] Smith, C.J. (1987) J. Bacteriol. 169, 4589-4596.
8] Hwa, V., Shoemaker, N.B., and Salyers, A.A. (1988) J. Bacteriol. 170, 449-451.
9] Hoover, C.I., Abarbarchuck, E., Ng, C.Y., and Felton, J.R. (1992) Plasmid 27, 246-250.
10] Smith, C.J., Owen, C., and Kirby, L. (1992) Mol. Microbiol. 6, 2287-2297.
11] Malamy, M.H. (1994) Personal communication
2] Smith, C.J., and Gonda, M.A. (1985) Plasmid 13, 182-192.
3] Shoemaker, N.J., Guthrie, E.P., Salyers, A.A., and Gardner, J.F. (1985) J. Bavteriol. 162, 626-632.
4] Shoemaker, N.B., Getty, C., Gardner, J.F., and Salyers, A.A. (1986) J. Bacteriol. 165, 929-936.
5] Smith, C.J., and Spiegel, H. (1987) J. Bacteriol. 169, 3450-3457.
6] Rasmussen, J.L., Odelson, D.A., and Macrina, F.L. (1987) J. Bacteriol. 169, 3573- 3580.
7] Smith, C.J. (1987) J. Bacteriol. 169, 4589-4596.
8] Hwa, V., Shoemaker, N.B., and Salyers, A.A. (1988) J. Bacteriol. 170, 449-451.
9] Hoover, C.I., Abarbarchuck, E., Ng, C.Y., and Felton, J.R. (1992) Plasmid 27, 246-250.
10] Smith, C.J., Owen, C., and Kirby, L. (1992) Mol. Microbiol. 6, 2287-2297.
11] Malamy, M.H. (1994) Personal communication