IS4321
- Family IS110
- Group IS1111
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
U67194 | ND | Enterobacter aerogenes | Enterobacter aerogenes plasmid pR751 Salmonella enterica serovar Typhi CT18 plasmid pHCM1 Klebsiella pneumoniae plasmid pRMH760 Serratia marcescens plasmid pDU1358 |
DNA section
IS Length : 1327 bp
Ends
IR Length : 11/12
IRL : taatgagATGGTCACTCCTTCCTTCCCGGTACTATGCTGAGGATAGGCTT
IRR : ----tctATGGTCACTCCCGTTTTTGCAACACCGATTTTGACGATAAATT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GTGCTTTATT | TTCCGTTTTC | 0 | |
TGCTTTATTT | TCCGTTTTCG | 0 |
DNA sequence
TAATGAGATGGTCACTCCTTCCTTCCCGGTACTATGCTGAGGATAGGCTTTCATTCGGAGAACTATCATGGAAAACATTGCGCTCATTGGTATCGATCTG
GGTAAAAACTCTTTCCATATTCATTGCCAAGATCGTCGCGGCAAGGCTGTTTACCGTAAAAAATTTACACGGCCAAAGTTAATCGAATTTTTGGCGACAT
GCCCCGCTACAACCATCGCAATGGAAGCCTGTGGTGGCTCTCACTTTATGGCACGCAAGTTGGAAGAGTTGGGGCATTTTCCTAAGCTGATATCACCACA
ATTTGTCCGTCCATTCGTTAAAAGTAACAAAAACGACTTTGTCGACGCCGAAGCTATTTGTGAAGCTGCATCGCGTCCGTCTATGCGTTTTGTACAGCCC
AGAACTGAATCTCAGCAGGCAATGCGTGCGCTGCATCGTGTCCGTGAATCCCTGGTTCAGGATAAGGTAAAAACAACCAATCAGATGCATGCTTTTCTGC
TGGAATTTGGCATCAGCGTTCCACGAGGAGCTGCCGTTATTAGCCGACTGAGTACCCTTCTTGAGGACAATAGTTTGCCTCTATACCTCAGCCAGTTATT
GCTGAAATTACAACAGCATTATCACTATCTTGTTGAGCAGATTAAAGATTTGGAATCCCAGTTGAAACGAAAGTTGGACGAAGATGAGATTGGACAGCGC
TTGCTGAGCATTCCCTGCGTCGGAACACTGACAGCGAGTACTATTTCAACTGAGATTGGCGACGGGAAGCAGTACGCCAGCAGTCGTGACTTTGCGGCGG
CAACAGGGCTAGTGCCTCGACAGTACAGCACGGGAGGTCGGACGACATTGCTGGGAATTAGTAAGCGAGGTAACAAAAAGATCCGAACTTTGTTGGTTCA
ATGTGCCAGGGTATTCATACAAAAACTGGAACACCAGTCTGGCAAATTGGCCGATTGGGTCAGGGATCTACTGTGTAGGAAAAGCAACTTTGTCGTCACT
TGTGCTCTGGCAAACAAGCTGGCCAGAATAGCCTGGGCCCTAACGGCACGACAGCAAACTTATGTAGCATAACGGCAGAAATACACCGGTTTAAAGAATC
ACTGATCTGGTTTTGCGAATACTGATATTGATGATACTAACGGCCCACCGGCCTGTTGAGGAACCTGTAAAACGGAAAGGCTCATTGAAGCCGTATATTT
TCTGGAGGTTCATCAGGCGCGGAACTCATCGAGGCGCGGGAATAAAATCCCATTCAGACGCCGGATAGATTCAAGCAAGCCAATTTATCGTCAAAATCGG
TGTTGCAAAAACGGGAGTGACCATAGA
GGTAAAAACTCTTTCCATATTCATTGCCAAGATCGTCGCGGCAAGGCTGTTTACCGTAAAAAATTTACACGGCCAAAGTTAATCGAATTTTTGGCGACAT
GCCCCGCTACAACCATCGCAATGGAAGCCTGTGGTGGCTCTCACTTTATGGCACGCAAGTTGGAAGAGTTGGGGCATTTTCCTAAGCTGATATCACCACA
ATTTGTCCGTCCATTCGTTAAAAGTAACAAAAACGACTTTGTCGACGCCGAAGCTATTTGTGAAGCTGCATCGCGTCCGTCTATGCGTTTTGTACAGCCC
AGAACTGAATCTCAGCAGGCAATGCGTGCGCTGCATCGTGTCCGTGAATCCCTGGTTCAGGATAAGGTAAAAACAACCAATCAGATGCATGCTTTTCTGC
TGGAATTTGGCATCAGCGTTCCACGAGGAGCTGCCGTTATTAGCCGACTGAGTACCCTTCTTGAGGACAATAGTTTGCCTCTATACCTCAGCCAGTTATT
GCTGAAATTACAACAGCATTATCACTATCTTGTTGAGCAGATTAAAGATTTGGAATCCCAGTTGAAACGAAAGTTGGACGAAGATGAGATTGGACAGCGC
TTGCTGAGCATTCCCTGCGTCGGAACACTGACAGCGAGTACTATTTCAACTGAGATTGGCGACGGGAAGCAGTACGCCAGCAGTCGTGACTTTGCGGCGG
CAACAGGGCTAGTGCCTCGACAGTACAGCACGGGAGGTCGGACGACATTGCTGGGAATTAGTAAGCGAGGTAACAAAAAGATCCGAACTTTGTTGGTTCA
