IS421

  • Family IS4
  • Group IS231
MGE type ISRelated element(s) :
Isoform Synonym(s) IS186
Accession numberTranspositionOriginHost
Y07501 Y Escherichia coli
Escherichia coli C
Escherichia coli B
Escherichia coli JA221
DNA section
IS Length : 1342 bp

Ends


IR Length : 23

IRL : CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACG
IRR : CATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGG

Insertion site


Left flankDirect repeatRight flankDR Length
CCCGCACCGGGGAGACCTCCCCCGCCATCAG11

DNA sequence

CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATT
GGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGG
CTTACGGCCCCGGGGGGATGTCGTCATTACGTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGCTCTCCTGAAGCGGCT
GCGGAATGCCGCCGACTGGTTTGGCATACTTGCCGCACAAACACTTGCTGTACGCGCCGCAGTTACGGGTTGTACAAGCGGAAAGAGATTGCGTCTTGTC
GATGGAACAGCAATCAGTGCGCCCGGGGGCGGCAGCGCTGAATGGCGACTACATATGGGATATGATCCTCATACCTGTCAGTTCACTGATTTTGAGCTAA
CCGACAGCAGAGACGCTGAACGGCTGGACCGATTTGCGCAAACGGCAGACGAGATACGCATTGCTGACCGGGGATTCGGTTCGCGTCCCGAATGTATCCG
CTCACTTGCTTTTGGAGAAGCTGATTATATCGTCCGGGTTCACTGGCGAGGATTGCGCTGGTTAACTGCAGAAGGAATGCGCTTTGACATGATGGGTTTT
CTGCGCGGGCTGGATTGCGGTAAGAACGGTGAAACCACTGTAATGATAGGCAATTCAGGTAATAAAAAAGCCGGAGCTCCCTTTCCGGCACGTCTCATTG
CCGTATCACTTCCTCCCGAAAAAGCATTAATCAGTAAAACCCGACTGCTCAGCGAGAATCGTCGAAAAGGACGAGTAGTTCAGGCGGAAACGCTGGAAGC
AGCGGGCCATGTGCTATTGCTAACATCATTACCGGAAGATGAATATTCAGCAGAGCAAGTGGCTGATTGTTACCGTCTGCGATGGCAAATTGAACTGGCT
TTTAAGCGGCTCAAAAGTTTGCTGCACCTGGATGCTTTGCGTGCAAAGGAACCTGAACTCGCGAAAGCGTGGATATTTGCTAATCTACTCGCCGCATTTT
TAATTGACGACATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCA
TCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCG
TCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1116 bp371 aa1151230+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLLRLGLAYGPGGMSSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVR
AAVTGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFAQTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGL
RWLTAEGMRFDMMGFLRGLDCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQAETLEAAGHVLLLTSLPEDEYSAE
QVADCYRLRWQIELAFKRLKSLLHLDALRAKEPELAKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN

 

Blast result :
Comments
IS421 was serendipitously discovered during studies aiming at enhancing the expression of the Thermus thermophilus trpG gene in Escherichia coli, as an insertion downstream the trpG gene. Five copies of IS421 were found in Escherichia coli C and 4 in B and K-12 (Sato et al., 1989). IS421 DNA sequence is 99 % identical to that of IS186.
References
1] Sato, S., Nakada, Y., and Shiratsuchi, A. (1989) FEBS Lett. 249, 21-26
2] Binns, M. (1993) Nucl. Acids Res. 21, 779.