IS4321

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
U67194 ND Enterobacter aerogenes
Enterobacter aerogenes plasmid pR751
Salmonella enterica serovar Typhi CT18 plasmid pHCM1
Klebsiella pneumoniae plasmid pRMH760
Serratia marcescens plasmid pDU1358
DNA section
IS Length : 1327 bp

Ends


IR Length : 11/12

IRL : taatgagATGGTCACTCCTTCCTTCCCGGTACTATGCTGAGGATAGGCTT
IRR : ----tctATGGTCACTCCCGTTTTTGCAACACCGATTTTGACGATAAATT

Insertion site


Left flankDirect repeatRight flankDR Length
GTGCTTTATTTTCCGTTTTC0
TGCTTTATTTTCCGTTTTCG0

DNA sequence

TAATGAGATGGTCACTCCTTCCTTCCCGGTACTATGCTGAGGATAGGCTTTCATTCGGAGAACTATCATGGAAAACATTGCGCTCATTGGTATCGATCTG
GGTAAAAACTCTTTCCATATTCATTGCCAAGATCGTCGCGGCAAGGCTGTTTACCGTAAAAAATTTACACGGCCAAAGTTAATCGAATTTTTGGCGACAT
GCCCCGCTACAACCATCGCAATGGAAGCCTGTGGTGGCTCTCACTTTATGGCACGCAAGTTGGAAGAGTTGGGGCATTTTCCTAAGCTGATATCACCACA
ATTTGTCCGTCCATTCGTTAAAAGTAACAAAAACGACTTTGTCGACGCCGAAGCTATTTGTGAAGCTGCATCGCGTCCGTCTATGCGTTTTGTACAGCCC
AGAACTGAATCTCAGCAGGCAATGCGTGCGCTGCATCGTGTCCGTGAATCCCTGGTTCAGGATAAGGTAAAAACAACCAATCAGATGCATGCTTTTCTGC
TGGAATTTGGCATCAGCGTTCCACGAGGAGCTGCCGTTATTAGCCGACTGAGTACCCTTCTTGAGGACAATAGTTTGCCTCTATACCTCAGCCAGTTATT
GCTGAAATTACAACAGCATTATCACTATCTTGTTGAGCAGATTAAAGATTTGGAATCCCAGTTGAAACGAAAGTTGGACGAAGATGAGATTGGACAGCGC
TTGCTGAGCATTCCCTGCGTCGGAACACTGACAGCGAGTACTATTTCAACTGAGATTGGCGACGGGAAGCAGTACGCCAGCAGTCGTGACTTTGCGGCGG
CAACAGGGCTAGTGCCTCGACAGTACAGCACGGGAGGTCGGACGACATTGCTGGGAATTAGTAAGCGAGGTAACAAAAAGATCCGAACTTTGTTGGTTCA
ATGTGCCAGGGTATTCATACAAAAACTGGAACACCAGTCTGGCAAATTGGCCGATTGGGTCAGGGATCTACTGTGTAGGAAAAGCAACTTTGTCGTCACT
TGTGCTCTGGCAAACAAGCTGGCCAGAATAGCCTGGGCCCTAACGGCACGACAGCAAACTTATGTAGCATAACGGCAGAAATACACCGGTTTAAAGAATC
ACTGATCTGGTTTTGCGAATACTGATATTGATGATACTAACGGCCCACCGGCCTGTTGAGGAACCTGTAAAACGGAAAGGCTCATTGAAGCCGTATATTT
TCTGGAGGTTCATCAGGCGCGGAACTCATCGAGGCGCGGGAATAAAATCCCATTCAGACGCCGGATAGATTCAAGCAAGCCAATTTATCGTCAAAATCGG
TGTTGCAAAAACGGGAGTGACCATAGA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1005 bp334 aa681072+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MENIALIGIDLGKNSFHIHCQDRRGKAVYRKKFTRPKLIEFLATCPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEA
ASRPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLSTLLEDNSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLK
RKLDEDEIGQRLLSIPCVGTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGKLADWVRD
LLCRKSNFVVTCALANKLARIAWALTARQQTYVA

 

Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.

IS4321L and IS4321R are 97% identical and are located 1943 bp apart, in opposite orientation on the 53-kb plasmid R751 from Enterobacter aerogenes (Thomas, 1996).

All known copies are found in the same orientation in a 38 bp TIR of a Tn21-family transposon. They are inserted on one side of base 22 from the outer end of the transposon.

IS4321L and IS4321R are 93% identical to IS5075 and the transposase proteins are 98.5% identical. The transposase of IS4321L is also 70, 66 and 40% identical to those of ISSfl8, IS1328 and IS1111, respectively.

IS4321 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Thomas, C.M. (1996) EMBL Database Rel. 49.
2] Thorsted et al. (1998) J. Mol. Biol. 282, 969-990
3] Wain et al. (2003) Antimicrob. Agents Chemeother. 47, 2732-2739
4] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384