ATGTGCCAGGGTATTCATACAAAAACTGGAACACCAGTCTGGCAAATTGGCCGATTGGGTCAGGGATCTACTGTGTAGGAAAAGCAACTTTGTCGTCACT
TGTGCTCTGGCAAACAAGCTGGCCAGAATAGCCTGGGCCCTAACGGCACGACAGCAAACTTATGTAGCATAACGGCAGAAATACACCGGTTTAAAGAATC
ACTGATCTGGTTTTGCGAATACTGATATTGATGATACTAACGGCCCACCGGCCTGTTGAGGAACCTGTAAAACGGAAAGGCTCATTGAAGCCGTATATTT
TCTGGAGGTTCATCAGGCGCGGAACTCATCGAGGCGCGGGAATAAAATCCCATTCAGACGCCGGATAGATTCAAGCAAGCCAATTTATCGTCAAAATCGG
TGTTGCAAAAACGGGAGTGACCATAGA
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1005 bp | 334 aa | 68 | 1072 | + | No |
Chemistry : DEDD
ORF sequence :
MENIALIGIDLGKNSFHIHCQDRRGKAVYRKKFTRPKLIEFLATCPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEA
ASRPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLSTLLEDNSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLK
RKLDEDEIGQRLLSIPCVGTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGKLADWVRD
LLCRKSNFVVTCALANKLARIAWALTARQQTYVA
ASRPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLSTLLEDNSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLK
RKLDEDEIGQRLLSIPCVGTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGKLADWVRD
LLCRKSNFVVTCALANKLARIAWALTARQQTYVA
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
IS4321L and IS4321R are 97% identical and are located 1943 bp apart, in opposite orientation on the 53-kb plasmid R751 from Enterobacter aerogenes (Thomas, 1996).
All known copies are found in the same orientation in a 38 bp TIR of a Tn21-family transposon. They are inserted on one side of base 22 from the outer end of the transposon.
IS4321L and IS4321R are 93% identical to IS5075 and the transposase proteins are 98.5% identical. The transposase of IS4321L is also 70, 66 and 40% identical to those of ISSfl8, IS1328 and IS1111, respectively.
IS4321 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
IS4321L and IS4321R are 97% identical and are located 1943 bp apart, in opposite orientation on the 53-kb plasmid R751 from Enterobacter aerogenes (Thomas, 1996).
All known copies are found in the same orientation in a 38 bp TIR of a Tn21-family transposon. They are inserted on one side of base 22 from the outer end of the transposon.
IS4321L and IS4321R are 93% identical to IS5075 and the transposase proteins are 98.5% identical. The transposase of IS4321L is also 70, 66 and 40% identical to those of ISSfl8, IS1328 and IS1111, respectively.
IS4321 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Thomas, C.M. (1996) EMBL Database Rel. 49.
2] Thorsted et al. (1998) J. Mol. Biol. 282, 969-990
3] Wain et al. (2003) Antimicrob. Agents Chemeother. 47, 2732-2739
4] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Thorsted et al. (1998) J. Mol. Biol. 282, 969-990
3] Wain et al. (2003) Antimicrob. Agents Chemeother. 47, 2732-2739
4] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384